CN188561

Overview
NameCN188561
Unique NameCN188561
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length707
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS6B_RAT (26S protease regulatory subunit 6B OS=Rattus norvegicus GN=Psmc4 PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.854e-22
Identity = 71/185 (38.38%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PR VL  GPPG GKT  A+ +A+      + V     + KY GE  R++  VF LA E    AIIF+DE+D+ A  R      A R   RIL  LL Q+DGF+Q+  V VI ATNR   LDPAL+   R D  I F LPD   ++ I +                       +SG DI  +CQ++
Sbjct:  199 PRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKE-NAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQES 382          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS6B_MACFA (26S protease regulatory subunit 6B OS=Macaca fascicularis GN=PSMC4 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.854e-22
Identity = 71/185 (38.38%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PR VL  GPPG GKT  A+ +A+      + V     + KY GE  R++  VF LA E    AIIF+DE+D+ A  R      A R   RIL  LL Q+DGF+Q+  V VI ATNR   LDPAL+   R D  I F LPD   ++ I +                       +SG DI  +CQ++
Sbjct:  199 PRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKE-NAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQES 382          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS6B_HUMAN (26S protease regulatory subunit 6B OS=Homo sapiens GN=PSMC4 PE=1 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 4.854e-22
Identity = 71/185 (38.38%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PR VL  GPPG GKT  A+ +A+      + V     + KY GE  R++  VF LA E    AIIF+DE+D+ A  R      A R   RIL  LL Q+DGF+Q+  V VI ATNR   LDPAL+   R D  I F LPD   ++ I +                       +SG DI  +CQ++
Sbjct:  199 PRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKE-NAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQES 382          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PRS6B_BOVIN (26S protease regulatory subunit 6B OS=Bos taurus GN=PSMC4 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.854e-22
Identity = 71/185 (38.38%), Postives = 92/185 (49.73%), Query Frame = 3
Query:   75 PRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            PR VL  GPPG GKT  A+ +A+      + V     + KY GE  R++  VF LA E    AIIF+DE+D+ A  R      A R   RIL  LL Q+DGF+Q+  V VI ATNR   LDPAL+   R D  I F LPD   ++ I +                       +SG DI  +CQ++
Sbjct:  199 PRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLGEGPRMVRDVFRLAKE-NAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMATNRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQES 382          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: ATAD2_PONAB (ATPase family AAA domain-containing protein 2 OS=Pongo abelii GN=ATAD2 PE=2 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.854e-22
Identity = 69/168 (41.07%), Postives = 90/168 (53.57%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEV-----VMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEI 500
            PEV++        KF+   PR  LF GPPGTGKT  AR +AN+       V   +      +SK+ GESER L  +F  A ++   +IIF DE+D  A  R S   +    I+S LL  +DG +   ++VVI ATNR   +DPAL    RFD    F LPD E R+EI
Sbjct:  280 PEVFE--------KFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKRVAFFMRKGADCLSKWVGESERQLRLLFDQAYQM-RPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDSRGEIVVIGATNRLDAIDPALRRPGRFDREFLFSLPDKEARKEI 438          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: ATAD2_MOUSE (ATPase family AAA domain-containing protein 2 OS=Mus musculus GN=Atad2 PE=1 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 4.854e-22
Identity = 69/168 (41.07%), Postives = 88/168 (52.38%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAG-----VPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEI 500
            PEV++        KF+   PR  LF GPPGTGKT  AR +AN+       V          +SK+ GESER L  +F  A ++   AIIF DE+D  A  R S   +    I+S LL  +DG +   ++VVI ATNR   +DPAL    RFD    F LPD   R+EI
Sbjct:  104 PEVFE--------KFKIQPPRGCLFYGPPGTGKTLVARALANECSRGDKRVAFFMRKGADCLSKWVGESERQLRLLFDQAYQM-RPAIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDSRGEIVVIGATNRLDSIDPALRRPGRFDREFLFSLPDKNARKEI 262          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: YB7A_SCHPO (Uncharacterized AAA domain-containing protein C16E9.10c OS=Schizosaccharomyces pombe GN=SPBC16E9.10c PE=1 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 8.279e-22
Identity = 60/169 (35.50%), Postives = 89/169 (52.66%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAK 518
            PE+Y  + +          P  VL  GPPG GKT  A+ +AN++    + +    +++KY GESER + +VF L     +  +IF DE+D+    RD  + EA+ R+++ LL ++DG      V VIAATNR   +DPA++   R D  +   LPD   R EI     K
Sbjct:  515 PELYQSVGISA--------PTGVLLWGPPGCGKTLLAKAVANESKANFISIRGPELLNKYVGESERAVRQVF-LRARASSPCVIFFDELDAMVPRRDDSLSEASSRVVNTLLTELDGLSDRSGVYVIAATNRPDIIDPAMLRPGRLDKTLLVDLPDAHERVEILKTLTK 674          

HSP 2 Score: 97.0561 bits (240), Expect = 1.012e-19
Identity = 65/173 (37.57%), Postives = 90/173 (52.02%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATRRILSVLLRQID--GFEQ--DKKVVVIAATNRKQDLDPAL--ISRFDSMITFGLPDHENRQEIAAQYAK 518
            PEVY    +          PR VL  GPPG GKT  A  +AN+ GVP + +    ++S   GESE+ + +VF  A  L    ++F+DE+D+    R+S   E  RRI++  L  +D   FE+   K V+VI ATNR   LD AL    RFD  I   +P  + R++I    AK
Sbjct:  197 PEVYQYTGIHP--------PRGVLLHGPPGCGKTMLANALANELGVPFISISAPSIVSGMSGESEKKVREVFEEAKSLA-PCLMFIDEIDAVTPKRESAQREMERRIVAQFLTCMDELSFEKTDGKPVLVIGATNRPDSLDSALRRAGRFDREICLTVPSQDAREKILRTMAK 360          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_SULSO (Proteasome-activating nucleotidase OS=Sulfolobus solfataricus GN=pan PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 8.279e-22
Identity = 69/205 (33.66%), Postives = 103/205 (50.24%), Query Frame = 3
Query:   15 SPEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVAR---DSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQYAKH---XXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            +PE++ +I +E         P+ VL  GPPGTGKT  A+ +A ++    ++V       K+ GE  R++ ++F +A      +IIF+DE+D+    R    +      +R L  LL ++DGF+    V +IAATNR   LDPAL+   RFD +I   LPD + R EI   Y K                    SG DI++VC +A
Sbjct:  156 NPEIFREIGVEP--------PKGVLLYGPPGTGKTMLAKAVATESNAVFIHVVASEFAQKFVGEGARIVRELFEMAKR-KAPSIIFIDEIDAIGAKRIDIGTSGEREIQRTLMQLLAELDGFDPLDNVKIIAATNRIDILDPALLRPGRFDRIIEVPLPDFKGRTEIFNIYLKKMKIEDNINLELLSQLTEGFSGADIKNVCVEA 351          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_SULTO (Proteasome-activating nucleotidase OS=Sulfolobus tokodaii GN=pan PE=3 SV=2)

HSP 1 Score: 103.605 bits (257), Expect = 1.081e-21
Identity = 69/205 (33.66%), Postives = 103/205 (50.24%), Query Frame = 3
Query:   15 SPEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVAR---DSEMHEATRRILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEIAAQY---AKHXXXXXXXXXXXXXXXMSGRDIRDVCQQA 605
            +PE++ +I ++         P+ VL  GPPGTGKT  A+ +A ++    + V       K+ GE  R++ +VF LA      +I+F+DE+D+    R    +      +R L  LL +IDGF+    V +IAATNR   LDPAL+   RFD +I   LP+ E R+EI   Y    K                 SG +I++VC +A
Sbjct:  156 NPELFKEIGIDP--------PKGVLLYGPPGTGKTLLAKAVATESNATFIQVVASEFAQKFVGEGARIVREVFELARR-KAPSIVFIDEIDAIGAKRVDMGTSGEREIQRTLMQLLAEIDGFKPLDNVKIIAATNRLDILDPALLRPGRFDRLIEVPLPNFEGRKEIFRIYLQKMKTDGNIRYDILASMTEGFSGAEIKNVCTEA 351          
BLAST of CN188561 vs. ExPASy Swiss-Prot
Match: PSMR_PYRKO (Proteasome-activating nucleotidase OS=Pyrococcus kodakaraensis GN=pan PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.081e-21
Identity = 65/166 (39.16%), Postives = 90/166 (54.22%), Query Frame = 3
Query:   18 PEVYDDIALETRCKFESNRPRAVLFEGPPGTGKTSCARVIANQAGVPLMYVPLEVVMSKYYGESERLLGKVFSLANELPNGAIIFLDEVDSFAVARDSEMHEATR---RILSVLLRQIDGFEQDKKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDHENRQEI 500
            PE+++ + +E         P+ VL  GPPG GKT  A+ +AN      + V    ++ K+ GE  RL+ ++F LA E     IIF+DE+D+    R  E     R   R L  LL ++DGF+    V VIAATNR   LDPAL+   RFD +I   LPD++ R EI
Sbjct:  161 PELFEQVGIEP--------PKGVLLYGPPGCGKTLMAKAVANHVNATFIRVVGSELVRKFIGEGARLVHELFELAKE-KAPTIIFIDEIDAIGAKRMDETTGGEREVNRTLMQLLAEMDGFDPRGNVKVIAATNRPDILDPALLRPGRFDRLIEVPLPDYQGRLEI 317          
The following BLAST results are available for this feature:
BLAST of CN188561 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 381
Match NameE-valueIdentityDescription
PRS6B_RAT4.854e-2238.3826S protease regulatory subunit 6B OS=Rattus norve... [more]
PRS6B_MACFA4.854e-2238.3826S protease regulatory subunit 6B OS=Macaca fasci... [more]
PRS6B_HUMAN4.854e-2238.3826S protease regulatory subunit 6B OS=Homo sapiens... [more]
PRS6B_BOVIN4.854e-2238.3826S protease regulatory subunit 6B OS=Bos taurus G... [more]
ATAD2_PONAB4.854e-2241.07ATPase family AAA domain-containing protein 2 OS=P... [more]
ATAD2_MOUSE4.854e-2241.07ATPase family AAA domain-containing protein 2 OS=M... [more]
YB7A_SCHPO8.279e-2235.50Uncharacterized AAA domain-containing protein C16E... [more]
PSMR_SULSO8.279e-2233.66Proteasome-activating nucleotidase OS=Sulfolobus s... [more]
PSMR_SULTO1.081e-2133.66Proteasome-activating nucleotidase OS=Sulfolobus t... [more]
PSMR_PYRKO1.081e-2139.16Proteasome-activating nucleotidase OS=Pyrococcus k... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006E21_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006E21, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188561 ID=CN188561; Name=CN188561; organism=Citrus sinensis; type=EST; length=707bp
ACATTTTAGCTTCAAGCCCTGAAGTATATGATGATATTGCCCTTGAAACT
CGGTGTAAGTTTGAGTCGAATAGACCTAGGGCTGTGCTCTTTGAAGGTCC
ACCAGGTACAGGGAAGACATCATGTGCTCGTGTTATTGCTAATCAAGCGG
GTGTTCCATTGATGTATGTGCCACTGGAGGTTGTCATGTCAAAATACTAC
GGTGAAAGTGAGCGGCTATTGGGGAAAGTGTTTTCTCTTGCCAATGAGCT
TCCAAACGGAGCTATTATTTTCCTGGACGAGGTTGATTCTTTTGCTGTTG
CTCGTGATAGTGAAATGCATGAAGCTACCCGAAGAATTTTGTCCGTGTTG
TTGAGACAGATTGATGGATTCGAACAGGATAAAAAGGTGGTGGTGATTGC
TGCAACAAATAGAAAGCAAGACCTTGATCCTGCTTTAATAAGTCGGTTTG
ATTCAATGATTACCTTTGGCCTACCGGATCATGAGAATCGTCAGGAAATA
GCAGCTCAGTATGCAAAGCACCTAACAAAAGCTGAACTGGCCGAATTAGC
CACTGCTACAGAGGAAATGTCGGGAAGAGATATTAGGGATGTGTGTCAAC
AAGCAGAGCGATCATGGGCATCAAAGATAATTCGAGGGCAAATCACTAAG
GATGGAGAACAGGCTTGTCTTCCACCTCTACAAGAATACATTGAGAGTGC
TACAAAC
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