CN187889 | Citrus Genome Database

Overview


Name	CN187889
Unique Name	CN187889
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	911

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Pulp cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: FRMA_ECOLI (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain K12) GN=frmA PE=1 SV=3)

HSP 1 Score: 168.703 bits (426), Expect = 2.961e-44
Identity = 83/192 (43.23%), Postives = 115/192 (59.90%), Query Frame = -3

Query:  271 ATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCIIR 846
            A  N AK + G SVA+F            AR + A RII +D NP +F+ A++FG T+ +NP DYDKP++ V+ D+   G+D + EC G+V  M +A E  H GWG +V++GV        T P  L+  R  KG+ FG  K RS LP +VE  M  ++++E F+THT+   EIN AFDLM  G+SIR +IR
Sbjct:  177 AVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAVAGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIR 368

HSP 2 Score: 31.5722 bits (70), Expect = 2.961e-44
Identity = 12/22 (54.55%), Postives = 16/22 (72.73%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            INP A  + VC+L CG++TG G
Sbjct:  155 INPEANHEHVCLLGCGVTTGIG 176

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1A_MACMU (Alcohol dehydrogenase 1A OS=Macaca mulatta GN=ADH1A PE=2 SV=2)

HSP 1 Score: 164.081 bits (414), Expect = 5.028e-44
Identity = 77/193 (39.90%), Postives = 119/193 (61.66%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + V VAK   G + A+F             + +GA+RII VD+N  +F +AK+ G TE +NP+DY KP+Q+V+ +MT+GGVD S E  G +  M+++  C H+  G +V+VGVP        +PM LL  RT KG  +G +K + D+P +V  +M K+  ++  ITH +PF +IN+ FDL+ +G+SIR I+
Sbjct:  181 YGSAVKVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPDSQNLSINPMLLLTGRTWKGAVYGGFKSKEDIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLRSGKSIRTIL 373

HSP 2 Score: 35.4242 bits (80), Expect = 5.028e-44
Identity = 12/22 (54.55%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+P++KVC++ CG STG+G
Sbjct:  161 IDAASPMEKVCLIGCGFSTGYG 182

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1B_PAPHA (Alcohol dehydrogenase 1B OS=Papio hamadryas GN=ADH1B PE=2 SV=2)

HSP 1 Score: 163.31 bits (412), Expect = 5.028e-44
Identity = 77/193 (39.90%), Postives = 118/193 (61.14%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + VNVAK   G   A+F             + +GA+RII VD+N  +F +AK+ G TE +NP+DY KP+Q+V+ +MT+GGVD S E  G +  M+++  C H+  G +V+VGVP        +PM LL  RT KG  +G +K R  +P +V  +M K+  ++  ITH +PF +IN+ FDL+ +G+SIR ++
Sbjct:  181 YGSAVNVAKVTPGSVCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPDSQNLSINPMLLLTGRTWKGAVYGGFKSREGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLRSGKSIRTVL 373

HSP 2 Score: 36.1946 bits (82), Expect = 5.028e-44
Identity = 13/22 (59.09%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+PL+KVC++ CG STG+G
Sbjct:  161 IDAASPLEKVCLIGCGFSTGYG 182

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1B_PANTR (Alcohol dehydrogenase 1B OS=Pan troglodytes GN=ADH1B PE=2 SV=3)

HSP 1 Score: 162.925 bits (411), Expect = 6.552e-44
Identity = 76/193 (39.38%), Postives = 119/193 (61.66%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + VNVAK   G + A+F             + +GA+RII VD+N  +F +AK+ G TE +NP+DY KP+Q+V+ +MT+GGVD S E  G +  M+++  C H+  G +V+VGVP        +PM LL  RT KG  +G +K +  +P +V  +M K+  ++  ITH +PF +IN+ FDL+ +G+SIR ++
Sbjct:  181 YGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVL 373

HSP 2 Score: 36.1946 bits (82), Expect = 6.552e-44
Identity = 13/22 (59.09%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+PL+KVC++ CG STG+G
Sbjct:  161 IDAASPLEKVCLIGCGFSTGYG 182

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1B_HUMAN (Alcohol dehydrogenase 1B OS=Homo sapiens GN=ADH1B PE=1 SV=2)

HSP 1 Score: 162.925 bits (411), Expect = 6.552e-44
Identity = 76/193 (39.38%), Postives = 119/193 (61.66%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + VNVAK   G + A+F             + +GA+RII VD+N  +F +AK+ G TE +NP+DY KP+Q+V+ +MT+GGVD S E  G +  M+++  C H+  G +V+VGVP        +PM LL  RT KG  +G +K +  +P +V  +M K+  ++  ITH +PF +IN+ FDL+ +G+SIR ++
Sbjct:  181 YGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVL 373

HSP 2 Score: 36.1946 bits (82), Expect = 6.552e-44
Identity = 13/22 (59.09%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+PL+KVC++ CG STG+G
Sbjct:  161 IDAASPLEKVCLIGCGFSTGYG 182

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: FRMA_ECOSM (S-(hydroxymethyl)glutathione dehydrogenase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=frmA PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 6.553e-44
Identity = 82/192 (42.71%), Postives = 114/192 (59.38%), Query Frame = -3

Query:  271 ATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCIIR 846
            A  N AK +   SVA+F            AR + A RII +D NP +F+ A++FG T+ +NP DYDKP++ V+ D+   G+D + EC G+V  M +A E  H GWG +V++GV        T P  L+  R  KG+ FG  K RS LP +VE  M  ++++E F+THT+   EIN AFDLM  G+SIR +IR
Sbjct:  177 AVHNTAKVQPDDSVAVFGLGAIGLAVVQGARQAKAGRIIAIDTNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALESAHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGDIDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIR 368

HSP 2 Score: 31.5722 bits (70), Expect = 6.553e-44
Identity = 12/22 (54.55%), Postives = 16/22 (72.73%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            INP A  + VC+L CG++TG G
Sbjct:  155 INPEANHEHVCLLGCGVTTGIG 176

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1_PERMA (Alcohol dehydrogenase 1 OS=Peromyscus maniculatus GN=ADH1 PE=2 SV=2)

HSP 1 Score: 162.155 bits (409), Expect = 1.450e-43
Identity = 79/193 (40.93%), Postives = 118/193 (61.14%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + V VAK   G + A+F             + +GA+RII VD+N  +F +AK+ G TE +NP DY KP+Q+V+ +MT+GGVD S E  G +  M S+    H   GV+V+VGVP    +   +PM+LL  R+ KG  FG +K +  +P +V  +M K+  +E  ITH +PF +IN+AFDL+ AG+SIR ++
Sbjct:  181 YGSAVKVAKVTPGSTCAVFGLGGVGLSVIIGCKAAGAARIIAVDINKDKFAKAKELGATECINPLDYSKPIQEVLQEMTDGGVDFSFEVIGRLDTMTSSLLSCHASCGVSVIVGVPPNAQSLSVNPMSLLMGRSWKGAIFGGFKSKDSVPKLVTDFMAKKFPLEPLITHVLPFEKINEAFDLLRAGKSIRTVL 373

HSP 2 Score: 35.8094 bits (81), Expect = 1.450e-43
Identity = 13/22 (59.09%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+PL+KVC++ CG STG+G
Sbjct:  161 IDGASPLEKVCLIGCGFSTGYG 182

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1_CHICK (Alcohol dehydrogenase 1 OS=Gallus gallus GN=ADH1 PE=1 SV=2)

HSP 1 Score: 158.688 bits (400), Expect = 1.450e-43
Identity = 75/193 (38.86%), Postives = 117/193 (60.62%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + A +  AK + G + A+F             + +GASRII VD+N  +F +AK+ G TE +NPKD+ KP+ +V+ +MT  GVD S E  G ++ M +A    H+ +GV+V+VGVP         PM + + RT KG+ FG +K +  +P +V  YM K+  ++  ITHT+PF++IN+ FDL+  G+SIR ++
Sbjct:  182 YGAVLQTAKVEAGSTCAVFGLGGVGLSVVMGCKAAGASRIIAVDINKDKFAKAKELGATECINPKDFKKPIHEVLTEMTGQGVDYSFEVIGRIETMTAALASCHNNYGVSVIVGVPPAAQKISFDPMLIFSGRTWKGSVFGGWKSKDAVPKLVADYMKKKFVLDPLITHTLPFTKINEGFDLLRTGKSIRSVL 374

HSP 2 Score: 39.2762 bits (90), Expect = 1.450e-43
Identity = 15/27 (55.56%), Postives = 22/27 (81.48%), Query Frame = -1

Query:  831 INPAAPLDKVCVLSCGISTGFGLLLMS 911
            I+ AAPL+KVC++ CG STG+G +L +
Sbjct:  162 IDSAAPLEKVCLIGCGFSTGYGAVLQT 188

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1A_HUMAN (Alcohol dehydrogenase 1A OS=Homo sapiens GN=ADH1A PE=1 SV=2)

HSP 1 Score: 161.384 bits (407), Expect = 1.889e-43
Identity = 77/193 (39.90%), Postives = 118/193 (61.14%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + VNVAK   G + A+F             + +GA+RII VD+N  +F +AK+ G TE +NP+DY KP+Q+V+ +MT+GGVD S E  G +  M+++  C H+  G +V+VGVP        +PM LL  RT KG   G +K +  +P +V  +M K+  ++  ITH +PF +IN+ FDL+ +G+SIR I+
Sbjct:  181 YGSAVNVAKVTPGSTCAVFGLGGVGLSAIMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPDSQNLSMNPMLLLTGRTWKGAILGGFKSKECVPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTIL 373

HSP 2 Score: 36.1946 bits (82), Expect = 1.889e-43
Identity = 13/22 (59.09%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+PL+KVC++ CG STG+G
Sbjct:  161 IDAASPLEKVCLIGCGFSTGYG 182

[Close]

BLAST of CN187889 vs. ExPASy Swiss-Prot
Match: ADH1_MOUSE (Alcohol dehydrogenase 1 OS=Mus musculus GN=Adh1 PE=2 SV=2)

HSP 1 Score: 159.844 bits (403), Expect = 2.462e-43
Identity = 79/193 (40.93%), Postives = 117/193 (60.62%), Query Frame = -3

Query:  274 FWATVNVAKPKKGQSVAIFXXXXXXXXXXXXARVSGASRIIGVDLNPSRFNEAKKFGVTEFVNPKDYDKPVQQVIADMTNGGVDRSVECTGSVQAMISAFECVHDGWGVAVLVGVPNKDDAFKTHPMNLLNERTLKGTFFGNYKPRSDLPSVVEKYMNKELEVEKFITHTVPFSEINKAFDLMLAGESIRCII 852
            + + V VAK   G + A+F             + +GA+RII VD+N  +F +AK+ G TE +NP+DY KP+Q+V+ +MT+GGVD S E  G +  M SA    H   GV+V+VGVP        +PM LL  RT KG  FG +K +  +P +V  +M K+  ++  ITH +PF +IN+AFDL+ +G+SIR ++
Sbjct:  181 YGSAVKVAKVTPGSTCAVFGLGGVGLSVIIGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYSKPIQEVLQEMTDGGVDFSFEVIGRLDTMTSALLSCHAACGVSVVVGVPPNAQNLSMNPMLLLLGRTWKGAIFGGFKSKDSVPKLVADFMAKKFPLDPLITHVLPFEKINEAFDLLRSGKSIRTVL 373

HSP 2 Score: 37.3502 bits (85), Expect = 2.462e-43
Identity = 14/22 (63.64%), Postives = 19/22 (86.36%), Query Frame = -1

Query:  846 INPAAPLDKVCVLSCGISTGFG 911
            I+ A+PLDKVC++ CG STG+G
Sbjct:  161 IDGASPLDKVCLIGCGFSTGYG 182

The following BLAST results are available for this feature:

BLAST of CN187889 vs. ExPASy Swiss-Prot

BLAST of CN187889 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 112


Match Name	E-value	Identity	Description
FRMA_ECOLI	2.961e-44	43.23	S-(hydroxymethyl)glutathione dehydrogenase OS=Esch...	[more]
ADH1A_MACMU	5.028e-44	39.90	Alcohol dehydrogenase 1A OS=Macaca mulatta GN=ADH1...	[more]
ADH1B_PAPHA	5.028e-44	39.90	Alcohol dehydrogenase 1B OS=Papio hamadryas GN=ADH...	[more]
ADH1B_PANTR	6.552e-44	39.38	Alcohol dehydrogenase 1B OS=Pan troglodytes GN=ADH...	[more]
ADH1B_HUMAN	6.552e-44	39.38	Alcohol dehydrogenase 1B OS=Homo sapiens GN=ADH1B ...	[more]
FRMA_ECOSM	6.553e-44	42.71	S-(hydroxymethyl)glutathione dehydrogenase OS=Esch...	[more]
ADH1_PERMA	1.450e-43	40.93	Alcohol dehydrogenase 1 OS=Peromyscus maniculatus ...	[more]
ADH1_CHICK	1.450e-43	38.86	Alcohol dehydrogenase 1 OS=Gallus gallus GN=ADH1 P...	[more]
ADH1A_HUMAN	1.889e-43	39.90	Alcohol dehydrogenase 1A OS=Homo sapiens GN=ADH1A ...	[more]
ADH1_MOUSE	2.462e-43	40.93	Alcohol dehydrogenase 1 OS=Mus musculus GN=Adh1 PE...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS05_0005C19_f Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005C19, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN187889 ID=CN187889; Name=CN187889; organism=Citrus sinensis; type=EST; length=911bp
GGGGGGGGGGGGGGGCGAGACCCCCTTAGAAAACACTCTCTTTGTACGCC
ATATGCAGCAAGTCATCAAACAGAAAAACAAAACAAAAAAAGGGACTGTA
AGAAATATGTAGAAAACACAACTCTTTGAAAGCCATTCGCAGCAAGTCAT
CAAACATACAGCATAAATTACTTATTCTCGGGGTAATATATAACTTGGAA
AATTATGTGATCATCTTCATTCTGCCTCCATTAGATTGATCAGCAACAGC
AGACACATCTAACCATCCATGCGAATGATGCACCTAATGCTTTCTCCCGC
AAGCATGAGGTCAAATGCCTTGTTTATCTCCGAGAAGGGCACAGTGTGAG
TGATGAATTTCTCGACCTCCAACTCCTTGTTCATGTATTTTTCAACAACA
GAAGGAAGATCAGAGCGAGGTTTGTAGTTGCCAAAGAAGGTACCCTTAAG
AGTCCTCTCATTAAGCAGATTCATGGGATGAGTCTTGAATGCATCATCTT
TGTTTGGCACACCCACAAGCACAGCAACACCCCAACCATCGTGAACGCAT
TCAAATGCTGAAATCATGGCCTGGACGCTTCCAGTACATTCAACACTTCT
ATCCACTCCACCATTGGTCATGTCAGCAATCACCTGTTGAACAGGTTTGT
CATAATCTTTTGGATTCACAAACTCGGTAACCCCAAACTTCTTGGCTTCA
TTGAATCTGCTTGGGTTCAAATCAACACCAATGATCCTGGAAGCCCCTGA
TACCCTTGCCCCTTCGGCAGCAGCGAGGCCAACTGCACCCAATCCAAAAA
TGGCAACAGACTGGCCCTTCTTCGGTTTGGCGACATTAACAGTAGCCCAA
AACCTGTGGAGATTCCACAGCTAAGCACACAAACTTTGTCAAGAGGGGCA
GCAGGATTGAT