CN188641

Overview
NameCN188641
Unique NameCN188641
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length734
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2111 contig Csv1_Contig2111:99..831. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UB2D3_BOVIN (Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 255.373 bits (651), Expect = 2.359e-67
Identity = 117/147 (79.59%), Postives = 127/147 (86.39%), Query Frame = 1
Query:   97 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYAM 537
            MA KRI KEL DL +DPP  CSAGPV +DMFHWQATIMGP DSPY GGVF +TIHFP DYPFKPPKVAF T+++HPNINSN SICLDIL+ QWSPALTISKVLLSICSLL DPNPDDPLVPEIA +YKTDR+KY   +R WTQKYAM
Sbjct:    1 MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNDSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM 147          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 2.608e-66
Identity = 117/149 (78.52%), Postives = 134/149 (89.93%), Query Frame = 1
Query:   97 MASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYAMG 540
            MASKRI +EL+D+Q+ PP +CSAGPVAE D+FHWQATIMGP DSPY+GGVF V+I F  DYPFKPPKV F+TKV+HPNI+S GSICLDILKEQWSPA T SKVLLSICSLLTDPNP+DPLVPEIAH+YK D++KYE+TA+ WTQKYAMG
Sbjct:    1 MASKRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UBC5_YEAST (Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharomyces cerevisiae GN=UBC5 PE=1 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 3.407e-66
Identity = 112/146 (76.71%), Postives = 131/146 (89.73%), Query Frame = 1
Query:  100 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYAM 537
            +SKRI KEL DL +DPP SCSAGPV +D++HWQA+IMGP DSPYAGGVF ++IHFP DYPFKPPKV F TK++HPNINS+G+ICLDILK+QWSPALT+SKVLLSICSLLTD NPDDPLVPEIA +YKTD+ KYE TA+ WT+KYA+
Sbjct:    3 SSKRIAKELSDLGRDPPASCSAGPVGDDLYHWQASIMGPSDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINSSGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAQIYKTDKAKYEATAKEWTKKYAV 148          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: U2D2B_RAT (Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norvegicus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 1.295e-65
Identity = 115/147 (78.23%), Postives = 125/147 (85.03%), Query Frame = 1
Query:   97 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYAM 537
            MA KRI KEL DL +DPP  CSAGPV EDMFHWQATIMGP DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YKTDR+KY  TAR WTQKYAM
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: U2D2B_MOUSE (Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculus GN=Ube2d2b PE=2 SV=1)

HSP 1 Score: 249.595 bits (636), Expect = 1.295e-65
Identity = 115/147 (78.23%), Postives = 125/147 (85.03%), Query Frame = 1
Query:   97 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYAM 537
            MA KRI KEL DL +DPP  CSAGPV EDMFHWQATIMGP DSPY GG F +TI FP +YPFKPPKV F T+++HPN+NSNGSICLDIL+ QWSPALTISKVLLSI SLL DPNPDDPLVPEIA +YKTDR+KY  TAR WTQKYAM
Sbjct:    1 MALKRIHKELNDLAQDPPAQCSAGPVGEDMFHWQATIMGPNDSPYQGGAFFLTIDFPTEYPFKPPKVEFTTRIYHPNVNSNGSICLDILRSQWSPALTISKVLLSISSLLCDPNPDDPLVPEIAQIYKTDRDKYNRTAREWTQKYAM 147          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 6.896e-51
Identity = 95/158 (60.13%), Postives = 119/158 (75.32%), Query Frame = 1
Query:   61 KSGGSAASQVETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYA 534
            K     A+++ T A KRI KEL ++  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+R +++  AR WT++YA
Sbjct:   44 KISSKTAAKLSTSA-KRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 6.896e-51
Identity = 95/158 (60.13%), Postives = 119/158 (75.32%), Query Frame = 1
Query:   61 KSGGSAASQVETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYA 534
            K     A+++ T A KRI KEL ++  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F PDYPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+R +++  AR WT++YA
Sbjct:   44 KISSKTAAKLSTSA-KRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 200          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 6.896e-51
Identity = 93/154 (60.39%), Postives = 119/154 (77.27%), Query Frame = 1
Query:   73 SAASQVETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYA 534
            S  S++ + ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+R +++  AR WT++YA
Sbjct:   39 SKNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 200.675 bits (509), Expect = 6.896e-51
Identity = 93/154 (60.39%), Postives = 119/154 (77.27%), Query Frame = 1
Query:   73 SAASQVETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYA 534
            S  S++ + ++KRI KEL D+  DPP +CSAGP  ++++ W++TI+GPP S Y GGVF + I F P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y T+R +++  AR WT++YA
Sbjct:   39 SKNSKLLSTSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYA 192          
BLAST of CN188641 vs. ExPASy Swiss-Prot
Match: UBCD2_DROME (Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophila melanogaster GN=UbcD2 PE=1 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 4.470e-50
Identity = 90/145 (62.07%), Postives = 113/145 (77.93%), Query Frame = 1
Query:  100 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRNKYETTARSWTQKYA 534
            ++KRI KEL ++  DPP +CSAGP  ++++ W +TI+GPP S Y GGVF + IHF P+YPFKPPKV FRT+++H NINS G ICLDILK+ WSPALTISKVLLSICSLLTD NP DPLV  IA  Y  +R +++  AR WT++YA
Sbjct:   87 SAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLQNREEHDRIARLWTKRYA 231          
The following BLAST results are available for this feature:
BLAST of CN188641 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 267
Match NameE-valueIdentityDescription
UB2D3_BOVIN2.359e-6779.59Ubiquitin-conjugating enzyme E2 D3 OS=Bos taurus G... [more]
UBC12_ARATH2.608e-6678.52Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UBC5_YEAST3.407e-6676.71Ubiquitin-conjugating enzyme E2-16 kDa OS=Saccharo... [more]
U2D2B_RAT1.295e-6578.23Ubiquitin-conjugating enzyme E2 D2B OS=Rattus norv... [more]
U2D2B_MOUSE1.295e-6578.23Ubiquitin-conjugating enzyme E2 D2B OS=Mus musculu... [more]
UB2E2_MOUSE6.896e-5160.13Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN6.896e-5160.13Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UB2E1_MOUSE6.896e-5160.39Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN6.896e-5160.39Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]
UBCD2_DROME4.470e-5062.07Ubiquitin-conjugating enzyme E2-24 kDa OS=Drosophi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006H20_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006H20, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188641 ID=CN188641; Name=CN188641; organism=Citrus sinensis; type=EST; length=734bp
CCCTTTGTTCTATTTTCCCGATCTCCCTGTCTCCCTCACGAATCCACATA
TTGCACTTAAAAGTCAGGAGGATCTGCTGCTTCTCAGGTTGAAACCATGG
CGTCGAAACGGATCTTGAAGGAGCTCAAAGATTTGCAGAAGGATCCTCCT
ACATCATGCAGTGCCGGTCCTGTTGCTGAAGACATGTTTCATTGGCAGGC
GACAATCATGGGTCCTCCAGATAGTCCGTATGCTGGGGGTGTTTTTCTAG
TTACCATCCATTTTCCCCCAGACTATCCATTCAAGCCTCCCAAGGTAGCA
TTCAGGACAAAGGTATTTCATCCTAACATAAATAGCAATGGGAGCATTTG
CCTTGACATCTTGAAGGAACAATGGAGTCCTGCTCTTACCATTTCCAAGG
TGTTGCTATCAATCTGTTCATTATTGACGGATCCAAATCCCGATGATCCG
TTGGTACCAGAAATTGCTCACATGTACAAGACCGACAGGAACAAGTACGA
GACAACTGCAAGGAGCTGGACCCAGAAGTATGCCATGGGCTAAACCTGGG
TTATGGTGTGTGAGGGGGTTCCTTTTCTTTTTAATTCTGGTTTCGAGTTA
ATGTATGAATATTCTGTCTGTTCAATCCTAAAAAGTGGACCATAAGAAAG
GTCCACTACATCCCCCGAATTGGAAAGGAGAAACGGGTTGCTTCTTAATT
GTTGTATGGATAATGTCACTTTGGTCAATCAAAT
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