CN188363

Overview
NameCN188363
Unique NameCN188363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length809
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: UBIQ_LEIMA (Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 2.629e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 142.51 bits (358), Expect = 2.629e-33
Identity = 71/76 (93.42%), Postives = 74/76 (97.37%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            MQIFVKTLTGKTI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGKTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 88.5817 bits (218), Expect = 4.506e-17
Identity = 42/44 (95.45%), Postives = 44/44 (100.00%), Query Frame = -2 
Query:  677 KEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 808
            KEGIPPDQQRLIFAGKQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   33 KEGIPPDQQRLIFAGKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: UBIQ_LEITA (Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 8.458e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 2 Score: 137.502 bits (345), Expect = 8.458e-32
Identity = 69/76 (90.79%), Postives = 72/76 (94.74%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            MQIFVKTLTG TI LEVE SDTI+NVKAKIQDKEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:    1 MQIFVKTLTGTTIALEVEPSDTIENVKAKIQDKEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76

HSP 3 Score: 85.8853 bits (211), Expect = 2.920e-16
Identity = 41/44 (93.18%), Postives = 43/44 (97.73%), Query Frame = -2 
Query:  677 KEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 808
            KEGIPPDQQRLIFA KQLE+GRTL+DYNIQKESTLHLVLRLRGG
Sbjct:   33 KEGIPPDQQRLIFADKQLEEGRTLSDYNIQKESTLHLVLRLRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: UBIL_NPVOP (Ubiquitin-like protein OS=Orgyia pseudotsugata multicapsid polyhedrosis virus GN=V-UBI PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 4.344e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 2 Score: 125.176 bits (313), Expect = 4.344e-28
Identity = 61/76 (80.26%), Postives = 67/76 (88.16%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            MQIFVKTLTGKTIT+E E  DT+  VK KI DKEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFVKTLTGKTITVETEPGDTVGQVKQKIADKEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 81.6481 bits (200), Expect = 5.508e-15
Identity = 38/44 (86.36%), Postives = 42/44 (95.45%), Query Frame = -2 
Query:  677 KEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 808
            KEG+P DQQRLI+AGKQLED +TLADYNIQKESTLH+VLRLRGG
Sbjct:   33 KEGVPVDQQRLIYAGKQLEDAQTLADYNIQKESTLHMVLRLRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: UBIL_NPVAC (Ubiquitin-like protein OS=Autographa californica nuclear polyhedrosis virus GN=V-UBI PE=3 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 1.264e-27
Identity = 58/77 (75.32%), Postives = 69/77 (89.61%), Query Frame = -2 
Query:  218 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 448
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG+
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGGY 77

HSP 2 Score: 122.479 bits (306), Expect = 2.816e-27
Identity = 58/76 (76.32%), Postives = 68/76 (89.47%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            MQIF+KTLTGKTIT E E ++T+ ++K KI DKEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:    1 MQIFIKTLTGKTITAETEPAETVADLKQKIADKEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76

HSP 3 Score: 82.4185 bits (202), Expect = 3.229e-15
Identity = 38/44 (86.36%), Postives = 42/44 (95.45%), Query Frame = -2 
Query:  677 KEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 808
            KEG+P DQQRLIFAGKQLED +T+ADYNIQKESTLH+VLRLRGG
Sbjct:   33 KEGVPVDQQRLIFAGKQLEDSKTMADYNIQKESTLHMVLRLRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: UBIQ_LUMTE (Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 1.070e-26
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = -2 
Query:  455 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 643
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 2 Score: 120.553 bits (301), Expect = 1.070e-26
Identity = 60/63 (95.24%), Postives = 62/63 (98.41%), Query Frame = -2 
Query:  227 TITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 415
            TITLEVE SDTI+NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:    1 TITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63

HSP 3 Score: 85.5001 bits (210), Expect = 3.814e-16
Identity = 41/42 (97.62%), Postives = 42/42 (100.00%), Query Frame = -2 
Query:  683 KEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLR 808
            KEGIPPDQQRLIFAGKQLEDGRTL+DYNIQKESTLHLVLRLR
Sbjct:   22 KEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLR 63
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB1_ARATH (NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN=RUB1 PE=1 SV=2)

HSP 1 Score: 101.293 bits (251), Expect = 6.717e-21
Identity = 48/75 (64.00%), Postives = 60/75 (80.00%), Query Frame = -2 
Query:  218 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 442
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGGF
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGF 77

HSP 2 Score: 98.9821 bits (245), Expect = 3.334e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = -2 
Query:  449 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 670
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB1_DESAN (NEDD8-like protein RUB1 OS=Deschampsia antarctica GN=RUB1 PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.146e-20
Identity = 48/75 (64.00%), Postives = 60/75 (80.00%), Query Frame = -2 
Query:  218 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 442
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGY 77

HSP 2 Score: 99.3673 bits (246), Expect = 2.552e-20
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = -2 
Query:  449 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 670
            I VKTLTGK I +++E +DTID VK ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRVKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB1_ORYSJ (NEDD8-like protein RUB1 OS=Oryza sativa subsp. japonica GN=RUB1 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.496e-20
Identity = 47/75 (62.67%), Postives = 60/75 (80.00%), Query Frame = -2 
Query:  218 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 442
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGGY 77

HSP 2 Score: 98.9821 bits (245), Expect = 3.334e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = -2 
Query:  449 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 670
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.552e-20
Identity = 47/77 (61.04%), Postives = 59/77 (76.62%), Query Frame = -2 
Query:  218 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGF 448
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGGF
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGGF 77

HSP 2 Score: 97.0561 bits (240), Expect = 1.267e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB2_ORYSJ (NEDD8-like protein RUB2 OS=Oryza sativa subsp. japonica GN=RUB2 PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.334e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = -2 
Query:  449 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 670
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76

HSP 2 Score: 98.9821 bits (245), Expect = 3.334e-20
Identity = 47/74 (63.51%), Postives = 59/74 (79.73%), Query Frame = -2 
Query:  221 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 442
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DYNI+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYNIEGGSVLHLVLALRGG 76
The following BLAST results are available for this feature:
BLAST of CN188363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
UBIQ_LEIMA2.629e-3393.42Ubiquitin OS=Leishmania major GN=UB-EP52 PE=3 SV=1[more]
UBIQ_LEITA8.458e-3290.79Ubiquitin OS=Leishmania tarentolae GN=UB-EP52 PE=3... [more]
UBIL_NPVOP4.344e-2880.26Ubiquitin-like protein OS=Orgyia pseudotsugata mul... [more]
UBIL_NPVAC1.264e-2775.32Ubiquitin-like protein OS=Autographa californica n... [more]
UBIQ_LUMTE1.070e-2695.24Ubiquitin (Fragment) OS=Lumbricus terrestris PE=1 ... [more]
RUB1_ARATH6.717e-2164.00NEDD8-like protein RUB1 OS=Arabidopsis thaliana GN... [more]
RUB1_DESAN1.146e-2064.00NEDD8-like protein RUB1 OS=Deschampsia antarctica ... [more]
RUB1_ORYSJ1.496e-2062.67NEDD8-like protein RUB1 OS=Oryza sativa subsp. jap... [more]
NEDD8_CAEEL2.552e-2061.04NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ORYSJ3.334e-2063.51NEDD8-like protein RUB2 OS=Oryza sativa subsp. jap... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005O14_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005O14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188363 ID=CN188363; Name=CN188363; organism=Citrus sinensis; type=EST; length=809bp
GAGGACACACACAAGACAATTTATTCATGAATCAGAAAACCATAGCCTTA
TTAGAGGCCATTTCAGAGTACGACAACAGATAACAAAAGCTTATTATAAG
CATACCATAAAACAACATTTTGCAGCTTAATCTAAGTCATTAAGGCCACA
AACGAAACAGAAAAAAGAAAGCAAACCCAAAGAACACAGACACTGGCAGT
GGACCAGAGTGCCATTAAAAACCACCACGCAGGCGAAGGACAAGATGTAA
AGTAGACTCCTTCTGGATATTGTAGTCAGCCAAAGTACGCCCATCCTCCA
GCTGCTTCCCGGCAAAAATCAACCTCTGCTGATCTGGAGGAATCCCTTCC
TTGTCCTGGATTTTAGCCTTCACATTATCAATGGTGTCTGAACTCTCAAC
CTCCAGGGTAATGGTTTTTCCCGTCAATGTCTTCACGAAAATCTGCATTC
CTCCACGCAGTCTCAACACCAAATGCAGTGTGGATTCCTTCTGAATGTTG
TAATCTGCAAGGGTCCTCCCATCTTCAAGCTGCTTGCCTGCAAAAATCAA
CCTCTGCTGGTCTGGTGGAATGCCCTCCTTATCTTGGATCTTGGCCTTCA
CATTATCAATAGTGTCCGAACTCTCTACCTCCAGAGTGATTGTTTTTCCA
GTGAGAGTCTTCACAAAAATTTGCATGCCACCACGAAGGCGGAGAACCAA
GTGAAGGGTGGACTCCTTCTGAATGTTATAATCTGCAAGGGTCCTACCAT
CTTCAAGCTGCTTGCCAGCAAAAATCAACCTCTGCTGGTCGGGAGGAATG
CCCTCCTTA
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