CN181847

Overview
NameCN181847
Unique NameCN181847
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length696
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig242 contig Csv1_Contig242:166..860. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METTH (50S ribosomal protein L10e OS=Methanobacterium thermoautotrophicum GN=rpl10e PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.310e-19
Identity = 41/88 (46.59%), Postives = 56/88 (63.64%), Query Frame = -3 
Query:  302 RMLSILRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 565
            RM   L++RV+P H++R N M + AGADR+Q GMR AFGKP  T A V   Q ++++     N   A+EALRRA  KFP   +I++ R
Sbjct:   67 RMGYHLKIRVYPHHIVRENPMATGAGADRVQDGMRKAFGKPVSTVALVKKNQKIITIETNKKNFKDAKEALRRAAMKFPVPCRIVIDR 154

HSP 2 Score: 37.3502 bits (85), Expect = 2.310e-19
Identity = 17/42 (40.48%), Postives = 25/42 (59.52%), Query Frame = -2 
Query:  552 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFH 677
            EFP  + +       ++  ALEAARIA N+YM + AG+  +H
Sbjct:   30 EFPISLSVAVKAPTQITHNALEAARIASNRYMQRRAGRMGYH 71
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRCJ (50S ribosomal protein L10e OS=Pyrobaculum calidifontis (strain JCM 11548 / VA1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 2.989e-19
Identity = 46/84 (54.76%), Postives = 54/84 (64.29%), Query Frame = -3 
Query:  299 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRK 550
            LR+ V P HVLR N+ML+ AGADRLQ GMR AFG P G  ARV  GQV+  V  K  +  H +EALRRA  K P   +I+V  K
Sbjct:   88 LRLNVVPHHVLRENRMLAMAGADRLQEGMRLAFGSPAGRAARVEPGQVIFYVEFKPEHLAHVKEALRRAASKLPIPTRIVVEPK 171

HSP 2 Score: 32.3426 bits (72), Expect = 2.989e-19
Identity = 16/48 (33.33%), Postives = 24/48 (50.00%), Query Frame = -2 
Query:  531 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHFASEGAP 674
            F     LV+ E+  +  +ALEAAR   +KY+ KY G   ++      P
Sbjct:   47 FTMVAKLVAEERGQIRMQALEAARQISSKYLTKYVGDANYYLRLNVVP 94
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METJA (50S ribosomal protein L10e OS=Methanocaldococcus jannaschii GN=rpl10e PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 6.580e-19
Identity = 41/88 (46.59%), Postives = 55/88 (62.50%), Query Frame = -3 
Query:  302 RMLSILRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 565
            RM    ++RV+P  +LR +KM + AGADR+  GMR AFGKP GT ARV  GQ +L+V         A+EALRRA  K P   +I++ +
Sbjct:   80 RMGYKFQIRVYPHQILREHKMATGAGADRISDGMRLAFGKPIGTAARVKEGQAILTVWVNPDKFPAAKEALRRAAMKLPVPCRIVIEQ 167

HSP 2 Score: 40.4318 bits (93), Expect = 6.580e-19
Identity = 19/43 (44.19%), Postives = 26/43 (60.47%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            EFP  V+LV+     +   ALEAAR+A NKY+ K  G+  + F
Sbjct:   43 EFPVKVNLVATRPIQIRHNALEAARVAANKYLTKMCGRMGYKF 85
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRAR (50S ribosomal protein L10e OS=Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.110e-18
Identity = 43/84 (51.19%), Postives = 52/84 (61.90%), Query Frame = -3 
Query:  299 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRK 550
            LR+ V P HVLR N+ML+ AGADRLQ GMR AFG P G  ARV  GQ+L     K  +  H +EA RRA  K P   +I++  K
Sbjct:   88 LRLNVIPHHVLRENRMLAMAGADRLQEGMRLAFGSPAGRAARVEPGQILFYAEFKPEHLAHIKEAFRRAASKLPLPTRIVIEPK 171

HSP 2 Score: 33.8834 bits (76), Expect = 1.110e-18
Identity = 15/36 (41.67%), Postives = 21/36 (58.33%), Query Frame = -2 
Query:  567 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAG 674
            FP    L+  E+  +  +ALEAAR   +KY+ KY G
Sbjct:   47 FPMVAKLIVEERGQIRMQALEAARQMASKYLTKYVG 82
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_ARCFU (50S ribosomal protein L10e OS=Archaeoglobus fulgidus GN=rpl10e PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 1.113e-18
Identity = 41/83 (49.40%), Postives = 53/83 (63.86%), Query Frame = -3 
Query:  302 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 550
            L++R+ P H+LR +KM   AGADR+  GMR AFGKP G  ARV  G  ++SV  K  +   A+EALRRA  K P   KI+V +
Sbjct:   83 LKLRIFPHHILREHKMAVGAGADRISQGMRAAFGKPVGRAARVKPGTKIMSVWVKPEHFEIAKEALRRAAMKMPTPTKIVVEK 165

HSP 2 Score: 35.8094 bits (81), Expect = 1.113e-18
Identity = 16/37 (43.24%), Postives = 24/37 (64.86%), Query Frame = -2 
Query:  567 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAG 677
            +FP  + LV+ E   +   ALEAAR+  NKY+++ AG
Sbjct:   41 DFPVMLTLVAKEAVQIRENALEAARVVANKYVSRRAG 77
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRAE (50S ribosomal protein L10e OS=Pyrobaculum aerophilum GN=rpl10e PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.445e-18
Identity = 45/84 (53.57%), Postives = 53/84 (63.10%), Query Frame = -3 
Query:  299 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRK 550
            LR+ V P HVLR N+ML+ AGADRLQ GMR AFG P G  ARV  GQVL     K  +  H +EALRRA  K P   +I++  K
Sbjct:   88 LRLNVVPHHVLRENRMLAMAGADRLQEGMRLAFGSPAGRAARVEPGQVLFYAEFKPEHLPHIKEALRRAASKLPLPTRIVIEPK 171

HSP 2 Score: 31.5722 bits (70), Expect = 1.445e-18
Identity = 16/48 (33.33%), Postives = 23/48 (47.92%), Query Frame = -2 
Query:  531 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHFASEGAP 674
            F     LV  E+  +  +ALEAAR   +KY+ KY G   ++      P
Sbjct:   47 FTMTAKLVVEERGQIRMQALEAARQMASKYLTKYVGDANYYLRLNVVP 94
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_META3 (50S ribosomal protein L10e OS=Methanococcus aeolicus (strain Nankai-3 / ATCC BAA-1280) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 2.448e-18
Identity = 40/82 (48.78%), Postives = 54/82 (65.85%), Query Frame = -3 
Query:  302 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 547
            ++RV+P  VLR NKM S AGADR+  GMR +FGK  GT ARV  GQ ++++     N  HA+EALRR   K P + KI++ +
Sbjct:   85 QIRVYPHQVLRENKMASGAGADRISDGMRLSFGKAVGTAARVRKGQKIITISTTPENVIHAKEALRRCNMKMPVKCKIVIGK 166

HSP 2 Score: 33.113 bits (74), Expect = 2.448e-18
Identity = 17/43 (39.53%), Postives = 23/43 (53.49%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            +FP  VHLV  +   +   ALE+ARI  NKY     G+  + F
Sbjct:   42 DFPVEVHLVVKDDIQIRHNALESARIVGNKYTQNKCGRLGYKF 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRIL (50S ribosomal protein L10e OS=Pyrobaculum islandicum (strain DSM 4184 / JCM 9189) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 6.983e-18
Identity = 43/84 (51.19%), Postives = 53/84 (63.10%), Query Frame = -3 
Query:  299 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRK 550
            LR+ V P HVLR N+ML+ AGADRLQ GMR AFG P G  ARV  GQV+     +  +  H +EALRRA  K P   +I++  K
Sbjct:   88 LRLNVVPHHVLRENRMLAMAGADRLQEGMRLAFGSPAGRAARVEPGQVIFYAEFRPEHIAHIKEALRRAASKLPLPTRIVIETK 171

HSP 2 Score: 30.8018 bits (68), Expect = 6.983e-18
Identity = 16/48 (33.33%), Postives = 23/48 (47.92%), Query Frame = -2 
Query:  531 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHFASEGAP 674
            F     LV  E+  +  +ALEAAR    KY++KY G   ++      P
Sbjct:   47 FSMVAKLVVEERGQIRMQALEAARQMAYKYLSKYVGDANYYLRLNVVP 94
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_THEPD (50S ribosomal protein L10e OS=Thermofilum pendens (strain Hrk 5) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 7.011e-18
Identity = 45/83 (54.22%), Postives = 56/83 (67.47%), Query Frame = -3 
Query:  302 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 550
            LRV V P H+LR NKM++ AGADRLQ GMR +FG P G  ARV  GQ+++ V+    N  HA+EALRRA  K P   +IIV +
Sbjct:   84 LRVAVFPHHILRENKMMAMAGADRLQDGMRLSFGTPVGRAARVEAGQIVMLVKVDAKNIDHAKEALRRAASKIPLPSRIIVEQ 166

HSP 2 Score: 25.0238 bits (53), Expect = 7.011e-18
Identity = 14/40 (35.00%), Postives = 19/40 (47.50%), Query Frame = -2 
Query:  555 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAF 674
            F   + LV      + + ALEAAR   NKY+    G+  F
Sbjct:   43 FNTVLKLVVMRPGQIRANALEAARQHANKYLTAKVGEANF 82
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_STAMF (50S ribosomal protein L10e OS=Staphylothermus marinus (strain ATCC 43588 / DSM 3639 / F1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 9.162e-18
Identity = 48/96 (50.00%), Postives = 62/96 (64.58%), Query Frame = -3 
Query:  311 LATSTWPSTPARMLSILRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKII 598
            +A     ST       L++RV+P HVLR NKM++ AGADRLQ GMR AFGKP GT ARV  G +++ VR +  +  HA+EALRRA  K P   +I+
Sbjct:   68 MANKYLSSTVGDQNYFLKIRVYPHHVLRENKMMAFAGADRLQDGMRQAFGKPIGTAARVYPGTIVMEVRVRKEHVEHAKEALRRAASKLPLPARIV 163
The following BLAST results are available for this feature:
BLAST of CN181847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 88
Match NameE-valueIdentityDescription
RL10_METTH2.310e-1946.5950S ribosomal protein L10e OS=Methanobacterium the... [more]
RL10_PYRCJ2.989e-1954.7650S ribosomal protein L10e OS=Pyrobaculum calidifo... [more]
RL10_METJA6.580e-1946.5950S ribosomal protein L10e OS=Methanocaldococcus j... [more]
RL10_PYRAR1.110e-1851.1950S ribosomal protein L10e OS=Pyrobaculum arsenati... [more]
RL10_ARCFU1.113e-1849.4050S ribosomal protein L10e OS=Archaeoglobus fulgid... [more]
RL10_PYRAE1.445e-1853.5750S ribosomal protein L10e OS=Pyrobaculum aerophil... [more]
RL10_META32.448e-1848.7850S ribosomal protein L10e OS=Methanococcus aeolic... [more]
RL10_PYRIL6.983e-1851.1950S ribosomal protein L10e OS=Pyrobaculum islandic... [more]
RL10_THEPD7.011e-1854.2250S ribosomal protein L10e OS=Thermofilum pendens ... [more]
RL10_STAMF9.162e-1850.0050S ribosomal protein L10e OS=Staphylothermus mari... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS04_0001J04_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001J04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181847 ID=CN181847; Name=CN181847; organism=Citrus sinensis; type=EST; length=696bp
AAACAACTGCAAGGATCACATAGTTAAACCAAAGAAACATGTCACTTCAA
CTTGAGAAATCCAGTTCAACTGAGAAAAGGTACGAAATGGAAAATAATAA
ACTAGACAGTAGACCCAGCTCTGCTATTTTGAGACAGTAAATATCTTAGG
TTGTTGCTTCCAGGAAAGCTCTTCCAGGTTGACGTTGAGCCAAAGGACCA
TGACATCCGAGAAGCTTGGCATTCACACCATCTGGGACAATCCTGTTCTC
CGACTTCCACCGCAGATAATCAGCACGGCTGAACTTGGTAAATCCCCATT
TCCTGCTGACAATAATCTTTTGACGACCAGGAAACTTGAACTTAGCACGG
CGAAGGGCCTCCTGAGCATGGTGGCTGTTACTATCCTTGCAACGGACAGA
AAGCAAGACCTGCCCGATAGCAACACGAGCACAGGTTCCTTGGGGCTTTC
CAAAAGCACCCCTCATTCCAGTCTGGAGCCTATCAGCCCCAGCACACGAA
AGCATCTTGTTAATACGCAGAACATGGAAGGGGTGCACCCTCACTCGCAA
AATGGAAAGCATCCTTGCCGGCGTACTTGGCCATGTACTTGTTGCAAGCG
ATACGGGCAGCTTCCAAGGCCTCGCTCGACACGTTCTCCTTCTCCCAACT
GACGAGGTGAACGCAGAAGGGGAACTCGTCGACGCCTTTCTTCTTC
back to top