CK936872 | Citrus Genome Database

Overview


Name	CK936872
Unique Name	CK936872
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	735

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHIF8 (NAD-dependent malic enzyme OS=Shigella flexneri serotype 5b (strain 8401) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHIBS (NAD-dependent malic enzyme OS=Shigella boydii serotype 4 (strain Sb227) GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_SHIB3 (NAD-dependent malic enzyme OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ESCF3 (NAD-dependent malic enzyme OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOUT (NAD-dependent malic enzyme OS=Escherichia coli (strain UTI89 / UPEC) GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOSM (NAD-dependent malic enzyme OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLU (NAD-dependent malic enzyme OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLI (NAD-dependent malic enzyme OS=Escherichia coli (strain K12) GN=sfcA PE=3 SV=4)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLC (NAD-dependent malic enzyme OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

BLAST of CK936872 vs. ExPASy Swiss-Prot
Match: MAO1_ECOL6 (NAD-dependent malic enzyme OS=Escherichia coli O6 GN=sfcA PE=3 SV=2)

HSP 1 Score: 117.857 bits (294), Expect = 8.165e-27
Identity = 56/143 (39.16%), Postives = 97/143 (67.83%), Query Frame = 2

Query:  218 SGYCLLRDPRHNKGLAFTEKERDAHYLRGLLPPAVISQQLQEKKLMNSIRQYEVPLQKYVAMMELEERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQGLYISLKEKGKILEVLKNWPERSIQVIVVTDGERI 646
            +G  LL  P  NKG AF+ +ER    L GLLP  V + + Q ++     + ++  + K++ +  +++ NE LFY+L+ ++++E++PV+YTPTVG AC+++  I+RR +G++IS + +  + ++L+N P  +I+VIVVTDGERI
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERI 158

HSP 2 Score: 21.9422 bits (45), Expect = 8.165e-27
Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = 1

Query:  691 LGNWLLYTALGGIRP 735
            +G   LYTA GGI P
Sbjct:  173 IGKLSLYTACGGISP 187

The following BLAST results are available for this feature:

BLAST of CK936872 vs. ExPASy Swiss-Prot

BLAST of CK936872 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140


Match Name	E-value	Identity	Description
MAO1_SHIF8	8.165e-27	39.16	NAD-dependent malic enzyme OS=Shigella flexneri se...	[more]
MAO1_SHIBS	8.165e-27	39.16	NAD-dependent malic enzyme OS=Shigella boydii sero...	[more]
MAO1_SHIB3	8.165e-27	39.16	NAD-dependent malic enzyme OS=Shigella boydii sero...	[more]
MAO1_ESCF3	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia ferguson...	[more]
MAO1_ECOUT	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOSM	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOLU	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O17...	[more]
MAO1_ECOLI	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOLC	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli (st...	[more]
MAO1_ECOL6	8.165e-27	39.16	NAD-dependent malic enzyme OS=Escherichia coli O6 ...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004517_A07 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0002_IVF_A07 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936872 ID=CK936872; Name=CK936872; organism=Citrus sinensis; type=EST; length=735bp
GGGGAAGGGAGTGAGTGTTTGTTTGTTTTCGTTTTTGTGAAAAGCAGAAA
AAGTAAAAAGAGTGAGAATGGAGAGCACCATGAAGGAGATGAGAGACGGA
GCTTCGGTTCTCGACTTCGACTCGAAATCCACCGTCGAAGGCGGCGTTGA
AGACGTCTACGGTGAGGATCGTGCCACCGAGGACCAGCTCGTCACTCCCT
GGACCATCTCTGTTGCCAGCGGGTACTGTTTGTTGAGGGATCCACGCCAT
AACAAAGGGCTTGCTTTCACTGAGAAAGAGAGGGATGCCCACTACTTGCG
CGGCCTTTTGCCTCCAGCTGTTATAAGTCAACAACTTCAGGAGAAGAAAT
TGATGAACAGCATCAGACAATATGAAGTTCCGCTGCAAAAATATGTGGCC
ATGATGGAACTTGAGGAGAGAAATGAAAGACTGTTCTATAAGCTACTGAT
TGATAATGTTGAGGAACTTCTCCCAGTCGTTTACACTCCAACTGTTGGTG
AAGCTTGCCAGAAATATGGAAGTATCTTCAGGCGTCCTCAGGGTCTATAC
ATAAGTCTGAAAGAGAAGGGAAAGATTCTTGAGGTTTTGAAGAACTGGCC
TGAAAGGAGTATTCAGGTTATTGTTGTCACAGATGGTGAGCGCATTTTGG
GACTTGGGGATCTTGGCTNTCAGGGGATGGGAATTTCCTGTTGGGAAATT
GGCTTTTGTACACAGCACTTGGAGGCATTCGTCCC