CK936525

Overview
NameCK936525
Unique NameCK936525
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length840
Libraries
Library NameType
Developing fruit flavedo at 80 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PHOLL (NAD-dependent malic enzyme OS=Photorhabdus luminescens subsp. laumondii GN=sfcA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 57/138 (41.30%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            SG  LL  P  NKG AF+E+ER +  L GLLP  V + E Q ++       ++    K++ + ++Q+ N+ LFY+LL  ++ E++PI+YTPTVGEAC+ +  IY R +G+FIS  +K  + ++L+N  ++N++VIVVT
Sbjct:   16 SGPILLEFPLLNKGSAFTEEERRNFNLHGLLPEAVETIEEQAERAYRQYLDFKNDADKHIYLRNIQDTNETLFYRLLDAHLNEMMPIIYTPTVGEACEHFSDIYRRARGLFISHPNKAHIDDMLQNATKQNVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_BDEBA (NAD-dependent malic enzyme OS=Bdellovibrio bacteriovorus GN=sfcA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 58/145 (40.00%), Postives = 95/145 (65.52%), Query Frame = 1 
Query:  406 PWSVSVASGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            P+S +V    SLL     NKG AFSE+ER    L GL+P T+ + E Q +++    R  +  + K++ + +LQ+ N+ LFY L+++++ E++PI+YTP VGEAC+ +  IY   +G+FIS  +K ++ E+LRN  ++ ++VIVVT
Sbjct:   14 PYSGTVLLEMSLL-----NKGSAFSEEERTHLNLHGLVPQTIETIEEQAERVYSQYRLLKADIDKHVYLRNLQDTNETLFYHLVVNHLTEMMPIIYTPVVGEACEHFSEIYRASRGLFISYPNKDRIDEMLRNVTKQKVKVIVVT 153
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_ALISL (NAD-dependent malic enzyme OS=Aliivibrio salmonicida (strain LFI1238) GN=sfcA PE=3 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 54/138 (39.13%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS  ER    L GLLP  + S E Q  +     + ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+FIS ++K ++ ++L N   +N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSTTERKYFNLEGLLPEAIESIEEQTGRAYKQYQSFENDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYENKNRIDDLLNNAANQNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO11_VIBVY (NAD-dependent malic enzyme 1 OS=Vibrio vulnificus (strain YJ016) GN=sfcA1 PE=3 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 1.387e-24
Identity = 53/138 (38.41%), Postives = 93/138 (67.39%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS +ER S  L GLLP T  + + QV++     + ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+F+S  ++ ++ ++L N    N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSAEERISFNLEGLLPETTETIQEQVERAYMQYKAFESDMDKHIYLRNIQDTNETLFYRLVQNHITEMMPIIYTPTVGAACENFSNIYRRGRGLFVSYANRDRIDDILNNASNHNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PSEA7 (NAD-dependent malic enzyme OS=Pseudomonas aeruginosa (strain PA7) GN=sfcA PE=3 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 2.366e-24
Identity = 54/138 (39.13%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G SLL  P  NKG AFS +ER    L+GLLP  + + E Q ++           L +++ +  +Q+ N+ LF++LL +++EE+LPI+YTPTVG+ACQ++  IY   +G+FIS  D+ ++ ++LR+  + N++++VVT
Sbjct:   14 AGPSLLEMPLLNKGSAFSTQERIDFNLQGLLPHNIETIEEQTERAYSQYNLCNTDLDRHIFLRSIQDNNETLFFRLLEEHLEEMLPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVT 151
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_VIBCH (NAD-dependent malic enzyme OS=Vibrio cholerae GN=sfcA PE=3 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 3.090e-24
Identity = 54/138 (39.13%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS +ER S  L GLLP    + + QV +     R ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+FIS  ++ ++ ++L N    N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_VIBC3 (NAD-dependent malic enzyme OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sfcA PE=3 SV=2)

HSP 1 Score: 112.464 bits (280), Expect = 3.090e-24
Identity = 54/138 (39.13%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G +LL  P  NKG AFS +ER S  L GLLP    + + QV +     R ++  + K++ + ++Q+ N+ LFY+L+ +++ E++PI+YTPTVG AC+ + +IY R +G+FIS  ++ ++ ++L N    N++VIVVT
Sbjct:   13 AGPALLSTPLLNKGSAFSAEERASFNLEGLLPEATETIQEQVVRAYQQYRGFESDMDKHIYLRNIQDTNETLFYRLVQNHISEMMPIIYTPTVGAACENFSNIYRRGRGLFISYANRDRIDDLLNNAANHNVKVIVVT 150
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PSEAE (NAD-dependent malic enzyme OS=Pseudomonas aeruginosa GN=sfcA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 4.035e-24
Identity = 53/138 (38.41%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G SLL  P  NKG AFS +ER    L+GLLP  + + E Q ++           L +++ +  +Q+ N+ LF++LL +++EE++PI+YTPTVG+ACQ++  IY   +G+FIS  D+ ++ ++LR+  + N++++VVT
Sbjct:   14 AGPSLLEMPLLNKGSAFSTQERIDFNLQGLLPHNIETIEEQTERAYSQYNLCNTDLDRHIFLRSIQDNNETLFFRLLEEHLEEMMPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVT 151
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PSEAB (NAD-dependent malic enzyme OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sfcA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 4.035e-24
Identity = 53/138 (38.41%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G SLL  P  NKG AFS +ER    L+GLLP  + + E Q ++           L +++ +  +Q+ N+ LF++LL +++EE++PI+YTPTVG+ACQ++  IY   +G+FIS  D+ ++ ++LR+  + N++++VVT
Sbjct:   14 AGPSLLEMPLLNKGSAFSTQERIDFNLQGLLPHNIETIEEQTERAYSQYNLCNTDLDRHIFLRSIQDNNETLFFRLLEEHLEEMMPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVT 151
[Close]
BLAST of CK936525 vs. ExPASy Swiss-Prot
Match: MAO1_PSEA8 (NAD-dependent malic enzyme OS=Pseudomonas aeruginosa (strain LESB58) GN=sfcA PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 4.035e-24
Identity = 53/138 (38.41%), Postives = 91/138 (65.94%), Query Frame = 1 
Query:  427 SGYSLLRDPHHNKGLAFSEKERNSHYLRGLLPPTVISQELQVKKMLHNIRQYQVPLQKYMAMMDLQERNQKLFYKLLIDNVEELLPIVYTPTVGEACQKYGSIYSRPQGVFISLKDKGKVLEVLRNWPEKNIQVIVVT 840
            +G SLL  P  NKG AFS +ER    L+GLLP  + + E Q ++           L +++ +  +Q+ N+ LF++LL +++EE++PI+YTPTVG+ACQ++  IY   +G+FIS  D+ ++ ++LR+  + N++++VVT
Sbjct:   14 AGPSLLEMPLLNKGSAFSTQERIDFNLQGLLPHNIETIEEQTERAYSQYNLCNTDLDRHIFLRSIQDNNETLFFRLLEEHLEEMMPIIYTPTVGQACQEFSKIYRTHRGLFISYPDRERIDDILRSATKNNVKIVVVT 151
The following BLAST results are available for this feature:
BLAST of CK936525 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 140
Match NameE-valueIdentityDescription
MAO1_PHOLL1.387e-2441.30NAD-dependent malic enzyme OS=Photorhabdus lumines... [more]
MAO1_BDEBA1.387e-2440.00NAD-dependent malic enzyme OS=Bdellovibrio bacteri... [more]
MAO1_ALISL1.387e-2439.13NAD-dependent malic enzyme OS=Aliivibrio salmonici... [more]
MAO11_VIBVY1.387e-2438.41NAD-dependent malic enzyme 1 OS=Vibrio vulnificus ... [more]
MAO1_PSEA72.366e-2439.13NAD-dependent malic enzyme OS=Pseudomonas aerugino... [more]
MAO1_VIBCH3.090e-2439.13NAD-dependent malic enzyme OS=Vibrio cholerae GN=s... [more]
MAO1_VIBC33.090e-2439.13NAD-dependent malic enzyme OS=Vibrio cholerae sero... [more]
MAO1_PSEAE4.035e-2438.41NAD-dependent malic enzyme OS=Pseudomonas aerugino... [more]
MAO1_PSEAB4.035e-2438.41NAD-dependent malic enzyme OS=Pseudomonas aerugino... [more]
MAO1_PSEA84.035e-2438.41NAD-dependent malic enzyme OS=Pseudomonas aerugino... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004521_C09 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IVF_C09 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK936525 ID=CK936525; Name=CK936525; organism=Citrus sinensis; type=EST; length=840bp
GGGGAGACCCNNNAGACTCTCCCTTCTCTTCTCATTGCCTCTGCAACTTT
TTTTTCTATTTTTACTTCGTCACACATTTTTAATCAGCGCAAGACTCTCT
CTTCACACATTTCTCTCGCCATGTTTTCGTTAAACAGAAGCGCTTTTCTG
AATAATCCTGCGAGCATATCAGGCTCGTTCCCTGATAAGCATAGAAGAAT
ACTGCCTACAAGAGTGGTGGTTGCGGGCTCGTCTAACAGAGATAAGAGTA
ACGGTAGTCTTTTAATGGCAACCGATGAAAGCACAGCTACATTAAAAGAA
ATGAGAGATGGCTATGCCGAAGTTGTGGACCCCAAGTCCGGCGTCAGCGG
TGGGGTCCAGGACGTTTATGGTGAGGATACCGCCACCGAAGACCAGCCGG
TCACCCCTTGGTCTGTCTCAGTTGCTAGTGGGTATTCTTTGTTGAGAGAT
CCACACCACAACAAAGGGCTTGCCTTCTCTGAAAAAGAAAGGAATTCACA
TTACTTGCGTGGTCTTCTCCCCCCTACAGTTATATCCCAAGAGCTCCAGG
TGAAGAAAATGTTGCATAATATTCGGCAGTACCAAGTTCCACTCCAGAAG
TACATGGCCATGATGGATCTTCAGGAGAGAAATCAGAAACTGTTCTATAA
GCTTCTCATTGACAATGTTGAGGAGTTACTCCCAATTGTCTATACTCCAA
CTGTTGGAGAAGCTTGCCAGAAGTACGGGAGCATCTATAGTCGTCCTCAG
GGTGTTTTTATCAGTTTGAAGGATAAGGGCAAGGTTCTTGAGGTATTGAG
GAACTGGCCTGAGAAGAATATTCAAGTCATTGTTGTCACT
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