CK936872 | Citrus Genome Database

Overview


Name	CK936872
Unique Name	CK936872
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	735

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SALAR (NAD-dependent malic enzyme OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.185e-27
Identity = 58/143 (40.56%), Postives = 96/143 (67.13%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 2.185e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SALA4 (NAD-dependent malic enzyme OS=Salmonella agona (strain SL483) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.185e-27
Identity = 58/143 (40.56%), Postives = 96/143 (67.13%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 2.185e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ERWT9 (NAD-dependent malic enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.185e-27
Identity = 59/143 (41.26%), Postives = 96/143 (67.13%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER+   L GLLP  V S + Q ++     + ++    K+V +  +++ NE LFY+LL +++EE++P++YTPTVG AC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFSIEERNDFNLNGLLPETVESIEEQAERAWRQFQDFKNNNDKHVYLRNIQDTNETLFYRLLDNHLEEMMPIIYTPTVGAACEHFSEIYRRARGLFISYPNRANIEDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 2.185e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_EDWI9 (NAD-dependent malic enzyme OS=Edwardsiella ictaluri (strain 93-146) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.185e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+E+ER+   L GLLP A+ + + Q ++       +    ++++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+I+   + +I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFSEEERNTFNLNGLLPEAIETIEEQVERAYRQFCDFHSATEQHIYLRNIQDTNETLFYRLLRSHLSEMMPIIYTPTVGEACEHFSYIYRRARGLFIAYPNRDRIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 2.185e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_COLP3 (NAD-dependent malic enzyme OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 2.185e-27
Identity = 56/143 (39.16%), Postives = 95/143 (66.43%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+++ER +  L GLLPP   S   Q ++       ++  + K++ +  + + NE LF+KL+ +N+ E++P++YTPTVG+AC+++  I+R  +GL+IS +++  I ++L+N  +  ++VIVVTDGERI
Sbjct:   13 AGPSLLETPLLNKGSAFSKEERGSFNLTGLLPPRFESIDEQAERAFRQYSCFQTNINKHIYLRAIHDNNETLFFKLVQNNLAEMMPIIYTPTVGDACEQFSDIYRSSRGLFISYEDRFNIDDMLRNATKNKVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 2.185e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_BDEBA (NAD-dependent malic enzyme OS=Bdellovibrio bacteriovorus GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 2.844e-27
Identity = 58/143 (40.56%), Postives = 95/143 (66.43%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            SG  LL     NKG AF+E+ER    L GL+P  + + + Q +++ +  R  +  + K+V +  L++ NE LFY L+++++ E++P++YTP VGEAC+ +  I+R  +GL+IS   K +I E+L+N  ++ ++VIVVTDGERI
Sbjct:   16 SGTVLLEMSLLNKGSAFSEEERTHLNLHGLVPQTIETIEEQAERVYSQYRLLKADIDKHVYLRNLQDTNETLFYHLVVNHLTEMMPIIYTPVVGEACEHFSEIYRASRGLFISYPNKDRIDEMLRNVTKQKVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 2.844e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHESW (NAD-dependent malic enzyme OS=Shewanella sp. (strain W3-18-1) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.844e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.844e-27
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEPC (NAD-dependent malic enzyme OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.844e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.844e-27
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB9 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS195) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.844e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.844e-27
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB8 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS185) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.844e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.844e-27
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184

The following BLAST results are available for this feature:

BLAST of CK936872 vs. ExPASy Swiss-Prot

BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
MAO1_SALAR	2.185e-27	40.56	NAD-dependent malic enzyme OS=Salmonella arizonae ...	[more]
MAO1_SALA4	2.185e-27	40.56	NAD-dependent malic enzyme OS=Salmonella agona (st...	[more]
MAO1_ERWT9	2.185e-27	41.26	NAD-dependent malic enzyme OS=Erwinia tasmaniensis...	[more]
MAO1_EDWI9	2.185e-27	39.16	NAD-dependent malic enzyme OS=Edwardsiella ictalur...	[more]
MAO1_COLP3	2.185e-27	39.16	NAD-dependent malic enzyme OS=Colwellia psychreryt...	[more]
MAO1_BDEBA	2.844e-27	40.56	NAD-dependent malic enzyme OS=Bdellovibrio bacteri...	[more]
MAO1_SHESW	2.844e-27	39.16	NAD-dependent malic enzyme OS=Shewanella sp. (stra...	[more]
MAO1_SHEPC	2.844e-27	39.16	NAD-dependent malic enzyme OS=Shewanella putrefaci...	[more]
MAO1_SHEB9	2.844e-27	39.16	NAD-dependent malic enzyme OS=Shewanella baltica (...	[more]
MAO1_SHEB8	2.844e-27	39.16	NAD-dependent malic enzyme OS=Shewanella baltica (...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC