CK936872 | Citrus Genome Database

Overview


Name	CK936872
Unique Name	CK936872
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	735

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOL5 (NAD-dependent malic enzyme OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOK1 (NAD-dependent malic enzyme OS=Escherichia coli O1:K1 / APEC GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOHS (NAD-dependent malic enzyme OS=Escherichia coli O9:H4 (strain HS) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECODH (NAD-dependent malic enzyme OS=Escherichia coli (strain K12 / DH10B) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOBW (NAD-dependent malic enzyme OS=Escherichia coli (strain K12 / BW2952) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO8A (NAD-dependent malic enzyme OS=Escherichia coli O8 (strain IAI1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO81 (NAD-dependent malic enzyme OS=Escherichia coli O81 (strain ED1a) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO7I (NAD-dependent malic enzyme OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO5E (NAD-dependent malic enzyme OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO57 (NAD-dependent malic enzyme OS=Escherichia coli O157:H7 GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

The following BLAST results are available for this feature:

BLAST of CK936872 vs. ExPASy Swiss-Prot

BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
MAO1_ECOL5	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O6:...	[more]
MAO1_ECOK1	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O1:...	[more]
MAO1_ECOHS	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O9:...	[more]
MAO1_ECODH	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOBW	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECO8A	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O8 ...	[more]
MAO1_ECO81	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O81...	[more]
MAO1_ECO7I	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O7:...	[more]
MAO1_ECO5E	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O15...	[more]
MAO1_ECO57	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O15...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC