CN181483

Overview
NameCN181483
Unique NameCN181483
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length341
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5956 contig Csv1_Contig5956:138..477. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_ORYSJ (Proliferating cell nuclear antigen OS=Oryza sativa subsp. japonica GN=Os02g0805200 PE=2 SV=2)

HSP 1 Score: 185.267 bits (469), Expect = 7.106e-48
Identity = 88/101 (87.13%), Postives = 95/101 (94.06%), Query Frame = 3 
Query:   39 FSLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEYHA 341
            FSLQ+MDSSHVALVALLLRSEGFEHYRCDRN+SMGMNLNN++KML+CAGNDDIIT+KADDGSDT T MFESP QDKIADFEMKLMDID EHLGIP +EY A
Sbjct:   35 FSLQAMDSSHVALVALLLRSEGFEHYRCDRNLSMGMNLNNMAKMLRCAGNDDIITIKADDGSDTVTFMFESPNQDKIADFEMKLMDIDSEHLGIPDSEYQA 135

HSP 2 Score: 26.9498 bits (58), Expect = 7.106e-48
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 2 
Query:    8 DANFDCSATGF 40
            DANFDCS TGF
Sbjct:   25 DANFDCSGTGF 35
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA2_DAUCA (Proliferating cell nuclear antigen large form OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 176.792 bits (447), Expect = 4.904e-46
Identity = 83/101 (82.18%), Postives = 92/101 (91.09%), Query Frame = 3 
Query:   39 FSLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEYHA 341
            FSLQ+MDSSHVALVA+LLRSEGFEHYRCDRNISMGMNL N++KML+CAGNDDI+T+KADD  D  T MFESPTQDKI+DFEMKLMDID EHLGIP +EY A
Sbjct:   35 FSLQAMDSSHVALVAVLLRSEGFEHYRCDRNISMGMNLGNMAKMLRCAGNDDIVTMKADDDGDVITFMFESPTQDKISDFEMKLMDIDSEHLGIPESEYEA 135

HSP 2 Score: 29.261 bits (64), Expect = 4.904e-46
Identity = 11/12 (91.67%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:    5 NDANFDCSATGF 40
            NDANFDCSA+GF
Sbjct:   24 NDANFDCSASGF 35
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_COTJA (Proliferating cell nuclear antigen OS=Coturnix coturnix japonica GN=PCNA PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 3.913e-36
Identity = 69/98 (70.41%), Postives = 87/98 (88.78%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            SLQSMDSSHV+LV L LRSEGF+ YRCDRNI+MG+NLN++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 SLQSMDSSHVSLVQLTLRSEGFDTYRCDRNIAMGVNLNSMSKILKCAGNEDIITLRAEDNADTLALVFETPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_CHICK (Proliferating cell nuclear antigen OS=Gallus gallus GN=PCNA PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 3.913e-36
Identity = 69/98 (70.41%), Postives = 87/98 (88.78%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            SLQSMDSSHV+LV L LRSEGF+ YRCDRNI+MG+NLN++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 SLQSMDSSHVSLVQLTLRSEGFDTYRCDRNIAMGVNLNSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_RAT (Proliferating cell nuclear antigen OS=Rattus norvegicus GN=Pcna PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.380e-35
Identity = 66/98 (67.35%), Postives = 86/98 (87.76%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            +LQSMDSSHV+LV L LRSEGF+ YRCDRN++MG+NL ++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_MOUSE (Proliferating cell nuclear antigen OS=Mus musculus GN=Pcna PE=1 SV=2)

HSP 1 Score: 145.591 bits (366), Expect = 7.380e-35
Identity = 66/98 (67.35%), Postives = 86/98 (87.76%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            +LQSMDSSHV+LV L LRSEGF+ YRCDRN++MG+NL ++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_MACFA (Proliferating cell nuclear antigen OS=Macaca fascicularis GN=PCNA PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.380e-35
Identity = 66/98 (67.35%), Postives = 86/98 (87.76%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            +LQSMDSSHV+LV L LRSEGF+ YRCDRN++MG+NL ++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_HUMAN (Proliferating cell nuclear antigen OS=Homo sapiens GN=PCNA PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.380e-35
Identity = 66/98 (67.35%), Postives = 86/98 (87.76%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            +LQSMDSSHV+LV L LRSEGF+ YRCDRN++MG+NL ++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_CRIGR (Proliferating cell nuclear antigen OS=Cricetulus griseus GN=PCNA PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.380e-35
Identity = 66/98 (67.35%), Postives = 86/98 (87.76%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            +LQSMDSSHV+LV L LRSEGF+ YRCDRN++MG+NL ++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
[Close]
BLAST of CN181483 vs. ExPASy Swiss-Prot
Match: PCNA_BOVIN (Proliferating cell nuclear antigen OS=Bos taurus GN=PCNA PE=2 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.380e-35
Identity = 66/98 (67.35%), Postives = 86/98 (87.76%), Query Frame = 3 
Query:   42 SLQSMDSSHVALVALLLRSEGFEHYRCDRNISMGMNLNNVSKMLKCAGNDDIITLKADDGSDTATLMFESPTQDKIADFEMKLMDIDREHLGIPHAEY 335
            +LQSMDSSHV+LV L LRSEGF+ YRCDRN++MG+NL ++SK+LKCAGN+DIITL+A+D +DT  L+FE+P Q+K++D+EMKLMD+D E LGIP  EY
Sbjct:   36 NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEY 133
The following BLAST results are available for this feature:
BLAST of CN181483 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 35
Match NameE-valueIdentityDescription
PCNA_ORYSJ7.106e-4887.13Proliferating cell nuclear antigen OS=Oryza sativa... [more]
PCNA2_DAUCA4.904e-4682.18Proliferating cell nuclear antigen large form OS=D... [more]
PCNA_COTJA3.913e-3670.41Proliferating cell nuclear antigen OS=Coturnix cot... [more]
PCNA_CHICK3.913e-3670.41Proliferating cell nuclear antigen OS=Gallus gallu... [more]
PCNA_RAT7.380e-3567.35Proliferating cell nuclear antigen OS=Rattus norve... [more]
PCNA_MOUSE7.380e-3567.35Proliferating cell nuclear antigen OS=Mus musculus... [more]
PCNA_MACFA7.380e-3567.35Proliferating cell nuclear antigen OS=Macaca fasci... [more]
PCNA_HUMAN7.380e-3567.35Proliferating cell nuclear antigen OS=Homo sapiens... [more]
PCNA_CRIGR7.380e-3567.35Proliferating cell nuclear antigen OS=Cricetulus g... [more]
PCNA_BOVIN7.380e-3567.35Proliferating cell nuclear antigen OS=Bos taurus G... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001A07_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001A07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181483 ID=CN181483; Name=CN181483; organism=Citrus sinensis; type=EST; length=341bp
GAGGAACGATGCCAACTTTGACTGCTCGGCCACCGGCTTTCTCTCTTCAA
TCCATGGACTCCAGCCACGTGGCGCTGGTGGCCCTGCTGCTCAGATCGGA
AGGCTTCGAGCACTACCGCTGTGACCGGAATATCTCTATGGGAATGAATC
TCAATAACGTGTCCAAGATGCTTAAGTGTGCTGGCAACGATGACATCATC
ACTCTCAAGGCCGACGACGGCAGCGATACCGCTACTCTCATGTTTGAAAG
TCCCACTCAAGATAAGATTGCTGATTTTGAGATGAAACTGATGGACATTG
ATAGAGAGCATCTTGGTATACCGCATGCTGAATACCATGCC
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