CN188231

Overview
NameCN188231
Unique NameCN188231
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length736
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig5994 contig Csv1_Contig5994:40..775. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UBC2_SCHPO (Ubiquitin-conjugating enzyme E2 2 OS=Schizosaccharomyces pombe GN=rhp6 PE=1 SV=3)

HSP 1 Score: 113.62 bits (283), Expect = 1.117e-24
Identity = 63/143 (44.06%), Postives = 84/143 (58.74%), Query Frame = -2 
Query:  160 TTSVTQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAAL-LNNKEDYRKMVQK 585
            +T+  +RL ++    M      GVSA P  +++  W   I G   T +E  ++KL L F   YP KPP VKF +  FHPNV   G +CLDILQ++WS  YD   IL SIQSLL +PN  SP N+ AA L   NK++Y + V+K
Sbjct:    2 STTARRRLMRDFKR-MQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASPANAEAAQLHRENKKEYVRRVRK 143
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UBC1_COLGL (Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletotrichum gloeosporioides GN=UBC1 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.117e-24
Identity = 58/119 (48.74%), Postives = 76/119 (63.87%), Query Frame = -2 
Query:  220 QRLQKELMSLMMSGGD--LGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPL 570
            +R+ KEL  L   G D     SA P GE +F W  TI G   + Y G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+D+WS A     +LLSI S+L +PNP+ PL
Sbjct:    4 KRINKELTDL---GRDPPSSCSAGPVGEDLFHWQATIMGPGDSPYSGGVFFLAIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSMLTDPNPDEPL 119
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UBC4_SCHPO (Ubiquitin-conjugating enzyme E2 4 OS=Schizosaccharomyces pombe GN=ubc4 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 58/119 (48.74%), Postives = 76/119 (63.87%), Query Frame = -2 
Query:  220 QRLQKELMSLMMSGGD--LGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPL 570
            +R+ +EL  L   G D     SA P G+ +F W  TI G   + Y G  + LS+HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+D+WS A     +LLSI SLL +PNP+ PL
Sbjct:    4 KRINRELADL---GKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPL 119
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UBC2_CANGA (Ubiquitin-conjugating enzyme E2 2 OS=Candida glabrata GN=UBC2 PE=3 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 63/143 (44.06%), Postives = 83/143 (58.04%), Query Frame = -2 
Query:  160 TTSVTQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALL-NNKEDYRKMVQK 585
            +T   +RL ++    M      GVSA P  +++  W   I G   T YE  +++L L F  DYP KPP VKF +  FHPNV   G ICLDILQ++W+  YD  +IL SIQSL  +PNP SP N  AA L  ++K  Y K V++
Sbjct:    2 STPARRRLMRDFKR-MKEDSPPGVSASPLPDNVMVWNAMIIGPADTPYEDGTFRLLLEFDEDYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDVASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYIKRVKE 143
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UB2D3_RAT (Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norvegicus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 57/139 (41.01%), Postives = 81/139 (58.27%), Query Frame = -2 
Query:  154 QRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 570
            +R+ KEL  L         SA P G+ +F W  TI G   + Y+G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+ +WS A     +LLSI SLL +PNP+ PL    A +     D    + +++
Sbjct:    4 KRINKELSDLARDP-PAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREW 141
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UB2D3_PONAB (Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 57/139 (41.01%), Postives = 81/139 (58.27%), Query Frame = -2 
Query:  154 QRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 570
            +R+ KEL  L         SA P G+ +F W  TI G   + Y+G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+ +WS A     +LLSI SLL +PNP+ PL    A +     D    + +++
Sbjct:    4 KRINKELSDLARDP-PAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREW 141
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UB2D3_MOUSE (Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus GN=Ube2d3 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 57/139 (41.01%), Postives = 81/139 (58.27%), Query Frame = -2 
Query:  154 QRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 570
            +R+ KEL  L         SA P G+ +F W  TI G   + Y+G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+ +WS A     +LLSI SLL +PNP+ PL    A +     D    + +++
Sbjct:    4 KRINKELSDLARDP-PAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREW 141
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UB2D3_MACFA (Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fascicularis GN=UBE2D3 PE=2 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 57/139 (41.01%), Postives = 81/139 (58.27%), Query Frame = -2 
Query:  154 QRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 570
            +R+ KEL  L         SA P G+ +F W  TI G   + Y+G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+ +WS A     +LLSI SLL +PNP+ PL    A +     D    + +++
Sbjct:    4 KRINKELSDLARDP-PAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREW 141
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UB2D3_HUMAN (Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens GN=UBE2D3 PE=1 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 1.459e-24
Identity = 57/139 (41.01%), Postives = 81/139 (58.27%), Query Frame = -2 
Query:  154 QRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALLNNKEDYRKMVQKQY 570
            +R+ KEL  L         SA P G+ +F W  TI G   + Y+G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+ +WS A     +LLSI SLL +PNP+ PL    A +     D    + +++
Sbjct:    4 KRINKELSDLARDP-PAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREW 141
[Close]
BLAST of CN188231 vs. ExPASy Swiss-Prot
Match: UBCD1_DROME (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster GN=eff PE=1 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.906e-24
Identity = 60/140 (42.86%), Postives = 86/140 (61.43%), Query Frame = -2 
Query:  160 QRLQKELMSLMMSGGD--LGVSAFPEGESIFTWIGTIEGGKGTMYEGLSYKLSLHFPLDYPFKPPQVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALL-NNKEDYRKMVQK 570
            +R+ KEL  L   G D     SA P G+ +F W  TI G   + Y+G  + L++HFP DYPFKPP+V F T  +HPN++  G+ICLDIL+ +WS A     +LLSI SLL +PNP+ PL    A +   ++E Y ++ ++
Sbjct:    4 KRINKELQDL---GRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNELARE 140
The following BLAST results are available for this feature:
BLAST of CN188231 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 230
Match NameE-valueIdentityDescription
UBC2_SCHPO1.117e-2444.06Ubiquitin-conjugating enzyme E2 2 OS=Schizosacchar... [more]
UBC1_COLGL1.117e-2448.74Ubiquitin-conjugating enzyme E2-16 kDa OS=Colletot... [more]
UBC4_SCHPO1.459e-2448.74Ubiquitin-conjugating enzyme E2 4 OS=Schizosacchar... [more]
UBC2_CANGA1.459e-2444.06Ubiquitin-conjugating enzyme E2 2 OS=Candida glabr... [more]
UB2D3_RAT1.459e-2441.01Ubiquitin-conjugating enzyme E2 D3 OS=Rattus norve... [more]
UB2D3_PONAB1.459e-2441.01Ubiquitin-conjugating enzyme E2 D3 OS=Pongo abelii... [more]
UB2D3_MOUSE1.459e-2441.01Ubiquitin-conjugating enzyme E2 D3 OS=Mus musculus... [more]
UB2D3_MACFA1.459e-2441.01Ubiquitin-conjugating enzyme E2 D3 OS=Macaca fasci... [more]
UB2D3_HUMAN1.459e-2441.01Ubiquitin-conjugating enzyme E2 D3 OS=Homo sapiens... [more]
UBCD1_DROME1.906e-2442.86Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophi... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005L08_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005L08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188231 ID=CN188231; Name=CN188231; organism=Citrus sinensis; type=EST; length=736bp
TTTTTTGAACTTATGTAATCAGTTTTCATTGAAACAGTTCCAAACTGAAA
CAAGCCCTTCCCCTTGAGCTTTACAGAGAAATAATGTGGAAGCCTAACAT
CCGATTTTGTATCTGTTTTGCTGTTCAAATTAGCTCTCAAATTCTCCAGC
AAAGTACTGTTTCTGGACCATTTTTCTGTAATCTTCCTTATTGTTCAACA
GAGCTGCAGCATAGCTGTTGAGAGGGCTCTCTGGATTAGGCTCTCCCAAT
AAACTTTGAATGGACAAAAGAATCGTTCTGCAATCATAAGCTGAAGACCA
CTTGTCCTGAAGGATGTCTAGGCATATGTTGCCATACTGATCAACATTTG
GATGGAAGCACATAGTTTCAAACTTGACTTGGGGTGGCTTGAAAGGATAA
TCCAGAGGAAAATGCAAAGAAAGTTTGTATGATAAACCCTCATACATAGT
TCCCTTTCCACCCTCAATTGTGCCAATCCAAGTGAAAATGCTCTCGCCTT
CAGGAAAGGCCGAGACCCCAAGATCACCTCCACTCATCATAAGAGACATC
AATTCCTTCTGAAGCCTTTGAGTAACGGATGTTGTATCGACGGTGTTAGG
AGAAGAGACAGGCTGCTTCGACGAAGCAGCGGCAGTTGCACGTAAATTTT
CAACCGTCGGATTTGGCCGCACCTCCATGATTAGCAAAGAAGAATCACTG
ATCAACGGCCGATGATCAATGACGATAATCAAGCCT
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