CN181847

Overview
NameCN181847
Unique NameCN181847
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length696
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METST (50S ribosomal protein L10e OS=Methanosphaera stadtmanae (strain DSM 3091) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 6.445e-15
Identity = 33/90 (36.67%), Postives = 53/90 (58.89%), Query Frame = -3 
Query:  281 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRKWGFTKF 550
            L++R +P H++R N M + AGADR+Q+GMRGAFGK   + A V   Q +++   +  +   A+ AL RA  K P   K+++ +     +F
Sbjct:   72 LKIRTYPHHIVRENPMATGAGADRVQSGMRGAFGKAVSSEALVRANQKIITAYVQVKDFEKAKVALNRAAMKLPVTCKLVIDKGHDLVQF 161

HSP 2 Score: 35.8094 bits (81), Expect = 6.445e-15
Identity = 18/38 (47.37%), Postives = 24/38 (63.16%), Query Frame = -2 
Query:  564 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGK 677
            EFP  V L   E  ++S  +LEAARIA N+Y+ +  GK
Sbjct:   30 EFPLEVTLAVKEHTHLSHNSLEAARIAANRYLQRTTGK 67
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METLZ (50S ribosomal protein L10e OS=Methanocorpusculum labreanum (strain ATCC 43576 / DSM 4855 / Z) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 8.366e-15
Identity = 35/81 (43.21%), Postives = 49/81 (60.49%), Query Frame = -3 
Query:  308 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIV 550
            L++R +P HVLR +K  + AGADR+  GMR AFGK  GT ARVA  Q + +V         A++AL  + +K P   ++IV
Sbjct:   84 LKIRAYPHHVLREHKQATGAGADRVSEGMRLAFGKAVGTAARVAPRQRVFTVWTTPQYVDQAKDALLHSGYKLPTPVRVIV 164

HSP 2 Score: 37.7354 bits (86), Expect = 8.366e-15
Identity = 17/50 (34.00%), Postives = 27/50 (54.00%), Query Frame = -2 
Query:  531 DEFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHFASEGAP 680
            ++FP  +HL + E   +   A+EAARI  N+ + K  G++ FH      P
Sbjct:   41 EDFPVAIHLEALEACQIRHTAMEAARINMNRRLMKTVGRNNFHLKIRAYP 90
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PICTO (50S ribosomal protein L10e OS=Picrophilus torridus GN=rpl10e PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.407e-14
Identity = 35/83 (42.17%), Postives = 56/83 (67.47%), Query Frame = -3 
Query:  302 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 550
            L++R +P  V+R +KM + AGADR+ +GMR AFG+P GT ARV  GQ+++  R   +N+   ++AL +A  K P   K++V++
Sbjct:   84 LQIRPYPHQVIREHKMATGAGADRISSGMRAAFGRPVGTAARVYPGQIIMVGRVDRNNAKLLKDALHKASIKLPTPCKVVVTK 166

HSP 2 Score: 26.9498 bits (58), Expect = 1.407e-14
Identity = 14/41 (34.15%), Postives = 23/41 (56.10%), Query Frame = -2 
Query:  555 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAF 677
            +F   + LV+ E   +   ALEAAR++ N+ +   AG D +
Sbjct:   42 DFEIEMMLVAEEACQIRHTALEAARVSVNRKLLDAAGSDNY 82
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METMA (50S ribosomal protein L10e OS=Methanosarcina mazei GN=rpl10e PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.379e-14
Identity = 36/81 (44.44%), Postives = 50/81 (61.73%), Query Frame = -3 
Query:  308 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIV 550
            +++RV+P  VLR NK  + AGADR+ +GMR AFGK  GT ARV   Q L +V  +  N   A++AL  A  K P   +I++
Sbjct:   85 MKLRVYPHEVLRENKQATGAGADRVSSGMRRAFGKNVGTAARVNSMQKLFTVAVEKQNFPAAKKALWHAGQKLPTPVRIVI 165

HSP 2 Score: 35.8094 bits (81), Expect = 2.379e-14
Identity = 18/41 (43.90%), Postives = 25/41 (60.98%), Query Frame = -2 
Query:  552 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFH 674
            FP  + LV+ EK  +   ALEAARI  N+++   AGK  F+
Sbjct:   44 FPVKISLVAEEKCQIRHTALEAARITANRHLVADAGKMGFY 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_HALMA (50S ribosomal protein L10e OS=Haloarcula marismortui GN=rpl10e PE=1 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 3.088e-14
Identity = 37/83 (44.58%), Postives = 47/83 (56.63%), Query Frame = -3 
Query:  302 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 550
            + +R  P  VLR NK  + AGADR+  GMR AFGK  GT ARV  G+ L +  C   ++ H +EA RRA  K     +I V R
Sbjct:   88 MTLRKFPHQVLRENKQATGAGADRVSDGMRAAFGKIVGTAARVQAGEQLFTAYCNVEDAEHVKEAFRRAYNKITPSCRIKVER 170

HSP 2 Score: 32.3426 bits (72), Expect = 3.088e-14
Identity = 13/45 (28.89%), Postives = 28/45 (62.22%), Query Frame = -2 
Query:  561 KKKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKD 695
            K+K  D++P  + L+  E   +   +LEA+R++ N+++ K  G++
Sbjct:   39 KQKDADDYPVQISLIVEETVQLRHGSLEASRLSANRHLIKELGEE 83
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_THESM (50S ribosomal protein L10e OS=Thermococcus sibiricus (strain MM 739 / DSM 12597) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 5.209e-14
Identity = 33/73 (45.21%), Postives = 43/73 (58.90%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +L+VR    +   A  A++RA  KFP
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKILTVRVNKQHLKFALAAMKRASMKFP 157

HSP 2 Score: 36.1946 bits (82), Expect = 5.209e-14
Identity = 17/43 (39.53%), Postives = 25/43 (58.14%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            +F F V L + E   +   ALEAAR   N+Y++K  G+  FH+
Sbjct:   42 DFEFEVSLHTAEPVQIRQNALEAARTQLNRYLSKNVGRSNFHY 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRFU (50S ribosomal protein L10e OS=Pyrococcus furiosus GN=rpl10e PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.142e-13
Identity = 34/73 (46.58%), Postives = 42/73 (57.53%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +LS+R    +   A E  RRA  KFP
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKILSIRVNRQHLKFAIEGARRAAMKFP 157

HSP 2 Score: 34.6538 bits (78), Expect = 1.142e-13
Identity = 17/43 (39.53%), Postives = 24/43 (55.81%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            +F F V L + E   +   ALEAAR   N+Y+ K  G+  +HF
Sbjct:   42 DFEFEVSLHTAEPVQIRQNALEAARQQVNRYLQKNVGRSNYHF 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METAC (50S ribosomal protein L10e OS=Methanosarcina acetivorans GN=rpl10e PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.145e-13
Identity = 36/81 (44.44%), Postives = 50/81 (61.73%), Query Frame = -3 
Query:  308 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIV 550
            +++RV+P  V+R NK  + AGADR+ +GMR AFGK  GT ARV   Q + +V  +  N   A+EAL  A  K P   +I+V
Sbjct:   85 MKLRVYPHEVIRENKQATGAGADRVSSGMRRAFGKNVGTAARVKPMQKIFTVAVEKQNFKAAKEALWHAGQKLPTPCRIVV 165

HSP 2 Score: 33.113 bits (74), Expect = 1.145e-13
Identity = 17/46 (36.96%), Postives = 24/46 (52.17%), Query Frame = -2 
Query:  552 KGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFH 689
            K    FP  + L+  EK  +   ALEAARI  N+++    GK  F+
Sbjct:   39 KANTSFPVKISLIVEEKCQIRHTALEAARITANRHLVADTGKMGFY 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METMJ (50S ribosomal protein L10e OS=Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.146e-13
Identity = 37/83 (44.58%), Postives = 48/83 (57.83%), Query Frame = -3 
Query:  302 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSR 550
            L++R  P HVLR NK  + AGADR+  GMR AFGK  GT ARV  GQ + SV         A+ +L+R  +K P   +I+  R
Sbjct:   84 LKIRTFPHHVLRENKQATGAGADRVSEGMRLAFGKAVGTAARVEKGQKVFSVWTSPQYVDKAKVSLKRGIYKLPTPARIVEER 166

HSP 2 Score: 30.4166 bits (67), Expect = 1.146e-13
Identity = 15/43 (34.88%), Postives = 24/43 (55.81%), Query Frame = -2 
Query:  552 DEFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFH 680
            +++P  + +V  E   +   ALEAARI  N+ + K  G+  FH
Sbjct:   41 EDYPVELSIVVDETCQIRHTALEAARIGINRQLQKEVGRANFH 83
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRHO (50S ribosomal protein L10e OS=Pyrococcus horikoshii GN=rpl10e PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.484e-13
Identity = 34/73 (46.58%), Postives = 42/73 (57.53%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +LS+R    +   A E  RRA  KFP
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKRDQKILSIRVNRQHLKFAIEGARRAAMKFP 157

HSP 2 Score: 34.2686 bits (77), Expect = 1.484e-13
Identity = 17/43 (39.53%), Postives = 24/43 (55.81%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            +F F V L + E   +   ALEAAR   N+Y+ K  G+  +HF
Sbjct:   42 DFEFEVALHTAEPVQIRQNALEAARQQVNRYLQKNVGRSNYHF 84
The following BLAST results are available for this feature:
BLAST of CN181847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 88
Match NameE-valueIdentityDescription
RL10_METST6.445e-1536.6750S ribosomal protein L10e OS=Methanosphaera stadt... [more]
RL10_METLZ8.366e-1543.2150S ribosomal protein L10e OS=Methanocorpusculum l... [more]
RL10_PICTO1.407e-1442.1750S ribosomal protein L10e OS=Picrophilus torridus... [more]
RL10_METMA2.379e-1444.4450S ribosomal protein L10e OS=Methanosarcina mazei... [more]
RL10_HALMA3.088e-1444.5850S ribosomal protein L10e OS=Haloarcula marismort... [more]
RL10_THESM5.209e-1445.2150S ribosomal protein L10e OS=Thermococcus sibiric... [more]
RL10_PYRFU1.142e-1346.5850S ribosomal protein L10e OS=Pyrococcus furiosus ... [more]
RL10_METAC1.145e-1344.4450S ribosomal protein L10e OS=Methanosarcina aceti... [more]
RL10_METMJ1.146e-1344.5850S ribosomal protein L10e OS=Methanoculleus maris... [more]
RL10_PYRHO1.484e-1346.5850S ribosomal protein L10e OS=Pyrococcus horikoshi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001J04_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001J04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181847 ID=CN181847; Name=CN181847; organism=Citrus sinensis; type=EST; length=696bp
AAACAACTGCAAGGATCACATAGTTAAACCAAAGAAACATGTCACTTCAA
CTTGAGAAATCCAGTTCAACTGAGAAAAGGTACGAAATGGAAAATAATAA
ACTAGACAGTAGACCCAGCTCTGCTATTTTGAGACAGTAAATATCTTAGG
TTGTTGCTTCCAGGAAAGCTCTTCCAGGTTGACGTTGAGCCAAAGGACCA
TGACATCCGAGAAGCTTGGCATTCACACCATCTGGGACAATCCTGTTCTC
CGACTTCCACCGCAGATAATCAGCACGGCTGAACTTGGTAAATCCCCATT
TCCTGCTGACAATAATCTTTTGACGACCAGGAAACTTGAACTTAGCACGG
CGAAGGGCCTCCTGAGCATGGTGGCTGTTACTATCCTTGCAACGGACAGA
AAGCAAGACCTGCCCGATAGCAACACGAGCACAGGTTCCTTGGGGCTTTC
CAAAAGCACCCCTCATTCCAGTCTGGAGCCTATCAGCCCCAGCACACGAA
AGCATCTTGTTAATACGCAGAACATGGAAGGGGTGCACCCTCACTCGCAA
AATGGAAAGCATCCTTGCCGGCGTACTTGGCCATGTACTTGTTGCAAGCG
ATACGGGCAGCTTCCAAGGCCTCGCTCGACACGTTCTCCTTCTCCCAACT
GACGAGGTGAACGCAGAAGGGGAACTCGTCGACGCCTTTCTTCTTC
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