CN188581

Overview
NameCN188581
Unique NameCN188581
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length719
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig7124 contig Csv1_Contig7124:365..1083. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_PANTR (Elongation factor 1-alpha 1 OS=Pan troglodytes GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_MOUSE (Elongation factor 1-alpha 1 OS=Mus musculus GN=Eef1a1 PE=1 SV=3)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_HUMAN (Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF1A1 PE=1 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_HORSE (Elongation factor 1-alpha 1 OS=Equus caballus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_FELCA (Elongation factor 1-alpha 1 OS=Felis catus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_CRIGR (Elongation factor 1-alpha 1 OS=Cricetulus griseus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A1_BOVIN (Elongation factor 1-alpha 1 OS=Bos taurus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 359.762 bits (922), Expect = 8.541e-99
Identity = 182/249 (73.09%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A2_XENLA (Elongation factor 1-alpha, oocyte form OS=Xenopus laevis GN=eef1ao PE=1 SV=1)

HSP 1 Score: 358.992 bits (920), Expect = 1.457e-98
Identity = 179/249 (71.89%), Postives = 208/249 (83.53%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVLI+    G FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P +S+ R++EI KEVS+Y+KK+GYNP  VPFVPISG+ GDNM+E STN+ W+KG             TLL+ALD I  P+RP+ KPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGM+VTF PS +TTEVKSVEMHHEALQEALPGDNVGFNVKN++VKD++RG VA +SK+DP  +A +FT+QVII
Sbjct:   98 FIKNMITGTSQADCAVLIVAGGVGEFEAGISKNGQTREHALLAFTLGVKQLIIGVNKMDSTEPPFSQKRFEEITKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEASTNMPWFKGWKIERKEGNASGVTLLEALDCIIPPQRPTAKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMIVTFAPSNVTTEVKSVEMHHEALQEALPGDNVGFNVKNISVKDIRRGNVAGDSKNDPPMQAGSFTAQVII 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A2_OSCTI (Elongation factor 1-alpha 2 OS=Oscheius tipulae GN=eft-2 PE=3 SV=1)

HSP 1 Score: 358.607 bits (919), Expect = 1.903e-98
Identity = 175/249 (70.28%), Postives = 207/249 (83.13%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQADCAVL++   TG FEAGISK+GQTREHALLA TLGVKQMI  CNKMD+T P +S+ R++EI+ EV S++KK+GYNP  +PFVPISGF GDNM+E S N+ WYKG             TLL+ALD I  P+RP+D+PLRLPLQDVYKIGGIGTVPVGRVETGV+KPGMVVTF P  +TTEVKSVEMHHE+L EA PGDNVGFNVKNV+VKD++RG V S+SK+DPA+E+ +FT+QVI+
Sbjct:   98 FIKNMITGTSQADCAVLVVACGTGEFEAGISKNGQTREHALLAQTLGVKQMIVACNKMDSTEPPFSEKRFEEIITEVKSFIKKIGYNPATIPFVPISGFNGDNMLEPSANMSWYKGWSVERKEGNASGKTLLEALDCIIPPQRPTDRPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPQNVTTEVKSVEMHHESLPEAQPGDNVGFNVKNVSVKDIRRGSVCSDSKNDPAKESKSFTAQVIV 346
[Close]
BLAST of CN188581 vs. ExPASy Swiss-Prot
Match: EF1A_TETPY (Elongation factor 1-alpha OS=Tetrahymena pyriformis PE=2 SV=1)

HSP 1 Score: 357.836 bits (917), Expect = 3.246e-98
Identity = 174/237 (73.42%), Postives = 199/237 (83.97%), Query Frame = 1 
Query:    7 FIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVII 717
            FIKNMITGTSQAD A+L+I S  G FEAGISKDGQTREHALLAFTLGVKQMI C NKMD  T  +S+ RY EI KE+S YLKKVGY P+ +PF+PISGF GDNM+ERSTN  WYKGP L++ALD +  PKRP DKPLRLPLQDVYKIGGIGTVPVGRVETGV+KPGM + F P+ +  E KSVEMHHE L EA+PGDNVGFN+K V+VKD++RG VAS++K+DPA+EAA F SQVII
Sbjct:   99 FIKNMITGTSQADVAILMIASPQGEFEAGISKDGQTREHALLAFTLGVKQMIVCLNKMDEKTVNFSEERYQEIKKELSDYLKKVGYKPDTIPFIPISGFNGDNMLERSTNAPWYKGPILVEALDALEPPKRPVDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMSIQFAPNKVIAECKSVEMHHEQLPEAVPGDNVGFNIKGVSVKDIRRGNVASDAKNDPAKEAATFYSQVII 335
The following BLAST results are available for this feature:
BLAST of CN188581 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A1_PANTR8.541e-9973.09Elongation factor 1-alpha 1 OS=Pan troglodytes GN=... [more]
EF1A1_MOUSE8.541e-9973.09Elongation factor 1-alpha 1 OS=Mus musculus GN=Eef... [more]
EF1A1_HUMAN8.541e-9973.09Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF... [more]
EF1A1_HORSE8.541e-9973.09Elongation factor 1-alpha 1 OS=Equus caballus GN=E... [more]
EF1A1_FELCA8.541e-9973.09Elongation factor 1-alpha 1 OS=Felis catus GN=EEF1... [more]
EF1A1_CRIGR8.541e-9973.09Elongation factor 1-alpha 1 OS=Cricetulus griseus ... [more]
EF1A1_BOVIN8.541e-9973.09Elongation factor 1-alpha 1 OS=Bos taurus GN=EEF1A... [more]
EF1A2_XENLA1.457e-9871.89Elongation factor 1-alpha, oocyte form OS=Xenopus ... [more]
EF1A2_OSCTI1.903e-9870.28Elongation factor 1-alpha 2 OS=Oscheius tipulae GN... [more]
EF1A_TETPY3.246e-9873.42Elongation factor 1-alpha OS=Tetrahymena pyriformi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006F16_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006F16, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188581 ID=CN188581; Name=CN188581; organism=Citrus sinensis; type=EST; length=719bp
AGTGTTTTTATCAAGAACATGATTACCGGTACCTCACAGGCTGACTGTGC
TGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAGCTGGTATCTCCA
AGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTCACCCTTGGTGTG
AAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCACCACCCCCAAGTA
TTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCTCTTCCTACCTGA
AGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTGCCCATCTCTGGT
TTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCTTGACTGGTACAA
GGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATGAGCCGAAGAGGC
CCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTGTACAAGATTGGT
GGCATTGGAACCGTCCCAGTGGGTCGCGTTGAGACCGGTGTCCTCAAGCC
TGGTATGGTGGTGACATTTGGCCCCTCTGGATTGACAACTGAGGTTAAGT
CTGTTGAGATGCACCACGAAGCTCTGCAGGAGGCCCTTCCGGGTGACAAT
GTTGGCTTCAACGTGAAGAACGTTGCTGTCAAGGATCTCAAGCGTGGTTT
TGTTGCTTCCAACTCGAAGGATGATCCAGCCAGGGAAGCTGCCAACTTCA
CCTCACAGGTCATCATCAT
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