CN181955

Overview
NameCN181955
Unique NameCN181955
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_PSYCK (S-adenosylmethionine synthetase OS=Psychrobacter cryohalolentis (strain K5) GN=metK PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 6.248e-41
Identity = 84/157 (53.50%), Postives = 107/157 (68.15%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            LFTSESV+EGHPDK+ DQISDA+LDA L QD  ++VACET  KT  V++ GEITT A +D E+IVRDT   IG+   D+G D + C V+  + +QSP+IAQGV       PE+ GAGDQG MFGYA++ET   MP     A +L  R +E+R+   L
Sbjct:    6 LFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVVLAGEITTTANIDVERIVRDTVNGIGYHHSDLGFDGETCAVINMLGKQSPEIAQGVD---RADPEEQGAGDQGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGEL 159

HSP 2 Score: 28.8758 bits (63), Expect = 6.248e-41
Identity = 13/24 (54.17%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            RR G +    WLRPD K QVT++Y
Sbjct:  154 RRAGEL---PWLRPDAKAQVTLKY 174
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_HAEIG (S-adenosylmethionine synthetase OS=Haemophilus influenzae (strain PittGG) GN=metK PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 6.250e-41
Identity = 86/161 (53.42%), Postives = 106/161 (65.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M ++LFTSESV+EGHPDK+ DQISDAVLD  L+QDP+++VACET  KT M +V GEITT A VD E + R     IG+   ++G D   C VL  I +QS DI QGV     + P D GAGDQG MFGYAT+ET   MP +   A +L  +  EVRK+  L
Sbjct:    1 MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVD---RENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKL 158

HSP 2 Score: 29.261 bits (64), Expect = 6.250e-41
Identity = 12/24 (50.00%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ G++   +WLRPD K+QVT++Y
Sbjct:  153 RKSGKL---AWLRPDAKSQVTLKY 173
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_HAEIE (S-adenosylmethionine synthetase OS=Haemophilus influenzae (strain PittEE) GN=metK PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 6.250e-41
Identity = 86/161 (53.42%), Postives = 106/161 (65.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M ++LFTSESV+EGHPDK+ DQISDAVLD  L+QDP+++VACET  KT M +V GEITT A VD E + R     IG+   ++G D   C VL  I +QS DI QGV     + P D GAGDQG MFGYAT+ET   MP +   A +L  +  EVRK+  L
Sbjct:    1 MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVD---RENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKL 158

HSP 2 Score: 29.261 bits (64), Expect = 6.250e-41
Identity = 12/24 (50.00%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ G++   +WLRPD K+QVT++Y
Sbjct:  153 RKSGKL---AWLRPDAKSQVTLKY 173
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_HAEI8 (S-adenosylmethionine synthetase OS=Haemophilus influenzae (strain 86-028NP) GN=metK PE=3 SV=1)

HSP 1 Score: 159.458 bits (402), Expect = 6.250e-41
Identity = 86/161 (53.42%), Postives = 106/161 (65.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            M ++LFTSESV+EGHPDK+ DQISDAVLD  L+QDP+++VACET  KT M +V GEITT A VD E + R     IG+   ++G D   C VL  I +QS DI QGV     + P D GAGDQG MFGYAT+ET   MP +   A +L  +  EVRK+  L
Sbjct:    1 MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVD---RENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKL 158

HSP 2 Score: 29.261 bits (64), Expect = 6.250e-41
Identity = 12/24 (50.00%), Postives = 19/24 (79.17%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            R+ G++   +WLRPD K+QVT++Y
Sbjct:  153 RKSGKL---AWLRPDAKSQVTLKY 173
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_AMOPS (S-adenosylmethionine synthetase OS=Amoeba proteus symbiotic bacterium GN=metK PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 6.250e-41
Identity = 88/157 (56.05%), Postives = 107/157 (68.15%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            +FTSESV+EGHPDK+ DQISDA+LDA L +DP ++VACET  KT MV+V GEITT A VD E I R+  + IG+ S D+G D   C VL  I +QS DIAQGV    TK    +GAGDQG MFGYA+ ET   MP     + +L AR  E+RKN+ L
Sbjct:    5 VFTSESVSEGHPDKIADQISDAILDAILAKDPLARVACETFVKTGMVLVGGEITTSAWVDVETITREVIKDIGYNSSDMGFDWASCSVLSAIGKQSLDIAQGVDNRETK---ILGAGDQGLMFGYASRETDVCMPAPIAYSHRLMARQAELRKNNKL 158

HSP 2 Score: 28.8758 bits (63), Expect = 6.250e-41
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  579 WLRPDGKTQVTVEY 620
            WLRPDGK Q+T++Y
Sbjct:  160 WLRPDGKCQLTLKY 173
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_BACSU (S-adenosylmethionine synthetase OS=Bacillus subtilis GN=metK PE=3 SV=2)

HSP 1 Score: 167.162 bits (422), Expect = 6.292e-41
Identity = 90/165 (54.55%), Postives = 107/165 (64.85%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRP--------EDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            LFTSESV EGHPDK+CDQISD++LD  L++DP ++VACET   T +V+V GEITT   VD  K VR T + IG+     G DA+ C VL +I++QS DIA GV      R         E IGAGDQG MFGYA +ET E MPL   LA KL  RL+EVRK   L
Sbjct:    7 LFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITTSTYVDIPKTVRQTIKEIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTMSDEEIEAIGAGDQGLMFGYACNETKELMPLPISLAHKLARRLSEVRKEDIL 171
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_STAES (S-adenosylmethionine synthetase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=metK PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 8.139e-41
Identity = 84/164 (51.22%), Postives = 105/164 (64.02%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRP-------EDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            LFTSESV EGHPDK+ DQ+SDA+LD  L+ DP ++VACET   T M ++ GEI+T   VD  K+VR+T + IG+     G D+    VL  I++QSPDIAQGV      R        E  GAGDQG MFGYATDET  +MPL   L+ +L  RL +VRK+  L
Sbjct:    7 LFTSESVTEGHPDKIADQVSDAILDEILKDDPNARVACETTVTTGMALISGEISTTTYVDIPKVVRETIKDIGYTRAKYGYDSQTMAVLTAIDEQSPDIAQGVDKALEYRNDISEEEIEATGAGDQGLMFGYATDETDTYMPLPIFLSHQLAKRLADVRKDEIL 170

HSP 2 Score: 29.6462 bits (65), Expect = 8.139e-41
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  579 WLRPDGKTQVTVEY 620
            +LRPDGK QVTVEY
Sbjct:  172 YLRPDGKVQVTVEY 185
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_STAEQ (S-adenosylmethionine synthetase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=metK PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 8.139e-41
Identity = 84/164 (51.22%), Postives = 105/164 (64.02%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRP-------EDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            LFTSESV EGHPDK+ DQ+SDA+LD  L+ DP ++VACET   T M ++ GEI+T   VD  K+VR+T + IG+     G D+    VL  I++QSPDIAQGV      R        E  GAGDQG MFGYATDET  +MPL   L+ +L  RL +VRK+  L
Sbjct:    7 LFTSESVTEGHPDKIADQVSDAILDEILKDDPNARVACETTVTTGMALISGEISTTTYVDIPKVVRETIKDIGYTRAKYGYDSQTMAVLTAIDEQSPDIAQGVDKALEYRNDISEEEIEATGAGDQGLMFGYATDETDTYMPLPIFLSHQLAKRLADVRKDEIL 170

HSP 2 Score: 29.6462 bits (65), Expect = 8.139e-41
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  579 WLRPDGKTQVTVEY 620
            +LRPDGK QVTVEY
Sbjct:  172 YLRPDGKVQVTVEY 185
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_LEIXX (S-adenosylmethionine synthetase OS=Leifsonia xyli subsp. xyli GN=metK PE=3 SV=1)

HSP 1 Score: 158.303 bits (399), Expect = 8.139e-41
Identity = 89/164 (54.27%), Postives = 105/164 (64.02%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE----DI---GAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            LFTSESV EGHPDK+CDQISD++LDA L  D  S+VA ET   T +V V GE+TTK  V+   +VRDT   IG+ S DV  D   C V V+I  QSPDIAQGV      R E    D+   GAGDQG MFG+AT ET ++MPL   LA +L  RL  VRK+  L
Sbjct:    6 LFTSESVTEGHPDKICDQISDSILDALLAVDEHSRVAVETLVTTGLVHVAGEVTTKGYVEIPALVRDTIVEIGYDSSDVSFDGTQCGVSVSIGAQSPDIAQGVDNALETRSERSQDDLDRQGAGDQGIMFGFATTETPQYMPLPIWLAHRLAERLAAVRKDGTL 169

HSP 2 Score: 30.0314 bits (66), Expect = 8.139e-41
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            T  +LRPDGKTQVT+ Y
Sbjct:  168 TLGYLRPDGKTQVTIGY 184
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_PSYA2 (S-adenosylmethionine synthetase OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=metK PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 8.141e-41
Identity = 84/157 (53.50%), Postives = 107/157 (68.15%), Query Frame = 2 
Query:  104 LFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNSNL 574
            LFTSESV+EGHPDK+ DQISDA+LDA L QD  ++VACET  KT  V++ GEITT A +D E+IVRDT   IG+   D+G D + C V+  + +QSP+IAQGV       PE+ GAGDQG MFGYA++ET   MP     A +L  R +E+R+   L
Sbjct:    6 LFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVILAGEITTTANIDVERIVRDTVNGIGYHHSDLGFDGETCAVINMLGKQSPEIAQGVD---RADPEEQGAGDQGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGEL 159

HSP 2 Score: 28.4906 bits (62), Expect = 8.141e-41
Identity = 12/24 (50.00%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  549 RRLGRIVTCSWLRPDGKTQVTVEY 620
            RR G +    WLRPD K Q+T++Y
Sbjct:  154 RRAGEL---PWLRPDAKAQITLKY 174
The following BLAST results are available for this feature:
BLAST of CN181955 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_PSYCK6.248e-4153.50S-adenosylmethionine synthetase OS=Psychrobacter c... [more]
METK_HAEIG6.250e-4153.42S-adenosylmethionine synthetase OS=Haemophilus inf... [more]
METK_HAEIE6.250e-4153.42S-adenosylmethionine synthetase OS=Haemophilus inf... [more]
METK_HAEI86.250e-4153.42S-adenosylmethionine synthetase OS=Haemophilus inf... [more]
METK_AMOPS6.250e-4156.05S-adenosylmethionine synthetase OS=Amoeba proteus ... [more]
METK_BACSU6.292e-4154.55S-adenosylmethionine synthetase OS=Bacillus subtil... [more]
METK_STAES8.139e-4151.22S-adenosylmethionine synthetase OS=Staphylococcus ... [more]
METK_STAEQ8.139e-4151.22S-adenosylmethionine synthetase OS=Staphylococcus ... [more]
METK_LEIXX8.139e-4154.27S-adenosylmethionine synthetase OS=Leifsonia xyli ... [more]
METK_PSYA28.141e-4153.50S-adenosylmethionine synthetase OS=Psychrobacter a... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS04_0001L15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001L15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181955 ID=CN181955; Name=CN181955; organism=Citrus sinensis; type=EST; length=625bp
GAGGATTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCT
TAGATCTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACC
TTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTG
CGACCAGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTG
AGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTC
TTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCG
CGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACG
CCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATT
GCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGC
TGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAAT
TTATGCCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACG
GAGGTTAGGAAGAATAGTAACCTGCTCTTGGCTAAGGCCCGATGGGAAGA
CCCAGGTCACTGTTGAGTACTACAA
back to top