CN181794

Overview
NameCN181794
Unique NameCN181794
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length668
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_ARATH (26S protease regulatory subunit 6B homolog OS=Arabidopsis thaliana GN=RPT3 PE=1 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 5.576e-12
Identity = 35/129 (27.13%), Postives = 64/129 (49.61%), Query Frame = 2 
Query:  242 QRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRIVR 628
            ++  L   +   +EE++ +Q     +G+ ++++ +N  +V       Y V            PS  VAL   S  L  +LP + D  ++L+   + PD +Y+ IGG D Q +EI+E +ELP+ H  + +
Sbjct:   54 EQKNLKRELLRAQEEVKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSEKPDVSYNDIGGCDIQKQEIREAVELPLTHHELYK 182

HSP 2 Score: 27.335 bits (59), Expect = 5.576e-12
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVLLY
Sbjct:  179 ELYKQIGIDPPRGVLLY 195
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_SCHPO (26S protease regulatory subunit 6B homolog OS=Schizosaccharomyces pombe GN=rpt3 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 9.415e-12
Identity = 43/156 (27.56%), Postives = 74/156 (47.44%), Query Frame = 2 
Query:  188 ELQLQVRQKT--HNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHPRIVR 628
            E+ L  R K     L  L+ Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       YVV            PS  VAL+  S  L  ILP + D  +++++ ++ PD +Y  +GGLD Q +E++E +ELP+    + R
Sbjct:    6 EIDLYNRWKALERQLEMLDLQEGFIKEDCKSLKRELIRAQEEVKRIQSVPLVIGQFLEAIDQNTAIVGSTTGSNYVVRILSTLDRELLKPSASVALQRHSNALVDILPPEADSSISMLRPDERPDVSYADVGGLDVQKQEVREAVELPLTQGDLYR 161

HSP 2 Score: 25.7942 bits (55), Expect = 9.415e-12
Identity = 10/17 (58.82%), Postives = 13/17 (76.47%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            +L+  +GI  PRGVLLY
Sbjct:  158 DLYRQIGIDPPRGVLLY 174
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS7_YEAST (26S protease regulatory subunit 7 homolog OS=Saccharomyces cerevisiae GN=RPT1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.048e-11
Identity = 30/89 (33.71%), Postives = 47/89 (52.81%), Query Frame = 2 
Query:  350 KVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            K ++ +    K+VV               RV +    Y + L LP ++DP V +M VE+ PD TY  +GG   QI++++EV+ELP+  P
Sbjct:  144 KYVINLKQIAKFVVGLGERVSPTDIEEGMRVGVDRSKYNIELPLPPRIDPSVTMMTVEEKPDVTYSDVGGCKDQIEKLREVVELPLLSP 232

HSP 2 Score: 27.7202 bits (60), Expect = 2.048e-11
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            PE F +LGI  P+G+LLY
Sbjct:  232 PERFATLGIDPPKGILLY 249
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_RAT (26S protease regulatory subunit 6B OS=Rattus norvegicus GN=Psmc4 PE=1 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.463e-11
Identity = 38/153 (24.84%), Postives = 70/153 (45.75%), Query Frame = 2 
Query:  176 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKH 613
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTH 187

HSP 2 Score: 26.5646 bits (57), Expect = 3.463e-11
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVL+Y
Sbjct:  189 ELYKQIGIDPPRGVLMY 205
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_MACFA (26S protease regulatory subunit 6B OS=Macaca fascicularis GN=PSMC4 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.463e-11
Identity = 38/153 (24.84%), Postives = 70/153 (45.75%), Query Frame = 2 
Query:  176 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKH 613
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTH 187

HSP 2 Score: 26.5646 bits (57), Expect = 3.463e-11
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVL+Y
Sbjct:  189 ELYKQIGIDPPRGVLMY 205
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_HUMAN (26S protease regulatory subunit 6B OS=Homo sapiens GN=PSMC4 PE=1 SV=2)

HSP 1 Score: 61.6178 bits (148), Expect = 3.463e-11
Identity = 38/153 (24.84%), Postives = 70/153 (45.75%), Query Frame = 2 
Query:  176 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKH 613
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTH 187

HSP 2 Score: 26.5646 bits (57), Expect = 3.463e-11
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVL+Y
Sbjct:  189 ELYKQIGIDPPRGVLMY 205
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_BOVIN (26S protease regulatory subunit 6B OS=Bos taurus GN=PSMC4 PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 3.463e-11
Identity = 38/153 (24.84%), Postives = 70/153 (45.75%), Query Frame = 2 
Query:  176 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKH 613
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP + D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTH 187

HSP 2 Score: 26.5646 bits (57), Expect = 3.463e-11
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVL+Y
Sbjct:  189 ELYKQIGIDPPRGVLMY 205
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PSMR2_HALSA (Proteasome-activating nucleotidase 2 OS=Halobacterium salinarium GN=pan2 PE=3 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 3.467e-11
Identity = 40/155 (25.81%), Postives = 76/155 (49.03%), Query Frame = 2 
Query:  161 RQYYLQHIHELQLQVRQKTHNLNRL---EAQRNELNSRVRMLKEELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKHP 616
            +Q  L+ + E    + ++    +RL    A+ N+   ++  L  E + L++   ++  V ++  +  V +K H   +  +            P +RVA+ N   V+   L  + D    +M+V+  PD  Y+ IGGLD Q++E++E +ELP+K P
Sbjct:   20 QQDKLEALEEQLSTLEEENEERDRLLDANAENNKYQQKLERLSHENKKLKQSPLFIATVQELTDEGAV-IKQHGNNQEALTEVTDELRATLEPGSRVAVNNSLSVVRQ-LDDEADVRARVMEVDDSPDVGYEDIGGLDDQLREVRETVELPMKDP 172

HSP 2 Score: 25.7942 bits (55), Expect = 3.467e-11
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 3 
Query:  615 PELFESLGIAQPRGVLLY 668
            P LFE++GI  P GVLL+
Sbjct:  172 PGLFETVGINPPSGVLLH 189
[Close]
BLAST of CN181794 vs. ExPASy Swiss-Prot
Match: PRS6B_MOUSE (26S protease regulatory subunit 6B OS=Mus musculus GN=Psmc4 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.496e-11
Identity = 38/153 (24.84%), Postives = 69/153 (45.10%), Query Frame = 2 
Query:  176 QHIHELQLQVRQKTHNLNRLEAQRNELNSRVRMLK-------EELQLLQEPGSYVGEVVKVMGKNKVLVKVHPEGKYVVXXXXXXXXXXXXPSTRVALRNDSYVLHLILPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKEVIELPIKH 613
            + + +L  + ++    L  LE Q   +    + LK       EE++ +Q     +G+ ++ + +N  +V       Y V            P+  VAL   S  L  +LP   D  + ++  ++ PD  Y  IGG+D Q +E++E +ELP+ H
Sbjct:   35 EDLEDLYSRYKKLQQELEFLEVQEEYIKDEQKNLKKEFLHAQEEVKRIQSIPLVIGQFLEAVDQNTAIVASTTGSNYYVRMVSTIDRELLKPNASVALHKHSNALVDVLPPAADSSIMMLTSDQKPDVMYADIGGMDIQKQEVREAVELPLTH 187

HSP 2 Score: 26.5646 bits (57), Expect = 4.496e-11
Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = 3 
Query:  618 ELFESLGIAQPRGVLLY 668
            EL++ +GI  PRGVL+Y
Sbjct:  189 ELYKQIGIDPPRGVLMY 205
The following BLAST results are available for this feature:
BLAST of CN181794 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
PRS6B_ARATH5.576e-1227.1326S protease regulatory subunit 6B homolog OS=Arab... [more]
PRS6B_SCHPO9.415e-1227.5626S protease regulatory subunit 6B homolog OS=Schi... [more]
PRS7_YEAST2.048e-1133.7126S protease regulatory subunit 7 homolog OS=Sacch... [more]
PRS6B_RAT3.463e-1124.8426S protease regulatory subunit 6B OS=Rattus norve... [more]
PRS6B_MACFA3.463e-1124.8426S protease regulatory subunit 6B OS=Macaca fasci... [more]
PRS6B_HUMAN3.463e-1124.8426S protease regulatory subunit 6B OS=Homo sapiens... [more]
PRS6B_BOVIN3.463e-1124.8426S protease regulatory subunit 6B OS=Bos taurus G... [more]
PSMR2_HALSA3.467e-1125.81Proteasome-activating nucleotidase 2 OS=Halobacter... [more]
PRS6B_MOUSE4.496e-1124.8426S protease regulatory subunit 6B OS=Mus musculus... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001H20_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001H20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181794 ID=CN181794; Name=CN181794; organism=Citrus sinensis; type=EST; length=668bp
GAGGCGAGACTTGATCCGAAAGACAATTAGATTCGAACAAATCGAACTCG
TTCACTCGGAAAGAATGGCCGCTGTAGGAATCGAAACGAAGCAGCCGGAA
TCGGCGGCGCCAGAGGAGGTGTGTGCGGCGAAAGGAGCATCGAAACAAGG
AGAGGGATTGAGGCAATACTACCTCCAACACATTCACGAACTCCAGCTCC
AAGTCCGTCAAAAAACCCACAATCTCAATCGCCTCGAAGCCCAGCGTAAC
GAACTCAATTCTAGAGTGCGGATGCTTAAAGAAGAATTACAGCTTCTTCA
AGAGCCTGGATCCTATGTCGGTGAAGTTGTTAAAGTTATGGGAAAAAATA
AGGTTCTTGTTAAGGTTCATCCTGAGGGGAAATATGTTGTTGATATTGAT
AAAAGTATTGATATAACCAAGATCACTCCGTCAACAAGAGTTGCACTTCG
TAATGACAGCTATGTTCTTCATTTGATCCTGCCAAGCAAAGTAGATCCCC
TGGTCAACCTTATGAAGGTTGAAAAGGTCCCTGATTCTACATATGACATG
ATTGGTGGCCTTGATCAGCAAATTAAGGAGATCAAGGAGGTCATTGAGCT
TCCTATCAAACATCCCCGAATTGTTCGAGTCTCTTGGAATAGCTCAACCC
AGGGGTGTATTGCTATAT
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