CN181955

Overview
NameCN181955
Unique NameCN181955
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_BIFLD (S-adenosylmethionine synthetase OS=Bifidobacterium longum (strain DJO10A) GN=metK PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 6.245e-41
Identity = 87/166 (52.41%), Postives = 108/166 (65.06%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGV---------HGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            L ++ESV EGHPDK+CDQISDA+LD  L QD  S VA ET   T + +VFGE+T++   D +  VR+T R+IG+ S +VGLDAD C V+V I +QS +I QGV              +R E  GAGDQG MFGYATDET   MPL   LA +L  RLTEVRK+  +
Sbjct:    7 LISAESVTEGHPDKVCDQISDAILDDLLAQDSSSHVAVETSAATGVFLVFGEVTSEGYCDVQSKVRETLRNIGYTSSEVGLDADSCGVVVAITEQSAEINQGVARLTDDQETAASREERYEAQGAGDQGVMFGYATDETPTLMPLPIYLAHRLAFRLTEVRKSGEV 172

HSP 2 Score: 30.4166 bits (67), Expect = 6.245e-41
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 3 
Query:  582 LRPDGKTQVTVEY 620
            LRPDGKTQVT+EY
Sbjct:  175 LRPDGKTQVTIEY 187
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_MOOTA (S-adenosylmethionine synthetase OS=Moorella thermoacetica (strain ATCC 39073) GN=metK PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 6.247e-41
Identity = 81/160 (50.62%), Postives = 105/160 (65.62%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRP-------EDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRK 562
            LFTSESV EGHPDK+ D+I+DAVLDA   +D +++VACE    T +++V G+ITT   VD  ++ R+T R IG+     G D D C V+ +I++QSPDIA GV+  + K+        E +GAGDQG MFGYAT ET EFMP+   LA  L  RL  VRK
Sbjct:    5 LFTSESVTEGHPDKIADRIADAVLDAIYAKDNQARVACECLVSTGLILVAGQITTDCYVDIPRVARETVREIGYTRAKFGFDCDTCAVITSIDEQSPDIAMGVNEAWEKKQGEARDEVETLGAGDQGMMFGYATSETPEFMPMPIALAHALTRRLAAVRK 164

HSP 2 Score: 30.0314 bits (66), Expect = 6.247e-41
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 3 
Query:  579 WLRPDGKTQVTVEY 620
            +LRPDGK+QVTVEY
Sbjct:  170 YLRPDGKSQVTVEY 183
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_DESVV (S-adenosylmethionine synthetase OS=Desulfovibrio vulgaris subsp. vulgaris (strain DP4) GN=metK PE=3 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 6.248e-41
Identity = 85/158 (53.80%), Postives = 106/158 (67.09%), Query Frame = 2 
Query:  101 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            + FTSESV EGHPDK+ DQISDAVLD  L QDP S+VACET   T M ++ GEITT+   D   +VR+T R+IG+ S ++G D   C V+ +I++QS DIAQGV        ED GAGDQG MFG+A DET+  MP     A +L  RLTEVRK+  +
Sbjct:    8 YYFTSESVTEGHPDKVADQISDAVLDVLLAQDPNSRVACETLVTTGMAVIAGEITTRGYADLPHVVRETIRNIGYNSSEMGFDWQTCAVISSIDKQSADIAQGVD---RATNEDQGAGDQGMMFGFACDETATLMPAPIYWAHQLSQRLTEVRKDGTV 162

HSP 2 Score: 26.1794 bits (56), Expect = 6.248e-41
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            T    RPDGKTQV+ EY
Sbjct:  161 TVDIFRPDGKTQVSFEY 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_DESVH (S-adenosylmethionine synthetase OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) GN=metK PE=3 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 6.248e-41
Identity = 85/158 (53.80%), Postives = 106/158 (67.09%), Query Frame = 2 
Query:  101 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            + FTSESV EGHPDK+ DQISDAVLD  L QDP S+VACET   T M ++ GEITT+   D   +VR+T R+IG+ S ++G D   C V+ +I++QS DIAQGV        ED GAGDQG MFG+A DET+  MP     A +L  RLTEVRK+  +
Sbjct:    8 YYFTSESVTEGHPDKVADQISDAVLDVLLAQDPNSRVACETLVTTGMAVIAGEITTRGYADLPHVVRETIRNIGYNSSEMGFDWQTCAVISSIDKQSADIAQGVD---RATNEDQGAGDQGMMFGFACDETATLMPAPIYWAHQLSQRLTEVRKDGTV 162

HSP 2 Score: 26.1794 bits (56), Expect = 6.248e-41
Identity = 11/17 (64.71%), Postives = 12/17 (70.59%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            T    RPDGKTQV+ EY
Sbjct:  161 TVDIFRPDGKTQVSFEY 177
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_NEIMB (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup B GN=metK PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 6.248e-41
Identity = 86/165 (52.12%), Postives = 113/165 (68.48%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDI----GAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M  +LFTSESV+EGHPDK+ DQ+SDA+LDA L QDP+++VA ET   T + ++ GEITT A+VDY K+ R+T + IG+ S ++G DA+ C V V  +QQSPDIAQGV+       E I    GAGDQG MFGYA DET   MP +   + +L  R +E+RK+  L
Sbjct:    1 MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVN-----EGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL 160

HSP 2 Score: 27.335 bits (59), Expect = 6.248e-41
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ GR+    WLRPD K Q+TV Y
Sbjct:  155 RKDGRL---PWLRPDAKAQLTVVY 175
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_NEIMA (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup A GN=metK PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 6.248e-41
Identity = 86/165 (52.12%), Postives = 113/165 (68.48%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDI----GAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M  +LFTSESV+EGHPDK+ DQ+SDA+LDA L QDP+++VA ET   T + ++ GEITT A+VDY K+ R+T + IG+ S ++G DA+ C V V  +QQSPDIAQGV+       E I    GAGDQG MFGYA DET   MP +   + +L  R +E+RK+  L
Sbjct:    1 MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVN-----EGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL 160

HSP 2 Score: 27.335 bits (59), Expect = 6.248e-41
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ GR+    WLRPD K Q+TV Y
Sbjct:  155 RKDGRL---PWLRPDAKAQLTVVY 175
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_NEIM0 (S-adenosylmethionine synthetase OS=Neisseria meningitidis serogroup C (strain 053442) GN=metK PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 6.248e-41
Identity = 86/165 (52.12%), Postives = 113/165 (68.48%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDI----GAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M  +LFTSESV+EGHPDK+ DQ+SDA+LDA L QDP+++VA ET   T + ++ GEITT A+VDY K+ R+T + IG+ S ++G DA+ C V V  +QQSPDIAQGV+       E I    GAGDQG MFGYA DET   MP +   + +L  R +E+RK+  L
Sbjct:    1 MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVN-----EGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL 160

HSP 2 Score: 27.335 bits (59), Expect = 6.248e-41
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ GR+    WLRPD K Q+TV Y
Sbjct:  155 RKDGRL---PWLRPDAKAQLTVVY 175
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_NEIG2 (S-adenosylmethionine synthetase OS=Neisseria gonorrhoeae (strain NCCP11945) GN=metK PE=3 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 6.248e-41
Identity = 86/165 (52.12%), Postives = 113/165 (68.48%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDI----GAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M  +LFTSESV+EGHPDK+ DQ+SDA+LDA L QDP+++VA ET   T + ++ GEITT A+VDY K+ R+T + IG+ S ++G DA+ C V V  +QQSPDIAQGV+       E I    GAGDQG MFGYA DET   MP +   + +L  R +E+RK+  L
Sbjct:    1 MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVN-----EGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL 160

HSP 2 Score: 27.335 bits (59), Expect = 6.248e-41
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ GR+    WLRPD K Q+TV Y
Sbjct:  155 RKDGRL---PWLRPDAKAQLTVVY 175
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_NEIG1 (S-adenosylmethionine synthetase OS=Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) GN=metK PE=3 SV=2)

HSP 1 Score: 161.384 bits (407), Expect = 6.248e-41
Identity = 86/165 (52.12%), Postives = 113/165 (68.48%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDI----GAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M  +LFTSESV+EGHPDK+ DQ+SDA+LDA L QDP+++VA ET   T + ++ GEITT A+VDY K+ R+T + IG+ S ++G DA+ C V V  +QQSPDIAQGV+       E I    GAGDQG MFGYA DET   MP +   + +L  R +E+RK+  L
Sbjct:    1 MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAETLVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPDIAQGVN-----EGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL 160

HSP 2 Score: 27.335 bits (59), Expect = 6.248e-41
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ GR+    WLRPD K Q+TV Y
Sbjct:  155 RKDGRL---PWLRPDAKAQLTVVY 175
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_DESVM (S-adenosylmethionine synthetase OS=Desulfovibrio vulgaris (strain Miyazaki F / DSM 19637) GN=metK PE=3 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 6.248e-41
Identity = 86/158 (54.43%), Postives = 104/158 (65.82%), Query Frame = 2 
Query:  101 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            + FTSESV EGHPDK+ DQISDAVLD  L QDP S+VACET   T M  + GEI TK   D  ++VR T R+IG+ S ++G D   C V+ +I++QS DIAQGV       PED GAGDQG MFG+A DET   MP     A +L  RLT+VRK+  L
Sbjct:    8 YFFTSESVTEGHPDKVADQISDAVLDVLLAQDPMSRVACETLVTTGMAFIAGEIRTKGFADLPEVVRSTIRNIGYNSSEMGFDWKTCAVISSIDKQSGDIAQGVD---RGNPEDQGAGDQGMMFGFACDETPTLMPAPIYWAHQLSQRLTQVRKDGIL 162

HSP 2 Score: 27.7202 bits (60), Expect = 6.248e-41
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  579 WLRPDGKTQVTVEY 620
            +LRPDGKTQV+ EY
Sbjct:  164 FLRPDGKTQVSFEY 177
The following BLAST results are available for this feature:
BLAST of CN181955 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_BIFLD6.245e-4152.41S-adenosylmethionine synthetase OS=Bifidobacterium... [more]
METK_MOOTA6.247e-4150.63S-adenosylmethionine synthetase OS=Moorella thermo... [more]
METK_DESVV6.248e-4153.80S-adenosylmethionine synthetase OS=Desulfovibrio v... [more]
METK_DESVH6.248e-4153.80S-adenosylmethionine synthetase OS=Desulfovibrio v... [more]
METK_NEIMB6.248e-4152.12S-adenosylmethionine synthetase OS=Neisseria menin... [more]
METK_NEIMA6.248e-4152.12S-adenosylmethionine synthetase OS=Neisseria menin... [more]
METK_NEIM06.248e-4152.12S-adenosylmethionine synthetase OS=Neisseria menin... [more]
METK_NEIG26.248e-4152.12S-adenosylmethionine synthetase OS=Neisseria gonor... [more]
METK_NEIG16.248e-4152.12S-adenosylmethionine synthetase OS=Neisseria gonor... [more]
METK_DESVM6.248e-4154.43S-adenosylmethionine synthetase OS=Desulfovibrio v... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001L15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001L15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181955 ID=CN181955; Name=CN181955; organism=Citrus sinensis; type=EST; length=625bp
GAGGATTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCT
TAGATCTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACC
TTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTG
CGACCAGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTG
AGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTC
TTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCG
CGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACG
CCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATT
GCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGC
TGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAAT
TTATGCCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACG
GAGGTTAGGAAGAATAGTAACCTGCTCTTGGCTAAGGCCCGATGGGAAGA
CCCAGGTCACTGTTGAGTACTACAA
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