CK936495 | Citrus Genome Database

Overview


Name	CK936495
Unique Name	CK936495
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	754

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACTS_ORYLA (Actin, alpha skeletal muscle OS=Oryzias latipes GN=acta1 PE=2 SV=1)

HSP 1 Score: 366.696 bits (940), Expect = 6.528e-105
Identity = 180/201 (89.55%), Postives = 188/201 (93.53%), Query Frame = 1

Query:  163 NG*GWV----AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            NG G V    AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVL LYASGRTTGIVLD+GDGV+H VP+YEGYALPHAI+RLDLAGRDLTD LMKILTERGYSF TTAEREIVRD+
Sbjct:   14 NGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDAGDGVTHNVPVYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDI 214

HSP 2 Score: 34.2686 bits (77), Expect = 6.528e-105
Identity = 14/22 (63.64%), Postives = 17/22 (77.27%), Query Frame = 2

Query:  113 MADGEDIQPLVCDNGTGMVKAG 178
            M D E+   LVCDNG+G+VKAG
Sbjct:    1 MCDDEETTALVCDNGSGLVKAG 22

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACTC_XENLA (Actin, alpha cardiac muscle 1 OS=Xenopus laevis GN=actc1 PE=2 SV=1)

HSP 1 Score: 366.696 bits (940), Expect = 6.528e-105
Identity = 182/201 (90.55%), Postives = 187/201 (93.03%), Query Frame = 1

Query:  163 NG*GWV----AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            NG G V    AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVL LYASGRTTGIVLDSGDGV+H VPIYEGYALPHAI RLDLAGRDLTD LMKILTERGYSF TTAEREIVRD+
Sbjct:   14 NGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIQRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDI 214

HSP 2 Score: 34.2686 bits (77), Expect = 6.528e-105
Identity = 14/22 (63.64%), Postives = 17/22 (77.27%), Query Frame = 2

Query:  113 MADGEDIQPLVCDNGTGMVKAG 178
            M D E+   LVCDNG+G+VKAG
Sbjct:    1 MCDDEETTALVCDNGSGLVKAG 22

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT1_ARTSX (Actin, clone 205 OS=Artemia sp. PE=2 SV=1)

HSP 1 Score: 374.785 bits (961), Expect = 6.528e-105
Identity = 181/191 (94.76%), Postives = 188/191 (98.43%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHP+LLTEAPLNPKANREKMTQIMFETFN+PAMYVAIQAVL LYASGRTTG+VLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGYSFTTTAEREIVRD+
Sbjct:   23 AGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGVVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213

HSP 2 Score: 26.1794 bits (56), Expect = 6.528e-105
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  125 EDIQPLVCDNGTGMVKAG 178
            +++  LV DNG+GM KAG
Sbjct:    4 DEVAALVVDNGSGMCKAG 21

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT1_AEDAE (Actin-1 OS=Aedes aegypti GN=ACT-1 PE=2 SV=2)

HSP 1 Score: 373.629 bits (958), Expect = 6.528e-105
Identity = 182/191 (95.29%), Postives = 187/191 (97.91%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRH GVMVGMG KD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN+PAMYVAIQAVL LYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGYSFTTTAEREIVRD+
Sbjct:   23 AGDDAPRAVFPSIVGRPRHQGVMVGMGNKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213

HSP 2 Score: 27.335 bits (59), Expect = 6.528e-105
Identity = 12/18 (66.67%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  125 EDIQPLVCDNGTGMVKAG 178
            ED   LV DNG+GM KAG
Sbjct:    4 EDAAALVVDNGSGMCKAG 21

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT_TAESO (Actin OS=Taenia solium GN=ACT1 PE=3 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 6.528e-105
Identity = 181/191 (94.76%), Postives = 186/191 (97.38%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYV IQAVL LYASGRTTGIVLDSGDGV+H+VPIYEGYALPHAILRLDLAGRDLTD LMKILTERGYSFTTTAEREIVRD+
Sbjct:   23 AGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQAVLSLYASGRTTGIVLDSGDGVTHSVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213

HSP 2 Score: 29.6462 bits (65), Expect = 6.528e-105
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  125 EDIQPLVCDNGTGMVKAG 178
            E++Q LV DNG+GM KAG
Sbjct:    4 EEVQALVVDNGSGMCKAG 21

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT1_DIPDE (Actin-1/4 OS=Diphyllobothrium dendriticum GN=ACT1 PE=2 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 6.528e-105
Identity = 181/191 (94.76%), Postives = 186/191 (97.38%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYV IQAVL LYASGRTTGIVLDSGDGV+H+VPIYEGYALPHAILRLDLAGRDLTD LMKILTERGYSFTTTAEREIVRD+
Sbjct:   23 AGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQAVLSLYASGRTTGIVLDSGDGVTHSVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213

HSP 2 Score: 29.6462 bits (65), Expect = 6.528e-105
Identity = 12/18 (66.67%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  125 EDIQPLVCDNGTGMVKAG 178
            E++Q LV DNG+GM KAG
Sbjct:    4 EEVQALVVDNGSGMCKAG 21

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT2_SOLLC (Actin-51 (Fragment) OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 380.178 bits (975), Expect = 6.693e-105
Identity = 187/191 (97.91%), Postives = 187/191 (97.91%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAY GDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVL LYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGY FTTTAEREIVRDM
Sbjct:    4 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYDGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYMFTTTAEREIVRDM 194

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT2_BOMMO (Actin, muscle-type A2 OS=Bombyx mori PE=3 SV=1)

HSP 1 Score: 374.785 bits (961), Expect = 8.517e-105
Identity = 182/191 (95.29%), Postives = 187/191 (97.91%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHP+LLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVL LYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGYSFTTTAEREIVRD+
Sbjct:   23 AGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPILLTEAPLNPKANREKMTQIMFETFNCPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213

HSP 2 Score: 25.7942 bits (55), Expect = 8.517e-105
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  125 EDIQPLVCDNGTGMVKAG 178
            +D   LV DNG+GM KAG
Sbjct:    4 DDAGALVVDNGSGMCKAG 21

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACTS_ATRMM (Actin, alpha skeletal muscle OS=Atractaspis microlepidota microlepidota GN=ACTA1 PE=2 SV=1)

HSP 1 Score: 367.466 bits (942), Expect = 1.111e-104
Identity = 182/201 (90.55%), Postives = 188/201 (93.53%), Query Frame = 1

Query:  163 NG*GWV----AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            NG G V    AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPKANREKMTQIMFETFN PAMYVAIQAVL LYASGRTTGIVLDSGDGV+H VPIYEGYALPHAI+RLDLAGRDLTD LMKILTERGYSF TTAEREIVRD+
Sbjct:   14 NGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLPLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDI 214

HSP 2 Score: 32.7278 bits (73), Expect = 1.111e-104
Identity = 13/22 (59.09%), Postives = 17/22 (77.27%), Query Frame = 2

Query:  113 MADGEDIQPLVCDNGTGMVKAG 178
            M D ++   LVCDNG+G+VKAG
Sbjct:    1 MCDEDETTALVCDNGSGLVKAG 22

[Close]

BLAST of CK936495 vs. ExPASy Swiss-Prot
Match: ACT5_BACDO (Actin-5, muscle-specific OS=Bactrocera dorsalis PE=2 SV=1)

HSP 1 Score: 375.17 bits (962), Expect = 1.111e-104
Identity = 182/191 (95.29%), Postives = 188/191 (98.43%), Query Frame = 1

Query:  181 AGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNAPAMYVAIQAVLFLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDNLMKILTERGYSFTTTAEREIVRDM 753
            AGDDAPRAVFPSIVGRPRH GVMVGMGQKD+YVGDEAQSKRGILTLKYPIEHGI++NWDDMEK+WHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFN+PAMYVAIQAVL LYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTD LMKILTERGYSFTTTAEREIVRD+
Sbjct:   23 AGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKVWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDI 213

HSP 2 Score: 25.0238 bits (53), Expect = 1.111e-104
Identity = 10/18 (55.56%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  125 EDIQPLVCDNGTGMVKAG 178
            ++   LV DNG+GM KAG
Sbjct:    4 DEASALVVDNGSGMCKAG 21

The following BLAST results are available for this feature:

BLAST of CK936495 vs. ExPASy Swiss-Prot

BLAST of CK936495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 458


Match Name	E-value	Identity	Description
ACTS_ORYLA	6.528e-105	89.55	Actin, alpha skeletal muscle OS=Oryzias latipes GN...	[more]
ACTC_XENLA	6.528e-105	90.55	Actin, alpha cardiac muscle 1 OS=Xenopus laevis GN...	[more]
ACT1_ARTSX	6.528e-105	94.76	Actin, clone 205 OS=Artemia sp. PE=2 SV=1	[more]
ACT1_AEDAE	6.528e-105	95.29	Actin-1 OS=Aedes aegypti GN=ACT-1 PE=2 SV=2	[more]
ACT_TAESO	6.528e-105	94.76	Actin OS=Taenia solium GN=ACT1 PE=3 SV=1	[more]
ACT1_DIPDE	6.528e-105	94.76	Actin-1/4 OS=Diphyllobothrium dendriticum GN=ACT1 ...	[more]
ACT2_SOLLC	6.693e-105	97.91	Actin-51 (Fragment) OS=Solanum lycopersicum PE=3 S...	[more]
ACT2_BOMMO	8.517e-105	95.29	Actin, muscle-type A2 OS=Bombyx mori PE=3 SV=1	[more]
ACTS_ATRMM	1.111e-104	90.55	Actin, alpha skeletal muscle OS=Atractaspis microl...	[more]
ACT5_BACDO	1.111e-104	95.29	Actin-5, muscle-specific OS=Bactrocera dorsalis PE...	[more]

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Properties


Property Name	Value
Genbank description	CGF1004521_G10 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IVF_G10 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936495 ID=CK936495; Name=CK936495; organism=Citrus sinensis; type=EST; length=754bp
GCGGGGAATCAANNNACAACAAAAACCAAACTAAAACAAAACAAAACAAG
GAGGCGTCCATTTTGTGCGCCTCGTAATTCGCTCGTTCTCATCTCATAAC
CGGTAGCAGAAGATGGCAGACGGTGAGGATATTCAGCCACTTGTTTGTGA
TAATGGAACAGGAATGGTTAAGGCTGGGTTGCCGGAGATGATGCTCCACG
GGCGGTGTTCCCCAGTATTGTTGGTCGTCCACGTCACACTGGTGTGATGG
TTGGCATGGGTCAGAAGGATGCGTATGTTGGTGATGAGGCTCAATCGAAG
AGAGGTATTTTGACTTTGAAATACCCCATTGAGCATGGTATTGTGAGCAA
CTGGGATGACATGGAGAAGATCTGGCATCACACCTTCTACAATGAGCTTC
GTGTTGCCCCTGAAGAGCACCCTGTTCTTCTTACTGAGGCTCCTCTCAAT
CCGAAGGCTAATCGCGAGAAGATGACCCAGATTATGTTTGAGACCTTTAA
TGCTCCCGCTATGTATGTTGCCATCCAGGCCGTTCTTTTCTTGTATGCGA
GTGGTCGTACCACTGGTATTGTGTTGGACTCTGGTGATGGTGTCAGCCAT
ACAGTTCCTATATATGAGGGTTATGCCCTCCCACATGCAATACTTCGTCT
TGACCTTGCTGGTCGTGACCTCACTGATAACTTGATGAAAATCCTCACTG
AGCGTGGGTACTCTTTCACCACCACGGCGGAGCGTGAAATTGTCAGGGAC
ATGA