CN188276

Overview
NameCN188276
Unique NameCN188276
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length587
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 7.026e-20
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 95.9005 bits (237), Expect = 1.565e-19
Identity = 45/74 (60.81%), Postives = 57/74 (77.03%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 9.177e-20
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = -1 
Query:  363 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 587
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 95.9005 bits (237), Expect = 1.565e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -1 
Query:  138 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 359
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.670e-19
Identity = 42/78 (53.85%), Postives = 59/78 (75.64%), Query Frame = -1 
Query:  132 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGEF 365
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG F
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGGNF 78

HSP 2 Score: 92.0485 bits (227), Expect = 2.260e-18
Identity = 41/72 (56.94%), Postives = 56/72 (77.78%), Query Frame = -1 
Query:  366 VKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 581
            V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    5 VRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.015e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 2.260e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.015e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 2.260e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.015e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 2.260e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.015e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 2.260e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.015e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 92.0485 bits (227), Expect = 2.260e-18
Identity = 42/74 (56.76%), Postives = 57/74 (77.03%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 8.589e-18
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 87.4261 bits (215), Expect = 5.567e-17
Identity = 41/74 (55.41%), Postives = 55/74 (74.32%), Query Frame = -1 
Query:  366 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 587
            I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    3 IKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of CN188276 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 1.913e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = -1 
Query:  138 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 365
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76

HSP 2 Score: 88.5817 bits (218), Expect = 2.499e-17
Identity = 40/75 (53.33%), Postives = 57/75 (76.00%), Query Frame = -1 
Query:  363 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 587
            I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77
The following BLAST results are available for this feature:
BLAST of CN188276 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
NEDD8_CAEEL7.026e-2060.53NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1[more]
RUB2_ARATH9.177e-2061.33NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
RUB3_ORYSJ2.670e-1953.85NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
NEDD8_RAT1.015e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.015e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.015e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.015e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.015e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ARATH8.589e-1855.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI1.913e-1753.95NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005M09_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005M09, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188276 ID=CN188276; Name=CN188276; organism=Citrus sinensis; type=EST; length=587bp
GAGCGTTATTATTACCACATAAAACTTTGTGACAAAAAGACTGACACATA
AGCACGACTAACAAACGAAAAATAAATATTACTGCTACAGCATATCATAA
CAGACACGGCGACAAGCACATAACATTCTTAGAATTCACCACCACGAAGG
CGGAGCACAAGATGGAGGGTGGACTCCTTCTGAATGTTATAATCAGCTAA
AGTACGGCCATCCTCAAGCTGCTTCCCAGCAAAGATGAGCCTCTGCTGAT
CTGGTGGAATCCCTTCCTTGTCCTGAATCTTTGCCTTAACATTATCAATA
GTATCTGAGCTCTCCACCTCCAGAGTAATAGTCTTCCCAGTCAAGGTCTT
GACAAATATCTGCATACCACCCCTCAGGCGCAGAACCAGATGAAGGGTCG
ACTCCTTCTGGATGTTATAATCCGCAAGAGTCCTTCCATCCTCAAGCTGT
TTGCCAGCGAAGATCAAACGCTGCTGATCCGGTGGAATTCCTTCCTTATC
TTGGATTTTTGCCTTGACATTGTCAATCGTGTCTGAGCTCTCAACTTCCA
AAGTAATTGTTTTCCCAGTAAGGGTCTTAACAAATAT
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