CK936872 | Citrus Genome Database

Overview


Name	CK936872
Unique Name	CK936872
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	735

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO55 (NAD-dependent malic enzyme OS=Escherichia coli (strain 55989 / EAEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO45 (NAD-dependent malic enzyme OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO27 (NAD-dependent malic enzyme OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECO24 (NAD-dependent malic enzyme OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHESR (NAD-dependent malic enzyme OS=Shewanella sp. (strain MR-7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.063e-26
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 1.063e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHESM (NAD-dependent malic enzyme OS=Shewanella sp. (strain MR-4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.063e-26
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 1.063e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHESA (NAD-dependent malic enzyme OS=Shewanella sp. (strain ANA-3) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.063e-26
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 1.063e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEON (NAD-dependent malic enzyme OS=Shewanella oneidensis GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 1.063e-26
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 1.063e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEHH (NAD-dependent malic enzyme OS=Shewanella halifaxensis (strain HAW-EB4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.344e-26
Identity = 54/143 (37.76%), Postives = 93/143 (65.03%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFTE+ER    L GLLP  + + + Q  +  +  + +   L K++ +  +++ NE L+Y+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTEEERIFFNLEGLLPHVIETIEEQASRAYDQYKNFSNDLDKHIYLRNIQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKDYRRNRGLFISYANKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.344e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

[Close]

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PSEU2 (NAD-dependent malic enzyme OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sfcA PE=3 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 3.051e-26
Identity = 62/150 (41.33%), Postives = 98/150 (65.33%), Query Frame = 2

Query:  197 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            P  IS A G  LL  P  NKG AFT +ER    L GLLP  V + + Q  ++ +  +Q    L K++ +  +++ NE LF++LL  +++E+LP++YTPTVG+ACQ++  I+R  +GL+IS  E+ +I ++L++  +  I++IVVTD ERI
Sbjct:    7 PLYISYA-GPSLLEMPLLNKGSAFTPQERVEFNLIGLLPQNVETIEEQVTRVYSQYKQCASDLDKHIYLRSIQDNNETLFFRLLDSHLDEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPERDRIDDILRSATKDRIKIIVVTDSERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 3.051e-26
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184

The following BLAST results are available for this feature:

BLAST of CK936872 vs. ExPASy Swiss-Prot

BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
MAO1_ECO55	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECO45	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O45...	[more]
MAO1_ECO27	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O12...	[more]
MAO1_ECO24	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O13...	[more]
MAO1_SHESR	1.063e-26	38.46	NAD-dependent malic enzyme OS=Shewanella sp. (stra...	[more]
MAO1_SHESM	1.063e-26	38.46	NAD-dependent malic enzyme OS=Shewanella sp. (stra...	[more]
MAO1_SHESA	1.063e-26	38.46	NAD-dependent malic enzyme OS=Shewanella sp. (stra...	[more]
MAO1_SHEON	1.063e-26	38.46	NAD-dependent malic enzyme OS=Shewanella oneidensi...	[more]
MAO1_SHEHH	2.344e-26	37.76	NAD-dependent malic enzyme OS=Shewanella halifaxen...	[more]
MAO1_PSEU2	3.051e-26	41.33	NAD-dependent malic enzyme OS=Pseudomonas syringae...	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC