CK936525

Overview
NameCK936525
Unique NameCK936525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length840
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SODGM (NAD-dependent malic enzyme OS=Sodalis glossinidius (strain morsitans) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 9.605e-26
Identity = 57/138 (41.30%), Postives = 96/138 (69.57%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER++  L+GLLP TV + E Q ++     + ++  + +++ + ++Q+ N+ LFY+LL  ++ E+LPI+YTPTVG+AC+++  IY R +GVFIS  +  K+ ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFSLEERDNFNLQGLLPDTVETIEEQAERAWRQYQDFRTNIDRHIYLRNIQDTNETLFYRLLAAHLAEMLPIIYTPTVGDACERFSDIYRRARGVFISYNNCDKIEDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PSYCK (NAD-dependent malic enzyme OS=Psychrobacter cryohalolentis (strain K5) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 9.605e-26
Identity = 55/138 (39.86%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS +ER+S  L GLLP  + + E Q  +    +R +   + K++ + ++Q+ N+ LF+ L+  ++EE++P++YTPTVG+AC+K+  IY R +G+FIS  ++ K+ ++L+N  ++N++VIVVT
Sbjct:   12 AGPALLETPLLNKGSAFSSEERDSFNLTGLLPHNIETIEEQSSRAYLQLRSFTSDMDKHIYLRNIQDTNETLFHHLIEQHIEEVMPLIYTPTVGQACEKFSQIYRRKRGLFISYPERHKIDDMLQNATKQNVKVIVVT 149
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ENT38 (NAD-dependent malic enzyme OS=Enterobacter sp. (strain 638) GN=sfcA PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.254e-25
Identity = 57/138 (41.30%), Postives = 95/138 (68.84%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER+S  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSAEERSSFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRSRGVFISYENRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ERWT9 (NAD-dependent malic enzyme OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=sfcA PE=3 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.638e-25
Identity = 58/138 (42.03%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ERN   L GLLP TV S E Q ++     + ++    K++ + ++Q+ N+ LFY+LL +++EE++PI+YTPTVG AC+ +  IY R +G+FIS  ++  + ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFSIEERNDFNLNGLLPETVESIEEQAERAWRQFQDFKNNNDKHVYLRNIQDTNETLFYRLLDNHLEEMMPIIYTPTVGAACEHFSEIYRRARGLFISYPNRANIEDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SERP5 (NAD-dependent malic enzyme OS=Serratia proteamaculans (strain 568) GN=sfcA PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 2.140e-25
Identity = 56/138 (40.58%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AF+E+ERN   L+GLLP  V + E Q ++     + ++    K++ + ++Q+ N+ LFY+LL  ++ E++PI+YTPTVGEAC+ +  IY R +G+FIS  ++ ++ ++L+N  ++N++VIVVT
Sbjct:   16 AGPILLEFPLLNKGSAFTEEERNHFNLQGLLPDAVETIEEQAERAYRQYQDFKNDSDKHIYLRNIQDTNETLFYRLLDSHLSEMMPIIYTPTVGEACEHFSDIYRRARGLFISYPNRDRIDDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SALG2 (NAD-dependent malic enzyme OS=Salmonella gallinarum (strain 287/91 / NCTC 13346) GN=sfcA PE=3 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 2.140e-25
Identity = 57/138 (41.30%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V S E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS  ++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSAEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SALTY (NAD-dependent malic enzyme OS=Salmonella typhimurium GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.795e-25
Identity = 57/138 (41.30%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V S E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS  ++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SALTI (NAD-dependent malic enzyme OS=Salmonella typhi GN=sfcA PE=3 SV=2)

HSP 1 Score: 115.931 bits (289), Expect = 2.795e-25
Identity = 57/138 (41.30%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V S E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS  ++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SALSV (NAD-dependent malic enzyme OS=Salmonella schwarzengrund (strain CVM19633) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.795e-25
Identity = 57/138 (41.30%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V S E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS  ++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SALPB (NAD-dependent malic enzyme OS=Salmonella paratyphi B (strain ATCC BAA-1250 / SPB7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 115.931 bits (289), Expect = 2.795e-25
Identity = 57/138 (41.30%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V S E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ +++EE++P++YTPTVG AC+++  IY R +GVFIS  ++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSVEERRNFNLSGLLPEVVESIEEQAERAWLQYQGFKTEIDKHIYLRNIQDTNETLFYRLVQNHLEEMMPVIYTPTVGAACERFSEIYRRARGVFISYPNRHNMDDILQNVPNHNIKVIVVT 153
The following BLAST results are available for this feature:
BLAST of CK936525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_SODGM9.605e-2641.30NAD-dependent malic enzyme OS=Sodalis glossinidius... [more]
MAO1_PSYCK9.605e-2639.86NAD-dependent malic enzyme OS=Psychrobacter cryoha... [more]
MAO1_ENT381.254e-2541.30NAD-dependent malic enzyme OS=Enterobacter sp. (st... [more]
MAO1_ERWT91.638e-2542.03NAD-dependent malic enzyme OS=Erwinia tasmaniensis... [more]
MAO1_SERP52.140e-2540.58NAD-dependent malic enzyme OS=Serratia proteamacul... [more]
MAO1_SALG22.140e-2541.30NAD-dependent malic enzyme OS=Salmonella gallinaru... [more]
MAO1_SALTY2.795e-2541.30NAD-dependent malic enzyme OS=Salmonella typhimuri... [more]
MAO1_SALTI2.795e-2541.30NAD-dependent malic enzyme OS=Salmonella typhi GN=... [more]
MAO1_SALSV2.795e-2541.30NAD-dependent malic enzyme OS=Salmonella schwarzen... [more]
MAO1_SALPB2.795e-2541.30NAD-dependent malic enzyme OS=Salmonella paratyphi... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004521_C09 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IVF_C09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936525 ID=CK936525; Name=CK936525; organism=Citrus sinensis; type=EST; length=840bp
GGGGAGACCCNNNAGACTCTCCCTTCTCTTCTCATTGCCTCTGCAACTTT
TTTTTCTATTTTTACTTCGTCACACATTTTTAATCAGCGCAAGACTCTCT
CTTCACACATTTCTCTCGCCATGTTTTCGTTAAACAGAAGCGCTTTTCTG
AATAATCCTGCGAGCATATCAGGCTCGTTCCCTGATAAGCATAGAAGAAT
ACTGCCTACAAGAGTGGTGGTTGCGGGCTCGTCTAACAGAGATAAGAGTA
ACGGTAGTCTTTTAATGGCAACCGATGAAAGCACAGCTACATTAAAAGAA
ATGAGAGATGGCTATGCCGAAGTTGTGGACCCCAAGTCCGGCGTCAGCGG
TGGGGTCCAGGACGTTTATGGTGAGGATACCGCCACCGAAGACCAGCCGG
TCACCCCTTGGTCTGTCTCAGTTGCTAGTGGGTATTCTTTGTTGAGAGAT
CCACACCACAACAAAGGGCTTGCCTTCTCTGAAAAAGAAAGGAATTCACA
TTACTTGCGTGGTCTTCTCCCCCCTACAGTTATATCCCAAGAGCTCCAGG
TGAAGAAAATGTTGCATAATATTCGGCAGTACCAAGTTCCACTCCAGAAG
TACATGGCCATGATGGATCTTCAGGAGAGAAATCAGAAACTGTTCTATAA
GCTTCTCATTGACAATGTTGAGGAGTTACTCCCAATTGTCTATACTCCAA
CTGTTGGAGAAGCTTGCCAGAAGTACGGGAGCATCTATAGTCGTCCTCAG
GGTGTTTTTATCAGTTTGAAGGATAAGGGCAAGGTTCTTGAGGTATTGAG
GAACTGGCCTGAGAAGAATATTCAAGTCATTGTTGTCACT
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