CK937528

Overview
NameCK937528
Unique NameCK937528
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length712
Libraries
Library NameType
Developing fruit albedo at 80 DAFB in pTriplEx2 vectorcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB12L_ARATH (Probable NEDD8-conjugating enzyme Ubc12-like OS=Arabidopsis thaliana GN=RCE2 PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 1.777e-14
Identity = 32/77 (41.56%), Postives = 49/77 (63.64%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTAR 607
            +APK   +TKV+HPNI+  G++CL+IL+E W P L I+ V+  +  L T+PN +DPL  E A + + +   +E   R
Sbjct:   92 EAPKVKCKTKVYHPNIDLEGNVCLNILREDWKPVLNINTVIYGLFHLFTEPNYEDPLNHEAAAVLRDNPKTFEYNVR 168

HSP 2 Score: 26.9498 bits (58), Expect = 1.777e-14
Identity = 16/63 (25.40%), Postives = 28/63 (44.44%), Query Frame = 3 
Query:  204 RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            R+ K++ +L        S      D+ +++ TI  P +  Y  G F+ +      YP + PKV
Sbjct:   35 RLHKDISELNLPKSCKISFPNGKNDLMNFEVTIK-PDEGYYLSGNFVFSFQVSNMYPHEAPKV 96
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2F_XENLA (NEDD8-conjugating enzyme UBE2F OS=Xenopus laevis GN=ube2f PE=2 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.777e-14
Identity = 33/89 (37.08%), Postives = 48/89 (53.93%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQ------WSPALTISKVLLSICSLLTD-PNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            PK    T+++HPNI   G ICL +L+E       W+P  T+  V+  + SL TD  N DDPL  E A  +  DK +Y +    + ++YA
Sbjct:   96 PKVKCLTRIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHLRDKDEYRNKVEDYIKRYA 184

HSP 2 Score: 39.2762 bits (90), Expect = 1.777e-14
Identity = 22/70 (31.43%), Postives = 33/70 (47.14%), Query Frame = 3 
Query:  186 RVMASKRIL-KELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            R+    R+L KE+ +L+ + P +C       +  H+    + P +S Y GG F   I  P  Y   PPKV
Sbjct:   29 RISVRDRLLVKEVAELEANLPCTCKVNFPDPNKLHYFHLTVSPDESYYQGGRFQFEIEVPDAYNMVPPKV 98
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC12_XENLA (NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis GN=ube2m PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.778e-14
Identity = 29/75 (38.67%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTAR 607
            PK    T V+HPNI+  G++CL+IL+E W P LTI+ ++  +  L  +PNP+DPL  E A + ++++  +E   +
Sbjct:   91 PKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165

HSP 2 Score: 28.8758 bits (63), Expect = 1.778e-14
Identity = 20/67 (29.85%), Postives = 32/67 (47.76%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAG-PVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A  RI K++ +L    P +C       +D+ +++  I  P +  Y GG F+ +      YP  PPKV
Sbjct:   30 AQLRIQKDITELNL--PKTCEIEFSDHDDLLNFKLVIC-PDEGFYKGGKFVFSFKVGQGYPHDPPKV 93
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2H_MOUSE (Ubiquitin-conjugating enzyme E2 H OS=Mus musculus GN=Ube2h PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.778e-14
Identity = 30/86 (34.88%), Postives = 50/86 (58.14%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            ++P   F  K+FHPNI+ ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   64 KSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 37.3502 bits (85), Expect = 1.778e-14
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3 
Query:  306 GPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            GP  +PY GGV+ V +  P  YPFK P +
Sbjct:   40 GPQGTPYEGGVWKVRVDLPDKYPFKSPSI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2H_HUMAN (Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens GN=UBE2H PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.778e-14
Identity = 30/86 (34.88%), Postives = 50/86 (58.14%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            ++P   F  K+FHPNI+ ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   64 KSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 37.3502 bits (85), Expect = 1.778e-14
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3 
Query:  306 GPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            GP  +PY GGV+ V +  P  YPFK P +
Sbjct:   40 GPQGTPYEGGVWKVRVDLPDKYPFKSPSI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBE2H_BOVIN (Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN=UBE2H PE=2 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.778e-14
Identity = 30/86 (34.88%), Postives = 50/86 (58.14%), Query Frame = 2 
Query:  377 QAPKGCFRTKVFHPNIN-SNGSICLDILKEQWSPALTISKVLLSIC-SLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYA 628
            ++P   F  K+FHPNI+ ++G++CLD++ + W+    ++ +  S    LL  PNP DPL  + A MY     +Y+   + + QKYA
Sbjct:   64 KSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYA 149

HSP 2 Score: 37.3502 bits (85), Expect = 1.778e-14
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3 
Query:  306 GPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            GP  +PY GGV+ V +  P  YPFK P +
Sbjct:   40 GPQGTPYEGGVWKVRVDLPDKYPFKSPSI 68
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC12_MOUSE (NEDD8-conjugating enzyme Ubc12 OS=Mus musculus GN=Ube2m PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.310e-14
Identity = 29/75 (38.67%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTAR 607
            PK    T V+HPNI+  G++CL+IL+E W P LTI+ ++  +  L  +PNP+DPL  E A + ++++  +E   +
Sbjct:   91 PKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165

HSP 2 Score: 28.4906 bits (62), Expect = 2.310e-14
Identity = 19/66 (28.79%), Postives = 31/66 (46.97%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A  RI K++ +L   P T   +    +D+ +++  I  P +  Y  G F+ +      YP  PPKV
Sbjct:   30 AQLRIQKDINELNL-PKTCDISFSDPDDLLNFKLVIC-PDEGFYKSGKFVFSFKVGQGYPHDPPKV 93
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC12_HUMAN (NEDD8-conjugating enzyme Ubc12 OS=Homo sapiens GN=UBE2M PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.310e-14
Identity = 29/75 (38.67%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTAR 607
            PK    T V+HPNI+  G++CL+IL+E W P LTI+ ++  +  L  +PNP+DPL  E A + ++++  +E   +
Sbjct:   91 PKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165

HSP 2 Score: 28.4906 bits (62), Expect = 2.310e-14
Identity = 19/66 (28.79%), Postives = 31/66 (46.97%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A  RI K++ +L   P T   +    +D+ +++  I  P +  Y  G F+ +      YP  PPKV
Sbjct:   30 AQLRIQKDINELNL-PKTCDISFSDPDDLLNFKLVIC-PDEGFYKSGKFVFSFKVGQGYPHDPPKV 93
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UBC12_BOVIN (NEDD8-conjugating enzyme Ubc12 OS=Bos taurus GN=UBE2M PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.310e-14
Identity = 29/75 (38.67%), Postives = 48/75 (64.00%), Query Frame = 2 
Query:  383 PKGCFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTAR 607
            PK    T V+HPNI+  G++CL+IL+E W P LTI+ ++  +  L  +PNP+DPL  E A + ++++  +E   +
Sbjct:   91 PKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQ 165

HSP 2 Score: 28.4906 bits (62), Expect = 2.310e-14
Identity = 19/66 (28.79%), Postives = 31/66 (46.97%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKV 392
            A  RI K++ +L   P T   +    +D+ +++  I  P +  Y  G F+ +      YP  PPKV
Sbjct:   30 AQLRIQKDINELNL-PKTCDISFSDPDDLLNFKLVIC-PDEGFYKSGKFVFSFKVGQGYPHDPPKV 93
[Close]
BLAST of CK937528 vs. ExPASy Swiss-Prot
Match: UB2L6_RAT (Ubiquitin/ISG15-conjugating enzyme E2 L6 OS=Rattus norvegicus GN=Ube2l6 PE=2 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 3.018e-14
Identity = 25/80 (31.25%), Postives = 46/80 (57.50%), Query Frame = 2 
Query:  395 FRTKVFHPNINSNGSICLDILK-EQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKSDKVKYESTARSWTQKYAM 631
            F TK++HPNI+ +G +CL ++  E W P     +VL ++  L++ PN ++P+  E+A +   D   +   A  +T +Y +
Sbjct:   70 FTTKIYHPNISEDGLVCLPLISTENWKPYTKAYQVLEALNILVSRPNLEEPVRLELADLLTQDPEMFRKKAEEFTLQYGV 149

HSP 2 Score: 42.743 bits (99), Expect = 3.018e-14
Identity = 25/65 (38.46%), Postives = 33/65 (50.77%), Query Frame = 3 
Query:  195 ASKRILKELKDLQKD-PPTSCSAGPVAEDMFHWQATIMGPSDSPYAGGVFLVTIHFPPDYPFKPP 386
            ASKR+ KEL DL K+ PP          ++  W   ++ P   PY    F + I FP +YP KPP
Sbjct:    3 ASKRVAKELDDLSKELPPYLRHLSSDDANVLVWHMLLL-PDQLPYRLKAFGLRIDFPREYPLKPP 66
The following BLAST results are available for this feature:
BLAST of CK937528 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
UB12L_ARATH1.777e-1441.56Probable NEDD8-conjugating enzyme Ubc12-like OS=Ar... [more]
UBE2F_XENLA1.777e-1437.08NEDD8-conjugating enzyme UBE2F OS=Xenopus laevis G... [more]
UBC12_XENLA1.778e-1438.67NEDD8-conjugating enzyme Ubc12 OS=Xenopus laevis G... [more]
UBE2H_MOUSE1.778e-1434.88Ubiquitin-conjugating enzyme E2 H OS=Mus musculus ... [more]
UBE2H_HUMAN1.778e-1434.88Ubiquitin-conjugating enzyme E2 H OS=Homo sapiens ... [more]
UBE2H_BOVIN1.778e-1434.88Ubiquitin-conjugating enzyme E2 H OS=Bos taurus GN... [more]
UBC12_MOUSE2.310e-1438.67NEDD8-conjugating enzyme Ubc12 OS=Mus musculus GN=... [more]
UBC12_HUMAN2.310e-1438.67NEDD8-conjugating enzyme Ubc12 OS=Homo sapiens GN=... [more]
UBC12_BOVIN2.310e-1438.67NEDD8-conjugating enzyme Ubc12 OS=Bos taurus GN=UB... [more]
UB2L6_RAT3.018e-1431.25Ubiquitin/ISG15-conjugating enzyme E2 L6 OS=Rattus... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004779_F05 Developing fruit albedo at 80 DAFB in pTriplEx2 vector Citrus sinensis cDNA clone A80DAB0003_IIIF_F05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK937528 ID=CK937528; Name=CK937528; organism=Citrus sinensis; type=EST; length=712bp
GGGGGACGATATTTGTGTTGAAGAACAACGAAGCAAGGGGTTGATAGAGG
AGCGAGGCGGGGGCGGCTTGACGACCTAGATCTGCTTCACCGTTTCGCTT
TCTCGACGCTATCGCTCTCCGATCCTGTTCTCTGCCGCGCAAGGTCTTAA
GGTTCGTTGTAGTTTCAATTTTAATAATCCCCTGAAGAGTCATGGCTTCA
AAGCGTATTTTGAAGGAACTCAAGGATTTGCAGAAGGATCCTCCCACGTC
TTGCAGCGCTGGCCCTGTGGCTGAAGACATGTTCCACTGGCAAGCAACAA
TTATGGGCCCTTCTGATAGCCCATATGCAGGAGGTGTATTTTTAGTTACC
ATTCATTTTCCTCCTGATTATCCTTTCAAGCCCCCAAAGGTTGCTTTAGG
ACCAAGGTCTTCCACCCGAACATCAACAGCAATGGAAGCATCTGTCTTGA
TATCCTTAAAGAACAGTGGAGTCCAGCGCTTACCATTTCAAAGGTCCTGC
TCTCTATCTGCTCCTTGCTTACTGATCCAAATCCTGACGACCCTCTTGTA
CCGGAGATTGCCCACATGTACAAGTCCGACAAGGTCAAATATGAATCCAC
GGCCAGGAGCTGGACACAGAAGTATGCCATGGGATGAAGGTTGTTAGGAT
ATTGCAGAANNNGGAACACATTTATTGAAATATGTTGTGTGNNNTGTCTA
TCTATTTTCTTT
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