CN181955

Overview
NameCN181955
Unique NameCN181955
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:158..780. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_SOLLC (S-adenosylmethionine synthetase 1 OS=Solanum lycopersicum GN=SAM1 PE=2 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 2.704e-88
Identity = 149/158 (94.30%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTN+VMVFGEITTKA VDYEKIVRDTCR+IGFVSDDVGLDAD+CKVLV IEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNLVMVFGEITTKAIVDYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 40.817 bits (94), Expect = 2.704e-88
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVTVEY
Sbjct:  160 TCAWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_BRAJU (S-adenosylmethionine synthetase 2 OS=Brassica juncea GN=MSAMS2 PE=2 SV=1)

HSP 1 Score: 305.064 bits (780), Expect = 2.704e-88
Identity = 145/158 (91.77%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ME+FLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVR+TCR IGF+SDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPLSHVLATKLGA+LTEVRKN
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCREIGFISDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGAKLTEVRKN 158

HSP 2 Score: 41.9726 bits (97), Expect = 2.704e-88
Identity = 16/18 (88.89%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC+WLRPDGKTQVTVEY+
Sbjct:  160 TCAWLRPDGKTQVTVEYF 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_LITCN (S-adenosylmethionine synthetase OS=Litchi chinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 2.704e-88
Identity = 145/158 (91.77%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCT+TNMVMVFGEITTKA VDYE+IVRDTCRSIGF SDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTRTNMVMVFGEITTKANVDYEQIVRDTCRSIGFTSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 43.5134 bits (101), Expect = 2.704e-88
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC+WLRPDGKTQVTVEYY
Sbjct:  160 TCAWLRPDGKTQVTVEYY 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_SOLTU (S-adenosylmethionine synthetase 1 OS=Solanum tuberosum GN=METK1 PE=2 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 4.602e-88
Identity = 149/158 (94.30%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTN+VMVFGEITTKA VDYEKIVRDTCR+IGFVSDDVGLDAD+CKVLV IEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNLVMVFGEITTKAIVDYEKIVRDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 4.602e-88
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_ORYSJ (S-adenosylmethionine synthetase 3 OS=Oryza sativa subsp. japonica GN=METK3 PE=2 SV=1)

HSP 1 Score: 306.99 bits (785), Expect = 6.003e-88
Identity = 147/159 (92.45%), Postives = 154/159 (96.86%), Query Frame = 2 
Query:   89 KMETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            +++TFLFTSESVNEGHPDKLCDQISDAVLDACL +DPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGFVS+DVGLDA+HCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    3 EVDTFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDAEHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 161

HSP 2 Score: 38.891 bits (89), Expect = 6.003e-88
Identity = 15/16 (93.75%), Postives = 16/16 (100.00%), Query Frame = 3 
Query:  573 CSWLRPDGKTQVTVEY 620
            C+WLRPDGKTQVTVEY
Sbjct:  164 CAWLRPDGKTQVTVEY 179
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_SUASA (S-adenosylmethionine synthetase 2 OS=Suaeda salsa GN=METK2 PE=2 SV=1)

HSP 1 Score: 305.064 bits (780), Expect = 6.003e-88
Identity = 146/158 (92.41%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ME+FLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA +DYEKIVRDTCRSIGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPED+GAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKANIDYEKIVRDTCRSIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEDVGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 40.817 bits (94), Expect = 6.003e-88
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVTVEY
Sbjct:  160 TCAWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_POPTR (S-adenosylmethionine synthetase 2 OS=Populus trichocarpa GN=METK2 PE=2 SV=1)

HSP 1 Score: 305.834 bits (782), Expect = 6.003e-88
Identity = 147/158 (93.04%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVR TCRSIGFVSDDVGLDAD C VLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRSTCRSIGFVSDDVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 6.003e-88
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK4_VITVI (S-adenosylmethionine synthetase 4 OS=Vitis vinifera GN=METK4 PE=3 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 6.003e-88
Identity = 144/158 (91.14%), Postives = 154/158 (97.47%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTN+VM+FGEITTKA +DYEKIVRDTCR++GFVS+DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNLVMIFGEITTKANLDYEKIVRDTCRAVGFVSEDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 41.2022 bits (95), Expect = 6.003e-88
Identity = 16/18 (88.89%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC WLRPDGKTQVTVEY+
Sbjct:  160 TCPWLRPDGKTQVTVEYF 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_SOLLC (S-adenosylmethionine synthetase 2 OS=Solanum lycopersicum GN=SAM2 PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 6.003e-88
Identity = 145/158 (91.77%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPESKVACETCTKTN+VMVFGEITTKA +DYEKIVRDTCR IGFVS DVGLDAD+C+VLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPESKVACETCTKTNLVMVFGEITTKANIDYEKIVRDTCREIGFVSPDVGLDADNCRVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 42.743 bits (99), Expect = 6.003e-88
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TCSWLRPDGKTQVTVEY+
Sbjct:  160 TCSWLRPDGKTQVTVEYH 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_ORYSJ (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. japonica GN=SAM1 PE=2 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 7.830e-88
Identity = 145/158 (91.77%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ++TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVR+TCR+IGFVS DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    4 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 161

HSP 2 Score: 40.817 bits (94), Expect = 7.830e-88
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVTVEY
Sbjct:  163 TCAWLRPDGKTQVTVEY 179
The following BLAST results are available for this feature:
BLAST of CN181955 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_SOLLC2.704e-8894.30S-adenosylmethionine synthetase 1 OS=Solanum lycop... [more]
METK2_BRAJU2.704e-8891.77S-adenosylmethionine synthetase 2 OS=Brassica junc... [more]
METK_LITCN2.704e-8891.77S-adenosylmethionine synthetase OS=Litchi chinensi... [more]
METK1_SOLTU4.602e-8894.30S-adenosylmethionine synthetase 1 OS=Solanum tuber... [more]
METK3_ORYSJ6.003e-8892.45S-adenosylmethionine synthetase 3 OS=Oryza sativa ... [more]
METK2_SUASA6.003e-8892.41S-adenosylmethionine synthetase 2 OS=Suaeda salsa ... [more]
METK2_POPTR6.003e-8893.04S-adenosylmethionine synthetase 2 OS=Populus trich... [more]
METK4_VITVI6.003e-8891.14S-adenosylmethionine synthetase 4 OS=Vitis vinifer... [more]
METK2_SOLLC6.003e-8891.77S-adenosylmethionine synthetase 2 OS=Solanum lycop... [more]
METK1_ORYSJ7.830e-8891.77S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001L15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001L15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181955 ID=CN181955; Name=CN181955; organism=Citrus sinensis; type=EST; length=625bp
GAGGATTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCT
TAGATCTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACC
TTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTG
CGACCAGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTG
AGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTC
TTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCG
CGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACG
CCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATT
GCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGC
TGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAAT
TTATGCCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACG
GAGGTTAGGAAGAATAGTAACCTGCTCTTGGCTAAGGCCCGATGGGAAGA
CCCAGGTCACTGTTGAGTACTACAA
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