CN181847

Overview
NameCN181847
Unique NameCN181847
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length696
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig242 contig Csv1_Contig242:166..860. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_THEON (50S ribosomal protein L10e OS=Thermococcus onnurineus (strain NA1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.927e-13
Identity = 34/73 (46.58%), Postives = 43/73 (58.90%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  ARV   Q +L+V   + +   A EA+RRA  K P
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVKKDQKILTVWVNEGHLKFALEAMRRAAMKLP 157

HSP 2 Score: 34.2686 bits (77), Expect = 1.927e-13
Identity = 16/43 (37.21%), Postives = 24/43 (55.81%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            EF + V L + +   +   ALEA RI  N+Y+ K  G+  +HF
Sbjct:   42 EFQYEVSLHAEQAMQIRQNALEAIRIQVNRYLQKNVGRSNYHF 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METS5 (50S ribosomal protein L10e OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 2.510e-13
Identity = 37/82 (45.12%), Postives = 55/82 (67.07%), Query Frame = -3 
Query:  308 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVA-IGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIV 550
            L V  +P HV+R NKM++ AGADRLQ GMR +FGKP GT AR+  +G +++++R K  +   A++A + A  K P   +I+V
Sbjct:   85 LYVTKYPHHVIRENKMMAFAGADRLQDGMRLSFGKPIGTAARITKLGDLIMAIRVKKEHLEFAKKAFKVASSKLPLDTEIVV 166

HSP 2 Score: 24.6386 bits (52), Expect = 2.510e-13
Identity = 12/39 (30.77%), Postives = 20/39 (51.28%), Query Frame = -2 
Query:  561 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKD 677
            ++ F V L++ +   +   ALEAAR+   K M    G +
Sbjct:   42 DYDFEVRLLTKQIGQIRHNALEAARVIALKQMTSMVGNE 80
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METHJ (50S ribosomal protein L10e OS=Methanospirillum hungatei (strain JF-1 / DSM 864) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 3.264e-13
Identity = 35/89 (39.33%), Postives = 49/89 (55.06%), Query Frame = -3 
Query:  299 RMLSILRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIVSRK 565
            RM   L++R +P HVLR NK  + AGADR+  GMR AFGK  GT AR    Q + +V    ++    ++ALR    K P    +++  K
Sbjct:   79 RMNYHLKLRTYPHHVLRENKQATGAGADRVSQGMRLAFGKAVGTAARCQQNQKIFTVFSNPASVEKIKDALRHGGHKLPSPTHLVIEMK 167

HSP 2 Score: 31.5722 bits (70), Expect = 3.264e-13
Identity = 17/42 (40.48%), Postives = 24/42 (57.14%), Query Frame = -2 
Query:  552 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFH 677
            EFP  V LV  E   +   ALEAAR++ N+ + K  G+  +H
Sbjct:   42 EFPMEVSLVVDESCQIRHSALEAARMSINRKLNKELGRMNYH 83
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_THEGJ (50S ribosomal protein L10e OS=Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 9.258e-13
Identity = 33/73 (45.21%), Postives = 42/73 (57.53%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  ARV   Q +L+V   + +   A  A+ RAK K P
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVKKDQKILTVWVNEQHLKFALGAMHRAKMKLP 157

HSP 2 Score: 34.2686 bits (77), Expect = 9.258e-13
Identity = 16/43 (37.21%), Postives = 24/43 (55.81%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            EF + V L + +   +   ALEA RI  N+Y+ K  G+  +HF
Sbjct:   42 EFEYEVSLHAEQAMQIRQNALEAIRIQVNRYLQKNVGRSNYHF 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRAB (50S ribosomal protein L10e OS=Pyrococcus abyssi GN=rpl10e PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 9.266e-13
Identity = 34/73 (46.58%), Postives = 42/73 (57.53%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  AR+   Q +LS+R    +   A E  RRA  KFP
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARLKKDQKVLSIRVNRQHLKFAIEGARRAAMKFP 157

HSP 2 Score: 31.9574 bits (71), Expect = 9.266e-13
Identity = 16/43 (37.21%), Postives = 23/43 (53.49%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            +F F V L   +   +   ALEAAR   N+Y+ K  G+  +HF
Sbjct:   42 DFEFEVALHVSQPVQIRQNALEAARQQVNRYLQKNVGRSNYHF 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_SULTO (50S ribosomal protein L10e OS=Sulfolobus tokodaii GN=rpl10e PE=3 SV=2)

HSP 1 Score: 66.6254 bits (161), Expect = 9.275e-13
Identity = 36/80 (45.00%), Postives = 51/80 (63.75%), Query Frame = -3 
Query:  314 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARV-AIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKI 550
            L V  +P HV+R NKM++ AGADRLQ GMR +FGKP GT AR+  +G +++  + K  +   A++A   A  K P + KI
Sbjct:   85 LIVLKYPHHVIRENKMMAFAGADRLQDGMRLSFGKPIGTAARIERLGDIIMIAKVKKEHLEIAKKAFEAAASKIPLKTKI 164

HSP 2 Score: 27.335 bits (59), Expect = 9.275e-13
Identity = 13/38 (34.21%), Postives = 20/38 (52.63%), Query Frame = -2 
Query:  561 FPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKD 674
            + + + LV+ EK  +   ALEAAR+   K +    G D
Sbjct:   43 YDYELRLVALEKGQIRHNALEAARVLALKQLTNKTGSD 80
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_PYRKO (50S ribosomal protein L10e OS=Pyrococcus kodakaraensis GN=rpl10e PE=3 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 2.029e-12
Identity = 33/73 (45.21%), Postives = 41/73 (56.16%), Query Frame = -3 
Query:  329 RVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFP 547
            ++RV+PF VLR N M +   ADR   GMR  FGKP G  ARV   Q +L+V     +   A  A+ RAK K P
Sbjct:   85 KIRVYPFQVLRENPMATGRKADRYGNGMRRPFGKPIGLAARVRKDQKILTVWVNGQHLKFALGAMHRAKMKLP 157

HSP 2 Score: 34.2686 bits (77), Expect = 2.029e-12
Identity = 16/43 (37.21%), Postives = 24/43 (55.81%), Query Frame = -2 
Query:  549 EFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFHF 677
            EF + V L + +   +   ALEA RI  N+Y+ K  G+  +HF
Sbjct:   42 EFEYEVSLHAEQAMQIRQNALEAIRIQVNRYLQKNVGRSNYHF 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_METBF (50S ribosomal protein L10e OS=Methanosarcina barkeri (strain Fusaro / DSM 804) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.035e-12
Identity = 35/81 (43.21%), Postives = 50/81 (61.73%), Query Frame = -3 
Query:  308 LRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARVAIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKIIV 550
            +++RV+P  VLR NK  + AGADR+ +GMR AFGK  GT ARV   Q + +V  +  N   A++AL  A  K P   +I++
Sbjct:   85 MKLRVYPHEVLRENKQATGAGADRVSSGMRRAFGKNVGTAARVNPFQKIFTVAVEKQNFVAAKKALWHAGQKLPTPCRIVI 165

HSP 2 Score: 31.187 bits (69), Expect = 2.035e-12
Identity = 16/46 (34.78%), Postives = 25/46 (54.35%), Query Frame = -2 
Query:  552 KGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMAKYAGKDAFH 689
            K    FP  + L+  EK  +   ALEAARI  N+++ +  GK  ++
Sbjct:   39 KANTSFPIKLSLLVEEKCQIRHTALEAARITANRHLIEDTGKMGYY 84
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_SULIY (50S ribosomal protein L10e OS=Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.752e-12
Identity = 38/75 (50.67%), Postives = 52/75 (69.33%), Query Frame = -3 
Query:  314 HPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARV-AIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKI 535
            +P HVLR NKM++ AGADRLQ GMR +FG P GT AR+  +G++L+ V+ K  +   A+EAL+ A  K P R +I
Sbjct:   90 YPHHVLRENKMMAFAGADRLQDGMRLSFGTPIGTAARIEKLGEILIVVKVKKEHLDFAKEALKIASKKLPLRTRI 164
[Close]
BLAST of CN181847 vs. ExPASy Swiss-Prot
Match: RL10_SULIN (50S ribosomal protein L10e OS=Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) GN=rpl10e PE=3 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.752e-12
Identity = 38/75 (50.67%), Postives = 52/75 (69.33%), Query Frame = -3 
Query:  314 HPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGTCARV-AIGQVLLSVRCKDSNSHHAQEALRRAKFKFPGRQKI 535
            +P HVLR NKM++ AGADRLQ GMR +FG P GT AR+  +G++L+ V+ K  +   A+EAL+ A  K P R +I
Sbjct:   90 YPHHVLRENKMMAFAGADRLQDGMRLSFGTPIGTAARIEKLGEILIVVKVKKEHLDFAKEALKIASKKLPLRTRI 164
The following BLAST results are available for this feature:
BLAST of CN181847 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 88
Match NameE-valueIdentityDescription
RL10_THEON1.927e-1346.5850S ribosomal protein L10e OS=Thermococcus onnurin... [more]
RL10_METS52.510e-1345.1250S ribosomal protein L10e OS=Metallosphaera sedul... [more]
RL10_METHJ3.264e-1339.3350S ribosomal protein L10e OS=Methanospirillum hun... [more]
RL10_THEGJ9.258e-1345.2150S ribosomal protein L10e OS=Thermococcus gammato... [more]
RL10_PYRAB9.266e-1346.5850S ribosomal protein L10e OS=Pyrococcus abyssi GN... [more]
RL10_SULTO9.275e-1345.0050S ribosomal protein L10e OS=Sulfolobus tokodaii ... [more]
RL10_PYRKO2.029e-1245.2150S ribosomal protein L10e OS=Pyrococcus kodakarae... [more]
RL10_METBF2.035e-1243.2150S ribosomal protein L10e OS=Methanosarcina barke... [more]
RL10_SULIY5.752e-1250.6750S ribosomal protein L10e OS=Sulfolobus islandicu... [more]
RL10_SULIN5.752e-1250.6750S ribosomal protein L10e OS=Sulfolobus islandicu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001J04_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001J04, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181847 ID=CN181847; Name=CN181847; organism=Citrus sinensis; type=EST; length=696bp
AAACAACTGCAAGGATCACATAGTTAAACCAAAGAAACATGTCACTTCAA
CTTGAGAAATCCAGTTCAACTGAGAAAAGGTACGAAATGGAAAATAATAA
ACTAGACAGTAGACCCAGCTCTGCTATTTTGAGACAGTAAATATCTTAGG
TTGTTGCTTCCAGGAAAGCTCTTCCAGGTTGACGTTGAGCCAAAGGACCA
TGACATCCGAGAAGCTTGGCATTCACACCATCTGGGACAATCCTGTTCTC
CGACTTCCACCGCAGATAATCAGCACGGCTGAACTTGGTAAATCCCCATT
TCCTGCTGACAATAATCTTTTGACGACCAGGAAACTTGAACTTAGCACGG
CGAAGGGCCTCCTGAGCATGGTGGCTGTTACTATCCTTGCAACGGACAGA
AAGCAAGACCTGCCCGATAGCAACACGAGCACAGGTTCCTTGGGGCTTTC
CAAAAGCACCCCTCATTCCAGTCTGGAGCCTATCAGCCCCAGCACACGAA
AGCATCTTGTTAATACGCAGAACATGGAAGGGGTGCACCCTCACTCGCAA
AATGGAAAGCATCCTTGCCGGCGTACTTGGCCATGTACTTGTTGCAAGCG
ATACGGGCAGCTTCCAAGGCCTCGCTCGACACGTTCTCCTTCTCCCAACT
GACGAGGTGAACGCAGAAGGGGAACTCGTCGACGCCTTTCTTCTTC
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