CN188202

Overview
NameCN188202
Unique NameCN188202
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length673
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN188202_ssr201 microsatellite CN188202_ssr201:201..212. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig9569 contig Csv1_Contig9569:987..1659. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_DROME (NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.165e-20
Identity = 45/76 (59.21%), Postives = 59/76 (77.63%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I +++E +D +D +K ++++KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGG 76

HSP 2 Score: 90.8929 bits (224), Expect = 6.599e-18
Identity = 42/74 (56.76%), Postives = 55/74 (74.32%), Query Frame = -1 
Query:  452 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQI 673
            KTLTGK I +++E +D +  +K +++ KEGIPP QQRLIF+GKQ+ D +T ADY +Q  S LHLVL LRGG  I
Sbjct:    6 KTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDSI 79
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 95.9005 bits (237), Expect = 2.051e-19
Identity = 46/74 (62.16%), Postives = 58/74 (78.38%), Query Frame = -1 
Query:  233 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 454
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGG 76

HSP 2 Score: 89.7373 bits (221), Expect = 1.470e-17
Identity = 43/72 (59.72%), Postives = 55/72 (76.39%), Query Frame = -1 
Query:  458 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 673
            KTLTGK I +++E +DTI  +K +++ KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    6 KTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.330e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 87.0409 bits (214), Expect = 9.529e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            KTLTGK I +++E +D +  +K +++ KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    6 KTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.330e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 87.0409 bits (214), Expect = 9.529e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            KTLTGK I +++E +D +  +K +++ KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    6 KTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.330e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 87.0409 bits (214), Expect = 9.529e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            KTLTGK I +++E +D +  +K +++ KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    6 KTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.330e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 87.0409 bits (214), Expect = 9.529e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            KTLTGK I +++E +D +  +K +++ KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    6 KTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.330e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 87.0409 bits (214), Expect = 9.529e-17
Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            KTLTGK I +++E +D +  +K +++ KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    6 KTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.736e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 85.5001 bits (210), Expect = 2.772e-16
Identity = 39/71 (54.93%), Postives = 53/71 (74.65%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            +TLTGK I +++E +DT+  +K +++ +EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    6 RTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.126e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 82.4185 bits (202), Expect = 2.347e-15
Identity = 39/71 (54.93%), Postives = 51/71 (71.83%), Query Frame = -1 
Query:  461 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 673
            KTLT K I +E+E +DTI  +K +I+ KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    6 KTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76
[Close]
BLAST of CN188202 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 88.9669 bits (219), Expect = 2.508e-17
Identity = 41/76 (53.95%), Postives = 57/76 (75.00%), Query Frame = -1 
Query:  233 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 460
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGG 76

HSP 2 Score: 84.3445 bits (207), Expect = 6.176e-16
Identity = 38/72 (52.78%), Postives = 54/72 (75.00%), Query Frame = -1 
Query:  458 KTLTGKTITLEVESSDTIVNVKAKIQVKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 673
            KTLTGK I ++++ +D I  +K +++ KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    6 KTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77
The following BLAST results are available for this feature:
BLAST of CN188202 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99
Match NameE-valueIdentityDescription
NEDD8_DROME3.165e-2059.21NEDD8 OS=Drosophila melanogaster GN=Nedd8 PE=1 SV=... [more]
RUB2_ARATH2.051e-1962.16NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN... [more]
NEDD8_RAT1.330e-1856.58NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1[more]
NEDD8_RABIT1.330e-1856.58NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1[more]
NEDD8_MOUSE1.330e-1856.58NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2[more]
NEDD8_HUMAN1.330e-1856.58NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1[more]
NEDD8_BOVIN1.330e-1856.58NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1[more]
RUB3_ORYSJ1.736e-1853.95NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap... [more]
RUB3_ARATH1.126e-1755.26NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN... [more]
NEDD8_DICDI2.508e-1753.95NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0005K14_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005K14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188202 ID=CN188202; Name=CN188202; organism=Citrus sinensis; type=EST; length=673bp
GAGGTTTAAGTACTGCACATTAAACATGTATTTATTCAAGAAAAAGATTC
TTAGATATTATAATGGGACGTTAGTGGGACCATATCAATATCTTAGAAAC
AGAGTTCAACTACCACAACCAAAATAAACTTCAAATAGTTATTTAAGCCA
TTTCACCAATAGCAAGCAAACATTAACCAAGACAAACCGAAGCAAATAAG
ACACACACACACCCAGATGACCAAACTCAGAAACCTCCACGGAGACGGAG
CACAAGATGCAGAGTGGACTCCTTCTGGATGTTGTAATCAGCAAGAGTCC
TTCCATCCTCAAGTTGTTTTCCAGCAAAGATCAACCTCTGCTGGTCTGGG
GGAATCCCCTCCTTATCCTGGATCTTAGCCTTCACATTGTCAATTGTGTC
CGAACTCTCAACCTCCAATGTGATGGTTTTCCCAGTGAGTGTCTTCACAA
AAATCTGCATACCACCACGGAGGCGAAGAACCAAGTGAAGAGTGGATTCT
TTCTGGATGTTATAGTCTGCAAGAGTCCTTCCATCTTCAAGCTGCTTTCC
AGCAAAAATGAGCCTCTGCTGATCTGGAGGGATACCCTCCTTGACTTGAA
TTTTTGCTTTCACGTTGACGATGGTGTCAGAACTTTCGACCTCAAGAGTA
ATTGTTTTTCCGGTGAGGGTTTT
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