CN188561

Overview
NameCN188561
Unique NameCN188561
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length707
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PS10A_ARATH (26S protease regulatory subunit 10B homolog A OS=Arabidopsis thaliana GN=RPT4A PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.155e-23
Identity = 74/186 (39.78%), Postives = 99/186 (53.23%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI----AAQYAKHXXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            P+ VL  GPPGTGKT  AR IA+      + V    ++ KY GES RL+ ++F+ A E     IIF+DE+D+    R SE   A R   R L  LL Q+DGF+   KV +I ATNR   LDPAL+   R D  I   LP+ ++R +I    AA  AKH                +G D+R++C +A
Sbjct:  173 PKGVLLYGPPGTGKTLLARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFNYARE-HQPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQLDGFDNLGKVKMIMATNRPDVLDPALLRPGRLDRKIEIPLPNEQSRMDILKIHAAGIAKH-GEIDYEAIVKLAEGFNGADLRNICTEA 356          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: CDC48_SOYBN (Cell division cycle protein 48 homolog OS=Glycine max GN=CDC48 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.155e-23
Identity = 65/151 (43.05%), Postives = 85/151 (56.29%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEIAAQYAKH 521
            P+ +L  GPPG+GKT  AR +AN+ G     +    +MSK  GESE  L K F  A E    +IIF+DE+DS A  R+    E  RRI+S LL  +DG +    V+VI ATNR   +DPAL    RFD  I  G+PD   R E+   + K+
Sbjct:  242 PKGILLYGPPGSGKTLIARAVANETGAFFFCINGPEIMSKLAGESESNLRKAFEEA-EKNAPSIIFIDEIDSIAPKREKTHGEVERRIVSQLLTLMDGLKSRAHVIVIGATNRPNSIDPALRRFGRFDREIDIGVPDEVGRLEVLRIHTKN 391          

HSP 2 Score: 102.449 bits (254), Expect = 2.409e-21
Identity = 70/210 (33.33%), Postives = 106/210 (50.48%), Query Frame = 3
Query:   57 KFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHE---ATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI---AAQYAKHXXXXXXXXXXXXXXXMSGRDIRDVCQQAERSWASKIIRGQITKDGEQ 662
            KF  +  + VLF GPPG GKT  A+ IAN+     + V    +++ ++GESE  + ++F  A +     ++F DE+DS A  R S + +   A  R+L+ LL ++DG    K V +I ATNR   +DPAL+   R D +I   LPD ++R +I     + +                  SG DI ++CQ+A        IR  I KD E+
Sbjct:  509 KFGMSPSKGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVREIFDKARQ-SAPCVLFFDELDSIATQRGSSVGDAGGAADRVLNQLLTEMDGMSAKKTVFIIGATNRPDIIDPALLRPGRLDQLIYIPLPDEDSRHQIFKACLRKSPIAKNVDLRALARHTQGFSGADITEICQRA----CKYAIRENIEKDIER 713          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_METKA (Proteasome-activating nucleotidase OS=Methanopyrus kandleri GN=pan PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.971e-23
Identity = 74/207 (35.75%), Postives = 107/207 (51.69%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEAT------RRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PE+++ + +E         P+ VL  GPPGTGKT  A+ +AN A    + +    ++ K+ GE  RL+ ++F LA E    +IIF+DE+D+    R   M +AT      +R L+ LL ++DGF+    + VIAATNRK  LDPAL+   RFD  I   LPD E R EI   + +                    SG DI+ +C +A
Sbjct:  202 PELFEKVGVEP--------PKGVLLYGPPGTGKTLLAKAVANHADATFIRLAAPELVQKFIGEGARLVRELFELARE-KAPSIIFIDEIDAIGARR---MRDATSGDREVQRTLTQLLAEMDGFDPLDDIKVIAATNRKDILDPALLRPGRFDRHIKIPLPDEEGRYEIFKIHTRDMNLAEDVDLQKLAKITEGASGADIKAICTEA 396          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: FIGL1_CAEBR (Fidgetin-like protein 1 OS=Caenorhabditis briggsae GN=figl-1 PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.971e-23
Identity = 65/184 (35.33%), Postives = 100/184 (54.35%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLAN-ELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFE--QDKKVVVIAATNRKQDLDPALISRFDSMITFGLPDHENRQEIAAQYAKHXXXXXXXXXXXXXXXM----SGRDIRDVCQQA 605
            P+ VL  GPPGTGKT   R +A+QA      +    + SK+ GE E+L+  +FS+A  +LP  ++IF+DE+DS   AR    HE++RRI +  L Q+DG     D++++V+ ATNR Q+LD A   RF   +   LP+ ++R +I     +                +    SG D+R +C +A
Sbjct:  349 PKGVLLFGPPGTGKTMIGRCVASQAQATFFNISASSLTSKWVGEGEKLVRALFSVARLKLP--SVIFIDEIDSLLSARSESEHESSRRIKTEFLVQLDGVNTAPDERLLVLGATNRPQELDEAARRRFQKRLYIALPEPDSRTQIVENLLRGTRHEITDHNLEKIRRLTDGYSGADMRQLCTEA 530          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: CDC48_CAPAN (Cell division cycle protein 48 homolog OS=Capsicum annuum GN=CAFP PE=2 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.971e-23
Identity = 64/151 (42.38%), Postives = 85/151 (56.29%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEIAAQYAKH 521
            P+ +L  GPPG+GKT  AR +AN+ G     +    +MSK  GESE  L K F  A E    +IIF+DE+DS A  R+    E  RRI+S LL  +DG +    V+V+ ATNR   +DPAL    RFD  I  G+PD   R E+   + K+
Sbjct:  242 PKGILLYGPPGSGKTLIARAVANETGAFFFCINGPEIMSKLAGESESNLRKAFEEA-EKNAPSIIFIDEIDSIAPKREKTHGEVERRIVSQLLTLMDGLKSRAHVIVMGATNRPNSIDPALRRFGRFDREIDIGVPDEVGRLEVLGIHTKN 391          

HSP 2 Score: 101.293 bits (251), Expect = 5.366e-21
Identity = 69/210 (32.86%), Postives = 105/210 (50.00%), Query Frame = 3
Query:   57 KFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHE---ATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI---AAQYAKHXXXXXXXXXXXXXXXMSGRDIRDVCQQAERSWASKIIRGQITKDGEQ 662
            KF  +  + VLF GPPG GKT  A+ IAN+     + V    +++ ++GESE  + ++F  A +     ++F DE+DS A  R S   +   A  R+L+ LL ++DG    K V +I ATNR   +DPAL+   R D +I   LPD ++R +I     + +                  SG D+ ++CQ+A        IR  I KD E+
Sbjct:  509 KFGMSPSKGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVREIFDKARQ-SAPCVLFFDELDSIATQRGSSSGDAGGAADRVLNQLLTEMDGMNAKKTVFIIGATNRPDIIDPALLRPGRLDQLIYIPLPDEDSRHQIFKACLRKSPLSKDIDLRALAKHTQGFSGADVTEICQRA----CKYAIRENIEKDIER 713          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: TERA2_CAEEL (Transitional endoplasmic reticulum ATPase homolog 2 OS=Caenorhabditis elegans GN=cdc-48.2 PE=1 SV=2)

HSP 1 Score: 108.997 bits (271), Expect = 2.574e-23
Identity = 66/151 (43.71%), Postives = 86/151 (56.95%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEIAAQYAKH 521
            PR +L  GPPGTGKT  AR +AN+ G     +    +MSK  GESE  L K F+   E  + AI+F+DE+D+ A  R+    E  +RI+S LL  +DG +    VVVIAATNR   +D AL    RFD  I  G+PD   R EI   + K+
Sbjct:  243 PRGILLFGPPGTGKTLIARAVANETGAFFFLINGPEIMSKMSGESESNLRKAFAEC-EKNSPAILFIDEIDAIAPKREKAHGEVEKRIVSQLLTLMDGLKTRAHVVVIAATNRPNSIDGALRRFGRFDREIDIGIPDAVGRLEILRIHTKN 392          

HSP 2 Score: 95.9005 bits (237), Expect = 2.255e-19
Identity = 65/214 (30.37%), Postives = 105/214 (49.07%), Query Frame = 3
Query:   45 ETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHE---ATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI---AAQYAKHXXXXXXXXXXXXXXXMSGRDIRDVCQQAERSWASKIIRGQITKDGEQ 662
            E   KF     R VLF GPPG GKT  A+ IAN+     + +    +++ ++GESE  +  VF  A       ++F DE+DS A AR   + +   A  R+++ +L ++DG    K V +I ATNR   +DPA++   R D +I   LPD  +R +I   + +                    SG D+ ++CQ+A +    + I  +I ++ E+
Sbjct:  506 EKYLKFGMQPSRGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVRDVFDKARAAA-PCVLFFDELDSIAKARGGSVGDAGGAADRVINQVLTEMDGMNAKKNVFIIGATNRPDIIDPAVLRPGRLDQLIYIPLPDEASRLQIFKASLRKTPLSADLDLNFLAKNTVGFSGADLTEICQRACKLAIRESIEREIRQEKER 718          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: SPAST_IXOSC (Spastin OS=Ixodes scapularis GN=spas PE=3 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 3.361e-23
Identity = 57/141 (40.43%), Postives = 85/141 (60.28%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFE--QDKKVVVIAATNRKQDLDPALISRFDSMITFGLPDHENR 491
            P+ +L  GPPG GKT  A+ +A+++    + +    + SKY GE E+L+  +F++A EL   +IIF+DEVDS    R    HEATRR+ +  L + DG     +++V+V+ ATNR Q+LD A + RF   +   LPDH  R
Sbjct:  407 PKGLLLFGPPGNGKTMLAKAVAHESNSTFLNISAASLTSKYVGEGEKLVRALFAVAREL-QPSIIFIDEVDSLLSERKDNEHEATRRLKTEFLVEFDGLHTGSEERVLVMGATNRPQELDDAALRRFTKRVYVTLPDHNTR 546          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS6B_DICDI (26S protease regulatory subunit 6B homolog OS=Dictyostelium discoideum GN=psmC4 PE=1 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 3.361e-23
Identity = 71/185 (38.38%), Postives = 94/185 (50.81%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQ---EIAAQYAKHXXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PR VL  GPPGTGKT  A+ +A+      + V     + KY GE  RL+  VF LA E  + AIIF+DE+D+ A  R      A R   RIL  LL Q+DGF+    V VI ATNR+  LDPAL+   R D  I F LPD   ++   ++                      +SG +I+ +CQ+A
Sbjct:  184 PRGVLLYGPPGTGKTMLAKAVAHHTSAAFIRVVGSEFVQKYLGEGPRLVRDVFRLARE-NSPAIIFIDEIDAIATKRFDAQTGADREVQRILMELLNQMDGFDVSVNVKVIMATNRQDTLDPALLRPGRLDRKIEFPLPDRRQKRLIFQVITSKMNLSDEVDLEDYVSRPDKLSGAEIQSICQEA 367          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS6B_CAEEL (Probable 26S protease regulatory subunit 6B OS=Caenorhabditis elegans GN=rpt-3 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 3.361e-23
Identity = 78/223 (34.98%), Postives = 106/223 (47.53%), Query Frame = 3
Query:   21 EVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQAERSWASKIIRGQITKDGEQA 665
            E+Y  I ++         PR VL  GPPG GKT  A+ +A       + V     + KY GE  R++  VF LA E  + +IIF+DE+D+ A  R      A R   RIL  LL Q+DGF+Q   V VI ATNR+  LDPAL+   R D  I F LPD   ++ + +                       +SG DI  +CQ+A      +     +TKD E+A
Sbjct:  185 ELYQQIGIDP--------PRGVLMYGPPGCGKTMLAKAVAANTAASFIRVVGSEFVQKYLGEGPRMVRDVFRLAKE-NSPSIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQSTNVKVIMATNRQDTLDPALLRPGRLDRKIEFPLPDRRQKRLVFSTVCSRMNLSDDVDLEDWVARPDKISGADINSICQEAGMQAVRENRYVVLTKDLEKA 398          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: CD48E_ARATH (Cell division control protein 48 homolog E OS=Arabidopsis thaliana GN=CDC48E PE=2 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 3.361e-23
Identity = 64/151 (42.38%), Postives = 85/151 (56.29%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEIAAQYAKH 521
            P+ +L  GPPG+GKT  AR +AN+ G     +    +MSK  GESE  L K F  A E    +IIF+DE+DS A  R+    E  RRI+S LL  +DG +    V+V+ ATNR   +DPAL    RFD  I  G+PD   R E+   + K+
Sbjct:  241 PKGILLYGPPGSGKTLIARAVANETGAFFFCINGPEIMSKLAGESESNLRKAFEEA-EKNAPSIIFIDEIDSIAPKREKTNGEVERRIVSQLLTLMDGLKSRAHVIVMGATNRPNSIDPALRRFGRFDREIDIGVPDEIGRLEVLRIHTKN 390          

HSP 2 Score: 95.5153 bits (236), Expect = 2.945e-19
Identity = 68/209 (32.54%), Postives = 102/209 (48.80%), Query Frame = 3
Query:   57 KFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHE---ATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI---AAQYAKHXXXXXXXXXXXXXXXMSGRDIRDVCQQAERSWASKIIRGQITKDGE 659
            KF  +  + VLF GPPG GKT  A+ IAN+     + V    +++ ++GESE  + ++F  A +     ++F DE+DS A  R +   +   A  R+L+ LL ++DG    K V +I ATNR   +D AL+   R D +I   LPD ++R  I     + +                  SG DI ++CQ+A        IR  I KD E
Sbjct:  508 KFGMSPSKGVLFYGPPGCGKTLLAKAIANECQANFISVKGPELLTMWFGESEANVREIFDKARQ-SAPCVLFFDELDSIATQRGNSAGDAGGAADRVLNQLLTEMDGMNAKKTVFIIGATNRPDIIDSALLRPGRLDQLIYIPLPDEDSRLNIFKACLRKSPVAKDVDVTALAKYTQGFSGADITEICQRA----CKYAIRENIEKDIE 711          
The following BLAST results are available for this feature:
BLAST of CN188561 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 381
Match NameE-valueIdentityDescription
PS10A_ARATH1.155e-2339.7826S protease regulatory subunit 10B homolog A OS=A... [more]
CDC48_SOYBN1.155e-2343.05Cell division cycle protein 48 homolog OS=Glycine ... [more]
PSMR_METKA1.971e-2335.75Proteasome-activating nucleotidase OS=Methanopyrus... [more]
FIGL1_CAEBR1.971e-2335.33Fidgetin-like protein 1 OS=Caenorhabditis briggsae... [more]
CDC48_CAPAN1.971e-2342.38Cell division cycle protein 48 homolog OS=Capsicum... [more]
TERA2_CAEEL2.574e-2343.71Transitional endoplasmic reticulum ATPase homolog ... [more]
SPAST_IXOSC3.361e-2340.43Spastin OS=Ixodes scapularis GN=spas PE=3 SV=1[more]
PRS6B_DICDI3.361e-2338.3826S protease regulatory subunit 6B homolog OS=Dict... [more]
PRS6B_CAEEL3.361e-2334.98Probable 26S protease regulatory subunit 6B OS=Cae... [more]
CD48E_ARATH3.361e-2342.38Cell division control protein 48 homolog E OS=Arab... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006E21_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006E21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188561 ID=CN188561; Name=CN188561; organism=Citrus sinensis; type=EST; length=707bp
ACATTTTAGCTTCAAGCCCTGAAGTATATGATGATATTGCCCTTGAAACT
CGGTGTAAGTTTGAGTCGAATAGACCTAGGGCTGTGCTCTTTGAAGGTCC
ACCAGGTACAGGGAAGACATCATGTGCTCGTGTTATTGCTAATCAAGCGG
GTGTTCCATTGATGTATGTGCCACTGGAGGTTGTCATGTCAAAATACTAC
GGTGAAAGTGAGCGGCTATTGGGGAAAGTGTTTTCTCTTGCCAATGAGCT
TCCAAACGGAGCTATTATTTTCCTGGACGAGGTTGATTCTTTTGCTGTTG
CTCGTGATAGTGAAATGCATGAAGCTACCCGAAGAATTTTGTCCGTGTTG
TTGAGACAGATTGATGGATTCGAACAGGATAAAAAGGTGGTGGTGATTGC
TGCAACAAATAGAAAGCAAGACCTTGATCCTGCTTTAATAAGTCGGTTTG
ATTCAATGATTACCTTTGGCCTACCGGATCATGAGAATCGTCAGGAAATA
GCAGCTCAGTATGCAAAGCACCTAACAAAAGCTGAACTGGCCGAATTAGC
CACTGCTACAGAGGAAATGTCGGGAAGAGATATTAGGGATGTGTGTCAAC
AAGCAGAGCGATCATGGGCATCAAAGATAATTCGAGGGCAAATCACTAAG
GATGGAGAACAGGCTTGTCTTCCACCTCTACAAGAATACATTGAGAGTGC
TACAAAC
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