CK936768

Overview
NameCK936768
Unique NameCK936768
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length723
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_WHEAT (Adenosylhomocysteinase OS=Triticum aestivum GN=SAHH PE=2 SV=1)

HSP 1 Score: 332.413 bits (851), Expect = 1.484e-90
Identity = 159/198 (80.30%), Postives = 173/198 (87.37%), Query Frame = 1
Query:  130 MALSVEKSASGREFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDHXXXXXXXXXXXXXXWKGETLQEYWWCTEKALDWGPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKMKER 723
            MALSVEK++SGRE+KVKDL QADFGRLE+ELAEVEMPGLMACR EFGPSQPFKGA+I+GSLHMTIQTAVLIETLTALGAEVRWCSCNIFS+QDH              WKGETL+EYWWCTE+ LDWG GGGPDLIVDDGGDATLLIHEGVKAEE +EK+GK+PDP STDN EF+IVLTIIRDGL+ D  KY KMKER
Sbjct:    1 MALSVEKTSSGREYKVKDLFQADFGRLELELAEVEMPGLMACRTEFGPSQPFKGARISGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSSQDHAAAAIARDSAAVFAWKGETLEEYWWCTERCLDWGVGGGPDLIVDDGGDATLLIHEGVKAEEEFEKSGKVPDPESTDNPEFKIVLTIIRDGLKTDASKYRKMKER 198          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_SOLLC (Adenosylhomocysteinase OS=Solanum lycopersicum GN=SAHH PE=2 SV=1)

HSP 1 Score: 323.939 bits (829), Expect = 5.277e-88
Identity = 157/198 (79.29%), Postives = 168/198 (84.85%), Query Frame = 1
Query:  130 MALSVEKSASGREFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDHXXXXXXXXXXXXXXWKGETLQEYWWCTEKALDWGPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKMKER 723
            MAL VEK+ SGRE+KVKD+SQADFGRLEIELAEVEMPGLMA RAEFGPSQP KGAKIT SLHMTIQTA LIETLTALGAEVRWCSCNIFSTQDH              WKGETLQEYWWCTE+ALDWGPGGGPDLIVDDGGDATLLIHEGVKAEE + K G +PDP STDN EFQ+VLTII++ L+ DP +Y KMKER
Sbjct:    1 MALLVEKTTSGREYKVKDMSQADFGRLEIELAEVEMPGLMASRAEFGPSQPVKGAKITCSLHMTIQTAFLIETLTALGAEVRWCSCNIFSTQDHAAAAIARDSAAVFAWKGETLQEYWWCTERALDWGPGGGPDLIVDDGGDATLLIHEGVKAEEEFAKNGTVPDPTSTDNVEFQLVLTIIKESLKTDPLRYTKMKER 198          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_STRGG (Adenosylhomocysteinase OS=Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) GN=ahcY PE=3 SV=1)

HSP 1 Score: 213.772 bits (543), Expect = 7.690e-55
Identity = 109/195 (55.90%), Postives = 135/195 (69.23%), Query Frame = 1
Query:  163 REFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH---------XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDW--GPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKM 714
            ++FKV DLS A FGR EI LAE EMPGLM+ R EF  +QP  GA+ITGSLHMT+QTAVLIETL ALGAEVRW SCNIFSTQDH                       WKGETL+EYWWCTE+AL W   P GGP++I+DDGGDATLL+H+GV+    +EK G  PDP++ D+ E+  +LT++   L   P+K+ ++
Sbjct:    8 QDFKVADLSLAPFGRKEITLAEHEMPGLMSIRKEFAAAQPLAGARITGSLHMTVQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAVGPNGTPEAPAGVPVFAWKGETLEEYWWCTEQALTWPNTPTGGPNMILDDGGDATLLVHKGVE----FEKAGAAPDPSTADSEEYAHILTLLNRTLGEAPQKWTQL 198          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_PLAYO (Adenosylhomocysteinase OS=Plasmodium yoelii yoelii GN=PY02893 PE=3 SV=1)

HSP 1 Score: 209.149 bits (531), Expect = 1.894e-53
Identity = 100/186 (53.76%), Postives = 131/186 (70.43%), Query Frame = 1
Query:  172 KVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH-XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDWGPGG-GPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKMKER 723
            K+KDLS A FG+L++E++E EMPG+M  R E+   +PFKGAKITG LHMTI+TA+LIETL  LGA +RWCSCNIFST D+               W+GETL+EYWWC EKAL WG  G GPDLIVDDG DA+ L+H+G + E++YE+   LPDP S  N E +  L++I+  +  +PKK+  M ++
Sbjct:    7 KIKDLSLAPFGKLQMEISETEMPGIMTIREEYEKLKPFKGAKITGCLHMTIETALLIETLQKLGARIRWCSCNIFSTLDYAAAAVSTLENVSVFAWRGETLEEYWWCVEKALTWGENGEGPDLIVDDGADASYLVHKGAEYEKLYEEKKILPDPESGKNEEERCFLSLIKSSILKNPKKWTNMAKK 192          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_PLAF7 (Adenosylhomocysteinase OS=Plasmodium falciparum (isolate 3D7) GN=PFE1050w PE=1 SV=2)

HSP 1 Score: 207.994 bits (528), Expect = 4.220e-53
Identity = 97/187 (51.87%), Postives = 131/187 (70.05%), Query Frame = 1
Query:  172 KVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH-XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDWGPG--GGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKMKER 723
            KVKD+S A FG++++E++E EMPGLM  R E+G  QP K AKITG LHMT++ A+LIETL  LGA++RWCSCNI+ST D+               WK ETL+EYWWC E AL WG G   GPD+IVDDGGDATLL+H+GV+ E++YE+   LPDP    N E +  LT++++ +  +PKK+  + ++
Sbjct:    7 KVKDISLAPFGKMQMEISENEMPGLMRIREEYGKDQPLKNAKITGCLHMTVECALLIETLQKLGAQIRWCSCNIYSTADYAAAAVSTLENVTVFAWKNETLEEYWWCVESALTWGDGDDNGPDMIVDDGGDATLLVHKGVEYEKLYEEKNILPDPEKAKNEEERCFLTLLKNSILKNPKKWTNIAKK 193          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_STRCO (Adenosylhomocysteinase OS=Streptomyces coelicolor GN=ahcY PE=3 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 9.401e-53
Identity = 105/195 (53.85%), Postives = 134/195 (68.72%), Query Frame = 1
Query:  163 REFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH---------XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDW--GPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKM 714
            ++FKV DLS A FGR EI LAE EMPGLMA R E+  +QP  GA++TGSLHMT+QTAVLIETL ALGAEVRW SCNIFSTQDH                       WKGETL+EYWWCTE+AL W   P GGP++I+DDGGDATLL+H+GV+    YEK GK+P   + ++ E +++L ++   +   P+K+ ++
Sbjct:    8 QDFKVADLSLAAFGRKEITLAEHEMPGLMAIRKEYAEAQPLAGARVTGSLHMTVQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAVGPNGTPDNPQGVPVFAWKGETLEEYWWCTEQALTWPNTPTGGPNMILDDGGDATLLVHKGVE----YEKDGKVPSVDTAESDEHRVILELLTRTVGESPQKWTQL 198          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_DESDG (Adenosylhomocysteinase OS=Desulfovibrio desulfuricans (strain G20) GN=ahcY PE=3 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.094e-52
Identity = 108/187 (57.75%), Postives = 130/187 (69.52%), Query Frame = 1
Query:  166 EFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH-XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDWGPGGGPDLIVDDGGDATLLIHEGVKAEE---IYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKM 714
            E KVKDLSQADFG  E++L+E EMPGLM   A+ G  +P KG K+TGSLHMTIQTA+LI+TL  LGA++RW SCNIFSTQDH               WKGETL+EYWWCTE AL W  G GPDLIVDDGGDAT+LIH+GV+AE+   + EK        S DN EFQIV+  I   ++ DP K+ ++
Sbjct:   15 ENKVKDLSQADFGYKEMQLSEREMPGLMELIAKHGAEKPLKGLKVTGSLHMTIQTAMLIKTLYELGADIRWASCNIFSTQDHAAAAIADSGMAKVFAWKGETLEEYWWCTEMALTWPDGSGPDLIVDDGGDATMLIHKGVEAEKNPALLEK--------SYDNKEFQIVMNRIALSMKNDPGKWTRV 193          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_DESAD (Adenosylhomocysteinase OS=Desulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763) GN=ahcY PE=3 SV=1)

HSP 1 Score: 205.682 bits (522), Expect = 2.094e-52
Identity = 107/193 (55.44%), Postives = 131/193 (67.88%), Query Frame = 1
Query:  148 KSASGREFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH-XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDWGPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKMKER 723
            K  S  ++KV D+S AD+G  E++L+E EMPGLM+ R ++G  +P KG K+ GSLHMTIQTA+LIETL ALGA++RW SCNIFSTQDH               WKGETL+EYWWCTE+AL W  G GPDLIVDDGGDATLLIH GVKA    EK   + D   TDN EFQ VL  ++  +   P K+  + E+
Sbjct:    3 KVDSKLDYKVADISLADWGNKEMQLSEREMPGLMSIREKYGKEKPLKGLKVMGSLHMTIQTAMLIETLHALGADIRWASCNIFSTQDHAAAAIAANGTAKVFAWKGETLEEYWWCTEQALTWPDGSGPDLIVDDGGDATLLIHHGVKA----EKDASILD-EKTDNKEFQCVLDRLKLSVAETPGKWTAIAEK 190          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_STRAW (Adenosylhomocysteinase OS=Streptomyces avermitilis GN=ahcY PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 4.666e-52
Identity = 103/195 (52.82%), Postives = 134/195 (68.72%), Query Frame = 1
Query:  163 REFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH---------XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDW--GPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKM 714
            ++FKV DLS A+FGR EI LAE EMPGLM+ R E+  +QP  GA++TGSLHMT+QTAVLIETL ALGAEVRW SCNIFSTQDH                       WKGETL+EYWWCTE+AL W   P GGP++I+DDGGDATLL+H+GV+    YEK GK+P   + ++ E +++L ++   +    +K+ ++
Sbjct:    8 QDFKVADLSLAEFGRKEITLAEHEMPGLMSIRKEYAEAQPLAGARVTGSLHMTVQTAVLIETLVALGAEVRWASCNIFSTQDHAAAAIAVGPNGTPDNPQGVPVFAWKGETLEEYWWCTEQALTWPNTPTGGPNMILDDGGDATLLVHKGVE----YEKDGKVPSVDTAESDEHRVILELLHRTITDGSQKWTQL 198          
BLAST of CK936768 vs. ExPASy Swiss-Prot
Match: SAHH_STRAA (Adenosylhomocysteinase OS=Streptomyces argillaceus GN=ahcY PE=3 SV=1)

HSP 1 Score: 204.527 bits (519), Expect = 4.666e-52
Identity = 104/199 (52.26%), Postives = 134/199 (67.34%), Query Frame = 1
Query:  151 SASGREFKVKDLSQADFGRLEIELAEVEMPGLMACRAEFGPSQPFKGAKITGSLHMTIQTAVLIETLTALGAEVRWCSCNIFSTQDH---------XXXXXXXXXXXXXXWKGETLQEYWWCTEKALDW--GPGGGPDLIVDDGGDATLLIHEGVKAEEIYEKTGKLPDPASTDNAEFQIVLTIIRDGLRADPKKYHKM 714
            SA   +FKV DLS A+FGR EI LAE EMPGLM+ R E+  +QP  GA++TGSLHMT+QTAVLIETL ALGA+VRW SCNIFSTQ H                       WKGETL+EYWWCTE+AL W   P GGP++I+DDGGDATLL+H+GV+    YEK GK+P   + +N E +++L ++   +    +K+ ++
Sbjct:    2 SADFTDFKVADLSLAEFGRKEITLAEHEMPGLMSIRREYAEAQPLAGARVTGSLHMTVQTAVLIETLVALGAQVRWASCNIFSTQGHAAAAIAVGPDGTPDNPRAVPVFAWKGETLEEYWWCTEQALTWPDSPTGGPNMILDDGGDATLLVHKGVE----YEKDGKVPSVDTAENDEHRVILELLNRTITDGSQKWTQL 196          
The following BLAST results are available for this feature:
BLAST of CK936768 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 235
Match NameE-valueIdentityDescription
SAHH_WHEAT1.484e-9080.30Adenosylhomocysteinase OS=Triticum aestivum GN=SAH... [more]
SAHH_SOLLC5.277e-8879.29Adenosylhomocysteinase OS=Solanum lycopersicum GN=... [more]
SAHH_STRGG7.690e-5555.90Adenosylhomocysteinase OS=Streptomyces griseus sub... [more]
SAHH_PLAYO1.894e-5353.76Adenosylhomocysteinase OS=Plasmodium yoelii yoelii... [more]
SAHH_PLAF74.220e-5351.87Adenosylhomocysteinase OS=Plasmodium falciparum (i... [more]
SAHH_STRCO9.401e-5353.85Adenosylhomocysteinase OS=Streptomyces coelicolor ... [more]
SAHH_DESDG2.094e-5257.75Adenosylhomocysteinase OS=Desulfovibrio desulfuric... [more]
SAHH_DESAD2.094e-5255.44Adenosylhomocysteinase OS=Desulfovibrio salexigens... [more]
SAHH_STRAW4.666e-5252.82Adenosylhomocysteinase OS=Streptomyces avermitilis... [more]
SAHH_STRAA4.666e-5252.26Adenosylhomocysteinase OS=Streptomyces argillaceus... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004518_B05 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IF_B05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936768 ID=CK936768; Name=CK936768; organism=Citrus sinensis; type=EST; length=723bp
GGGGGCTCACACCCACTCTATCTCTCTCGCTTGTACAATCATTCGCTTTG
TTTGGTTGGTTTCCAAAGAGAGAGTAGAGTGCCCTCTCTTCCTTCTTCCT
TTTAGATCTATCAATTCTATTAATAAGCCATGGCTTTGTCCGTCGAGAAA
TCCGCTAGCGGTCGTGAATTCAAGGTGAAGGATCTGTCCCAGGCTGACTT
CGGCCGCCTCGAAATCGAGTTGGCCGAAGTCGAAATGCCGGGGCTCATGG
CTTGCCGCGCCGAGTTCGGCCCTTCACAGCCCTTCAAAGGAGCCAAGATC
ACCGGTTCACTCCACATGACCATCCAGACCGCCGTCCTCATCGAGACCCT
CACCGCTCTCGGCGCCGAAGTCCGCTGGTGCTCCTGCAACATCTTCTCAA
CCCAAGACCACGCGGCCGCCGCCATCGCACGTGACTCCGCCTCCGTCTTT
GCCTGGAAGGGTGAGACCCTCCAGGAGTACTGGTGGTGCACGGAGAAAGC
CCTGGACTGGGGTCCCGGCGGCGGCCCAGATCTCATCGTCGATGACGGTG
GTGACGCCACGTTGTTGATCCACGAGGGAGTCAAAGCTGAGGAGATCTAC
GAGAAGACCGGGAAGTTGCCCGACCCGGCCTCCACCGACAACGCCGAGTT
TCAGATTGTTTTGACTATCATTAGAGATGGGTTGAGGGCCGATCCCAAGA
AGTACCACAAAATGAAGGAAAGA
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