CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC7_YEAST (Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharomyces cerevisiae GN=UBC7 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 7.830e-24
Identity = 36/71 (50.70%), Postives = 44/71 (61.97%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAED-MFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGP 404
            A KR+LKEL+ L KD P    AGP +E+ +F W   I GP D+PYA GVF   + FP DYP  PPK+   P
Sbjct:    5 AQKRLLKELQQLIKDSPPGIVAGPKSENNIFIWDCLIQGPPDTPYADGVFNAKLEFPKDYPLSPPKLTFTP 75

HSP 2 Score: 54.299 bits (129), Expect = 7.830e-24
Identity = 25/84 (29.76%), Postives = 46/84 (54.76%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDIL-------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            PK  F   + HPNI  NG +C+ IL             +E+WSP  ++ K+LLS+ S+L++PN +     +   +++ ++ ++E
Sbjct:   69 PKLTFTPSILHPNIYPNGEVCISILHSPGDDPNMYELAEERWSPVQSVEKILLSVMSMLSEPNIESGANIDACILWRDNRPEFE 152
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC2_CRYNE (Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus neoformans GN=UBC2 PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 1.019e-23
Identity = 31/67 (46.27%), Postives = 46/67 (68.66%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            F +K+FHPNI +NG +CLDIL+ +WSP   ++ +L S+ SLL DPNP  P   + A ++K +  +YE
Sbjct:   72 FISKMFHPNIYANGELCLDILQNRWSPTYDVAAILTSVQSLLNDPNPASPANVDAAQLFKENLKEYE 138

HSP 2 Score: 56.225 bits (134), Expect = 1.019e-23
Identity = 24/66 (36.36%), Postives = 37/66 (56.06%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A +R++++ K L  D P   S  P  +++  W A I GP ++P+  G F +T+ F   YP KPP V
Sbjct:    5 AKRRLIRDFKRLTSDAPIGISGSPNPDNIMVWNAVIFGPPETPFEDGSFRLTLTFTDAYPNKPPTV 70
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2SC_XENLA (Ubiquitin-conjugating enzyme E2 S-C OS=Xenopus laevis GN=ube2s-C PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.319e-23
Identity = 38/89 (42.70%), Postives = 54/89 (60.67%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAMG*RLLG 649
            PKG F TK+FHPN+++NG IC+++LK+ W   L I  VLL+I  LL  PNP+  L  E   +   +  +Y S A+  T+ +A G  L G
Sbjct:   75 PKGYFLTKIFHPNVSNNGEICVNVLKKDWKAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYASRAKLMTEIHAQGSTLRG 163

HSP 2 Score: 51.2174 bits (121), Expect = 1.319e-23
Identity = 24/63 (38.10%), Postives = 33/63 (52.38%), Query Frame = 3 
Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +R+ KE+  L  DPP      P  ED+   Q  I GP  +PYA G+F + +    D+P  PPK
Sbjct:   14 RRVYKEVSTLTSDPPEGIKIIPNEEDITDVQVHIEGPEGTPYAAGIFRMKLILGKDFPAAPPK 76
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2L3_MOUSE (Ubiquitin-conjugating enzyme E2 L3 OS=Mus musculus GN=Ube2l3 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.328e-23
Identity = 32/82 (39.02%), Postives = 51/82 (62.20%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILK-EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKY 625
            PK  F+TK++HPNI+  G +CL ++  E W PA    +V+ S+ +L+ DP P+ PL  ++A  Y  D+ K+   A  +T+KY
Sbjct:   66 PKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKY 147

HSP 2 Score: 53.1434 bits (126), Expect = 1.328e-23
Identity = 27/67 (40.30%), Postives = 43/67 (64.18%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            AS+R++KEL++++K    +     V E ++  WQ  I+ P + PY  G F + I+FP +YPFKPPK+
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2L3_HUMAN (Ubiquitin-conjugating enzyme E2 L3 OS=Homo sapiens GN=UBE2L3 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.328e-23
Identity = 32/82 (39.02%), Postives = 51/82 (62.20%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILK-EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKY 625
            PK  F+TK++HPNI+  G +CL ++  E W PA    +V+ S+ +L+ DP P+ PL  ++A  Y  D+ K+   A  +T+KY
Sbjct:   66 PKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKY 147

HSP 2 Score: 53.1434 bits (126), Expect = 1.328e-23
Identity = 27/67 (40.30%), Postives = 43/67 (64.18%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            AS+R++KEL++++K    +     V E ++  WQ  I+ P + PY  G F + I+FP +YPFKPPK+
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2L3_BOVIN (Ubiquitin-conjugating enzyme E2 L3 OS=Bos taurus GN=UBE2L3 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.328e-23
Identity = 32/82 (39.02%), Postives = 51/82 (62.20%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILK-EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKY 625
            PK  F+TK++HPNI+  G +CL ++  E W PA    +V+ S+ +L+ DP P+ PL  ++A  Y  D+ K+   A  +T+KY
Sbjct:   66 PKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKY 147

HSP 2 Score: 53.1434 bits (126), Expect = 1.328e-23
Identity = 27/67 (40.30%), Postives = 43/67 (64.18%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            AS+R++KEL++++K    +     V E ++  WQ  I+ P + PY  G F + I+FP +YPFKPPK+
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2L3_PONAB (Ubiquitin-conjugating enzyme E2 L3 OS=Pongo abelii GN=UBE2L3 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.729e-23
Identity = 32/82 (39.02%), Postives = 51/82 (62.20%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILK-EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKY 625
            PK  F+TK++HPNI+  G +CL ++  E W PA    +V+ S+ +L+ DP P+ PL  ++A  Y  D+ K+   A  +T+KY
Sbjct:   66 PKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKY 147

HSP 2 Score: 52.7582 bits (125), Expect = 1.729e-23
Identity = 27/67 (40.30%), Postives = 43/67 (64.18%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            AS+R++KEL++++K    +     V E ++  WQ  I+ P + PY  G F + I+FP +YPFKPPK+
Sbjct:    3 ASRRLMKELEEIRKCGMKNFRNIRVDEANLLTWQGLIV-PDNPPYDKGAFRIEINFPAEYPFKPPKI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2C_DICDI (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum GN=ube2c PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.250e-23
Identity = 34/79 (43.04%), Postives = 51/79 (64.56%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKS--------DKVKYESTAR 607
            F T+ FHPN+++NG+ICLDILK++WSP   +  +L+SI SLL +PN + PL    A ++ +        DK   E+T+R
Sbjct:   74 FETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNESPLNSYAASLWSNQDEYKKVLDKRYQEATSR 152

HSP 2 Score: 58.151 bits (139), Expect = 2.250e-23
Identity = 27/65 (41.54%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  198 SKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            SKR+  EL +L   P    SA P  +++F+W  TI G  D+ Y    F +++ FP DYP+KPP V
Sbjct:    8 SKRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKFPTDYPYKPPTV 72
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC37_ARATH (Probable ubiquitin carrier protein E2 37 OS=Arabidopsis thaliana GN=UBC37 PE=2 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.864e-23
Identity = 41/88 (46.59%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNINSNGSICLDIL----KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            Q P   F T ++HPNI+++G ICLDIL    K  W P+L IS VL S+  LL++PNPDD L+ E++  YK ++  ++  AR  T+KYA
Sbjct:   77 QPPIVSFATPIYHPNIDNSGRICLDILNLPPKGAWQPSLNISTVLTSMRLLLSEPNPDDGLMCEVSREYKYNRQTFDYKAREMTEKYA 164

HSP 2 Score: 45.4394 bits (106), Expect = 4.864e-23
Identity = 30/75 (40.00%), Postives = 38/75 (50.67%), Query Frame = 3 
Query:  198 SKRILKELKDLQKDPPTSCS------AGPVAEDMFHWQ---ATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVA 395
            S R+ KELK L  DPP   S      A   + D   +    A I GP D+ YA G+F + I  P  YPF+PP V+
Sbjct:    8 SLRMQKELKLLLSDPPHGASFPHLSSAANGSGDFSTFSTIDAQIEGPEDTVYANGIFNLKIQIPERYPFQPPIVS 82
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC21_ARATH (Probable ubiquitin carrier protein E2 21 OS=Arabidopsis thaliana GN=UBC21 PE=1 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 6.447e-23
Identity = 37/87 (42.53%), Postives = 54/87 (62.07%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVK-YESTARSWTQKYAM 631
            Q P+  F TK+FHPN++   G ICLDILK  WSPA T+  V  +I +L+  P PD PL  +  ++ +S  V+ + S A+ +T+  AM
Sbjct:   67 QPPQVRFLTKIFHPNVHFKTGEICLDILKNAWSPAWTLQSVCRAIIALMAHPEPDSPLNCDSGNLLRSGDVRGFNSMAQMYTRLAAM 153

HSP 2 Score: 55.4546 bits (132), Expect = 6.447e-23
Identity = 25/65 (38.46%), Postives = 38/65 (58.46%), Query Frame = 3 
Query:  204 RILKELKDLQKDPPTSCSAGPVAED--MFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            R+ KE K++Q++         + +D  +F W A I GPS++PY GGVF +    P  YP +PP+V
Sbjct:    7 RLFKEYKEVQREKVADPDIQLICDDTNIFKWTALIKGPSETPYEGGVFQLAFSVPEPYPLQPPQV 71
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UBC7_YEAST7.830e-2450.70Ubiquitin-conjugating enzyme E2-18 kDa OS=Saccharo... [more]
UBC2_CRYNE1.019e-2346.27Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus ... [more]
UB2SC_XENLA1.319e-2342.70Ubiquitin-conjugating enzyme E2 S-C OS=Xenopus lae... [more]
UB2L3_MOUSE1.328e-2339.02Ubiquitin-conjugating enzyme E2 L3 OS=Mus musculus... [more]
UB2L3_HUMAN1.328e-2339.02Ubiquitin-conjugating enzyme E2 L3 OS=Homo sapiens... [more]
UB2L3_BOVIN1.328e-2339.02Ubiquitin-conjugating enzyme E2 L3 OS=Bos taurus G... [more]
UB2L3_PONAB1.729e-2339.02Ubiquitin-conjugating enzyme E2 L3 OS=Pongo abelii... [more]
UBE2C_DICDI2.250e-2343.04Probable ubiquitin-conjugating enzyme E2 C OS=Dict... [more]
UBC37_ARATH4.864e-2346.59Probable ubiquitin carrier protein E2 37 OS=Arabid... [more]
UBC21_ARATH6.447e-2342.53Probable ubiquitin carrier protein E2 21 OS=Arabid... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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