CN181794

Overview
NameCN181794
Unique NameCN181794
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length668
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_MANSE (26S protease regulatory subunit 6B OS=Manduca sexta PE=2 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.307e-13
Identity = 42/154 (27.27%), Postives = 72/154 (46.75%), Query Frame = 2 
Query:  188 ELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRIVR 628
            +L  + ++    L  LE Q   +    R LK       EE++ +Q     +G+ ++ + +N  +V       Y V            PS  VAL   S  L  +LP + D  +++++ ++ PD  Y  IGG+D Q +EI+E +ELP+ H  + R
Sbjct:   36 DLYTKYKKLQRMLEFLEVQEEYIKDEQRNLKKEYLHAQEEVKRIQSVPLVIGQFLEAVDQNTGIVGSTTGSNYYVRILSTIDRELLKPSASVALHKHSNALVDVLPPEADSSISMLQADEKPDVQYSDIGGMDTQKQEIREAVELPLTHVELYR 189

HSP 2 Score: 26.5646 bits (57), Expect = 5.307e-13
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL+  +GI  PRGVL+Y
Sbjct:  186 ELYRQIGIEPPRGVLMY 202
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS7_DICDI (26S protease regulatory subunit 7 OS=Dictyostelium discoideum GN=psmC2 PE=1 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.887e-13
Identity = 43/158 (27.22%), Postives = 78/158 (49.37%), Query Frame = 2 
Query:  170 YLQHIHELQLQVRQKTHNLNRLEAQRNELN-----SRVRMLKEELQLLQEPGSYVGEVVKVM--GKN--KVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            Y + I  L+  +++ T  +N L   +         S+  ++ ++    +EP   V    K++  GK   K ++ V    K+VV               RV +  + Y + + LP K+D  V +M+VE+ PD TY  +GG  +QI++++EV+E+P+ HP
Sbjct:   36 YSKSIRILEEDIKKMTSKVNELCGIKESDTGIGPPSQWDLVVDKTSAHEEPPLQVARCTKIIDVGKPNAKYIITVKQIAKFVVALGDKLSPTDVEEGIRVGVDRNKYQIQIPLPPKIDASVTMMQVEEKPDITYKDVGGCKEQIEKLREVVEMPLLHP 193

HSP 2 Score: 26.9498 bits (58), Expect = 6.887e-13
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F +LGI  P+GVL+Y
Sbjct:  193 PEKFVNLGIDPPKGVLMY 210
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PSMR1_HALSA (Proteasome-activating nucleotidase 1 OS=Halobacterium salinarium GN=pan1 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 8.954e-13
Identity = 39/131 (29.77%), Postives = 66/131 (50.38%), Query Frame = 2 
Query:  224 LNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            L+ +EA+R EL   V  L+ E + L+    Y+  V  +      ++K H   + V+               RVA+ NDS+ +  +L  + D     M+V++ P  TY  IGGLD Q++E++E +E P+ +P
Sbjct:   46 LDDVEARREELREEVNRLQRENETLKTASLYLATVEDLPEDGSAVIKQHGNNQEVLTELSPRLADTLEVGDRVAI-NDSFSVQRVLDDETDARAQAMEVDESPSVTYADIGGLDDQLREVREAVEDPLVNP 175

HSP 2 Score: 25.0238 bits (53), Expect = 8.954e-13
Identity = 9/18 (50.00%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F+++G+  P GVLL+
Sbjct:  175 PEKFDAVGVEPPSGVLLH 192
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4_YEAST (26S protease regulatory subunit 4 homolog OS=Saccharomyces cerevisiae GN=RPT2 PE=1 SV=3)

HSP 1 Score: 62.7734 bits (151), Expect = 1.508e-12
Identity = 33/115 (28.70%), Postives = 60/115 (52.17%), Query Frame = 2 
Query:  278 KEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRI 622
            K++L+ ++     +G + +++  +  +V       Y V            P   V L + +  +  +L    DP+V++MK++K P  +Y  IGGL+ QI+EIKE +ELP+ HP +
Sbjct:   93 KKQLEEIRGNPLSIGTLEEIIDDDHAIVTSPTMPDYYVSILSFVDKELLEPGCSVLLHHKTMSIVGVLQDDADPMVSVMKMDKSPTESYSDIGGLESQIQEIKESVELPLTHPEL 207

HSP 2 Score: 30.0314 bits (66), Expect = 1.508e-12
Identity = 11/18 (61.11%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PEL+E +GI  P+GV+LY
Sbjct:  205 PELYEEMGIKPPKGVILY 222
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_ASPNG (26S protease regulatory subunit 6B homolog OS=Aspergillus niger GN=tbpA PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.959e-12
Identity = 40/144 (27.78%), Postives = 71/144 (49.31%), Query Frame = 2 
Query:  176 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 607
            +H+  +QLQ          ++ ++  L   +   +EE++ +Q     +G+ ++ + +N  +V+      YVV            PS+ VAL   S  L  ILP + D  + ++   + PD TY  +GGLD Q +EI+E +ELP+
Sbjct:   50 RHLEYIQLQEEY-------IKDEQRSLKRELVRAQEEIKRIQSVPLVIGQFMEAIDQNTGIVQSSTGSNYVVRILSTLDREKLKPSSSVALHRHSNALVDILPPEADSSIAMLGENEKPDVTYADVGGLDMQKQEIREAVELPL 186

HSP 2 Score: 26.1794 bits (56), Expect = 1.959e-12
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            +L++ +GI  PRGVLLY
Sbjct:  190 DLYKQIGIDPPRGVLLY 206
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS7_SCHPO (26S protease regulatory subunit 7 homolog OS=Schizosaccharomyces pombe GN=rpt1 PE=1 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 2.542e-12
Identity = 32/91 (35.16%), Postives = 49/91 (53.85%), Query Frame = 2 
Query:  344 KNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            KN  ++ +    K+VV               RV    + Y + L LP K+DP V +M+VE+ PD TY  +GG  +QI+ ++EV+ELP+  P
Sbjct:  112 KNAYVINLKQIAKFVVSLGERVSPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTMMQVEEKPDVTYGDVGGCKEQIERLREVVELPLLSP 202

HSP 2 Score: 26.1794 bits (56), Expect = 2.542e-12
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F  LGI  P+G++LY
Sbjct:  202 PERFVKLGIDPPKGIMLY 219
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_CAEEL (Probable 26S protease regulatory subunit 6B OS=Caenorhabditis elegans GN=rpt-3 PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 3.306e-12
Identity = 40/149 (26.85%), Postives = 71/149 (47.65%), Query Frame = 2 
Query:  173 LQHIHELQLQVRQKTHNLNR--LEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKH 613
            L H+  ++  ++ +T NL +  L AQ            EE++ +Q     +G+ ++ + +N  +V       Y V            P   VAL   S  L  +LP + D  + +++ ++ PD +Y  IGGLD Q +E++E +ELP+ H
Sbjct:   47 LAHLQVMEDYIKLETRNLEKELLHAQ------------EEVKRIQSVPLVIGQFLEAVDQNHAIVGSTTGSNYYVRVLSILDRELLKPGCSVALHKYSNALVDVLPPEADSSIQMLRPDEKPDISYGDIGGLDMQKQEVREAVELPLTH 183

HSP 2 Score: 26.9498 bits (58), Expect = 3.306e-12
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVL+Y
Sbjct:  185 ELYQQIGIDPPRGVLMY 201
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_SOLTU (26S protease regulatory subunit 6B homolog OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.307e-12
Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 2 
Query:  188 ELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRIVR 628
            +L  +++     L  +E Q   +   ++ L+       EE++ +Q     +G+ ++++ +N  +V       Y V            PS  V L   S  L  +LP + D  ++L+   + PD TY+ IGG D Q +EI+E +ELP+ H  + +
Sbjct:   34 DLYARLKSLQRQLEFIEIQEEYVKDELKNLRREHLRAQEEVKRIQSVPLVIGQFMEMIDQNNAIVGSTTGSNYYVRILSTINRELLKPSASVGLDRHSNALVDVLPPEADSSISLLSQSEKPDVTYNDIGGCDIQKQEIREAVELPLTHHELYK 187

HSP 2 Score: 27.335 bits (59), Expect = 3.307e-12
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVLLY
Sbjct:  184 ELYKQIGIDPPRGVLLY 200
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_YEAST (26S protease regulatory subunit 6B homolog OS=Saccharomyces cerevisiae GN=RPT3 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.289e-12
Identity = 39/142 (27.46%), Postives = 70/142 (49.30%), Query Frame = 2 
Query:  182 IHELQLQVRQKTHNLNRLEAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPI 607
            + +L+ +    T   + ++ ++  L   ++  +EE++ +Q     +G+ ++ + +N  +V       YVV            PS  VAL   S  L  ILP   D  +++M   + PD TY  +GGLD Q +EI+E +ELP+
Sbjct:   51 LKKLEKEYELLTLQEDYIKDEQRHLKRELKRAQEEVKRIQSVPLVIGQFLEPIDQNTGIVSSTTGMSYVVRILSTLDRELLKPSMSVALHRHSNALVDILPPDSDSSISVMGENEKPDVTYADVGGLDMQKQEIREAVELPL 192

HSP 2 Score: 27.7202 bits (60), Expect = 4.289e-12
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            +L+E +GI  PRGVLLY
Sbjct:  196 DLYEQIGIDPPRGVLLY 212
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS4_ENCCU (26S protease regulatory subunit 4 homolog OS=Encephalitozoon cuniculi GN=RPT2 PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 4.290e-12
Identity = 26/49 (53.06%), Postives = 39/49 (79.59%), Query Frame = 2 
Query:  476 ILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRI 622
            +L  ++DP+VN+MK+EK P  TY  IGGL++QI+EIKE +ELP+ +P +
Sbjct:  146 VLEGEMDPMVNVMKLEKAPSETYADIGGLEEQIQEIKESVELPLTNPEL 194

HSP 2 Score: 28.8758 bits (63), Expect = 4.290e-12
Identity = 10/18 (55.56%), Postives = 15/18 (83.33%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PEL++ +GI  P+GV+LY
Sbjct:  192 PELYQEMGIKPPKGVILY 209
The following BLAST results are available for this feature:
BLAST of CN181794 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
PRS6B_MANSE5.307e-1327.2726S protease regulatory subunit 6B OS=Manduca sext... [more]
PRS7_DICDI6.887e-1327.2226S protease regulatory subunit 7 OS=Dictyostelium... [more]
PSMR1_HALSA8.954e-1329.77Proteasome-activating nucleotidase 1 OS=Halobacter... [more]
PRS4_YEAST1.508e-1228.7026S protease regulatory subunit 4 homolog OS=Sacch... [more]
PRS6B_ASPNG1.959e-1227.7826S protease regulatory subunit 6B homolog OS=Aspe... [more]
PRS7_SCHPO2.542e-1235.1626S protease regulatory subunit 7 homolog OS=Schiz... [more]
PRS6B_CAEEL3.306e-1226.85Probable 26S protease regulatory subunit 6B OS=Cae... [more]
PRS6B_SOLTU3.307e-1225.3226S protease regulatory subunit 6B homolog OS=Sola... [more]
PRS6B_YEAST4.289e-1227.4626S protease regulatory subunit 6B homolog OS=Sacc... [more]
PRS4_ENCCU4.290e-1253.0626S protease regulatory subunit 4 homolog OS=Encep... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001H20_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001H20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181794 ID=CN181794; Name=CN181794; organism=Citrus sinensis; type=EST; length=668bp
GAGGCGAGACTTGATCCGAAAGACAATTAGATTCGAACAAATCGAACTCG
TTCACTCGGAAAGAATGGCCGCTGTAGGAATCGAAACGAAGCAGCCGGAA
TCGGCGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGCATCGAAACAAGG
AGAGGGATTGAGGCAATACTACCTCCAACACATTCACGAACTCCAGCTCC
AAGTCCGTCAAAAAACCCACAATCTCAATCGCCTCGAAGCCCAGCGTAAC
GAACTCAATTCTAGAGTGCGGATGCTTAAAGAAGAATTACAGCTTCTTCA
AGAGCCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTATGGGAAAAAATA
AGGTTCTTGTTAAGGTTCATCCTGAGGGGAAATATGTTGTTGATATTGAT
AAAAGTATTGATATAACCAAGATCACTCCGTCAACAAGAGTTGCACTTCG
TAATGACAGCTATGTTCTTCATTTGATCCTGCCAAGCAAAGTAGATCCCC
TGGTCAACCTTATGAAGGTTGAAAAGGTCCCTGATTCTACATATGACATG
ATTGGTGGCCTTGATCAGCAAATTAAGGAGATCAAGGAGGTCATTGAGCT
TCCTATCAAACATCCCCGAATTGTTCGAGTCTCTTGGAATAGCTCAACCC
AGGGGTGTATTGCTATAT
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