CK936872

Overview
NameCK936872
Unique NameCK936872
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PSESM (NAD-dependent malic enzyme OS=Pseudomonas syringae pv. tomato GN=sfcA PE=3 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 3.051e-26
Identity = 62/150 (41.33%), Postives = 98/150 (65.33%), Query Frame = 2 
Query:  197 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            P  IS A G  LL  P  NKG AFT +ER    L GLLP  V + + Q  ++ +  +Q    L K++ +  +++ NE LF++LL  +++E+LP++YTPTVG+ACQ++  I+R  +GL+IS  E+ +I ++L++  +  I++IVVTD ERI
Sbjct:    7 PLYISYA-GPSLLEMPLLNKGSAFTPQERIEFNLIGLLPQNVETIEEQVTRVYSQYKQCASDLDKHIYLRSIQDNNETLFFRLLDSHLDEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPERDRIDDILRSATKDRIKIIVVTDSERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 3.051e-26
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PSE14 (NAD-dependent malic enzyme OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sfcA PE=3 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 3.051e-26
Identity = 62/150 (41.33%), Postives = 98/150 (65.33%), Query Frame = 2 
Query:  197 PWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            P  IS A G  LL  P  NKG AFT +ER    L GLLP  V + + Q  ++ +  +Q    L K++ +  +++ NE LF++LL  +++E+LP++YTPTVG+ACQ++  I+R  +GL+IS  E+ +I ++L++  +  I++IVVTD ERI
Sbjct:    7 PLYISYA-GPSLLEMPLLNKGSAFTPQERVEFNLIGLLPQNVETIEEQVTRVYSQYKQCASDLDKHIYLRSIQDNNETLFFRLLDSHLDEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPERDRIDDILRSATKDRIKIIVVTDSERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 3.051e-26
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPA (NAD-dependent malic enzyme OS=Shewanella pealeana (strain ATCC 700345 / ANG-SQ1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 5.169e-26
Identity = 53/143 (37.06%), Postives = 93/143 (65.03%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFT++ER    L GLLP  + + + Q  +  +  + +   L K++ +  +++ NE L+Y+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTDEERIFFNLEGLLPHVIETIEEQASRAYDQYKNFSNDLDKHIYLRNIQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKDYRRNRGLFISYANKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 5.169e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHELP (NAD-dependent malic enzyme OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=sfcA PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 5.169e-26
Identity = 54/143 (37.76%), Postives = 93/143 (65.03%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFTE+ER    L GLLP  + + + Q  +  +  + +   L +++ +  +++ NE L+Y+LL +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTEEERIFFNLEGLLPHVIETIEEQASRAYDQYKNFGNDLDRHIYLRNIQDTNETLYYRLLQNHITEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 5.169e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEDO (NAD-dependent malic enzyme OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) GN=sfcA PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 5.169e-26
Identity = 56/143 (39.16%), Postives = 90/143 (62.94%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GLLP  + + + Q  +       +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N     ++VIVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLLPYVIETIEEQAARAYAQFSNFSNDLDKHIYLRNIQDTNETLFYRLVRNNITEMMPIIYTPTVGLACERFSKNYRRNRGLFISYSNKDRIDDILNNSTRHKVKVIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 5.169e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPW (NAD-dependent malic enzyme OS=Shewanella piezotolerans (strain WP3 / JCM 13877) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.728e-26
Identity = 53/143 (37.06%), Postives = 93/143 (65.03%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFT++ER    L GLLP  + + + Q  +  +  + +   L K++ +  +++ NE L+Y+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTDEERIFFNLEGLLPHVIETIEEQASRAYDQYKNFTNDLDKHIYLRNIQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKDYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 6.728e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEFN (NAD-dependent malic enzyme OS=Shewanella frigidimarina (strain NCIMB 400) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 6.728e-26
Identity = 55/143 (38.46%), Postives = 90/143 (62.94%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P  + +   Q  +     + +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N     ++VIVVTDGERI
Sbjct:   13 AGPAILESPLLNKGSAFSEEERIYFNLEGLIPWVIETIDEQAARAYAQFKNFTNDLDKHIYLRNIQDTNETLFYRLVRNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRHKVKVIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 6.728e-26
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEWM (NAD-dependent malic enzyme OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 2.513e-25
Identity = 52/143 (36.36%), Postives = 92/143 (64.34%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFT++ER    L GLLP  + + + Q  +  +    +   L +++ +  +++ NE L+Y+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTDEERVFFNLEGLLPHVIETIEEQASRAYDQYTNFTNDLDRHIYLRNIQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKNYRRNRGLFISYPHKDRIEDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.513e-25
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHESH (NAD-dependent malic enzyme OS=Shewanella sediminis (strain HAW-EB3) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 2.513e-25
Identity = 53/143 (37.06%), Postives = 92/143 (64.34%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AFT++ER    L GLLP  + + + Q  +  +    +   L K++ +  +++ NE L+Y+L+ +++ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFTDEERVFFNLEGLLPHVIETIEEQASRAYDQYTNFTNDLDKHIYLRNIQDTNETLYYRLVQNHITEMMPIIYTPTVGMACERFSKNYRRNRGLFISYPNKDRIDDLLNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.513e-25
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PSEA7 (NAD-dependent malic enzyme OS=Pseudomonas aeruginosa (strain PA7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 3.270e-25
Identity = 52/143 (36.36%), Postives = 96/143 (67.13%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L+GLLP  + + + Q ++  +        L +++ +  +++ NE LF++LL +++EE+LP++YTPTVG+ACQ++  I+R  +GL+IS  ++ +I ++L++  + +++++VVTD ERI
Sbjct:   14 AGPSLLEMPLLNKGSAFSTQERIDFNLQGLLPHNIETIEEQTERAYSQYNLCNTDLDRHIFLRSIQDNNETLFFRLLEEHLEEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVTDSERI 156

HSP 2 Score: 21.9422 bits (45), Expect = 3.270e-25
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  171 IGKLSLYTACGGISP 185
The following BLAST results are available for this feature:
BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_PSESM3.051e-2641.33NAD-dependent malic enzyme OS=Pseudomonas syringae... [more]
MAO1_PSE143.051e-2641.33NAD-dependent malic enzyme OS=Pseudomonas syringae... [more]
MAO1_SHEPA5.169e-2637.06NAD-dependent malic enzyme OS=Shewanella pealeana ... [more]
MAO1_SHELP5.169e-2637.76NAD-dependent malic enzyme OS=Shewanella loihica (... [more]
MAO1_SHEDO5.169e-2639.16NAD-dependent malic enzyme OS=Shewanella denitrifi... [more]
MAO1_SHEPW6.728e-2637.06NAD-dependent malic enzyme OS=Shewanella piezotole... [more]
MAO1_SHEFN6.728e-2638.46NAD-dependent malic enzyme OS=Shewanella frigidima... [more]
MAO1_SHEWM2.513e-2536.36NAD-dependent malic enzyme OS=Shewanella woodyi (s... [more]
MAO1_SHESH2.513e-2537.06NAD-dependent malic enzyme OS=Shewanella sediminis... [more]
MAO1_PSEA73.270e-2536.36NAD-dependent malic enzyme OS=Pseudomonas aerugino... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC
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