CK936525

Overview
NameCK936525
Unique NameCK936525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length840
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO7I (NAD-dependent malic enzyme OS=Escherichia coli O7:K1 (strain IAI39 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO5E (NAD-dependent malic enzyme OS=Escherichia coli O157:H7 (strain EC4115 / EHEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO57 (NAD-dependent malic enzyme OS=Escherichia coli O157:H7 GN=sfcA PE=3 SV=2)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO55 (NAD-dependent malic enzyme OS=Escherichia coli (strain 55989 / EAEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO45 (NAD-dependent malic enzyme OS=Escherichia coli O45:K1 (strain S88 / ExPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO27 (NAD-dependent malic enzyme OS=Escherichia coli O127:H6 (strain E2348/69 / EPEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ECO24 (NAD-dependent malic enzyme OS=Escherichia coli O139:H28 (strain E24377A / ETEC) GN=sfcA PE=3 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 1.062e-24
Identity = 55/138 (39.86%), Postives = 94/138 (68.12%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G  LL  P  NKG AFS +ER +  L GLLP  V + E Q ++     + ++  + K++ + ++Q+ N+ LFY+L+ ++++E++P++YTPTVG AC+++  IY R +GVFIS +++  + ++L+N P  NI+VIVVT
Sbjct:   16 AGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_VIBVU (NAD-dependent malic enzyme OS=Vibrio vulnificus GN=sfcA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 53/138 (38.41%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS +ER S  L GLLP T  + + QV++     + ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+F+S  ++ ++ ++L N    N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSAEERISFNLEGLLPETTETIQEQVERAYMQYKAFESDMDKHIYLRNIQDTNETLFYRLVQNHITEMMPIIYTPTVGAACENFSNIYRRGRGLFVSYANRDRIDDILNNASNHNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_VIBFM (NAD-dependent malic enzyme OS=Vibrio fischeri (strain MJ11) GN=sfcA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 54/138 (39.13%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS  ER    L GLLP  + S E Q  +     + ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+FIS ++K ++ ++L N   +N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSTTERKYFNLEGLLPEAIESIEEQTGRAYKQYQSFENDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYENKNRIDDLLNNAANQNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_VIBF1 (NAD-dependent malic enzyme OS=Vibrio fischeri (strain ATCC 700601 / ES114) GN=sfcA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 54/138 (39.13%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS  ER    L GLLP  + S E Q  +     + ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+FIS ++K ++ ++L N   +N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSTTERKYFNLEGLLPEAIESIEEQTGRAYKQYQNFENDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYENKNRIDDLLNNAANQNVKVIVVT 150
The following BLAST results are available for this feature:
BLAST of CK936525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_ECO7I1.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O7:... [more]
MAO1_ECO5E1.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O15... [more]
MAO1_ECO571.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O15... [more]
MAO1_ECO551.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli (st... [more]
MAO1_ECO451.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O45... [more]
MAO1_ECO271.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O12... [more]
MAO1_ECO241.062e-2439.86NAD-dependent malic enzyme OS=Escherichia coli O13... [more]
MAO1_VIBVU1.387e-2438.41NAD-dependent malic enzyme OS=Vibrio vulnificus GN... [more]
MAO1_VIBFM1.387e-2439.13NAD-dependent malic enzyme OS=Vibrio fischeri (str... [more]
MAO1_VIBF11.387e-2439.13NAD-dependent malic enzyme OS=Vibrio fischeri (str... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004521_C09 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IVF_C09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936525 ID=CK936525; Name=CK936525; organism=Citrus sinensis; type=EST; length=840bp
GGGGAGACCCNNNAGACTCTCCCTTCTCTTCTCATTGCCTCTGCAACTTT
TTTTTCTATTTTTACTTCGTCACACATTTTTAATCAGCGCAAGACTCTCT
CTTCACACATTTCTCTCGCCATGTTTTCGTTAAACAGAAGCGCTTTTCTG
AATAATCCTGCGAGCATATCAGGCTCGTTCCCTGATAAGCATAGAAGAAT
ACTGCCTACAAGAGTGGTGGTTGCGGGCTCGTCTAACAGAGATAAGAGTA
ACGGTAGTCTTTTAATGGCAACCGATGAAAGCACAGCTACATTAAAAGAA
ATGAGAGATGGCTATGCCGAAGTTGTGGACCCCAAGTCCGGCGTCAGCGG
TGGGGTCCAGGACGTTTATGGTGAGGATACCGCCACCGAAGACCAGCCGG
TCACCCCTTGGTCTGTCTCAGTTGCTAGTGGGTATTCTTTGTTGAGAGAT
CCACACCACAACAAAGGGCTTGCCTTCTCTGAAAAAGAAAGGAATTCACA
TTACTTGCGTGGTCTTCTCCCCCCTACAGTTATATCCCAAGAGCTCCAGG
TGAAGAAAATGTTGCATAATATTCGGCAGTACCAAGTTCCACTCCAGAAG
TACATGGCCATGATGGATCTTCAGGAGAGAAATCAGAAACTGTTCTATAA
GCTTCTCATTGACAATGTTGAGGAGTTACTCCCAATTGTCTATACTCCAA
CTGTTGGAGAAGCTTGCCAGAAGTACGGGAGCATCTATAGTCGTCCTCAG
GGTGTTTTTATCAGTTTGAAGGATAAGGGCAAGGTTCTTGAGGTATTGAG
GAACTGGCCTGAGAAGAATATTCAAGTCATTGTTGTCACT
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