CN181915 | Citrus Genome Database

Overview


Name	CN181915
Unique Name	CN181915
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	662

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.051e-20
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGG 76

HSP 2 Score: 82.8037 bits (203), Expect = 1.732e-15
Identity = 36/64 (56.25%), Postives = 49/64 (76.56%), Query Frame = -3

Query:  469 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 660
            ++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:   16 DIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 1.977e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -3

Query:  250 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 471
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76

HSP 2 Score: 81.6481 bits (200), Expect = 3.859e-15
Identity = 37/62 (59.68%), Postives = 49/62 (79.03%), Query Frame = -3

Query:  475 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 660
            ++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:   16 DIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.282e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 9.505e-14
Identity = 33/61 (54.10%), Postives = 47/61 (77.05%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            ++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   16 DIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.282e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 9.505e-14
Identity = 33/61 (54.10%), Postives = 47/61 (77.05%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            ++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   16 DIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.282e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 9.505e-14
Identity = 33/61 (54.10%), Postives = 47/61 (77.05%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            ++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   16 DIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.282e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 9.505e-14
Identity = 33/61 (54.10%), Postives = 47/61 (77.05%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            ++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   16 DIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.282e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 77.0258 bits (188), Expect = 9.505e-14
Identity = 33/61 (54.10%), Postives = 47/61 (77.05%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            ++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:   16 DIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.674e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 78.5666 bits (192), Expect = 3.267e-14
Identity = 34/61 (55.74%), Postives = 47/61 (77.05%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            ++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:   16 DIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.085e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 75.485 bits (184), Expect = 2.766e-13
Identity = 33/61 (54.10%), Postives = 46/61 (75.41%), Query Frame = -3

Query:  478 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 660
            E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:   16 EIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

BLAST of CN181915 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 2.417e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = -3

Query:  250 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 477
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76

HSP 2 Score: 73.559 bits (179), Expect = 1.051e-12
Identity = 31/62 (50.00%), Postives = 47/62 (75.81%), Query Frame = -3

Query:  475 EVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 660
            +++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:   16 DIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

The following BLAST results are available for this feature:

BLAST of CN181915 vs. ExPASy Swiss-Prot

BLAST of CN181915 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 98


Match Name	E-value	Identity	Description
NEDD8_DROME	3.051e-20	59.21	NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=...	[more]
RUB2_ARATH	1.977e-19	62.16	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	1.282e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	1.282e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	1.282e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	1.282e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	1.282e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	1.674e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	1.085e-17	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	2.417e-17	53.95	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS04_0001K18_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001K18, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN181915 ID=CN181915; Name=CN181915; organism=Citrus sinensis; type=EST; length=662bp
CCCCGGGCTGCAGGTTTTTTTTTTAGCATATATACACACACAAGACAATT
TATTCATGAATCAGAAAACCATAGCCTTATTAGAGGCCATTTCAGAGTAC
GACAACAGATAACAAAAGCTTATTATAAGCATACCATAAAACAACATTTT
GCAGCTTAATCTAAGTCATTAAGGCCACAAACGAAACAGAAAAAAGAAAG
CAAACCCAAAGAACACAGACACTGGCAGTGGACCAGAGTGCCATTAAAAA
CCACCACGCAGGCGAAGGACAAGATGTAAAGTAGACTCCTTCTGGATATT
GTAGTCAGCCAAAGTACGCCCATCCTCCAGCTGCTTCCCGGCAAAAATCA
ACCTCTGCTGATCTGGAGGAATCCCTTCCTTGTCCTGGATTTTAGCCTTC
ACATTATCAATGGTGTCTGAACTCTCAACCTCCAGGGTAATGGTTTTTCC
CGTCAATGTCTTCACGAAAATCTGCATTCCTCCACGCAGTCTCAACACCA
AATGCAGTGTGGATTCCTTCTGAATGTTGTAATCTGCAAGGGTCCTCCCA
TCTTCAAGCTGCTTGCCTGCAAAAATCAACCTCTGCTGGTCTGGTGGAAT
GCCCTCCTTATCTTGGATCTTGGCCTTCACATTATCAATAGTGTCCGAAC
TCTCTACCTCCA