CK936872

Overview
NameCK936872
Unique NameCK936872
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length735
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERP3 (NAD-dependent malic enzyme OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.702e-27
Identity = 59/143 (41.26%), Postives = 95/143 (66.43%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER+   L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERNHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 3.702e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_YERE8 (NAD-dependent malic enzyme OS=Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 4.819e-27
Identity = 59/143 (41.26%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AFT  ER    L GLLP AV + + Q ++     + ++    K++ +  +++ NE LFY+LL  ++ E++P++YTPTVGEAC+ +  I+RR +GL+IS   +  I ++L+N  +++++VIVVTDGERI
Sbjct:   16 AGPILLEFPLLNKGSAFTNDERSHFNLHGLLPEAVETIEEQAERAYRQYQDFKNDDDKHIYLRNIQDTNETLFYRLLEAHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNREHIDDMLQNATKQNVKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 4.819e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_CITK8 (NAD-dependent malic enzyme OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 4.819e-27
Identity = 57/143 (39.86%), Postives = 97/143 (67.83%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V S + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSIEERRNFNLLGLLPEVVESIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLDEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 4.819e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_VIBVU (NAD-dependent malic enzyme OS=Vibrio vulnificus GN=sfcA PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 4.819e-27
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER +  L GLLP    + Q Q ++     + +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL++S   + +I ++L N    +++VIVVTDGERI
Sbjct:   13 AGPALLSTPLLNKGSAFSAEERISFNLEGLLPETTETIQEQVERAYMQYKAFESDMDKHIYLRNIQDTNETLFYRLVQNHITEMMPIIYTPTVGAACENFSNIYRRGRGLFVSYANRDRIDDILNNASNHNVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 4.819e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO11_VIBVY (NAD-dependent malic enzyme 1 OS=Vibrio vulnificus (strain YJ016) GN=sfcA1 PE=3 SV=2)

HSP 1 Score: 118.627 bits (296), Expect = 4.819e-27
Identity = 55/143 (38.46%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER +  L GLLP    + Q Q ++     + +E  + K++ +  +++ NE LFY+L+ +++ E++P++YTPTVG AC+ + +I+RR +GL++S   + +I ++L N    +++VIVVTDGERI
Sbjct:   13 AGPALLSTPLLNKGSAFSAEERISFNLEGLLPETTETIQEQVERAYMQYKAFESDMDKHIYLRNIQDTNETLFYRLVQNHITEMMPIIYTPTVGAACENFSNIYRRGRGLFVSYANRDRIDDILNNASNHNVKVIVVTDGERI 155

HSP 2 Score: 21.9422 bits (45), Expect = 4.819e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  170 IGKLSLYTACGGISP 184
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_KLEP7 (NAD-dependent malic enzyme OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 6.273e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +++VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERSNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLIGNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNLDDILQNVPNHNVKVIVVTDGERI 158

HSP 2 Score: 20.4014 bits (41), Expect = 6.273e-27
Identity = 8/15 (53.33%), Postives = 9/15 (60.00%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT  GGI P
Sbjct:  173 IGKLSLYTTCGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ENTS8 (NAD-dependent malic enzyme OS=Enterobacter sakazakii (strain ATCC BAA-894) GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 94/143 (65.73%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++       ++  + K++ +  +++ NE LFY+LL +++E ++P++YTPTVG AC+++  I+RR +G++IS   +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERSNFNLLGLLPEVVETIEEQAERAWRQFEDFKTDIDKHIYLRNIQDTNETLFYRLLENHLEVMMPIIYTPTVGSACERFSEIYRRARGVFISWPNRHNMDDILQNVPIHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_KLEP3 (NAD-dependent malic enzyme OS=Klebsiella pneumoniae (strain 342) GN=sfcA PE=3 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ +++EE++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +++VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSLEERSNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLIGNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYQNRHNLDDILQNVPNHNVKVIVVTDGERI 158

HSP 2 Score: 20.4014 bits (41), Expect = 8.165e-27
Identity = 8/15 (53.33%), Postives = 9/15 (60.00%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYT  GGI P
Sbjct:  173 IGKLSLYTTCGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHISS (NAD-dependent malic enzyme OS=Shigella sonnei (strain Ss046) GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
[Close]
BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHIFL (NAD-dependent malic enzyme OS=Shigella flexneri GN=sfcA PE=3 SV=4)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2 
Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1 
Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187
The following BLAST results are available for this feature:
BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_YERP33.702e-2741.26NAD-dependent malic enzyme OS=Yersinia pseudotuber... [more]
MAO1_YERE84.819e-2741.26NAD-dependent malic enzyme OS=Yersinia enterocolit... [more]
MAO1_CITK84.819e-2739.86NAD-dependent malic enzyme OS=Citrobacter koseri (... [more]
MAO1_VIBVU4.819e-2738.46NAD-dependent malic enzyme OS=Vibrio vulnificus GN... [more]
MAO11_VIBVY4.819e-2738.46NAD-dependent malic enzyme 1 OS=Vibrio vulnificus ... [more]
MAO1_KLEP76.273e-2739.16NAD-dependent malic enzyme OS=Klebsiella pneumonia... [more]
MAO1_ENTS88.165e-2739.16NAD-dependent malic enzyme OS=Enterobacter sakazak... [more]
MAO1_KLEP38.165e-2739.16NAD-dependent malic enzyme OS=Klebsiella pneumonia... [more]
MAO1_SHISS8.165e-2739.16NAD-dependent malic enzyme OS=Shigella sonnei (str... [more]
MAO1_SHIFL8.165e-2739.16NAD-dependent malic enzyme OS=Shigella flexneri GN... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC
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