CN188450

Overview
NameCN188450
Unique NameCN188450
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length820
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13665 contig Csv1_Contig13665:67..883. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_SOLTU (ADP-ribosylation factor 1 OS=Solanum tuberosum PE=2 SV=2)

HSP 1 Score: 327.02 bits (837), Expect = 2.652e-90
Identity = 161/172 (93.60%), Postives = 165/172 (95.93%), Query Frame = -3 
Query:  249 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANKA*SC 764
            FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDR+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSN I N+  +C
Sbjct:   13 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNQIRNQKANC 184

HSP 2 Score: 26.5646 bits (57), Expect = 2.652e-90
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = -2 
Query:  754 MGLTFTKLFSRLFCQE 801
            MGLT +KLFSRLF ++
Sbjct:    1 MGLTISKLFSRLFAKK 16
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_XENLA (ADP-ribosylation factor 1 OS=Xenopus laevis GN=arf1 PE=2 SV=2)

HSP 1 Score: 315.849 bits (808), Expect = 1.769e-85
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -3 
Query:  261 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ 180
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_RAT (ADP-ribosylation factor 1 OS=Rattus norvegicus GN=Arf1 PE=1 SV=2)

HSP 1 Score: 315.849 bits (808), Expect = 1.769e-85
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -3 
Query:  261 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ 180
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_MOUSE (ADP-ribosylation factor 1 OS=Mus musculus GN=Arf1 PE=1 SV=2)

HSP 1 Score: 315.849 bits (808), Expect = 1.769e-85
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -3 
Query:  261 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ 180
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_MACFA (ADP-ribosylation factor 1 OS=Macaca fascicularis GN=ARF1 PE=2 SV=3)

HSP 1 Score: 315.849 bits (808), Expect = 1.769e-85
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -3 
Query:  261 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ 180
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_HUMAN (ADP-ribosylation factor 1 OS=Homo sapiens GN=ARF1 PE=1 SV=2)

HSP 1 Score: 315.849 bits (808), Expect = 1.769e-85
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -3 
Query:  261 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ 180
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_BOVIN (ADP-ribosylation factor 1 OS=Bos taurus GN=ARF1 PE=1 SV=2)

HSP 1 Score: 315.849 bits (808), Expect = 1.769e-85
Identity = 154/170 (90.59%), Postives = 162/170 (95.29%), Query Frame = -3 
Query:  261 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQ 180
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_LOCMI (ADP-ribosylation factor 1 OS=Locusta migratoria GN=ARF1 PE=2 SV=2)

HSP 1 Score: 315.464 bits (807), Expect = 2.310e-85
Identity = 154/169 (91.12%), Postives = 161/169 (95.27%), Query Frame = -3 
Query:  264 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAN 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI EAR+EL RML EDELRDAVLL+FANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIGEAREELMRMLAEDELRDAVLLIFANKQDLPNAMNAAEITDKLGLHSLRNRNWYIQATCATSGDGLYEGLDWLSNQLKN 179
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF1_DROME (ADP-ribosylation factor 1 OS=Drosophila melanogaster GN=Arf79F PE=1 SV=2)

HSP 1 Score: 315.464 bits (807), Expect = 2.310e-85
Identity = 154/169 (91.12%), Postives = 161/169 (95.27%), Query Frame = -3 
Query:  264 GSFAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAN 770
            G F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI EAR+EL RML EDELRDAVLL+FANKQDLPNAMNAAEITDKLGLHSLR R+WYIQ+TCATSG+GLYEGLDWLSN + N
Sbjct:   11 GLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIGEAREELMRMLAEDELRDAVLLIFANKQDLPNAMNAAEITDKLGLHSLRNRNWYIQATCATSGDGLYEGLDWLSNQLKN 179
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF2_RAT (ADP-ribosylation factor 2 OS=Rattus norvegicus GN=Arf2 PE=2 SV=1)

HSP 1 Score: 314.694 bits (805), Expect = 3.941e-85
Identity = 153/168 (91.07%), Postives = 161/168 (95.83%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ EAR+EL RML EDELRDAVLLVF NKQDLPNAMNAAEITDKLGLHSLRQR+WYIQ+TCATSG+GLYEGLDWLSN + N+
Sbjct:   13 FGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELTRMLAEDELRDAVLLVFVNKQDLPNAMNAAEITDKLGLHSLRQRNWYIQATCATSGDGLYEGLDWLSNQLKNQ 180
The following BLAST results are available for this feature:
BLAST of CN188450 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARF1_SOLTU2.652e-9093.60ADP-ribosylation factor 1 OS=Solanum tuberosum PE=... [more]
ARF1_XENLA1.769e-8590.59ADP-ribosylation factor 1 OS=Xenopus laevis GN=arf... [more]
ARF1_RAT1.769e-8590.59ADP-ribosylation factor 1 OS=Rattus norvegicus GN=... [more]
ARF1_MOUSE1.769e-8590.59ADP-ribosylation factor 1 OS=Mus musculus GN=Arf1 ... [more]
ARF1_MACFA1.769e-8590.59ADP-ribosylation factor 1 OS=Macaca fascicularis G... [more]
ARF1_HUMAN1.769e-8590.59ADP-ribosylation factor 1 OS=Homo sapiens GN=ARF1 ... [more]
ARF1_BOVIN1.769e-8590.59ADP-ribosylation factor 1 OS=Bos taurus GN=ARF1 PE... [more]
ARF1_LOCMI2.310e-8591.12ADP-ribosylation factor 1 OS=Locusta migratoria GN... [more]
ARF1_DROME2.310e-8591.12ADP-ribosylation factor 1 OS=Drosophila melanogast... [more]
ARF2_RAT3.941e-8591.07ADP-ribosylation factor 2 OS=Rattus norvegicus GN=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006A22_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006A22, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188450 ID=CN188450; Name=CN188450; organism=Citrus sinensis; type=EST; length=820bp
TTTTTGCGAAAACAATTTCAATGAGTGGTGCACCAAATTAGTTTAAATTA
CCGATATCTAAACCGAGCCAAAAACCTTGGAGTACATGTATCCAACCAAC
AATTCTTCTAGTACAATATAGTTGTAAAGATCCTAGGGCCAAAACAACAC
AACAACCCAACCGTCTCAAACAAAAAGGGTTAAAAAAGAAAAAAAATAGA
TAATATTCACCCCTGCATCCAGAATCGCCAGTCCGCAAAAAGATCTTAAC
AGCTTCAGGCCTTGTTAGCGATGTTGTTGGAGAGCCAATCCAGACCTTCA
TAAAGACCCTCACCAGAGGTTGCACAGGTGCTCTGGATGTACCAGTGGCG
CTGCCTGAGGGAGTGGAGGCCAAGCTTATCAGTAATCTCAGCAGCATTCA
TTGCATTTGGAAGATCCTGTTTGTTAGCAAATACAAGCAACACTGCATCT
CGCAGCTCATCCTCATTCAACATCCTATGCAATTCATCCCTTGCTTCTAC
AATACGGTCTCTGTCATTGCTGTCCACAACAAAAATGAGACCTTGAGTGT
TCTGGAAATAATGCCTCCACAAGGGACGAATCTTGTCCTGGCCACCAACA
TCCCACACAGTGAAACTGATGTTCTTATATTCAACGGTCTCCACATTAAA
CCCAATGGTAGGAATTGTGGTGACAATCTCTCCAAGCTTGAGCTTGTACA
AGATAGTGGTCTTACCAGCAGCATCAAGACCAACCATTAGAATTCGCATC
TCTTTCTTGGCAAAAGAGCCGGCTGAACAGCTTTGTGAAAGTCAGCCCCA
TTCTTCCGGCTGGGGATCAC
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