CN181794

Overview
NameCN181794
Unique NameCN181794
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length668
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4B_ARATH (26S proteasome regulatory subunit 4 homolog B OS=Arabidopsis thaliana GN=RPT2B PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.673e-15
Identity = 52/178 (29.21%), Postives = 82/178 (46.07%), Query Frame = 2 
Query:   89 KQPESAAPEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRI 622
            K PE+AA           K    LR   L+ I +  L   +   N  RL+ Q  +        + ++  L+     VG + +++ +N  +V      +Y V            P   + + N    +  IL  +VDP+V++MKVEK P  +Y  IGGL+ QI+EIKE +ELP+ HP +
Sbjct:   44 KGPEAAARLPTVTPSTKCK----LRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEED----RSKVDDLRGTPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPEL 213

HSP 2 Score: 30.0314 bits (66), Expect = 1.673e-15
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PEL+E +GI  P+GV+LY
Sbjct:  211 PELYEDIGIKPPKGVILY 228
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4A_ARATH (26S proteasome regulatory subunit 4 homolog A OS=Arabidopsis thaliana GN=RPT2A PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.673e-15
Identity = 52/178 (29.21%), Postives = 82/178 (46.07%), Query Frame = 2 
Query:   89 KQPESAAPEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRI 622
            K PE+AA           K    LR   L+ I +  L   +   N  RL+ Q  +        + ++  L+     VG + +++ +N  +V      +Y V            P   + + N    +  IL  +VDP+V++MKVEK P  +Y  IGGL+ QI+EIKE +ELP+ HP +
Sbjct:   44 KGPEAAARLPTVTPSTKCK----LRLLKLERIKDYLLMEEEFVANQERLKPQEEKAEED----RSKVDDLRGTPMSVGNLEELIDENHAIVSSSVGPEYYVGILSFVDKDQLEPGCSILMHNKVLSVVGILQDEVDPMVSVMKVEKAPLESYADIGGLEAQIQEIKEAVELPLTHPEL 213

HSP 2 Score: 30.0314 bits (66), Expect = 1.673e-15
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PEL+E +GI  P+GV+LY
Sbjct:  211 PELYEDIGIKPPKGVILY 228
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4_DICDI (26S protease regulatory subunit 4 homolog OS=Dictyostelium discoideum GN=psmC1 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.671e-15
Identity = 44/155 (28.39%), Postives = 81/155 (52.26%), Query Frame = 2 
Query:  158 LRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRI 622
            L+Q  L+ I +  L  ++   N +  + + +E NS+ +  ++ ++ L+     VG + +++  N  +V      ++ V                V L N +  +  ++  +VDP+VN+MKVEK P  +Y  IGGL+ Q++E+KE IELP+ HP +
Sbjct:   56 LKQLKLERIKDYLLMEQEFLQNYDLNQPKVDE-NSKEQADEKIIEELRGDPLTVGNLEEIIDDNHAIVSSTVGPEHYVRIMSFVDKSKLYLGATVLLNNKTLSVVGVIDGEVDPMVNVMKVEKAPTESYSDIGGLEAQVQEMKEAIELPLTHPEL 209

HSP 2 Score: 30.0314 bits (66), Expect = 3.671e-15
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PEL+E +GI  P+GV+LY
Sbjct:  207 PELYEEIGIKPPKGVILY 224
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PSMR_AERPE (Proteasome-activating nucleotidase OS=Aeropyrum pernix GN=pan PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.779e-15
Identity = 44/147 (29.93%), Postives = 71/147 (48.30%), Query Frame = 2 
Query:  188 ELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRIVR 628
            E  +++R     +  L  ++  L   +   K E+  L  P      V++V+  N+V+VK       +V            P   VAL N    +  +LP + DPLV  M+VE+ P   +  +GGL++QI+EI E + LPIK+P + R
Sbjct:   20 ERDVEIRILKDKVRSLTKEKISLQKELEYYKNEITKLLSPPYIEAVVLEVIDDNRVVVKSSTGPNLIVNVAAGVDARSLKPGAIVALNNRGSTIVDVLPGRYDPLVKAMEVEERPKVFFKDVGGLEEQIREIYEAVVLPIKNPHLFR 166

HSP 2 Score: 26.5646 bits (57), Expect = 4.779e-15
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            P LF  LGI  P+GVLL+
Sbjct:  162 PHLFRELGIDPPKGVLLH 179
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PSMR_METMA (Proteasome-activating nucleotidase OS=Methanosarcina mazei GN=pan PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.206e-15
Identity = 42/148 (28.38%), Postives = 76/148 (51.35%), Query Frame = 2 
Query:  191 LQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP-RIVRV 631
            L+ Q  Q       LE Q+ +    +R L+ EL  ++     +G V+ V+  ++++V+     +++V            P  +VAL   +  +  ++PS  +P V  M+V +  +  YD IGGLD+QI+E++E +ELP+  P R  R+
Sbjct:   41 LEEQCSQIESEKRYLENQKIKYEREIRKLQSELDRMKTSPLIIGTVIDVIKNDRIIVRSSNGPQFLVNVSQYIDEKKLLPGAKVALNQHTLAIAEVIPSTEEPFVAAMEVIESVEVDYDQIGGLDEQIQELQEAVELPLTSPERFARI 188

HSP 2 Score: 26.9498 bits (58), Expect = 6.206e-15
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F  +GI  P+GVLLY
Sbjct:  182 PERFARIGIEPPKGVLLY 199
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4_CAEEL (Probable 26S protease regulatory subunit 4 OS=Caenorhabditis elegans GN=rpt-2 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 8.054e-15
Identity = 45/157 (28.66%), Postives = 75/157 (47.77%), Query Frame = 2 
Query:  146 QGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            + E ++ Y L     +Q Q R K     R E +R +++            L+     VG + +++     +V  +   ++ V            P   V L + ++ +  +L    DP+V++MK+EK P  TY  +GGLDQQI+EIKE +ELP+ HP
Sbjct:   67 KSERIKDYLLMEQEFIQNQERLKPQE-ERQEEERAKVDE-----------LRGTPMAVGSLEEIIDDQHAIVSTNVGSEHYVNIMSFVDKEQLEPGCSVLLNHKNHAVIGVLSDDTDPMVSVMKLEKAPQETYADVGGLDQQIQEIKEAVELPLTHP 211

HSP 2 Score: 28.1054 bits (61), Expect = 8.054e-15
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE +E +GI  P+GV+LY
Sbjct:  211 PEYYEEMGIRPPKGVILY 228
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS7B_ARATH (26S protease regulatory subunit 7 homolog B OS=Arabidopsis thaliana GN=RPT1B PE=3 SV=2)

HSP 1 Score: 75.0998 bits (183), Expect = 1.045e-14
Identity = 45/126 (35.71%), Postives = 67/126 (53.17%), Query Frame = 2 
Query:  251 ELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKN----KVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            +L S  +M++EE  LL      V    +++  N    K +V +   GKYVV               RV +    Y + + LP K+DP V +M VE+ PD+TY  IGG  +QI++I+EV+ELP+ HP
Sbjct:  109 DLVSDKQMMQEEQPLL------VATCTQIISPNTEDAKYVVDIKKIGKYVVGLGDKASPTDIEAGMRVGVDQKKYQIQIPLPPKIDPSVTMMTVEEKPDATYSDIGGCKEQIEKIREVVELPMLHP 228

HSP 2 Score: 25.0238 bits (53), Expect = 1.045e-14
Identity = 11/18 (61.11%), Postives = 12/18 (66.67%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F  LGI  P+GVL Y
Sbjct:  228 PEKFVRLGIDPPKGVLCY 245
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PSMR_METAC (Proteasome-activating nucleotidase OS=Methanosarcina acetivorans GN=pan PE=3 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 1.048e-14
Identity = 42/148 (28.38%), Postives = 76/148 (51.35%), Query Frame = 2 
Query:  191 LQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP-RIVRV 631
            L+ Q  Q       LE Q+ +    +R L+ EL  ++     +G V+ V+  ++++V+     +++V            P  +VAL   +  +  ++PS  +P V  M+V +  +  YD IGGLD+QI+E++E +ELP+  P R  R+
Sbjct:   41 LEEQCSQIESEKRYLENQKIKYEREIRKLQSELDRMKTSPLIIGTVIDVIKNDRIIVRSSNGPQFLVNVSQYIDEKKLLPGAKVALNQHTLAIAEVIPSTEEPFVAAMEVIESIEVDYDQIGGLDEQIQELQEAVELPLIEPERFARI 188

HSP 2 Score: 26.9498 bits (58), Expect = 1.048e-14
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F  +GI  P+GVLLY
Sbjct:  182 PERFARIGIEPPKGVLLY 199
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4_SCHPO (26S protease regulatory subunit 4 homolog OS=Schizosaccharomyces pombe GN=mts2 PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.359e-14
Identity = 45/156 (28.85%), Postives = 76/156 (48.72%), Query Frame = 2 
Query:  158 LRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVH-PEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRI 622
            LR   +Q IH+  L   +   N  RL+ Q    + R +  +  +  ++     VG + +++  +  +V    PE  Y V            P   V L + +  +  +L    DP++N+MK++K P  +Y  IGGL+ QI+EIKE +ELP+ HP +
Sbjct:   67 LRLLKMQRIHDHLLMEEEYVQNQERLKPQ----DERTQEERNRVDEIRGTPMSVGTLEEIIDDDHAIVSTAGPE--YYVSIMSFVDKDMLEPGCSVLLHHKAMSIVGLLLDDTDPMINVMKLDKAPTESYADIGGLESQIQEIKEAVELPLTHPEL 216

HSP 2 Score: 30.0314 bits (66), Expect = 1.359e-14
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PEL+E +GI  P+GV+LY
Sbjct:  214 PELYEEMGIKPPKGVILY 231
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4_RAT (26S protease regulatory subunit 4 OS=Rattus norvegicus GN=Psmc1 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 1.360e-14
Identity = 57/184 (30.98%), Postives = 85/184 (46.20%), Query Frame = 2 
Query:   83 ETKQPESAA------PEEVCAAKGASKQGEGLRQYYLQHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            +TK P++A+      P   C  K      E ++ Y L     ++ Q + K      LE ++ E  S+V  L+        P S VG + +++  N  +V      ++ V            P   V L +  + +  +L    DPLV +MKVEK P  TY  IGGLD QI+EIKE +ELP+ HP
Sbjct:   39 KTKGPDAASKLPLVTPHTQCRLKLLKL--ERIKDYLLMEEEFIRNQEQMKP-----LEEKQEEERSKVDDLRGT------PMS-VGTLEEIIDDNHAIVSTSVGSEHYVSILSFVDKDLLEPGCSVLLNHKVHAVIGVLMDDTDPLVTVMKVEKAPQETYADIGGLDNQIQEIKESVELPLTHP 208

HSP 2 Score: 28.1054 bits (61), Expect = 1.360e-14
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE +E +GI  P+GV+LY
Sbjct:  208 PEYYEEMGIKPPKGVILY 225
The following BLAST results are available for this feature:
BLAST of CN181794 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
PRS4B_ARATH1.673e-1529.2126S proteasome regulatory subunit 4 homolog B OS=A... [more]
PRS4A_ARATH1.673e-1529.2126S proteasome regulatory subunit 4 homolog A OS=A... [more]
PRS4_DICDI3.671e-1528.3926S protease regulatory subunit 4 homolog OS=Dicty... [more]
PSMR_AERPE4.779e-1529.93Proteasome-activating nucleotidase OS=Aeropyrum pe... [more]
PSMR_METMA6.206e-1528.38Proteasome-activating nucleotidase OS=Methanosarci... [more]
PRS4_CAEEL8.054e-1528.66Probable 26S protease regulatory subunit 4 OS=Caen... [more]
PRS7B_ARATH1.045e-1435.7126S protease regulatory subunit 7 homolog B OS=Ara... [more]
PSMR_METAC1.048e-1428.38Proteasome-activating nucleotidase OS=Methanosarci... [more]
PRS4_SCHPO1.359e-1428.8526S protease regulatory subunit 4 homolog OS=Schiz... [more]
PRS4_RAT1.360e-1430.9826S protease regulatory subunit 4 OS=Rattus norveg... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001H20_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001H20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181794 ID=CN181794; Name=CN181794; organism=Citrus sinensis; type=EST; length=668bp
GAGGCGAGACTTGATCCGAAAGACAATTAGATTCGAACAAATCGAACTCG
TTCACTCGGAAAGAATGGCCGCTGTAGGAATCGAAACGAAGCAGCCGGAA
TCGGCGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGCATCGAAACAAGG
AGAGGGATTGAGGCAATACTACCTCCAACACATTCACGAACTCCAGCTCC
AAGTCCGTCAAAAAACCCACAATCTCAATCGCCTCGAAGCCCAGCGTAAC
GAACTCAATTCTAGAGTGCGGATGCTTAAAGAAGAATTACAGCTTCTTCA
AGAGCCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTATGGGAAAAAATA
AGGTTCTTGTTAAGGTTCATCCTGAGGGGAAATATGTTGTTGATATTGAT
AAAAGTATTGATATAACCAAGATCACTCCGTCAACAAGAGTTGCACTTCG
TAATGACAGCTATGTTCTTCATTTGATCCTGCCAAGCAAAGTAGATCCCC
TGGTCAACCTTATGAAGGTTGAAAAGGTCCCTGATTCTACATATGACATG
ATTGGTGGCCTTGATCAGCAAATTAAGGAGATCAAGGAGGTCATTGAGCT
TCCTATCAAACATCCCCGAATTGTTCGAGTCTCTTGGAATAGCTCAACCC
AGGGGTGTATTGCTATAT
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