CN181955

Overview
NameCN181955
Unique NameCN181955
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:158..780. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_VITVI (S-adenosylmethionine synthetase 1 OS=Vitis vinifera GN=METK1 PE=3 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 5.033e-87
Identity = 144/158 (91.14%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 5.033e-87
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_PETHY (S-adenosylmethionine synthetase 2 OS=Petunia hybrida GN=SAM2 PE=2 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 5.033e-87
Identity = 144/158 (91.14%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKT+MVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTSMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 5.033e-87
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_ACTCH (S-adenosylmethionine synthetase 2 OS=Actinidia chinensis GN=SAM2 PE=2 SV=1)

HSP 1 Score: 302.753 bits (774), Expect = 5.033e-87
Identity = 143/159 (89.94%), Postives = 153/159 (96.23%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNS 568
            ME+FLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKAKV+YEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH +K+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN+
Sbjct:    1 MESFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNN 159

HSP 2 Score: 40.0466 bits (92), Expect = 5.033e-87
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_DAUCA (S-adenosylmethionine synthetase 1 OS=Daucus carota GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 299.671 bits (766), Expect = 6.560e-87
Identity = 144/158 (91.14%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFL+TSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETC+KTNMVMVFGEITTKA VDYEKIV  TCR IGFVSDDVGLDAD+CKVLV IEQQSPDIAQGVHGH TKRPEDIGAGDQGHMFGYATDET E MPLSHVLATKLGA+LTEVRKN
Sbjct:    1 MDTFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCSKTNMVMVFGEITTKANVDYEKIVHKTCRDIGFVSDDVGLDADNCKVLVQIEQQSPDIAQGVHGHLTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKLGAKLTEVRKN 158

HSP 2 Score: 42.743 bits (99), Expect = 6.560e-87
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC WLRPDGKTQVTVEYY
Sbjct:  160 TCPWLRPDGKTQVTVEYY 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_PICSI (S-adenosylmethionine synthetase 3 OS=Picea sitchensis GN=METK3 PE=2 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 6.565e-87
Identity = 145/158 (91.77%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAG QGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLTQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGYQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.4318 bits (93), Expect = 6.565e-87
Identity = 15/17 (88.24%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVT+EY
Sbjct:  160 TCAWLRPDGKTQVTIEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK5_POPTR (S-adenosylmethionine synthetase 5 OS=Populus trichocarpa GN=METK5 PE=2 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 6.565e-87
Identity = 145/157 (92.36%), Postives = 150/157 (95.54%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGF S DVGLDAD+CKVLVNIEQQS DIAQGVHGHF+KRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    3 ETFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRNIGFTSADVGLDADNCKVLVNIEQQSSDIAQGVHGHFSKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 159

HSP 2 Score: 43.5134 bits (101), Expect = 6.565e-87
Identity = 17/18 (94.44%), Postives = 18/18 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC+WLRPDGKTQVTVEYY
Sbjct:  161 TCAWLRPDGKTQVTVEYY 178
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_PETHY (S-adenosylmethionine synthetase 1 OS=Petunia hybrida GN=SAM1 PE=2 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 6.565e-87
Identity = 144/158 (91.14%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HV ATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVWATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 6.565e-87
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_TRIMO (S-adenosylmethionine synthetase 1 OS=Triticum monococcum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 8.563e-87
Identity = 146/157 (92.99%), Postives = 150/157 (95.54%), Query Frame = 2 
Query:   95 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ETFLFTSESVNEGHPDKLCDQ+SDAVLDACL QD +SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQG MFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDADSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRNIGFISDDVGLDADRCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGIMFGYATDETPELMPLSHVLATKLGARLTEVRKN 160

HSP 2 Score: 40.817 bits (94), Expect = 8.563e-87
Identity = 16/17 (94.12%), Postives = 17/17 (100.00%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC+WLRPDGKTQVTVEY
Sbjct:  162 TCAWLRPDGKTQVTVEY 178
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_MESCR (S-adenosylmethionine synthetase OS=Mesembryanthemum crystallinum GN=METK PE=2 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 8.564e-87
Identity = 145/158 (91.77%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFLFTSESVNEGHPDKLCDQISDAVLDACL QD ESKVACETCTKTN+VMVFGEITTKA VDYEKI RDTCR+IGFVSDDVGLDAD+CKVLV IEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQISDAVLDACLAQDAESKVACETCTKTNLVMVFGEITTKANVDYEKIARDTCRNIGFVSDDVGLDADNCKVLVYIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 158

HSP 2 Score: 42.743 bits (99), Expect = 8.564e-87
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC WLRPDGKTQVTVEYY
Sbjct:  160 TCPWLRPDGKTQVTVEYY 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_DAUCA (S-adenosylmethionine synthetase 2 OS=Daucus carota GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 299.286 bits (765), Expect = 8.564e-87
Identity = 145/158 (91.77%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFL TSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETC+KTNMVMVFGEITTKA VDYEKIVR TCR IGFVSDDVGLDAD+CKVLV IEQQSPDIAQGVHGH TKRPEDIGAGDQGHMFGYATDET E MPLSHVLATKLGA+LTEVRKN
Sbjct:    1 MDTFLNTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCSKTNMVMVFGEITTKANVDYEKIVRKTCRDIGFVSDDVGLDADNCKVLVQIEQQSPDIAQGVHGHLTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKLGAKLTEVRKN 158

HSP 2 Score: 42.743 bits (99), Expect = 8.564e-87
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC WLRPDGKTQVTVEYY
Sbjct:  160 TCPWLRPDGKTQVTVEYY 177
The following BLAST results are available for this feature:
BLAST of CN181955 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_VITVI5.033e-8791.14S-adenosylmethionine synthetase 1 OS=Vitis vinifer... [more]
METK2_PETHY5.033e-8791.14S-adenosylmethionine synthetase 2 OS=Petunia hybri... [more]
METK2_ACTCH5.033e-8789.94S-adenosylmethionine synthetase 2 OS=Actinidia chi... [more]
METK1_DAUCA6.560e-8791.14S-adenosylmethionine synthetase 1 OS=Daucus carota... [more]
METK3_PICSI6.565e-8791.77S-adenosylmethionine synthetase 3 OS=Picea sitchen... [more]
METK5_POPTR6.565e-8792.36S-adenosylmethionine synthetase 5 OS=Populus trich... [more]
METK1_PETHY6.565e-8791.14S-adenosylmethionine synthetase 1 OS=Petunia hybri... [more]
METK1_TRIMO8.563e-8792.99S-adenosylmethionine synthetase 1 OS=Triticum mono... [more]
METK_MESCR8.564e-8791.77S-adenosylmethionine synthetase OS=Mesembryanthemu... [more]
METK2_DAUCA8.564e-8791.77S-adenosylmethionine synthetase 2 OS=Daucus carota... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001L15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001L15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181955 ID=CN181955; Name=CN181955; organism=Citrus sinensis; type=EST; length=625bp
GAGGATTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCT
TAGATCTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACC
TTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTG
CGACCAGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTG
AGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTC
TTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCG
CGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACG
CCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATT
GCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGC
TGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAAT
TTATGCCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACG
GAGGTTAGGAAGAATAGTAACCTGCTCTTGGCTAAGGCCCGATGGGAAGA
CCCAGGTCACTGTTGAGTACTACAA
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