CN181955

Overview
NameCN181955
Unique NameCN181955
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_ORYSI (S-adenosylmethionine synthetase 1 OS=Oryza sativa subsp. indica GN=SAM1 PE=2 SV=2)

HSP 1 Score: 304.679 bits (779), Expect = 7.830e-88
Identity = 145/158 (91.77%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ++TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVR+TCR+IGFVS DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    4 LDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRETCRNIGFVSADVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 161

HSP 2 Score: 40.817 bits (94), Expect = 7.830e-88
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVTVEY
Sbjct:  163 TCAWLRPDGKTQVTVEY 179
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_POPDE (S-adenosylmethionine synthetase OS=Populus deltoides GN=METK PE=2 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 7.830e-88
Identity = 146/157 (92.99%), Postives = 151/157 (96.18%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHF+KRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRNIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHFSKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 159

HSP 2 Score: 43.5134 bits (101), Expect = 7.830e-88
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC+WLRPDGKTQVTVEYY
Sbjct:  161 TCAWLRPDGKTQVTVEYY 178
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_POPTR (S-adenosylmethionine synthetase 3 OS=Populus trichocarpa GN=METK3 PE=2 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 7.830e-88
Identity = 146/157 (92.99%), Postives = 151/157 (96.18%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGHF+KRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRNIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHFSKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 159

HSP 2 Score: 43.5134 bits (101), Expect = 7.830e-88
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC+WLRPDGKTQVTVEYY
Sbjct:  161 TCAWLRPDGKTQVTVEYY 178
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_TOBAC (S-adenosylmethionine synthetase 2 OS=Nicotiana tabacum GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 7.831e-88
Identity = 145/158 (91.77%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLAT+LGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATQLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 7.831e-88
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_PINCO (S-adenosylmethionine synthetase 2 OS=Pinus contorta GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 1.021e-87
Identity = 146/158 (92.41%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLTQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 39.6614 bits (91), Expect = 1.021e-87
Identity = 15/17 (88.24%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVT+EY
Sbjct:  160 TCPWLRPDGKTQVTIEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_MEDFA (S-adenosylmethionine synthetase OS=Medicago falcata GN=SAMS PE=2 SV=1)

HSP 1 Score: 303.138 bits (775), Expect = 2.267e-87
Identity = 145/157 (92.36%), Postives = 153/157 (97.45%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFL+TSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVR+TCR+IGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    4 ETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 160

HSP 2 Score: 40.817 bits (94), Expect = 2.267e-87
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC WLRPDGKTQVT+EY+
Sbjct:  162 TCPWLRPDGKTQVTIEYF 179
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_PICSI (S-adenosylmethionine synthetase 2 OS=Picea sitchensis GN=METK2 PE=2 SV=1)

HSP 1 Score: 304.294 bits (778), Expect = 2.267e-87
Identity = 145/158 (91.77%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA +DYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLIQDPDSKVACETCTKTNMVMVFGEITTKADIDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 39.6614 bits (91), Expect = 2.267e-87
Identity = 15/17 (88.24%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVT+EY
Sbjct:  160 TCPWLRPDGKTQVTIEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_SOLLC (S-adenosylmethionine synthetase 3 OS=Solanum lycopersicum GN=SAM3 PE=2 SV=1)

HSP 1 Score: 303.523 bits (776), Expect = 2.958e-87
Identity = 145/158 (91.77%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 2.958e-87
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_HORVU (S-adenosylmethionine synthetase 2 OS=Hordeum vulgare GN=SAM2 PE=2 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 3.858e-87
Identity = 144/157 (91.72%), Postives = 151/157 (96.18%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE++GAGDQG MFGYATDET E MPL+H+LATKLGARLTEVRKN
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRDIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEVGAGDQGIMFGYATDETPELMPLTHMLATKLGARLTEVRKN 160

HSP 2 Score: 40.4318 bits (93), Expect = 3.858e-87
Identity = 15/17 (88.24%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVT+EY
Sbjct:  162 TCAWLRPDGKTQVTIEY 178
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_HORVU (S-adenosylmethionine synthetase 1 OS=Hordeum vulgare GN=SAM1 PE=2 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 3.858e-87
Identity = 144/157 (91.72%), Postives = 151/157 (96.18%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE++GAGDQG MFGYATDET E MPL+H+LATKLGARLTEVRKN
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRDIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEVGAGDQGIMFGYATDETPELMPLTHMLATKLGARLTEVRKN 160

HSP 2 Score: 40.4318 bits (93), Expect = 3.858e-87
Identity = 15/17 (88.24%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVT+EY
Sbjct:  162 TCAWLRPDGKTQVTIEY 178
The following BLAST results are available for this feature:
BLAST of CN181955 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_ORYSI7.830e-8891.77S-adenosylmethionine synthetase 1 OS=Oryza sativa ... [more]
METK_POPDE7.830e-8892.99S-adenosylmethionine synthetase OS=Populus deltoid... [more]
METK3_POPTR7.830e-8892.99S-adenosylmethionine synthetase 3 OS=Populus trich... [more]
METK2_TOBAC7.831e-8891.77S-adenosylmethionine synthetase 2 OS=Nicotiana tab... [more]
METK2_PINCO1.021e-8792.41S-adenosylmethionine synthetase 2 OS=Pinus contort... [more]
METK_MEDFA2.267e-8792.36S-adenosylmethionine synthetase OS=Medicago falcat... [more]
METK2_PICSI2.267e-8791.77S-adenosylmethionine synthetase 2 OS=Picea sitchen... [more]
METK3_SOLLC2.958e-8791.77S-adenosylmethionine synthetase 3 OS=Solanum lycop... [more]
METK2_HORVU3.858e-8791.72S-adenosylmethionine synthetase 2 OS=Hordeum vulga... [more]
METK1_HORVU3.858e-8791.72S-adenosylmethionine synthetase 1 OS=Hordeum vulga... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001L15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001L15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181955 ID=CN181955; Name=CN181955; organism=Citrus sinensis; type=EST; length=625bp
GAGGATTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCT
TAGATCTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACC
TTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTG
CGACCAGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTG
AGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTC
TTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCG
CGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACG
CCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATT
GCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGC
TGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAAT
TTATGCCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACG
GAGGTTAGGAAGAATAGTAACCTGCTCTTGGCTAAGGCCCGATGGGAAGA
CCCAGGTCACTGTTGAGTACTACAA
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