CN188272

Overview
NameCN188272
Unique NameCN188272
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length755
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN188272_ssr38 microsatellite CN188272_ssr38:38..49. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig5924 contig Csv1_Contig5924:9..763. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_ASPFU (Ubiquitin-conjugating enzyme E2 2 OS=Aspergillus fumigatus GN=ubc2 PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 1.428e-22
Identity = 56/146 (38.36%), Postives = 76/146 (52.05%), Query Frame = 2
Query:  116 NPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            +P AG SA  V + N+  W+  IIGP DT +E G F  +M F   YPN PP VKF ++++HPNVY  G +C+ IL               RW P + V             PN  SPANVEA+  ++D R ++ K+V   V +S E
Sbjct:   19 DPPAGVSASPVAD-NVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPGVKFISQMFHPNVYGTGELCLDIL-------------QNRWSPTYDVAAILTSIQSLLNDPNTSSPANVEASNLYKDNRKEYIKRVRETVEKSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_NEUCR (Ubiquitin-conjugating enzyme E2 2 OS=Neurospora crassa GN=mus-8 PE=3 SV=3)

HSP 1 Score: 106.301 bits (264), Expect = 1.865e-22
Identity = 56/146 (38.36%), Postives = 76/146 (52.05%), Query Frame = 2
Query:  116 NPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            +P AG SA  V + N+  W+  IIGP DT +E G F  +M F   YPN PP+VKF +E++HPNVY  G +C+ IL               RW P + V             PN  S ANVEA+  ++D R ++ K+V   V +S E
Sbjct:   19 DPPAGVSASPVPD-NVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPSVKFISEMFHPNVYATGELCLDIL-------------QNRWSPTYDVAAVLTSIQSLLNDPNTGSRANVEASNLYKDNRKEYHKRVRETVEKSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_CANAL (Ubiquitin-conjugating enzyme E2 2 OS=Candida albicans GN=UBC2 PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.436e-22
Identity = 55/145 (37.93%), Postives = 76/145 (52.41%), Query Frame = 2
Query:  113 KNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            ++P +G SA  + + N+ +W+  IIGP DT +E G F  ++SF   YPN PP VKF +E++HPNVY  G +C+ IL               RW P + V             PN  SPANVEAA  ++D R  + K+V   V  S
Sbjct:   18 QDPPSGVSASPLPD-NVMKWNAVIIGPSDTPFEDGTFRLLLSFDEQYPNKPPQVKFISEMFHPNVYASGELCLDIL-------------QNRWSPTYDVSSILTSVQSLLNDPNISSPANVEAANLYKDHRSLYVKRVRETVENS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBCD6_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=UbcD6 PE=2 SV=2)

HSP 1 Score: 104.76 bits (260), Expect = 5.427e-22
Identity = 53/147 (36.05%), Postives = 76/147 (51.70%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P  G S G   + NI  W+  I GP DT +E G F   + F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN  AA+ +++ R +++K+V  CV +S
Sbjct:   16 LQEDPPTGVS-GAPTDNNIMIWNAVIFGPHDTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKVFHPNVYADGGICLDIL-------------QNRWSPTYDVSAILTSIQSLLSDPNPNSPANSTAAQLYKENRREYEKRVKACVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_SCHPO (Ubiquitin-conjugating enzyme E2 2 OS=Schizosaccharomyces pombe GN=rhp6 PE=1 SV=3)

HSP 1 Score: 104.375 bits (259), Expect = 7.088e-22
Identity = 54/147 (36.73%), Postives = 78/147 (53.06%), Query Frame = 2
Query:  113 KNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            ++P AG SA  V + N+  W+  IIGP DT +E G F  ++SF   YPN PP VKF + ++HPNVY +G +C+ IL               RW P + V             PN+ SPAN EAA+  R+ + ++ ++V + V  S E
Sbjct:   18 QDPPAGVSASPVSD-NVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDIL-------------QNRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRKTVEDSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_DEBHA (Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces hansenii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 9.258e-22
Identity = 54/141 (38.30%), Postives = 72/141 (51.06%), Query Frame = 2
Query:  125 AGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            +G SA  + + N+  W+  IIGP DT +E G F  I+ F   YPN PP+VKF +E++HPNVY  G +C+ IL               RW P + V             PN  SPANVEAA  ++D R  + K+V   V  S
Sbjct:   22 SGVSASPLPD-NVMSWNAVIIGPADTPFEDGTFRLILQFDEQYPNKPPSVKFISEMFHPNVYASGELCLDIL-------------QNRWSPTYDVSSILTSIQSLLNDPNISSPANVEAANLYKDHRSQYIKRVRETVENS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_YEAST (Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces cerevisiae GN=RAD6 PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.209e-21
Identity = 51/132 (38.64%), Postives = 68/132 (51.52%), Query Frame = 2
Query:  158 NIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            N+  W+  IIGP DT YE G F  ++ F   YPN PP VKF +E++HPNVY +G +C+ IL               RW P + V             PN  SPANVEAA  ++D +  + K+V   V +S E
Sbjct:   32 NVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDIL-------------QNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_CANGA (Ubiquitin-conjugating enzyme E2 2 OS=Candida glabrata GN=UBC2 PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.209e-21
Identity = 51/132 (38.64%), Postives = 69/132 (52.27%), Query Frame = 2
Query:  158 NIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            N+  W+  IIGP DT YE G F  ++ F  +YPN PP VKF +E++HPNVY +G +C+ IL               RW P + V             PN  SPANVEAA  ++D +  + K+V   V +S E
Sbjct:   32 NVMVWNAMIIGPADTPYEDGTFRLLLEFDEDYPNKPPHVKFLSEMFHPNVYANGEICLDIL-------------QNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYIKRVKETVEKSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_ASHGO (Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossypii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.209e-21
Identity = 51/132 (38.64%), Postives = 68/132 (51.52%), Query Frame = 2
Query:  158 NIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            N+  W+  IIGP DT YE G F  ++ F   YPN PP VKF +E++HPNVY +G +C+ IL               RW P + V             PN  SPANVEAA  ++D +  + K+V   V +S E
Sbjct:   32 NVMVWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDIL-------------QNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2B_RAT (Ubiquitin-conjugating enzyme E2 B OS=Rattus norvegicus GN=Ube2b PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.579e-21
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P  G S G   E NI +W+  I GP  T +E G F  ++ F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPVGVS-GAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
The following BLAST results are available for this feature:
BLAST of CN188272 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 168
Match NameE-valueIdentityDescription
UBC2_ASPFU1.428e-2238.36Ubiquitin-conjugating enzyme E2 2 OS=Aspergillus f... [more]
UBC2_NEUCR1.865e-2238.36Ubiquitin-conjugating enzyme E2 2 OS=Neurospora cr... [more]
UBC2_CANAL2.436e-2237.93Ubiquitin-conjugating enzyme E2 2 OS=Candida albic... [more]
UBCD6_DROME5.427e-2236.05Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi... [more]
UBC2_SCHPO7.088e-2236.73Ubiquitin-conjugating enzyme E2 2 OS=Schizosacchar... [more]
UBC2_DEBHA9.258e-2238.30Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces ... [more]
UBC2_YEAST1.209e-2138.64Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces... [more]
UBC2_CANGA1.209e-2138.64Ubiquitin-conjugating enzyme E2 2 OS=Candida glabr... [more]
UBC2_ASHGO1.209e-2138.64Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossyp... [more]
UBE2B_RAT1.579e-2134.69Ubiquitin-conjugating enzyme E2 B OS=Rattus norveg... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005M07_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005M07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188272 ID=CN188272; Name=CN188272; organism=Citrus sinensis; type=EST; length=755bp
GAGCCTCAAATATCTGTAGAGGTAGAGCCAATTCCAGCTCTCTCTCTCTA
CTAAAATCATGGCGGCATCTCAGGCAAGTCTCCTCTTGCAAAAACAACTA
AAAGATCTTTGTAAGAACCCTGTTGCTGGGTTTTCTGCCGGGTTGGTGGA
TGAGACAAATATCTTTGAATGGAGTGTTACAATTATTGGACCACCTGATA
CTCTTTATGAGGGGGGATTTTTCAATGCCATTATGAGCTTTCCATCAAAC
TACCCTAATAGCCCACCTACAGTGAAGTTTACAACAGAGATTTGGCATCC
TAATGTCTATCCGGATGGGCGTGTCTGCATATCAATCCTTCATCCTCCAG
GTGACGACCCTAATGGATATGAGCTTGCAAGTGAGCGCTGGATGCCTGTT
CATACTGTTGAAAGTATCGTTTTGAGTATCATATCAATGCTTTCCAGTCC
TAATGATGAATCTCCTGCAAATGTCGAAGCTGCAAAAGAATGGAGAGACA
GGAGAGATGACTTCAAGAAGAAAGTAGGCCGCTGTGTGAGAAGATCACAA
GAAATGTTGTAAACCAACTGCAGGCACTCTTTTTTTTCTCTACCTATGTG
TTTGTACGGGAAATGTCTTGATTTAATTGTGTGGGGGGAACTGGGTTCTG
TGGGTACTTGGTCTTGTGTAGACTGGCATTTTGAAGTTCTCTCAACCATC
TCTCTCGTTTTCTCTGCATGGTCAAGTCACTGTAACGACTGGGAACATCC
ATGTA
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