CN188628

Overview
NameCN188628
Unique NameCN188628
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length702
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig9569 contig Csv1_Contig9569:575..1276. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 9.954e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76          

HSP 2 Score: 97.0561 bits (240), Expect = 9.954e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76          

HSP 3 Score: 69.3218 bits (168), Expect = 2.223e-11
Identity = 31/54 (57.41%), Postives = 40/54 (74.07%), Query Frame = 2
Query:    8 IDNVKAKIQDKAGIPPDQQRLIFAGKQLEDGRTLAPYNIQKESTLHLVLRLRGG 169
            ++ +K K+++K GIPP QQRLIFAGKQ+ D +T A Y +   S LHLVL LRGG
Sbjct:   23 VERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.300e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 2
Query:  176 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 400
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77          

HSP 2 Score: 96.6709 bits (239), Expect = 1.300e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 2
Query:  404 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 628
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77          

HSP 3 Score: 70.4774 bits (171), Expect = 9.977e-12
Identity = 33/56 (58.93%), Postives = 42/56 (75.00%), Query Frame = 2
Query:    5 TIDNVKAKIQDKAGIPPDQQRLIFAGKQLEDGRTLAPYNIQKESTLHLVLRLRGGM 172
            TID +K ++++K GIPP QQRLI+AGKQL D +T   Y I+  S LHLVL LRGG+
Sbjct:   22 TIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          

HSP 2 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          

HSP 2 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          

HSP 2 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          

HSP 2 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          

HSP 2 Score: 93.2041 bits (230), Expect = 1.437e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.877e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76          

HSP 2 Score: 92.8189 bits (229), Expect = 1.877e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76          

HSP 3 Score: 67.3958 bits (163), Expect = 8.446e-11
Identity = 30/55 (54.55%), Postives = 40/55 (72.73%), Query Frame = 2
Query:    5 TIDNVKAKIQDKAGIPPDQQRLIFAGKQLEDGRTLAPYNIQKESTLHLVLRLRGG 169
            T+D +K +++++ GIPP QQRLI+ GKQL D +T   Y I+  S LHLVL LRGG
Sbjct:   22 TVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.217e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 397
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76          

HSP 2 Score: 90.1225 bits (222), Expect = 1.217e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 625
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76          
BLAST of CN188628 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 1.589e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 2
Query:  170 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 400
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77          

HSP 2 Score: 89.7373 bits (221), Expect = 1.589e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 2
Query:  398 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 628
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77          
The following BLAST results are available for this feature:
BLAST of CN188628 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
NEDD8_CAEEL9.954e-2060.53NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ARATH1.300e-1961.33NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
NEDD8_RAT1.437e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.437e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.437e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.437e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.437e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ORYSJ1.877e-1853.95NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
RUB3_ARATH1.217e-1755.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI1.589e-1753.25NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006H02_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006H02, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188628 ID=CN188628; Name=CN188628; organism=Citrus sinensis; type=EST; length=702bp
CGTCACCATTGACAATGTCAAAGCCAAAATTCAGGATAAGGCTGGGATCC
CACCAGACCAGCAAAGGCTCATCTTTGCCGGTAAACAACTAGAGGATGGA
CGCACACTTGCTCCCTACAACATCCAGAAGGAATCAACCCTTCATTTGGT
CCTCCGTCTCAGGGGTGGCATGCAGATTTTTGTGAAAACCCTGACTGGTA
AGACTATAACTTTGGAGGTTGAAAGCTCTGACACGATCGACAATGTCAAG
GCCAAGATCCAGGACAAGGAGGGCATCCCACCTGATCAACAGAGGTTAAT
CTTTGCCGGCAAACAACTTGAAGATGGTCGTACTCTTGCTGACTACAACA
TCCAGAAGGAGTCAACCCTCCATTTGGTGCTTCGTCTCCGTGGTGGCATG
CAGATCTTTGTGAAAACCCTCACCGGAAAAACAATTACTCTTGAGGTTGA
AAGTTCTGACACCATCGACAACGTGAAAGCAAAAATTCAAGACAAGGAGG
GTATCCCACCAGATCAGCAGAGGCTCATTTTTGCTGGAAAGCAGCTTGAA
GATGGAAGGACTCTTGCAGACTATAACATCCAGAAAGAATCCACTCTTCA
CTTGGTTCTTCGCCTCCGTGGTGGTATGCAGATTTTTGTGAAGACACTCA
CTGGGAAAACCATCACATTGGAGGTTGAGAGTTCGGACACAATTGACAAT
GT
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