CN181955

Overview
NameCN181955
Unique NameCN181955
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length625
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig1234 contig Csv1_Contig1234:158..780. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_PICSI (S-adenosylmethionine synthetase 1 OS=Picea sitchensis GN=METK1 PE=2 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 1.117e-86
Identity = 143/158 (90.51%), Postives = 152/158 (96.20%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVM+FGEITTKA +DYEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGARLTEVRK+
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMIFGEITTKANIDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGARLTEVRKD 158

HSP 2 Score: 40.0466 bits (92), Expect = 1.117e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_VITVI (S-adenosylmethionine synthetase 3 OS=Vitis vinifera GN=METK3 PE=3 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 1.117e-86
Identity = 143/158 (90.51%), Postives = 153/158 (96.84%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETC+KTNMVMVFGEITTKAKVDYEKIVRDTCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH +K+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCSKTNMVMVFGEITTKAKVDYEKIVRDTCRGIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLSKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 1.117e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_ATRNU (S-adenosylmethionine synthetase 3 OS=Atriplex nummularia GN=SAMS3 PE=1 SV=1)

HSP 1 Score: 298.516 bits (763), Expect = 1.457e-86
Identity = 144/158 (91.14%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            ++TFLFTSESVNEGHPDKLCDQISDAVLDACL QDPESKVACETCTKTN+VMVFGEITTKA VDYEKIVR TCR IGFVS DVGLDAD+CKVLVNIEQQSPDIAQGVHGH T+RPE+IGAGDQGHMFGYATDET E MPLSHVLATKLGARLTEVRKN
Sbjct:    5 VDTFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPESKVACETCTKTNLVMVFGEITTKANVDYEKIVRQTCRDIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTRRPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKN 162

HSP 2 Score: 42.743 bits (99), Expect = 1.457e-86
Identity = 17/18 (94.44%), Postives = 17/18 (94.44%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEYY 623
            TC WLRPDGKTQVTVEYY
Sbjct:  164 TCPWLRPDGKTQVTVEYY 181
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK2_SOLTU (S-adenosylmethionine synthetase 2 OS=Solanum tuberosum GN=METK2 PE=2 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 1.457e-86
Identity = 143/158 (90.51%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFL TSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF+S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLLTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCREIGFISADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 1.457e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_ACTCH (S-adenosylmethionine synthetase 1 OS=Actinidia chinensis GN=SAM1 PE=2 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 1.457e-86
Identity = 142/159 (89.31%), Postives = 153/159 (96.23%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKNS 568
            M+TFLFTSESVNEGHPDKLCDQ+SDA+LDACL+QDPESKVACE+CTKTNMVMVFGEITTKA+V+YEKIVRDTCR IGF S DVGLDADHCKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKNS
Sbjct:    1 MDTFLFTSESVNEGHPDKLCDQVSDAILDACLKQDPESKVACESCTKTNMVMVFGEITTKAQVNYEKIVRDTCRGIGFTSPDVGLDADHCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNS 159

HSP 2 Score: 40.0466 bits (92), Expect = 1.457e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_CATRO (S-adenosylmethionine synthetase 3 OS=Catharanthus roseus GN=SAMS3 PE=1 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 2.480e-86
Identity = 143/158 (90.51%), Postives = 151/158 (95.57%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA V+YEKIVRDTCR IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVNYEKIVRDTCRGIGFTSPDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 2.480e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK4_BRAJU (S-adenosylmethionine synthetase 4 OS=Brassica juncea GN=MSAMS4 PE=2 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 3.235e-86
Identity = 143/158 (90.51%), Postives = 149/158 (94.30%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITT AKVDYEKIVR TCR IGF+S DVGLDAD C VLVNIEQQSPDIAQGVHGH TK+PEDIGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTSAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 3.235e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK3_ARATH (S-adenosylmethionine synthetase 3 OS=Arabidopsis thaliana GN=METK3 PE=1 SV=1)

HSP 1 Score: 300.056 bits (767), Expect = 3.235e-86
Identity = 144/158 (91.14%), Postives = 149/158 (94.30%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDA+LDACLEQDPESKVACETCTKTNMVMVFGEITT AKVDYEKIVR TCR IGF+S DVGLDAD C VLVNIEQQSPDIAQGVHGH TK+PEDIGAGDQGHMFGYATDET E MPL+HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 3.235e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK1_TOBAC (S-adenosylmethionine synthetase 1 OS=Nicotiana tabacum GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 299.671 bits (766), Expect = 4.219e-86
Identity = 143/158 (90.51%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQ+SDA+LDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGF S DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TK+PE+IGAGDQGHMFGYATDET E MPL+HV ATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQVSDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRGIGFTSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEIGAGDQGHMFGYATDETPELMPLTHVWATKLGAKLTEVRKN 158

HSP 2 Score: 40.0466 bits (92), Expect = 4.219e-86
Identity = 16/17 (94.12%), Postives = 16/17 (94.12%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRPDGKTQVTVEY
Sbjct:  160 TCPWLRPDGKTQVTVEY 176
[Close]
BLAST of CN181955 vs. ExPASy Swiss-Prot
Match: METK_PINBN (S-adenosylmethionine synthetase OS=Pinus banksiana GN=METK PE=2 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 5.504e-86
Identity = 145/158 (91.77%), Postives = 150/158 (94.94%), Query Frame = 2 
Query:   92 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETSEFMPLSHVLATKLGARLTEVRKN 565
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYE+IVR TCR IGF+SDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYATDET E MP  HVLATKLGA+LTEVRKN
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEQIVRKTCREIGFISDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYATDETLELMPKCHVLATKLGAKLTEVRKN 158

HSP 2 Score: 37.3502 bits (85), Expect = 5.504e-86
Identity = 15/17 (88.24%), Postives = 15/17 (88.24%), Query Frame = 3 
Query:  570 TCSWLRPDGKTQVTVEY 620
            TC WLRP GKTQVTVEY
Sbjct:  160 TCPWLRPHGKTQVTVEY 176
The following BLAST results are available for this feature:
BLAST of CN181955 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK1_PICSI1.117e-8690.51S-adenosylmethionine synthetase 1 OS=Picea sitchen... [more]
METK3_VITVI1.117e-8690.51S-adenosylmethionine synthetase 3 OS=Vitis vinifer... [more]
METK3_ATRNU1.457e-8691.14S-adenosylmethionine synthetase 3 OS=Atriplex numm... [more]
METK2_SOLTU1.457e-8690.51S-adenosylmethionine synthetase 2 OS=Solanum tuber... [more]
METK1_ACTCH1.457e-8689.31S-adenosylmethionine synthetase 1 OS=Actinidia chi... [more]
METK3_CATRO2.480e-8690.51S-adenosylmethionine synthetase 3 OS=Catharanthus ... [more]
METK4_BRAJU3.235e-8690.51S-adenosylmethionine synthetase 4 OS=Brassica junc... [more]
METK3_ARATH3.235e-8691.14S-adenosylmethionine synthetase 3 OS=Arabidopsis t... [more]
METK1_TOBAC4.219e-8690.51S-adenosylmethionine synthetase 1 OS=Nicotiana tab... [more]
METK_PINBN5.504e-8691.77S-adenosylmethionine synthetase OS=Pinus banksiana... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001L15_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001L15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181955 ID=CN181955; Name=CN181955; organism=Citrus sinensis; type=EST; length=625bp
GAGGATTGAGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCT
TAGATCTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACC
TTTCTATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTG
CGACCAGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTG
AGAGCAAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTC
TTTGGAGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCG
CGACACTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACG
CCGATCACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATT
GCCCAGGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGC
TGGTGACCAGGGCCATATGTTTGGTTATGCTACTGATGAGACCTCTGAAT
TTATGCCTTTGAGCCATGTCCTTGCCACCAAACTTGGTGCTCGTCTCACG
GAGGTTAGGAAGAATAGTAACCTGCTCTTGGCTAAGGCCCGATGGGAAGA
CCCAGGTCACTGTTGAGTACTACAA
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