CN188450

Overview
NameCN188450
Unique NameCN188450
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length820
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig13665 contig Csv1_Contig13665:67..883. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF4_XENLA (ADP-ribosylation factor 4 OS=Xenopus laevis GN=arf4 PE=2 SV=2)

HSP 1 Score: 290.041 bits (741), Expect = 2.051e-78
Identity = 140/168 (83.33%), Postives = 152/168 (90.48%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI EA +EL +ML EDELRDAVLLVFANKQDLPNAM  +E+TDKL L +LR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEAAEELQKMLQEDELRDAVLLVFANKQDLPNAMAISEMTDKLTLQTLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180

HSP 2 Score: 23.8682 bits (50), Expect = 2.051e-78
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSRLF
Sbjct:    1 MGLTISSLFSRLF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF5_RAT (ADP-ribosylation factor 5 OS=Rattus norvegicus GN=Arf5 PE=1 SV=2)

HSP 1 Score: 289.271 bits (739), Expect = 1.010e-77
Identity = 137/168 (81.55%), Postives = 152/168 (90.48%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ E+ DEL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL  LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180

HSP 2 Score: 22.3274 bits (46), Expect = 1.010e-77
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSR+F
Sbjct:    1 MGLTVSALFSRIF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF5_MOUSE (ADP-ribosylation factor 5 OS=Mus musculus GN=Arf5 PE=2 SV=2)

HSP 1 Score: 289.271 bits (739), Expect = 1.010e-77
Identity = 137/168 (81.55%), Postives = 152/168 (90.48%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ E+ DEL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL  LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180

HSP 2 Score: 22.3274 bits (46), Expect = 1.010e-77
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSR+F
Sbjct:    1 MGLTVSALFSRIF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF5_HUMAN (ADP-ribosylation factor 5 OS=Homo sapiens GN=ARF5 PE=1 SV=2)

HSP 1 Score: 289.271 bits (739), Expect = 1.010e-77
Identity = 137/168 (81.55%), Postives = 152/168 (90.48%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ E+ DEL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL  LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180

HSP 2 Score: 22.3274 bits (46), Expect = 1.010e-77
Identity = 9/13 (69.23%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSR+F
Sbjct:    1 MGLTVSALFSRIF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF4_PONAB (ADP-ribosylation factor 4 OS=Pongo abelii GN=ARF4 PE=2 SV=3)

HSP 1 Score: 286.96 bits (733), Expect = 1.718e-77
Identity = 138/168 (82.14%), Postives = 151/168 (89.88%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQD+IRPLW+HYFQNTQGLIFVVDSNDR+RI E  DEL +ML  DELRDAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180

HSP 2 Score: 23.8682 bits (50), Expect = 1.718e-77
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSRLF
Sbjct:    1 MGLTISSLFSRLF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF4_HUMAN (ADP-ribosylation factor 4 OS=Homo sapiens GN=ARF4 PE=1 SV=3)

HSP 1 Score: 286.96 bits (733), Expect = 1.718e-77
Identity = 138/168 (82.14%), Postives = 151/168 (89.88%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQD+IRPLW+HYFQNTQGLIFVVDSNDR+RI E  DEL +ML  DELRDAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180

HSP 2 Score: 23.8682 bits (50), Expect = 1.718e-77
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSRLF
Sbjct:    1 MGLTISSLFSRLF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF2_YEAST (ADP-ribosylation factor 2 OS=Saccharomyces cerevisiae GN=ARF2 PE=1 SV=3)

HSP 1 Score: 288.886 bits (738), Expect = 2.316e-77
Identity = 135/169 (79.88%), Postives = 156/169 (92.31%), Query Frame = -3 
Query:  258 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANKA 764
            F  KEMRILMVGLD AGKTT+LYKLKLGE++TTIPTIGFNVETV+YKNISFTVWDVGGQD+IR LWRHY++NT+G+IFV+DSNDR RI EAR+ + RMLNEDELR+AV LVFANKQDLP AM+AAEIT+KLGLHS+R R W+IQSTCATSGEGLYEGL+WLSNN+ N++
Sbjct:   13 FGNKEMRILMVGLDGAGKTTVLYKLKLGEVITTIPTIGFNVETVQYKNISFTVWDVGGQDRIRSLWRHYYRNTEGVIFVIDSNDRSRIGEAREVMQRMLNEDELRNAVWLVFANKQDLPEAMSAAEITEKLGLHSIRNRPWFIQSTCATSGEGLYEGLEWLSNNLKNQS 181
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF5_CHICK (ADP-ribosylation factor 5 OS=Gallus gallus GN=ARF5 PE=2 SV=2)

HSP 1 Score: 288.115 bits (736), Expect = 3.811e-77
Identity = 136/168 (80.95%), Postives = 153/168 (91.07%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+R+ E+ +EL +ML EDELRDAVLLVFANKQD+PNAM  +E+TDKLGL +LR R WY+Q+TCAT G GLY+GLDWLS+ ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESAEELQKMLQEDELRDAVLLVFANKQDMPNAMVVSELTDKLGLQALRSRTWYVQATCATQGTGLYDGLDWLSHELSKR 180

HSP 2 Score: 21.557 bits (44), Expect = 3.811e-77
Identity = 8/13 (61.54%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + +FSR+F
Sbjct:    1 MGLTVSAIFSRIF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF4_RAT (ADP-ribosylation factor 4 OS=Rattus norvegicus GN=Arf4 PE=2 SV=2)

HSP 1 Score: 284.648 bits (727), Expect = 8.456e-77
Identity = 138/168 (82.14%), Postives = 150/168 (89.29%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI E    L +ML EDEL+DAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEGAAVLQKMLLEDELQDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180

HSP 2 Score: 23.8682 bits (50), Expect = 8.456e-77
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSRLF
Sbjct:    1 MGLTISSLFSRLF 13
[Close]
BLAST of CN188450 vs. ExPASy Swiss-Prot
Match: ARF4_MOUSE (ADP-ribosylation factor 4 OS=Mus musculus GN=Arf4 PE=2 SV=2)

HSP 1 Score: 284.648 bits (727), Expect = 8.456e-77
Identity = 138/168 (82.14%), Postives = 150/168 (89.29%), Query Frame = -3 
Query:  261 FAKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRIVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIANK 764
            F KK+MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNI FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDR+RI E    L +ML EDEL+DAVLL+FANKQDLPNAM  +E+TDKLGL SLR R WY+Q+TCAT G GLYEGLDWLSN ++ +
Sbjct:   13 FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEGAAVLQKMLLEDELQDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR 180

HSP 2 Score: 23.8682 bits (50), Expect = 8.456e-77
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = -2 
Query:  763 MGLTFTKLFSRLF 801
            MGLT + LFSRLF
Sbjct:    1 MGLTISSLFSRLF 13
The following BLAST results are available for this feature:
BLAST of CN188450 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 217
Match NameE-valueIdentityDescription
ARF4_XENLA2.051e-7883.33ADP-ribosylation factor 4 OS=Xenopus laevis GN=arf... [more]
ARF5_RAT1.010e-7781.55ADP-ribosylation factor 5 OS=Rattus norvegicus GN=... [more]
ARF5_MOUSE1.010e-7781.55ADP-ribosylation factor 5 OS=Mus musculus GN=Arf5 ... [more]
ARF5_HUMAN1.010e-7781.55ADP-ribosylation factor 5 OS=Homo sapiens GN=ARF5 ... [more]
ARF4_PONAB1.718e-7782.14ADP-ribosylation factor 4 OS=Pongo abelii GN=ARF4 ... [more]
ARF4_HUMAN1.718e-7782.14ADP-ribosylation factor 4 OS=Homo sapiens GN=ARF4 ... [more]
ARF2_YEAST2.316e-7779.88ADP-ribosylation factor 2 OS=Saccharomyces cerevis... [more]
ARF5_CHICK3.811e-7780.95ADP-ribosylation factor 5 OS=Gallus gallus GN=ARF5... [more]
ARF4_RAT8.456e-7782.14ADP-ribosylation factor 4 OS=Rattus norvegicus GN=... [more]
ARF4_MOUSE8.456e-7782.14ADP-ribosylation factor 4 OS=Mus musculus GN=Arf4 ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0006A22_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0006A22, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188450 ID=CN188450; Name=CN188450; organism=Citrus sinensis; type=EST; length=820bp
TTTTTGCGAAAACAATTTCAATGAGTGGTGCACCAAATTAGTTTAAATTA
CCGATATCTAAACCGAGCCAAAAACCTTGGAGTACATGTATCCAACCAAC
AATTCTTCTAGTACAATATAGTTGTAAAGATCCTAGGGCCAAAACAACAC
AACAACCCAACCGTCTCAAACAAAAAGGGTTAAAAAAGAAAAAAAATAGA
TAATATTCACCCCTGCATCCAGAATCGCCAGTCCGCAAAAAGATCTTAAC
AGCTTCAGGCCTTGTTAGCGATGTTGTTGGAGAGCCAATCCAGACCTTCA
TAAAGACCCTCACCAGAGGTTGCACAGGTGCTCTGGATGTACCAGTGGCG
CTGCCTGAGGGAGTGGAGGCCAAGCTTATCAGTAATCTCAGCAGCATTCA
TTGCATTTGGAAGATCCTGTTTGTTAGCAAATACAAGCAACACTGCATCT
CGCAGCTCATCCTCATTCAACATCCTATGCAATTCATCCCTTGCTTCTAC
AATACGGTCTCTGTCATTGCTGTCCACAACAAAAATGAGACCTTGAGTGT
TCTGGAAATAATGCCTCCACAAGGGACGAATCTTGTCCTGGCCACCAACA
TCCCACACAGTGAAACTGATGTTCTTATATTCAACGGTCTCCACATTAAA
CCCAATGGTAGGAATTGTGGTGACAATCTCTCCAAGCTTGAGCTTGTACA
AGATAGTGGTCTTACCAGCAGCATCAAGACCAACCATTAGAATTCGCATC
TCTTTCTTGGCAAAAGAGCCGGCTGAACAGCTTTGTGAAAGTCAGCCCCA
TTCTTCCGGCTGGGGATCAC
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