CK936872

Overview
NameCK936872
Unique NameCK936872
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PECCP (NAD-dependent malic enzyme OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.209e-29
Identity = 61/143 (42.66%), Postives = 99/143 (69.23%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFTE+ER    L GLLP AV + + Q ++     ++++  ++K+V +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERANFNLHGLLPEAVETIEEQAERAWRQYQEFKHDIEKHVYLRNIQDTNETLFYRLLDGHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRAHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.209e-29
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_AERS4 (NAD-dependent malic enzyme OS=Aeromonas salmonicida (strain A449) GN=sfcA PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.209e-29
Identity = 64/161 (39.75%), Postives = 105/161 (65.22%), Query Frame = 2 
Query:  164 EDRATEDQLVTPWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            ED   +  L  P+     +G  LL  P  NKG AFT +ER +  L GLLP  + + + Q ++       +   L K++ +  +++ NE LFY+LL +++ E+LPV+YTPTVG+AC+++ +I+RR +GL+IS  +K +I ++L+N  +++++VIVVTDGERI
Sbjct:    3 EDNNQKRPLYIPY-----AGPALLETPLLNKGCAFTSEERSSFNLEGLLPQNIETIEEQAERAYRQFMAFGNDLDKHIYLRNIQDTNETLFYRLLHNHLTEMLPVIYTPTVGKACEEFSNIYRRARGLFISYPDKDRIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.209e-29
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SERP5 (NAD-dependent malic enzyme OS=Serratia proteamaculans (strain 568) GN=sfcA PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 5.440e-29
Identity = 60/143 (41.96%), Postives = 99/143 (69.23%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFTE+ER+   L+GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   + +I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERNHFNLQGLLPDAVETIEEQAERAYRQYQDFKNDSDKHIYLRNIQDTNETLFYRLLDSHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRDRIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 5.440e-29
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_AERHH (NAD-dependent malic enzyme OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=sfcA PE=3 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 9.220e-29
Identity = 63/161 (39.13%), Postives = 104/161 (64.60%), Query Frame = 2 
Query:  164 EDRATEDQLVTPWTISVASGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            ED   +  L  P+     +G  LL  P  NKG AFT +ER    L GLLP  + + + Q ++       +   + K++ +  +++ NE LFY+LL +++ E+LPV+YTPTVG+AC+++ +I+RR +GL+IS  +K +I ++L+N  +++++VIVVTDGERI
Sbjct:    3 EDNNQKRPLYIPY-----AGPALLETPLLNKGSAFTSEERSNFNLEGLLPQNIETIEEQAERAYRQFMAFGNDMDKHIYLRNIQDTNETLFYRLLTNHLTEMLPVIYTPTVGKACEEFSNIYRRARGLFISYPDKDRIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 9.220e-29
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO12_PHOPR (NAD-dependent malic enzyme 2 OS=Photobacterium profundum GN=sfcA2 PE=3 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 9.221e-29
Identity = 54/143 (37.76%), Postives = 98/143 (68.53%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL +   NKG AF+ +ER    L GLLP A+ S   QE++  +  + +   + K++ +  +++ NE L+Y+L+ +++EE++P++YTPTVG AC+++ +I+RR +GL++   +KG I+++L N   + +++IV+TDGERI
Sbjct:   13 AGPTLLENALLNKGSAFSPEERQNFSLMGLLPAAIESITEQEERAYSQYQTFNDDMDKHIYLRNIQDTNETLYYRLIDNHIEEMMPIIYTPTVGAACEQFSNIYRRGRGLFLGYPDKGNIVDILNNAARQDVKIIVITDGERI 155

HSP 2 Score: 22.7126 bits (47), Expect = 9.221e-29
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLALYTACGGINP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBFM (NAD-dependent malic enzyme OS=Vibrio fischeri (strain MJ11) GN=sfcA PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 2.649e-28
Identity = 58/143 (40.56%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+  ER    L GLLP A+ S + Q  +     + +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS + K +I ++L N   ++++VIVVTDGERI
Sbjct:   13 AGPALLSTPLLNKGSAFSTTERKYFNLEGLLPEAIESIEEQTGRAYKQYQSFENDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYENKNRIDDLLNNAANQNVKVIVVTDGERI 155

HSP 2 Score: 22.3274 bits (46), Expect = 2.649e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLALYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBF1 (NAD-dependent malic enzyme OS=Vibrio fischeri (strain ATCC 700601 / ES114) GN=sfcA PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 2.649e-28
Identity = 58/143 (40.56%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+  ER    L GLLP A+ S + Q  +     + +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS + K +I ++L N   ++++VIVVTDGERI
Sbjct:   13 AGPALLSTPLLNKGSAFSTTERKYFNLEGLLPEAIESIEEQTGRAYKQYQNFENDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYENKNRIDDLLNNAANQNVKVIVVTDGERI 155

HSP 2 Score: 22.3274 bits (46), Expect = 2.649e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLALYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ALISL (NAD-dependent malic enzyme OS=Aliivibrio salmonicida (strain LFI1238) GN=sfcA PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 2.649e-28
Identity = 58/143 (40.56%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+  ER    L GLLP A+ S + Q  +     + +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS + K +I ++L N   ++++VIVVTDGERI
Sbjct:   13 AGPALLSTPLLNKGSAFSTTERKYFNLEGLLPEAIESIEEQTGRAYKQYQSFENDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYENKNRIDDLLNNAANQNVKVIVVTDGERI 155

HSP 2 Score: 22.3274 bits (46), Expect = 2.649e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLALYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_PHOLL (NAD-dependent malic enzyme OS=Photorhabdus luminescens subsp. laumondii GN=sfcA PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 5.844e-28
Identity = 62/143 (43.36%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            SG  LL  P  NKG AFTE+ER    L GLLP AV + + Q ++       ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   K  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 SGPILLEFPLLNKGSAFTEEERRNFNLHGLLPEAVETIEEQAERAYRQYLDFKNDADKHIYLRNIQDTNETLFYRLLDAHLNEMMPIIYTPTVGEACEHFSDIYRRARGLFISHPNKAHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 20.7866 bits (42), Expect = 5.844e-28
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  173 IGKLSLYTSCGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ENT38 (NAD-dependent malic enzyme OS=Enterobacter sp. (strain 638) GN=sfcA PE=3 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 7.608e-28
Identity = 57/143 (39.86%), Postives = 98/143 (68.53%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER +  L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSAEERSSFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRSRGVFISYENRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 7.608e-28
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
The following BLAST results are available for this feature:
BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_PECCP3.209e-2942.66NAD-dependent malic enzyme OS=Pectobacterium carot... [more]
MAO1_AERS43.209e-2939.75NAD-dependent malic enzyme OS=Aeromonas salmonicid... [more]
MAO1_SERP55.440e-2941.96NAD-dependent malic enzyme OS=Serratia proteamacul... [more]
MAO1_AERHH9.220e-2939.13NAD-dependent malic enzyme OS=Aeromonas hydrophila... [more]
MAO12_PHOPR9.221e-2937.76NAD-dependent malic enzyme 2 OS=Photobacterium pro... [more]
MAO1_VIBFM2.649e-2840.56NAD-dependent malic enzyme OS=Vibrio fischeri (str... [more]
MAO1_VIBF12.649e-2840.56NAD-dependent malic enzyme OS=Vibrio fischeri (str... [more]
MAO1_ALISL2.649e-2840.56NAD-dependent malic enzyme OS=Aliivibrio salmonici... [more]
MAO1_PHOLL5.844e-2843.36NAD-dependent malic enzyme OS=Photorhabdus lumines... [more]
MAO1_ENT387.608e-2839.86NAD-dependent malic enzyme OS=Enterobacter sp. (st... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC
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