CK938755 | Citrus Genome Database

Overview


Name	CK938755
Unique Name	CK938755
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	876

Libraries


Library Name	Type
Developing fruit albedo at 165 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: SGK1_BOVIN (Serine/threonine-protein kinase Sgk1 OS=Bos taurus GN=SGK1 PE=2 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.164e-32
Identity = 69/138 (50.00%), Postives = 96/138 (69.57%), Query Frame = 2

Query:  428 DFEVLKVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTK-IDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            DF  LKV+G+G+FG+V   R K     YA+KV++K  I++K   +++ +ER++L K + HPF+V L +SFQT  +LY VLD+INGG LF+ L     F E  AR YAAEI SA+ +LH+  I++RDLKPENILL + G
Sbjct:   97 DFHFLKVIGKGSFGKVLLARHKAEEAFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQG 234

HSP 2 Score: 20.7866 bits (42), Expect = 1.164e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            ++LTDFGL K+
Sbjct:  236 IVLTDFGLCKE 246

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: SGK1_RAT (Serine/threonine-protein kinase Sgk1 OS=Rattus norvegicus GN=Sgk1 PE=1 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 1.164e-32
Identity = 69/138 (50.00%), Postives = 96/138 (69.57%), Query Frame = 2

Query:  428 DFEVLKVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTK-IDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            DF  LKV+G+G+FG+V   R K     YA+KV++K  I++K   +++ +ER++L K + HPF+V L +SFQT  +LY VLD+INGG LF+ L     F E  AR YAAEI SA+ +LH+  I++RDLKPENILL + G
Sbjct:   97 DFHFLKVIGKGSFGKVLLARHKAEEAFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQG 234

HSP 2 Score: 20.7866 bits (42), Expect = 1.164e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            ++LTDFGL K+
Sbjct:  236 IVLTDFGLCKE 246

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: SGK1_MOUSE (Serine/threonine-protein kinase Sgk1 OS=Mus musculus GN=Sgk1 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.516e-32
Identity = 69/138 (50.00%), Postives = 96/138 (69.57%), Query Frame = 2

Query:  428 DFEVLKVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTK-IDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            DF  LKV+G+G+FG+V   R K     YA+KV++K  I++K   +++ +ER++L K + HPF+V L +SFQT  +LY VLD+INGG LF+ L     F E  AR YAAEI SA+ +LH+  I++RDLKPENILL + G
Sbjct:   97 DFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQG 234

HSP 2 Score: 20.7866 bits (42), Expect = 1.516e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            ++LTDFGL K+
Sbjct:  236 IVLTDFGLCKE 246

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: SGK1_MACFA (Serine/threonine-protein kinase Sgk1 OS=Macaca fascicularis GN=SGK1 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.516e-32
Identity = 69/138 (50.00%), Postives = 96/138 (69.57%), Query Frame = 2

Query:  428 DFEVLKVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTK-IDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            DF  LKV+G+G+FG+V   R K     YA+KV++K  I++K   +++ +ER++L K + HPF+V L +SFQT  +LY VLD+INGG LF+ L     F E  AR YAAEI SA+ +LH+  I++RDLKPENILL + G
Sbjct:   97 DFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQG 234

HSP 2 Score: 20.7866 bits (42), Expect = 1.516e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            ++LTDFGL K+
Sbjct:  236 IVLTDFGLCKE 246

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: SGK1_HUMAN (Serine/threonine-protein kinase Sgk1 OS=Homo sapiens GN=SGK1 PE=1 SV=2)

HSP 1 Score: 138.272 bits (347), Expect = 1.516e-32
Identity = 69/138 (50.00%), Postives = 96/138 (69.57%), Query Frame = 2

Query:  428 DFEVLKVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTK-IDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            DF  LKV+G+G+FG+V   R K     YA+KV++K  I++K   +++ +ER++L K + HPF+V L +SFQT  +LY VLD+INGG LF+ L     F E  AR YAAEI SA+ +LH+  I++RDLKPENILL + G
Sbjct:   97 DFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQG 234

HSP 2 Score: 20.7866 bits (42), Expect = 1.516e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            ++LTDFGL K+
Sbjct:  236 IVLTDFGLCKE 246

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: KS6A5_HUMAN (Ribosomal protein S6 kinase alpha-5 OS=Homo sapiens GN=RPS6KA5 PE=1 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 1.959e-32
Identity = 73/146 (50.00%), Postives = 102/146 (69.86%), Query Frame = 2

Query:  416 VGIEDFEVLKVVGQGAFGRVYQVRK---KNTSEIYAMKVMRKDKIVEK-NHAEYMKAERDILTKI-DHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            VGIE+FE+LKV+G GA+G+V+ VRK    +T ++YAMKV++K  IV+K    E+ + ER +L  I   PF+V L Y+FQT+ +L+L+LD+INGG LF  L     F E   +IY  EIV A+ HLH  GI++RD+K ENILL ++G
Sbjct:   44 VGIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNG 189

HSP 2 Score: 23.8682 bits (50), Expect = 1.959e-32
Identity = 9/12 (75.00%), Postives = 12/12 (100.00%), Query Frame = 1

Query:  838 VMLTDFGLAKQF 873
            V+LTDFGL+K+F
Sbjct:  191 VVLTDFGLSKEF 202

HSP 3 Score: 74.7146 bits (182), Expect = 7.618e-13
Identity = 42/127 (33.07%), Postives = 70/127 (55.12%), Query Frame = 2

Query:  443 KVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKIDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENIL 823
            K +G+G+F    +   K +++ +A+K++ K     +  A   K    +     HP +V+L   F  +   +LV++ +NGG LF ++     F E  A     ++VSAVSH+H  G++HRDLKPEN+L
Sbjct:  430 KPLGEGSFSICRKCVHKKSNQAFAVKIISK-----RMEANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFERIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLL 551

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: KS6A6_MOUSE (Ribosomal protein S6 kinase alpha-6 OS=Mus musculus GN=Rps6ka6 PE=2 SV=2)

HSP 1 Score: 138.272 bits (347), Expect = 1.960e-32
Identity = 69/139 (49.64%), Postives = 100/139 (71.94%), Query Frame = 2

Query:  431 FEVLKVVGQGAFGRVYQVRKK---NTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKIDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            F++LKV+GQG+FG+V+ VRKK   +  ++YAMKV+RK  +  ++     K ERDIL +++HPF+V+L Y+FQT+ +LYL+LDF+ GG +F +L    LF E+  + Y AE+  A+ HLH  GI++RDLKPENILL   G
Sbjct:   93 FDLLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLRKASLKVRDRVR-TKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVLFTEEDVKFYLAELALALDHLHRLGIVYRDLKPENILLDEIG 230

HSP 2 Score: 20.4014 bits (41), Expect = 1.960e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            + LTDFGL+K+
Sbjct:  232 IKLTDFGLSKE 242

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: KS6A6_HUMAN (Ribosomal protein S6 kinase alpha-6 OS=Homo sapiens GN=RPS6KA6 PE=1 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 1.961e-32
Identity = 69/139 (49.64%), Postives = 100/139 (71.94%), Query Frame = 2

Query:  431 FEVLKVVGQGAFGRVYQVRKK---NTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKIDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            FE+LKV+GQG+FG+V+ VRKK   +  ++YAMKV++K  +  ++     K ERDIL +++HPF+V+L Y+FQT+ +LYL+LDF+ GG +F +L    LF E+  + Y AE+  A+ HLH  GI++RDLKPENILL   G
Sbjct:   73 FELLKVLGQGSFGKVFLVRKKTGPDAGQLYAMKVLKKASLKVRDRVR-TKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVLFTEEDVKFYLAELALALDHLHQLGIVYRDLKPENILLDEIG 210

HSP 2 Score: 20.4014 bits (41), Expect = 1.961e-32
Identity = 7/11 (63.64%), Postives = 10/11 (90.91%), Query Frame = 1

Query:  838 VMLTDFGLAKQ 870
            + LTDFGL+K+
Sbjct:  212 IKLTDFGLSKE 222

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: KS6A5_PONAB (Ribosomal protein S6 kinase alpha-5 OS=Pongo abelii GN=RPS6KA5 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.551e-32
Identity = 72/146 (49.32%), Postives = 101/146 (69.18%), Query Frame = 2

Query:  416 VGIEDFEVLKVVGQGAFGRVYQVRK---KNTSEIYAMKVMRKDKIVEK-NHAEYMKAERDILTKI-DHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            VGIE+FE+LKV+G GA+G+V+ VRK    +T ++YAMKV++K  IV+K    E+ + ER +L      PF+V L Y+FQT+ +L+L+LD+INGG LF  L     F E   +IY  EIV A+ HLH  GI++RD+K ENILL ++G
Sbjct:   44 VGIENFELLKVLGTGAYGKVFLVRKISGHDTGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHTRQSPFLVTLHYAFQTETKLHLILDYINGGELFTHLSQRERFTEHEVQIYVGEIVLALEHLHKLGIIYRDIKLENILLDSNG 189

HSP 2 Score: 25.409 bits (54), Expect = 2.551e-32
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = 1

Query:  838 VMLTDFGLAKQF 873
            VMLTDFGL+K+F
Sbjct:  191 VMLTDFGLSKEF 202

HSP 3 Score: 74.7146 bits (182), Expect = 7.618e-13
Identity = 42/127 (33.07%), Postives = 70/127 (55.12%), Query Frame = 2

Query:  443 KVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKIDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENIL 823
            K +G+G+F    +   K +++ +A+K++ K     +  A   K    +     HP +V+L   F  +   +LV++ +NGG LF ++     F E  A     ++VSAVSH+H  G++HRDLKPEN+L
Sbjct:  430 KPLGEGSFSICRKCVHKKSNQAFAVKIISK-----RMEANTQKEITALKLCEGHPNIVKLHEVFHDQLHTFLVMELLNGGELFERIKKKKHFSETEASYIMRKLVSAVSHMHDVGVVHRDLKPENLL 551

[Close]

BLAST of CK938755 vs. ExPASy Swiss-Prot
Match: KS6A5_CHICK (Ribosomal protein S6 kinase alpha-5 OS=Gallus gallus GN=RPS6KA5 PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 2.551e-32
Identity = 73/146 (50.00%), Postives = 101/146 (69.18%), Query Frame = 2

Query:  416 VGIEDFEVLKVVGQGAFGRVYQVRK---KNTSEIYAMKVMRKDKIVEK-NHAEYMKAERDILTKI-DHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENILLGADG 838
            VGIE+FE+LKV+G GA+G+V+ VRK    +  ++YAMKV++K  IV+K    E+ + ER +L  I   PF+V L Y+FQT  +L+L+LD+INGG LF  L     F E+  +IY  EIV A+ HLH  GI++RD+K ENILL +DG
Sbjct:   34 VGIENFELLKVLGTGAYGKVFLVRKVSGHDAGKLYAMKVLKKATIVQKAKTTEHTRTERQVLEHIRQSPFLVTLHYAFQTDTKLHLILDYINGGELFTHLSQRERFSENEVQIYIGEIVLALEHLHKLGIIYRDIKLENILLDSDG 179

HSP 2 Score: 23.8682 bits (50), Expect = 2.551e-32
Identity = 9/12 (75.00%), Postives = 12/12 (100.00%), Query Frame = 1

Query:  838 VMLTDFGLAKQF 873
            V+LTDFGL+K+F
Sbjct:  181 VVLTDFGLSKEF 192

HSP 3 Score: 71.2478 bits (173), Expect = 8.423e-12
Identity = 44/127 (34.65%), Postives = 69/127 (54.33%), Query Frame = 2

Query:  443 KVVGQGAFGRVYQVRKKNTSEIYAMKVMRKDKIVEKNHAEYMKAERDILTKIDHPFVVQLKYSFQTKYRLYLVLDFINGGHLFFQLYHHGLFREDLARIYAAEIVSAVSHLHANGIMHRDLKPENIL 823
            K +G+G+F    +   K TS+ YA+K++ K   +E N    + A   +     HP VV+L   +  +   +LV++ + GG L  ++     F E  A      +VSAVSH+H  G++HRDLKPEN+L
Sbjct:  420 KPLGEGSFSICRKCLHKKTSQEYAVKIISKR--MEANTQREITA---LKLCEGHPNVVKLHEVYHDQLHTFLVMELLKGGELLERIQKKQHFSETEASHIMRRLVSAVSHMHDVGVVHRDLKPENLL 541

The following BLAST results are available for this feature:

BLAST of CK938755 vs. ExPASy Swiss-Prot

BLAST of CK938755 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
SGK1_BOVIN	1.164e-32	50.00	Serine/threonine-protein kinase Sgk1 OS=Bos taurus...	[more]
SGK1_RAT	1.164e-32	50.00	Serine/threonine-protein kinase Sgk1 OS=Rattus nor...	[more]
SGK1_MOUSE	1.516e-32	50.00	Serine/threonine-protein kinase Sgk1 OS=Mus muscul...	[more]
SGK1_MACFA	1.516e-32	50.00	Serine/threonine-protein kinase Sgk1 OS=Macaca fas...	[more]
SGK1_HUMAN	1.516e-32	50.00	Serine/threonine-protein kinase Sgk1 OS=Homo sapie...	[more]
KS6A5_HUMAN	1.959e-32	50.00	Ribosomal protein S6 kinase alpha-5 OS=Homo sapien...	[more]
KS6A6_MOUSE	1.960e-32	49.64	Ribosomal protein S6 kinase alpha-6 OS=Mus musculu...	[more]
KS6A6_HUMAN	1.961e-32	49.64	Ribosomal protein S6 kinase alpha-6 OS=Homo sapien...	[more]
KS6A5_PONAB	2.551e-32	49.32	Ribosomal protein S6 kinase alpha-5 OS=Pongo abeli...	[more]
KS6A5_CHICK	2.551e-32	50.00	Ribosomal protein S6 kinase alpha-5 OS=Gallus gall...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004438_E12 Developing fruit albedo at 165 DAFB Citrus sinensis cDNA clone A1650002_IIIF_E12 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK938755 ID=CK938755; Name=CK938755; organism=Citrus sinensis; type=EST; length=876bp
GGGGTACAAAACCTGCATGCATAAACCACTTCAGACNNNNCTGTTTTTTC
CTGTGAGTCCCCCAGATGATGTTGTGTCTGATTCGCTTGAGTTAGATTTC
TCCGATGTATTTGGTCCTGTTCCAGTTCAGGATTCGCCAGAAGTGAGTTA
TGGGGATTTAGTGAATGCTGTATCTGCTGCAGCAGATGCAAGTGAGATAA
TTTATGAAGATCCAGTTATCATATGTAACCGATCACATTCTCTAGTTGGT
CCCTCTTCCTGTGTTAGTGAATCATCGAAGCTGACCAAGCTCATTCTGCG
TGAATCAGAGGATGCGGAGGAACTACTTGAGTGTGTTGATGGATTGACCG
TCAAAGAAATTGATGAATCCTCCATTGATGAAAATGTCAAAGGAGATTCT
GTGACCGTCTGCTGTGTAGGGATTGAGGATTTTGAGGTTTTGAAAGTTGT
TGGGCAGGGTGCATTTGGAAGGGTATATCAAGTGAGGAAAAAGAATACAT
CCGAAATATATGCAATGAAGGTCATGCGGAAGGACAAGATAGTGGAGAAG
AATCATGCAGAATACATGAAAGCTGAGAGGGATATTTTGACAAAAATAGA
TCACCCCTTTGTTGTCCAGCTCAAATACTCATTCCAAACCAAATATCGAT
TATACCTAGTTCTTGACTTCATAAATGGTGGCCACCTTTTTTTCCAGCTC
TATCACCATGGCCTATTTAGAGAGGACCTGGCACGAATATATGCTGCCGA
GATTGTTTCTGCTGTCTCCCACCTTCATGCTAATGGCATAATGCACAGGG
ATCTTAAACCTGAAAATATTCTTTTGGGTGCAGATGGGTAATGCTGACTG
ATTTTGGCCTTGCAAAGCAATTTGAT