CN188272

Overview
NameCN188272
Unique NameCN188272
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length755
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN188272_ssr38 microsatellite CN188272_ssr38:38..49. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig5924 contig Csv1_Contig5924:9..763. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2B_RABIT (Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus cuniculus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.579e-21
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P  G S G   E NI +W+  I GP  T +E G F  ++ F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPVGVS-GAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.579e-21
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P  G S G   E NI +W+  I GP  T +E G F  ++ F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPVGVS-GAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.579e-21
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P  G S G   E NI +W+  I GP  T +E G F  ++ F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPVGVS-GAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.579e-21
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P  G S G   E NI +W+  I GP  T +E G F  ++ F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPVGVS-GAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_WHEAT (Ubiquitin-conjugating enzyme E2 2 OS=Triticum aestivum GN=UBC2 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 1.579e-21
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P AG S G   + NI  W+  I GP DT ++GG F   + F  +YPN PPTV+F + ++HPN+Y DG +C+ IL               +W P++ V             PN  SPAN EAA+ + + + ++ +KV   V +S
Sbjct:   16 LQQDPPAGIS-GAPHDNNITLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTVRFVSRMFHPNIYADGSICLDIL-------------QNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMYSENKREYNRKVREVVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_KLULA (Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces lactis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 2.062e-21
Identity = 51/132 (38.64%), Postives = 68/132 (51.52%), Query Frame = 2
Query:  158 NIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRSQE 553
            N+  W+  IIGP DT YE G F  ++ F   YPN PP VKF +E++HPNVY +G +C+ IL               RW P + V             PN  SPANVEAA  ++D +  + K+V   V +S E
Sbjct:   32 NVMIWNAMIIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDIL-------------QNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFQDHKSQYVKRVKETVEKSWE 150          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC3_ARATH (Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis thaliana GN=UBC3 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.694e-21
Identity = 50/147 (34.01%), Postives = 76/147 (51.70%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L K+P  G S G   + NI  W+  I GP DT ++GG F   + F  +YPN PP V+F + ++HPN+Y DG +C+ IL               +W P++ V             PN +SPAN EAA+ + + + ++ +KV   V +S
Sbjct:   16 LQKDPPVGIS-GAPQDNNIMHWNALIFGPEDTPWDGGTFKLTLHFTEDYPNKPPIVRFVSRMFHPNIYADGSICLDIL-------------QNQWSPIYDVAAVLTSIQSLLCDPNPDSPANAEAARLFSENKREYNRKVIEIVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBC2_YARLI (Ubiquitin-conjugating enzyme E2 2 OS=Yarrowia lipolytica GN=UBC2 PE=3 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.694e-21
Identity = 52/145 (35.86%), Postives = 74/145 (51.03%), Query Frame = 2
Query:  113 KNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            ++P  G SA  V + N+  W+  IIGP +T +E G F  ++ F   YPN PP VKF ++++HPNVY  G +C+ IL               RW P + V             PN  SPANVEA+  ++D R  ++K+V   V  S
Sbjct:   18 QDPPQGVSASPVAD-NVLTWNAVIIGPAETPFEDGTFRMVLQFDEQYPNKPPAVKFVSQMFHPNVYSSGELCLDIL-------------QNRWSPTYDVAAILTSVQSLLNDPNTSSPANVEASMLYKDHRQQYEKRVRDTVEAS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2A_MOUSE (Ubiquitin-conjugating enzyme E2 A OS=Mus musculus GN=Ube2a PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.518e-21
Identity = 52/147 (35.37%), Postives = 76/147 (51.70%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P AG S G   E NI  W+  I GP  T +E G F   + F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPAGVS-GAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
BLAST of CN188272 vs. ExPASy Swiss-Prot
Match: UBE2A_HUMAN (Ubiquitin-conjugating enzyme E2 A OS=Homo sapiens GN=UBE2A PE=2 SV=2)

HSP 1 Score: 102.064 bits (253), Expect = 3.518e-21
Identity = 52/147 (35.37%), Postives = 76/147 (51.70%), Query Frame = 2
Query:  107 LCKNPVAGFSAGLVDETNIFEWSVTIIGPPDTLYEGGFFNAIMSFPSNYPNSPPTVKFTTEIWHPNVYPDGRVCISILHPPGDDPNGYELASERWMPVHTVEXXXXXXXXXXXXPNDESPANVEAAKEWRDRRDDFKKKVGRCVRRS 547
            L ++P AG S G   E NI  W+  I GP  T +E G F   + F   YPN PPTV+F ++++HPNVY DG +C+ IL               RW P + V             PN  SPAN +AA+ +++ + +++K+V   V +S
Sbjct:   16 LQEDPPAGVS-GAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDIL-------------QNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQS 148          
The following BLAST results are available for this feature:
BLAST of CN188272 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 168
Match NameE-valueIdentityDescription
UBE2B_RABIT1.579e-2134.69Ubiquitin-conjugating enzyme E2 B OS=Oryctolagus c... [more]
UBE2B_MOUSE1.579e-2134.69Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ... [more]
UBE2B_HUMAN1.579e-2134.69Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ... [more]
UBE2B_BOVIN1.579e-2134.69Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN... [more]
UBC2_WHEAT1.579e-2134.69Ubiquitin-conjugating enzyme E2 2 OS=Triticum aest... [more]
UBC2_KLULA2.062e-2138.64Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces... [more]
UBC3_ARATH2.694e-2134.01Ubiquitin-conjugating enzyme E2 3 OS=Arabidopsis t... [more]
UBC2_YARLI2.694e-2135.86Ubiquitin-conjugating enzyme E2 2 OS=Yarrowia lipo... [more]
UBE2A_MOUSE3.518e-2135.37Ubiquitin-conjugating enzyme E2 A OS=Mus musculus ... [more]
UBE2A_HUMAN3.518e-2135.37Ubiquitin-conjugating enzyme E2 A OS=Homo sapiens ... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005M07_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005M07, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188272 ID=CN188272; Name=CN188272; organism=Citrus sinensis; type=EST; length=755bp
GAGCCTCAAATATCTGTAGAGGTAGAGCCAATTCCAGCTCTCTCTCTCTA
CTAAAATCATGGCGGCATCTCAGGCAAGTCTCCTCTTGCAAAAACAACTA
AAAGATCTTTGTAAGAACCCTGTTGCTGGGTTTTCTGCCGGGTTGGTGGA
TGAGACAAATATCTTTGAATGGAGTGTTACAATTATTGGACCACCTGATA
CTCTTTATGAGGGGGGATTTTTCAATGCCATTATGAGCTTTCCATCAAAC
TACCCTAATAGCCCACCTACAGTGAAGTTTACAACAGAGATTTGGCATCC
TAATGTCTATCCGGATGGGCGTGTCTGCATATCAATCCTTCATCCTCCAG
GTGACGACCCTAATGGATATGAGCTTGCAAGTGAGCGCTGGATGCCTGTT
CATACTGTTGAAAGTATCGTTTTGAGTATCATATCAATGCTTTCCAGTCC
TAATGATGAATCTCCTGCAAATGTCGAAGCTGCAAAAGAATGGAGAGACA
GGAGAGATGACTTCAAGAAGAAAGTAGGCCGCTGTGTGAGAAGATCACAA
GAAATGTTGTAAACCAACTGCAGGCACTCTTTTTTTTCTCTACCTATGTG
TTTGTACGGGAAATGTCTTGATTTAATTGTGTGGGGGGAACTGGGTTCTG
TGGGTACTTGGTCTTGTGTAGACTGGCATTTTGAAGTTCTCTCAACCATC
TCTCTCGTTTTCTCTGCATGGTCAAGTCACTGTAACGACTGGGAACATCC
ATGTA
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