CK936872

Overview
NameCK936872
Unique NameCK936872
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CK936872_ssr16 microsatellite CK936872_ssr16:16..27. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig3937 contig Csv1_Contig3937:130..862. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB5 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.844e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.844e-27
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHEB2 (NAD-dependent malic enzyme OS=Shewanella baltica (strain OS223) GN=sfcA PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 2.844e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  +L  P  NKG AF+E+ER    L GL+P A+ + + Q  +  +  R +   L K++ +  +++ NE LFY+L+ +N+ E++P++YTPTVG AC+++   +RR +GL+IS   K +I ++L N   + +++IVVTDGERI
Sbjct:   13 AGPAILEAPLINKGSAFSEEERIFFNLEGLVPYAIETIEEQASRAYDQFRSFNNDLDKHIYLRNIQDTNETLFYRLVQNNISEMMPIIYTPTVGLACERFSKNYRRNRGLFISYPNKDRIDDILNNSTRQKVKIIVVTDGERI 155

HSP 2 Score: 20.7866 bits (42), Expect = 2.844e-27
Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT+ GGI P
Sbjct:  170 IGKLSLYTSCGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBHB (NAD-dependent malic enzyme OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 2.844e-27
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER +  L GLLP    + Q Q ++     + +E  + K++ +  +++ NE L+Y+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL+IS   + +I ++L N    +++VIVVTDGERI
Sbjct:   13 AGPALLATPLLNKGSAFSGEERSSFNLEGLLPETTETIQEQVERAYQQYKSFESDMDKHIYLRNIQDTNETLYYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYPNRDRIDDLLNNAANHNVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 2.844e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPY (NAD-dependent malic enzyme OS=Yersinia pseudotuberculosis serotype O:3 (strain YPIII) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPS (NAD-dependent malic enzyme OS=Yersinia pseudotuberculosis GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPP (NAD-dependent malic enzyme OS=Yersinia pestis (strain Pestoides F) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPN (NAD-dependent malic enzyme OS=Yersinia pestis bv. Antiqua (strain Nepal516) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPE (NAD-dependent malic enzyme OS=Yersinia pestis GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPB (NAD-dependent malic enzyme OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERPA (NAD-dependent malic enzyme OS=Yersinia pestis bv. Antiqua (strain Antiqua) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
The following BLAST results are available for this feature:
BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_SHEB52.844e-2739.16NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_SHEB22.844e-2739.16NAD-dependent malic enzyme OS=Shewanella baltica (... [more]
MAO1_VIBHB2.844e-2738.46NAD-dependent malic enzyme OS=Vibrio harveyi (stra... [more]
MAO1_YERPY3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pseudotuber... [more]
MAO1_YERPS3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pseudotuber... [more]
MAO1_YERPP3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pestis (str... [more]
MAO1_YERPN3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pestis bv. ... [more]
MAO1_YERPE3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pestis GN=s... [more]
MAO1_YERPB3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pseudotuber... [more]
MAO1_YERPA3.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pestis bv. ... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC
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