CK936525

Overview
NameCK936525
Unique NameCK936525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length840
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ENTS8 (NAD-dependent malic enzyme OS=Enterobacter sakazakii (strain ATCC BAA-894) GN=sfcA PE=3 SV=2)

HSP 1 Score: 114.39 bits (285), Expect = 8.131e-25
Identity = 57/138 (41.30%), Postives = 92/138 (66.67%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER++  L GLLP  V + E Q ++       ++  + K++ + ++Q+ N+ LFY+LL +++E ++PI+YTPTVG AC+++  IY R +GVFIS  ++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERSNFNLLGLLPEVVETIEEQAERAWRQFEDFKTDIDKHIYLRNIQDTNETLFYRLLENHLEVMMPIIYTPTVGSACERFSEIYRRARGVFISWPNRHNMDDILQNVPIHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SHISS (NAD-dependent malic enzyme OS=Shigella sonnei (strain Ss046) GN=sfcA PE=3 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SHIFL (NAD-dependent malic enzyme OS=Shigella flexneri GN=sfcA PE=3 SV=4)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SHIF8 (NAD-dependent malic enzyme OS=Shigella flexneri serotype 5b (strain 8401) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SHIBS (NAD-dependent malic enzyme OS=Shigella boydii serotype 4 (strain Sb227) GN=sfcA PE=3 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_SHIB3 (NAD-dependent malic enzyme OS=Shigella boydii serotype 18 (strain CDC 3083-94 / BS512) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ESCF3 (NAD-dependent malic enzyme OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECOUT (NAD-dependent malic enzyme OS=Escherichia coli (strain UTI89 / UPEC) GN=sfcA PE=3 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECOSM (NAD-dependent malic enzyme OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECOLU (NAD-dependent malic enzyme OS=Escherichia coli O17:K52:H18 (strain UMN026 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
The following BLAST results are available for this feature:
BLAST of CK936525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_ENTS88.131e-2541.30NAD-dependent malic enzyme OS=Enterobacter sakazak... [more]
MAO1_SHISS1.062e-2439.86NAD-dependent malic enzyme OS=Shigella sonnei (str... [more]
MAO1_SHIFL1.062e-2439.86NAD-dependent malic enzyme OS=Shigella flexneri GN... [more]
MAO1_SHIF81.062e-2439.86NAD-dependent malic enzyme OS=Shigella flexneri se... [more]
MAO1_SHIBS1.062e-2439.86NAD-dependent malic enzyme OS=Shigella boydii sero... [more]
MAO1_SHIB31.062e-2439.86NAD-dependent malic enzyme OS=Shigella boydii sero... [more]
MAO1_ESCF31.062e-2439.86NAD-dependent malic enzyme OS=Escherichia ferguson... [more]
MAO1_ECOUT1.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECOSM1.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECOLU1.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O17... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004521_C09 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IVF_C09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936525 ID=CK936525; Name=CK936525; organism=Citrus sinensis; type=EST; length=840bp
GGGGAGACCCNNNAGACTCTCCCTTCTCTTCTCATTGCCTCTGCAACTTT
TTTTTCTATTTTTACTTCGTCACACATTTTTAATCAGCGCAAGACTCTCT
CTTCACACATTTCTCTCGCCATGTTTTCGTTAAACAGAAGCGCTTTTCTG
AATAATCCTGCGAGCATATCAGGCTCGTTCCCTGATAAGCATAGAAGAAT
ACTGCCTACAAGAGTGGTGGTTGCGGGCTCGTCTAACAGAGATAAGAGTA
ACGGTAGTCTTTTAATGGCAACCGATGAAAGCACAGCTACATTAAAAGAA
ATGAGAGATGGCTATGCCGAAGTTGTGGACCCCAAGTCCGGCGTCAGCGG
TGGGGTCCAGGACGTTTATGGTGAGGATACCGCCACCGAAGACCAGCCGG
TCACCCCTTGGTCTGTCTCAGTTGCTAGTGGGTATTCTTTGTTGAGAGAT
CCACACCACAACAAAGGGCTTGCCTTCTCTGAAAAAGAAAGGAATTCACA
TTACTTGCGTGGTCTTCTCCCCCCTACAGTTATATCCCAAGAGCTCCAGG
TGAAGAAAATGTTGCATAATATTCGGCAGTACCAAGTTCCACTCCAGAAG
TACATGGCCATGATGGATCTTCAGGAGAGAAATCAGAAACTGTTCTATAA
GCTTCTCATTGACAATGTTGAGGAGTTACTCCCAATTGTCTATACTCCAA
CTGTTGGAGAAGCTTGCCAGAAGTACGGGAGCATCTATAGTCGTCCTCAG
GGTGTTTTTATCAGTTTGAAGGATAAGGGCAAGGTTCTTGAGGTATTGAG
GAACTGGCCTGAGAAGAATATTCAAGTCATTGTTGTCACT
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