CN188363

Overview
NameCN188363
Unique NameCN188363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length809
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig2087 contig Csv1_Contig2087:66..874. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.334e-20
Identity = 46/79 (58.23%), Postives = 60/79 (75.95%), Query Frame = -2 
Query:  440 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 676
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79

HSP 2 Score: 98.5969 bits (244), Expect = 4.354e-20
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.654e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = -2 
Query:  446 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 670
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 95.9005 bits (237), Expect = 2.822e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -2 
Query:  221 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 442
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 1.829e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.389e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 92.8189 bits (229), Expect = 2.389e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.549e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  449 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 676
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 90.1225 bits (222), Expect = 1.549e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of CN188363 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.022e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = -2 
Query:  446 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 676
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 88.9669 bits (219), Expect = 3.450e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = -2 
Query:  221 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 448
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76
The following BLAST results are available for this feature:
BLAST of CN188363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
NEDD8_DROME3.334e-2058.23NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=... [more]
RUB2_ARATH1.654e-1961.33NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
NEDD8_RAT1.829e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.829e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.829e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.829e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.829e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ORYSJ2.389e-1853.95NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
RUB3_ARATH1.549e-1755.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI2.022e-1753.25NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005O14_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005O14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188363 ID=CN188363; Name=CN188363; organism=Citrus sinensis; type=EST; length=809bp
GAGGACACACACAAGACAATTTATTCATGAATCAGAAAACCATAGCCTTA
TTAGAGGCCATTTCAGAGTACGACAACAGATAACAAAAGCTTATTATAAG
CATACCATAAAACAACATTTTGCAGCTTAATCTAAGTCATTAAGGCCACA
AACGAAACAGAAAAAAGAAAGCAAACCCAAAGAACACAGACACTGGCAGT
GGACCAGAGTGCCATTAAAAACCACCACGCAGGCGAAGGACAAGATGTAA
AGTAGACTCCTTCTGGATATTGTAGTCAGCCAAAGTACGCCCATCCTCCA
GCTGCTTCCCGGCAAAAATCAACCTCTGCTGATCTGGAGGAATCCCTTCC
TTGTCCTGGATTTTAGCCTTCACATTATCAATGGTGTCTGAACTCTCAAC
CTCCAGGGTAATGGTTTTTCCCGTCAATGTCTTCACGAAAATCTGCATTC
CTCCACGCAGTCTCAACACCAAATGCAGTGTGGATTCCTTCTGAATGTTG
TAATCTGCAAGGGTCCTCCCATCTTCAAGCTGCTTGCCTGCAAAAATCAA
CCTCTGCTGGTCTGGTGGAATGCCCTCCTTATCTTGGATCTTGGCCTTCA
CATTATCAATAGTGTCCGAACTCTCTACCTCCAGAGTGATTGTTTTTCCA
GTGAGAGTCTTCACAAAAATTTGCATGCCACCACGAAGGCGGAGAACCAA
GTGAAGGGTGGACTCCTTCTGAATGTTATAATCTGCAAGGGTCCTACCAT
CTTCAAGCTGCTTGCCAGCAAAAATCAACCTCTGCTGGTCGGGAGGAATG
CCCTCCTTA
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