CN181761

Overview
NameCN181761
Unique NameCN181761
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length643
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_CHICK (Elongation factor 2 OS=Gallus gallus GN=EEF2 PE=1 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 3.175e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDSASRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 3.175e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_RAT (Elongation factor 2 OS=Rattus norvegicus GN=Eef2 PE=1 SV=4)

HSP 1 Score: 99.3673 bits (246), Expect = 4.134e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 4.134e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_PONAB (Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 SV=3)

HSP 1 Score: 99.3673 bits (246), Expect = 4.134e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 4.134e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_MOUSE (Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 SV=2)

HSP 1 Score: 99.3673 bits (246), Expect = 4.134e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 4.134e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_MESAU (Elongation factor 2 OS=Mesocricetus auratus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 99.3673 bits (246), Expect = 4.134e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 4.134e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_HUMAN (Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4)

HSP 1 Score: 99.3673 bits (246), Expect = 4.134e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 4.134e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_CALJA (Elongation factor 2 OS=Callithrix jacchus GN=EEF2 PE=2 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 7.007e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 7.007e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEESQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_BOVIN (Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=3)

HSP 1 Score: 98.5969 bits (244), Expect = 7.007e-29
Identity = 45/79 (56.96%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP +  ++ +Q+VA+ RKRKGLKE +  L  F DKL
Sbjct:  780 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNTSRPSQVVAETRKRKGLKEGIPALDNFLDKL 858

HSP 2 Score: 48.1358 bits (113), Expect = 7.007e-29
Identity = 21/29 (72.41%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            EQ +GGIY VLN+KRGHVFEE Q  GTP+
Sbjct:  753 EQVVGGIYGVLNRKRGHVFEETQVAGTPM 781
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_DROME (Elongation factor 2 OS=Drosophila melanogaster GN=Ef2b PE=1 SV=4)

HSP 1 Score: 101.293 bits (251), Expect = 9.124e-29
Identity = 46/79 (58.23%), Postives = 58/79 (73.42%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+  LR+ T GQAFPQCVFDHW ++  DP EP ++   +V D RKRKGLKE +  LS++ DKL
Sbjct:  766 PMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPSEPSSKPYAIVQDTRKRKGLKEGLPDLSQYLDKL 844

HSP 2 Score: 45.0542 bits (105), Expect = 9.124e-29
Identity = 20/29 (68.97%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            E A+GGIY VLN++RGHVFEE Q  GTP+
Sbjct:  739 EVAVGGIYGVLNRRRGHVFEENQVVGTPM 767
[Close]
BLAST of CN181761 vs. ExPASy Swiss-Prot
Match: EF2_SACCA (Elongation factor 2 OS=Saccharomyces castellii GN=EFT1 PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 9.124e-29
Identity = 45/79 (56.96%), Postives = 57/79 (72.15%), Query Frame = -3 
Query:  324 PFYNIKAYLPVIESFGFSGTLRAATSGQAFPQCVFDHWDMMSSDPLEPGTQAAQLVADIRKRKGLKEQMTPLSEFEDKL 560
            P + +KAYLPV ESFGF+G LR AT GQAFPQ VFDHW  + SDPL+P ++A ++V   RKR G+KE +    E+ DKL
Sbjct:  764 PLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHWATLGSDPLDPTSKAGEIVTAARKRHGMKEVVPGWQEYYDKL 842

HSP 2 Score: 48.1358 bits (113), Expect = 9.124e-29
Identity = 22/29 (75.86%), Postives = 24/29 (82.76%), Query Frame = -2 
Query:  556 EQALGGIYSVLNQKRGHVFEEMQRPGTPL 642
            E A+GGIYSVLN+KRG V  E QRPGTPL
Sbjct:  737 ESAVGGIYSVLNKKRGQVVSEEQRPGTPL 765
The following BLAST results are available for this feature:
BLAST of CN181761 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 36
Match NameE-valueIdentityDescription
EF2_CHICK3.175e-2956.96Elongation factor 2 OS=Gallus gallus GN=EEF2 PE=1 ... [more]
EF2_RAT4.134e-2956.96Elongation factor 2 OS=Rattus norvegicus GN=Eef2 P... [more]
EF2_PONAB4.134e-2956.96Elongation factor 2 OS=Pongo abelii GN=EEF2 PE=2 S... [more]
EF2_MOUSE4.134e-2956.96Elongation factor 2 OS=Mus musculus GN=Eef2 PE=1 S... [more]
EF2_MESAU4.134e-2956.96Elongation factor 2 OS=Mesocricetus auratus GN=EEF... [more]
EF2_HUMAN4.134e-2956.96Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 S... [more]
EF2_CALJA7.007e-2956.96Elongation factor 2 OS=Callithrix jacchus GN=EEF2 ... [more]
EF2_BOVIN7.007e-2956.96Elongation factor 2 OS=Bos taurus GN=EEF2 PE=2 SV=... [more]
EF2_DROME9.124e-2958.23Elongation factor 2 OS=Drosophila melanogaster GN=... [more]
EF2_SACCA9.124e-2956.96Elongation factor 2 OS=Saccharomyces castellii GN=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001H02_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001H02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181761 ID=CN181761; Name=CN181761; organism=Citrus sinensis; type=EST; length=643bp
GGCCAGAATTAGTTTAAACACATGACAGAACATCAAACCTTAAAAGCTGT
GAATTGTAAAATTTAAAACCTAGATGACAGAGACAGGGTTACTAAACCCA
ATCAACAGTAATATTACTTTACTAAACACAATGAGGATATTGAAAAACTT
TGAAACAAAAATAAGCATCAGCAAAAAATATTGCTGCGCAATCTCACCCT
TGATGTCAAGATGAAATGTTTACAGCTCGTCCTCTACTCGGATAGCGGTG
TCATTTGCTCCCTCTTCCTGATATCAGCAACAAGCTGCGCAATCTCACCC
TTGATGTCAAGACGAAATGTTTACAGCTTGTCCTCAAACTCAGATAGTGG
TGTCATTTGCTCCTTCAAGCCCTTCCTCTTCCTGATATCAGCAACAAGCT
GTGCAGCCTGTGTACCAGGCTCCAGTGGATCGGATGACATCATGTCCCAG
TGATCAAACACACATTGTGGGAAGGCCTGTCCTGACGTTGCAGCCCTCAA
AGTGCCTGAGAATCCAAAGGACTCGATGACGGGAAGGTAAGCCTTGATGT
TGTAAAAGGGGGGTACCAGGTCTCTGCATTTCCTCGAACACGTGACCACG
TTTCTGATTGAGAACACTGTAAATACCACCCAAGGCCTGCTCG
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