CK937528 | Citrus Genome Database

Overview


Name	CK937528
Unique Name	CK937528
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	712

Libraries


Library Name	Type
Developing fruit albedo at 80 DAFB in pTriplEx2 vector	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_DROER (Ubiquitin-conjugating enzyme E2 S OS=Drosophila erecta GN=GG18165 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 2.722e-20
Identity = 35/82 (42.68%), Postives = 46/82 (56.10%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK+FHPN+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   78 PKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 47.7506 bits (112), Expect = 2.722e-20
Identity = 22/63 (34.92%), Postives = 36/63 (57.14%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++++EL++++  PP          D+   QA I GP+ +PYA GVF V +    D+P  PPK
Sbjct:   17 RQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGVFRVKLTLNKDFPQTPPK 79

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC9B_DANRE (SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=ube2ib PE=1 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.741e-20
Identity = 34/84 (40.48%), Postives = 52/84 (61.90%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQ--WSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F   +FHPN+  +G++CL IL+E   W PA+TI ++LL I  LL +PN  DP   E   +Y  ++V+YE   R+  +K++
Sbjct:   73 PKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFS 156

HSP 2 Score: 45.8246 bits (107), Expect = 2.741e-20
Identity = 24/76 (31.58%), Postives = 37/76 (48.68%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAE-----DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVALGP 404
            +A  R+ +E K  +KD P    A P        ++ +W+  I G   +P+ GG+F + + F  DYP  PPK    P
Sbjct:    4 IALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEP 79

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_DROAN (Ubiquitin-conjugating enzyme E2 S OS=Drosophila ananassae GN=GF21161 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.540e-20
Identity = 35/82 (42.68%), Postives = 46/82 (56.10%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK+FHPN+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   78 PKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 47.3654 bits (111), Expect = 3.540e-20
Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++++EL++++  PP          D+   QA I GP+ +PYA G+F V +    D+P  PPK
Sbjct:   17 RQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAAGIFRVKLTLNKDFPQTPPK 79

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_DROWI (Ubiquitin-conjugating enzyme E2 S OS=Drosophila willistoni GN=GK16201 PE=3 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 4.606e-20
Identity = 35/82 (42.68%), Postives = 46/82 (56.10%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK+FHPN+ +NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   78 PKAYFLTKIFHPNVAANGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 46.9802 bits (110), Expect = 4.606e-20
Identity = 22/63 (34.92%), Postives = 36/63 (57.14%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++++EL++++  PP          D+   QA I GP+ +PYA GVF V +    D+P  PPK
Sbjct:   17 RQVMRELQEMENTPPEGIKVLINESDVTDIQALIDGPAGTPYAIGVFRVKLTLSKDFPQTPPK 79

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC20_ARATH (Ubiquitin carrier protein E2 20 OS=Arabidopsis thaliana GN=UBC20 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 6.011e-20
Identity = 30/67 (44.78%), Postives = 44/67 (65.67%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDK 583
            PK  F T  FHPN++  G+ICLDIL+++WS A  +  +LLSI SLL +PN   PL  + A ++ + +
Sbjct:   99 PKVKFETCCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQE 165

HSP 2 Score: 52.373 bits (124), Expect = 6.011e-20
Identity = 25/64 (39.06%), Postives = 36/64 (56.25%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            KR+  EL  L        SA P  +++F W+ TI G  D+ + G  + +++ F  DYPFKPPKV
Sbjct:   38 KRLQSELMGLMMGGGPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKV 101

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_DROPS (Ubiquitin-conjugating enzyme E2 S OS=Drosophila pseudoobscura pseudoobscura GN=GA22810 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 7.791e-20
Identity = 35/82 (42.68%), Postives = 45/82 (54.88%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK+FHPN+  NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   78 PKAYFLTKIFHPNVAGNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 46.595 bits (109), Expect = 7.791e-20
Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++++EL++++  PP          D+   QA I GP+ +PYA G+F V +    D+P  PPK
Sbjct:   17 RQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPK 79

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2S_DROPE (Ubiquitin-conjugating enzyme E2 S OS=Drosophila persimilis GN=GL16001 PE=3 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 7.791e-20
Identity = 35/82 (42.68%), Postives = 45/82 (54.88%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK  F TK+FHPN+  NG IC++ LK+ W P L I  +LL+I  LL  PNP+  L  E   M       Y   AR  T+ +A
Sbjct:   78 PKAYFLTKIFHPNVAGNGEICVNTLKKDWKPDLGIKHILLTIKCLLIVPNPESALNEEAGKMLLERYDDYSQRARMMTEIHA 159

HSP 2 Score: 46.595 bits (109), Expect = 7.791e-20
Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = 3

Query:  201 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPK 389
            +++++EL++++  PP          D+   QA I GP+ +PYA G+F V +    D+P  PPK
Sbjct:   17 RQVMRELQEMETTPPEGIKVLINESDVTDIQALIDGPAGTPYAVGIFRVKLTLSKDFPQTPPK 79

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC7_CAEEL (Probable ubiquitin-conjugating enzyme E2 7 OS=Caenorhabditis elegans GN=ubc-7 PE=1 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 1.326e-19
Identity = 27/67 (40.30%), Postives = 42/67 (62.69%), Query Frame = 3

Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            +S  + K+L D+++ P    SAG V + D++ W+  ++GP D+ Y GG F   + FP DYP KPPK+
Sbjct:    4 SSLLLKKQLADMRRVPVDGFSAGLVDDNDIYKWEVLVIGPPDTLYEGGFFKAILDFPRDYPQKPPKM 70

HSP 2 Score: 57.3806 bits (137), Expect = 1.326e-19
Identity = 27/84 (32.14%), Postives = 48/84 (57.14%), Query Frame = 2

Query:  383 PKGCFRTKVFHPNINSNGSICLDIL-------------KEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYE 595
            PK  F ++++HPNI+  G++C+ IL             +E+W P  T+  +LLS+ S+LTDPN + P   + A M + +  +++
Sbjct:   68 PKMKFISEIWHPNIDKEGNVCISILHDPGDDKWGYERPEERWLPVHTVETILLSVISMLTDPNFESPANVDAAKMQRENYAEFK 151

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC_ASFM2 (Ubiquitin-conjugating enzyme E2 OS=African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) GN=UBC PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.714e-19
Identity = 36/95 (37.89%), Postives = 53/95 (55.79%), Query Frame = 2

Query:  377 QAPKGCFRTKVFHPNINSNGSICLDIL-----KEQ---WSPALTISKVLLSICSLLTDPNPDDPLVPEIA-----HMYKSDKVKYESTARSWTQK 622
            + P+  F ++++HPNI S+G +C+ IL     +EQ   WSPA  I  VLLS+ SLL +PNPD P   + A     ++YK D   Y    +   +K
Sbjct:   63 EPPRLTFTSEMWHPNIYSDGKLCISILHGDNAEEQGMTWSPAQKIDTVLLSVISLLNEPNPDSPANVDAAKSYRKYLYKEDLESYPMEVKKTVKK 157

HSP 2 Score: 51.9878 bits (123), Expect = 1.714e-19
Identity = 23/67 (34.33%), Postives = 36/67 (53.73%), Query Frame = 3

Query:  192 MASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            M S  +L E K+L  +P          +++  W   + GP D+ Y GG+F   I FPP YP++PP++
Sbjct:    1 MVSSFLLAEYKNLIVNPSEHFKISVNEDNLTEWDVILKGPPDTLYEGGLFKAKIVFPPKYPYEPPRL 67

[Close]

BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBCX_DICDI (Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostelium discoideum GN=pex4 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.729e-19
Identity = 36/85 (42.35%), Postives = 51/85 (60.00%), Query Frame = 2

Query:  377 QAPKGCFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVK-YESTARSWTQKY 625
            Q P   F TK+FHPNI+   G ICLD+LK  WS   T+  V  SI +LL+ P  D PL  +  ++ ++  VK ++S AR +T+ Y
Sbjct:   63 QPPTIKFVTKIFHPNIHFKTGEICLDLLKTSWSAIYTLQSVCRSIIALLSLPEADSPLNCDAGNLIRNGDVKGHDSLARMYTRLY 147

HSP 2 Score: 50.0618 bits (118), Expect = 1.729e-19
Identity = 25/64 (39.06%), Postives = 35/64 (54.69%), Query Frame = 3

Query:  204 RILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            R++KE K LQ +        P  E D++ W A I GP D+PY  G F + I  P +YP +PP +
Sbjct:    4 RLMKEYKVLQNEEFEDILLYPRDESDLYRWVAIIKGPPDTPYENGKFELDISVPTNYPLQPPTI 67

The following BLAST results are available for this feature:

BLAST of CK937528 vs. ExPASy Swiss-Prot

BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246


Match Name	E-value	Identity	Description
UBE2S_DROER	2.722e-20	42.68	Ubiquitin-conjugating enzyme E2 S OS=Drosophila er...	[more]
UBC9B_DANRE	2.741e-20	40.48	SUMO-conjugating enzyme UBC9-B OS=Danio rerio GN=u...	[more]
UBE2S_DROAN	3.540e-20	42.68	Ubiquitin-conjugating enzyme E2 S OS=Drosophila an...	[more]
UBE2S_DROWI	4.606e-20	42.68	Ubiquitin-conjugating enzyme E2 S OS=Drosophila wi...	[more]
UBC20_ARATH	6.011e-20	44.78	Ubiquitin carrier protein E2 20 OS=Arabidopsis tha...	[more]
UBE2S_DROPS	7.791e-20	42.68	Ubiquitin-conjugating enzyme E2 S OS=Drosophila ps...	[more]
UBE2S_DROPE	7.791e-20	42.68	Ubiquitin-conjugating enzyme E2 S OS=Drosophila pe...	[more]
UBC7_CAEEL	1.326e-19	40.30	Probable ubiquitin-conjugating enzyme E2 7 OS=Caen...	[more]
UBC_ASFM2	1.714e-19	37.89	Ubiquitin-conjugating enzyme E2 OS=African swine f...	[more]
UBCX_DICDI	1.729e-19	42.35	Ubiquitin-conjugating enzyme E2 pex4 OS=Dictyostel...	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT