CK936872

Overview
NameCK936872
Unique NameCK936872
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOM_MOUSE (NAD-dependent malic enzyme, mitochondrial OS=Mus musculus GN=Me2 PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 1.250e-38
Identity = 70/142 (49.30%), Postives = 107/142 (75.35%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            G  L+ +PR NKG+AFT +ER    L+GLLPP + +Q +Q  +   ++++   PL+KY+ +M ++ERNE+LFY++L D++E L+P+VYTPTVG AC +YG IFRRP+GL+IS+ ++G +  ++ NWPE  ++ +VVTDGERI
Sbjct:   25 GKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACCQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERI 166

HSP 2 Score: 20.4014 bits (41), Expect = 1.250e-38
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA  GI+P
Sbjct:  181 VGKLCLYTACAGIQP 195
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOX_ANAPL (NADP-dependent malic enzyme OS=Anas platyrhynchos GN=ME1 PE=1 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.251e-38
Identity = 71/142 (50.00%), Postives = 102/142 (71.83%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY +LRDP  NKG+AFT +ER    + GLLPP  + Q +Q   ++ +  +    L +Y+ +M L++RNE+LFYK+L  ++E  +P+VYTPTVG ACQ+YG  FRRP+GL+I++ ++G I  +LK+WPE  I+ IVVTDGERI
Sbjct:    4 GYEVLRDPHLNKGMAFTLEERQQLNIHGLLPPCFLGQDVQVFSILKNFERLTSDLDRYILLMSLQDRNEKLFYKVLTSDIERFMPIVYTPTVGLACQQYGLAFRRPRGLFITIHDRGHIATMLKSWPESVIKAIVVTDGERI 145

HSP 2 Score: 22.7126 bits (47), Expect = 1.251e-38
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG++P
Sbjct:  160 VGKLALYTACGGVKP 174
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOX_MOUSE (NADP-dependent malic enzyme OS=Mus musculus GN=Me1 PE=1 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 1.331e-38
Identity = 72/142 (50.70%), Postives = 102/142 (71.83%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY L RDP  NK LAFT +ER    + GLLPP +ISQ+LQ  +++ +  +      +Y+ +M+L++RNE+LFY +L+ +VE+ +P+VYTPTVG ACQ+Y   FR+P+GL+IS+ +KG I  VL  WPE  ++ IVVTDGERI
Sbjct:   15 GYLLTRDPHLNKDLAFTLEERQQLNIHGLLPPCIISQELQVLRIIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERI 156

HSP 2 Score: 21.9422 bits (45), Expect = 1.331e-38
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG+ P
Sbjct:  171 VGKLALYTACGGVNP 185

HSP 3 Score: 21.1718 bits (43), Expect = 1.331e-38
Identity = 10/17 (58.82%), Postives = 10/17 (58.82%), Query Frame = 3 
Query:  654 LGILAXRGWEFPVGKLA 704
            LG L   G   PVGKLA
Sbjct:  159 LGDLGCNGMGIPVGKLA 175
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOX_RAT (NADP-dependent malic enzyme OS=Rattus norvegicus GN=Me1 PE=1 SV=2)

HSP 1 Score: 155.992 bits (393), Expect = 2.249e-38
Identity = 70/142 (49.30%), Postives = 102/142 (71.83%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY L RDP  NK LAFT +ER    + GLLPP +++Q++Q  +++ +  +      +Y+ +M+L++RNE+LFY +L+ NVE+ +P+VYTPTVG ACQ+Y   FR+P+GL+IS+ +KG I  VL  WPE  ++ IVVTDGERI
Sbjct:   15 GYLLTRDPHLNKDLAFTLEERQQLKIHGLLPPCIVNQEIQVLRVIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSNVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERI 156

HSP 2 Score: 21.9422 bits (45), Expect = 2.249e-38
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG+ P
Sbjct:  171 VGKLALYTACGGVNP 185

HSP 3 Score: 21.1718 bits (43), Expect = 2.249e-38
Identity = 10/17 (58.82%), Postives = 10/17 (58.82%), Query Frame = 3 
Query:  654 LGILAXRGWEFPVGKLA 704
            LG L   G   PVGKLA
Sbjct:  159 LGDLGCNGMGIPVGKLA 175
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOX_COLLI (NADP-dependent malic enzyme OS=Columba livia GN=ME1 PE=1 SV=1)

HSP 1 Score: 154.066 bits (388), Expect = 7.965e-38
Identity = 70/142 (49.30%), Postives = 100/142 (70.42%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY +LRDP  NKG+AFT +ER    + GLLPP  + Q  Q   ++ +  +    L +Y+ +M L++RNE+LFYK+L  ++E  +P+VYTPTVG ACQ YG  FRRP+GL+I++ ++G I  +L++WPE  I+ IVVTDGERI
Sbjct:    4 GYEVLRDPHLNKGMAFTLEERQQLNIHGLLPPCFLGQDAQVYSILKNFERLTSDLDRYILLMSLQDRNEKLFYKVLTSDIERFMPIVYTPTVGLACQHYGLAFRRPRGLFITIHDRGHIATMLQSWPESVIKAIVVTDGERI 145

HSP 2 Score: 22.7126 bits (47), Expect = 7.965e-38
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG++P
Sbjct:  160 VGKLALYTACGGVKP 174
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAON_HUMAN (NADP-dependent malic enzyme, mitochondrial OS=Homo sapiens GN=ME3 PE=2 SV=2)

HSP 1 Score: 152.14 bits (383), Expect = 5.066e-37
Identity = 69/142 (48.59%), Postives = 103/142 (72.54%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY + R+P  NKG+AFT +ER    + GL+PP  +SQ +Q  ++M    + +  L KY+ +M L++RNE+LFY++L  +VE+ +P+VYTPTVG ACQ YG  FRRP+GL+I++ +KG +  +L +WPE +I+ +VVTDGERI
Sbjct:   50 GYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNIKAVVVTDGERI 191

HSP 2 Score: 21.9422 bits (45), Expect = 5.066e-37
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG+ P
Sbjct:  206 VGKLALYTACGGVNP 220
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAON_MOUSE (NADP-dependent malic enzyme, mitochondrial OS=Mus musculus GN=Me3 PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 2.475e-36
Identity = 69/142 (48.59%), Postives = 101/142 (71.13%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY + R+P  NKG+AFT +ER    + GL+PP  +SQ +Q  ++M      +  L KY+ +M  ++RNE+LFY++L  +VE+ +P+VYTPTVG ACQ YG  FRRP+GL+I++ +KG I  +L +WPE +I+ +VVTDGERI
Sbjct:   50 GYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYENQQSDLDKYIILMTHQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHIATMLNSWPEDNIKAVVVTDGERI 191

HSP 2 Score: 21.9422 bits (45), Expect = 2.475e-36
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG+ P
Sbjct:  206 VGKLALYTACGGVNP 220
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOX_PIG (NADP-dependent malic enzyme (Fragment) OS=Sus scrofa GN=ME1 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 2.520e-36
Identity = 68/142 (47.89%), Postives = 100/142 (70.42%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY L R P  NK LAFT +ER    + GLLPP  ISQ +Q  +++ +  +      +Y+ +M+L++RNE+LFYK+L+ ++E+ +P+VYTPTVG ACQ+Y   FR+P+GL+IS+ ++G +  VL  WPE  I+ +VVTDGERI
Sbjct:    1 GYGLTRIPHLNKDLAFTLEERQQLNIHGLLPPCFISQDIQVLRVIKNFERLNSDFDRYLLLMDLQDRNEKLFYKVLMSDIEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDRGHVASVLNAWPEDVIKAVVVTDGERI 142

HSP 2 Score: 21.9422 bits (45), Expect = 2.520e-36
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG+ P
Sbjct:  157 VGKLALYTACGGVNP 171

HSP 3 Score: 21.1718 bits (43), Expect = 2.520e-36
Identity = 10/17 (58.82%), Postives = 10/17 (58.82%), Query Frame = 3 
Query:  654 LGILAXRGWEFPVGKLA 704
            LG L   G   PVGKLA
Sbjct:  145 LGDLGCNGMGIPVGKLA 161
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOX_HUMAN (NADP-dependent malic enzyme OS=Homo sapiens GN=ME1 PE=1 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 5.527e-36
Identity = 67/142 (47.18%), Postives = 101/142 (71.13%), Query Frame = 2 
Query:  221 GYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            GY L R+P  NK LAFT +ER    + GLLPP+  SQ++Q  +++ +         +Y+ +M+L++RNE+LFY++L  ++E+ +P+VYTPTVG ACQ+Y  +FR+P+GL+I++ ++G I  VL  WPE  I+ IVVTDGERI
Sbjct:   15 GYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERI 156

HSP 2 Score: 21.1718 bits (43), Expect = 5.527e-36
Identity = 10/17 (58.82%), Postives = 10/17 (58.82%), Query Frame = 3 
Query:  654 LGILAXRGWEFPVGKLA 704
            LG L   G   PVGKLA
Sbjct:  159 LGDLGCNGMGIPVGKLA 175

HSP 3 Score: 21.1718 bits (43), Expect = 5.527e-36
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GG+ P
Sbjct:  171 VGKLALYTACGGMNP 185
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAOM_ASCSU (NAD-dependent malic enzyme, mitochondrial (Fragment) OS=Ascaris suum PE=1 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 4.491e-34
Identity = 67/141 (47.52%), Postives = 103/141 (73.05%), Query Frame = 2 
Query:  230 LLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEK--GKILEVLKNWPERSIQVIVVTDGERI 646
            LL++PR NKG+ F+  ER    L GLLPPA ++Q+ Q  +++  +R+    L +Y+ +  L++RNE+LFY+++ D+V+EL+P+VYTPTVG ACQ +G I+R+P+GLYI++ +    KI ++L NW E  ++ IVVTDGERI
Sbjct:   80 LLKEPRLNKGMGFSLYERQYLGLHGLLPPAFMTQEQQAYRVITKLREQPNDLARYIQLDGLQDRNEKLFYRVVCDHVKELMPIVYTPTVGLACQNFGYIYRKPKGLYITINDNSVSKIYQILSNWHEEDVRAIVVTDGERI 220
The following BLAST results are available for this feature:
BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAOM_MOUSE1.250e-3849.30NAD-dependent malic enzyme, mitochondrial OS=Mus m... [more]
MAOX_ANAPL1.251e-3850.00NADP-dependent malic enzyme OS=Anas platyrhynchos ... [more]
MAOX_MOUSE1.331e-3850.70NADP-dependent malic enzyme OS=Mus musculus GN=Me1... [more]
MAOX_RAT2.249e-3849.30NADP-dependent malic enzyme OS=Rattus norvegicus G... [more]
MAOX_COLLI7.965e-3849.30NADP-dependent malic enzyme OS=Columba livia GN=ME... [more]
MAON_HUMAN5.066e-3748.59NADP-dependent malic enzyme, mitochondrial OS=Homo... [more]
MAON_MOUSE2.475e-3648.59NADP-dependent malic enzyme, mitochondrial OS=Mus ... [more]
MAOX_PIG2.520e-3647.89NADP-dependent malic enzyme (Fragment) OS=Sus scro... [more]
MAOX_HUMAN5.527e-3647.18NADP-dependent malic enzyme OS=Homo sapiens GN=ME1... [more]
MAOM_ASCSU4.491e-3447.52NAD-dependent malic enzyme, mitochondrial (Fragmen... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC
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