CN186521

Overview
NameCN186521
Unique NameCN186521
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length658
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN2_ORYSJ (GTP-binding nuclear protein Ran-2 OS=Oryza sativa subsp. japonica GN=RAN2 PE=2 SV=1)

HSP 1 Score: 392.504 bits (1007), Expect = 1.013e-108
Identity = 185/193 (95.85%), Postives = 188/193 (97.41%), Query Frame = 1 
Query:   79 MALPSQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            MALP+Q TVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDF TNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVT+RLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVE+ AL PPEV ID
Sbjct:    1 MALPNQGTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVEAVALKPPEVPID 193
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN2_ORYSI (GTP-binding nuclear protein Ran-2 OS=Oryza sativa subsp. indica GN=RAN2 PE=2 SV=1)

HSP 1 Score: 392.504 bits (1007), Expect = 1.013e-108
Identity = 185/193 (95.85%), Postives = 188/193 (97.41%), Query Frame = 1 
Query:   79 MALPSQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            MALP+Q TVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDF TNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVT+RLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVE+ AL PPEV ID
Sbjct:    1 MALPNQGTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFTTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTSRLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVEAVALKPPEVPID 193
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN1B_LOTJA (GTP-binding nuclear protein Ran1B (Fragment) OS=Lotus japonicus GN=RAN1B PE=2 SV=1)

HSP 1 Score: 384.03 bits (985), Expect = 3.601e-106
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 1 
Query:  115 SFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            +FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID
Sbjct:    1 NFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 181
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN1A_LOTJA (GTP-binding nuclear protein Ran1A (Fragment) OS=Lotus japonicus GN=RAN1A PE=2 SV=1)

HSP 1 Score: 384.03 bits (985), Expect = 3.601e-106
Identity = 180/181 (99.45%), Postives = 181/181 (100.00%), Query Frame = 1 
Query:  115 SFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            +FKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID
Sbjct:    1 NFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 181
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN3_ORYSJ (GTP-binding nuclear protein Ran-3 OS=Oryza sativa subsp. japonica GN=RAN3 PE=2 SV=1)

HSP 1 Score: 346.665 bits (888), Expect = 6.371e-95
Identity = 161/196 (82.14%), Postives = 175/196 (89.29%), Query Frame = 1 
Query:   70 TQLMALPSQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            ++  ALP    V YPSFKL++VGDGGTGKTTFVKRH+TGEFEK+YEPTIGVEV PLDF T+ GK+RF CWDTAGQEKFGGLRDGYYIHG CAIIMFDVT+RLTYKNVPTWH+D+CRVC+NIPIVLCGNKVD+KNRQVKAK VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL GD NL FVE  AL P +V ID
Sbjct:    2 SRAQALPDPAAVGYPSFKLILVGDGGTGKTTFVKRHITGEFEKRYEPTIGVEVRPLDFHTSRGKVRFCCWDTAGQEKFGGLRDGYYIHGHCAIIMFDVTSRLTYKNVPTWHKDICRVCDNIPIVLCGNKVDMKNRQVKAKMVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLTGDMNLRFVEELALLPADVTID 197
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN3_ORYSI (GTP-binding nuclear protein Ran-3 OS=Oryza sativa subsp. indica GN=RAN3 PE=2 SV=2)

HSP 1 Score: 346.665 bits (888), Expect = 6.371e-95
Identity = 161/196 (82.14%), Postives = 175/196 (89.29%), Query Frame = 1 
Query:   70 TQLMALPSQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            ++  ALP    V YPSFKL++VGDGGTGKTTFVKRH+TGEFEK+YEPTIGVEV PLDF T+ GK+RF CWDTAGQEKFGGLRDGYYIHG CAIIMFDVT+RLTYKNVPTWH+D+CRVC+NIPIVLCGNKVD+KNRQVKAK VTFHRKKNLQYYEISAKSNYNFEKPFLYLARKL GD NL FVE  AL P +V ID
Sbjct:    2 SRAQALPDPAAVGYPSFKLILVGDGGTGKTTFVKRHITGEFEKRYEPTIGVEVRPLDFHTSRGKVRFCCWDTAGQEKFGGLRDGYYIHGHCAIIMFDVTSRLTYKNVPTWHKDICRVCDNIPIVLCGNKVDMKNRQVKAKMVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLTGDMNLRFVEELALLPADVTID 197
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: GSP2_YEAST (GTP-binding nuclear protein GSP2/CNR2 OS=Saccharomyces cerevisiae GN=GSP2 PE=1 SV=1)

HSP 1 Score: 336.65 bits (862), Expect = 6.593e-92
Identity = 157/193 (81.35%), Postives = 174/193 (90.16%), Query Frame = 1 
Query:   79 MALPSQQTVDYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            M+ P+Q   + P+FKLV+VGDGGTGKTTFVKRHLTGEFEKKY  TIGVEVHPL F+TN G+I+F  WDTAGQEKFGGLRDGYYI+ QCAIIMFDVT+R+TYKNVP WHRDL RVCENIPIVLCGNKVDVK R+VKAK +TFHRKKNLQYY+ISAKSNYNFEKPFL+LARKLAG+P L FV SPALAPPEVQ+D
Sbjct:    1 MSAPAQNNAEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVASPALAPPEVQVD 193
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: RAN_CAEEL (GTP-binding nuclear protein ran-1 OS=Caenorhabditis elegans GN=ran-1 PE=1 SV=1)

HSP 1 Score: 330.487 bits (846), Expect = 4.725e-90
Identity = 156/182 (85.71%), Postives = 167/182 (91.76%), Query Frame = 1 
Query:  112 PSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            P+FKLV+VGDGGTGKTTFVKRHLTGEFEKKY  T+GVEVHPL F TN G+IRF  WDTAGQEKFGGLRDGYYI GQCAIIMFDVTAR+TYKNVP WHRDL RVCENIPIVLCGNKVDVK+R+VKAK +TFHRKKNLQYY+ISAKSNYNFEKPFL+LARKL GDPNL FV  PALAPPEVQ+D
Sbjct:    8 PTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGQIRFNVWDTAGQEKFGGLRDGYYIQGQCAIIMFDVTARVTYKNVPNWHRDLARVCENIPIVLCGNKVDVKDRKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLLGDPNLEFVAMPALAPPEVQMD 189
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: GSP1_YEAST (GTP-binding nuclear protein GSP1/CNR1 OS=Saccharomyces cerevisiae GN=GSP1 PE=1 SV=1)

HSP 1 Score: 329.717 bits (844), Expect = 8.060e-90
Identity = 154/184 (83.70%), Postives = 169/184 (91.85%), Query Frame = 1 
Query:  106 DYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            + P+FKLV+VGDGGTGKTTFVKRHLTGEFEKKY  TIGVEVHPL F+TN G+I+F  WDTAGQEKFGGLRDGYYI+ QCAIIMFDVT+R+TYKNVP WHRDL RVCENIPIVLCGNKVDVK R+VKAK +TFHRKKNLQYY+ISAKSNYNFEKPFL+LARKLAG+P L FV SPALAPPEVQ+D
Sbjct:    9 EVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLEFVASPALAPPEVQVD 192
[Close]
BLAST of CN186521 vs. ExPASy Swiss-Prot
Match: GSP1_ASHGO (GTP-binding nuclear protein GSP1/Ran OS=Ashbya gossypii GN=GSP1 PE=3 SV=1)

HSP 1 Score: 327.02 bits (837), Expect = 5.224e-89
Identity = 153/184 (83.15%), Postives = 168/184 (91.30%), Query Frame = 1 
Query:  106 DYPSFKLVIVGDGGTGKTTFVKRHLTGEFEKKYEPTIGVEVHPLDFFTNCGKIRFYCWDTAGQEKFGGLRDGYYIHGQCAIIMFDVTARLTYKNVPTWHRDLCRVCENIPIVLCGNKVDVKNRQVKAKQVTFHRKKNLQYYEISAKSNYNFEKPFLYLARKLAGDPNLHFVESPALAPPEVQID 657
            + P+FKLV+VGDGGTGKTTFVKRHLTGEFEKKY  TIGVEVHPL F+TN G+I+F  WDTAGQEKFGGLRDGYYI+ QC IIMFDVT+R+TYKNVP WHRDL RVCENIPIVLCGNKVDVK R+VKAK +TFHRKKNLQYY+ISAKSNYNFEKPFL+LARKLAG+P L FV SPALAPPEVQ+D
Sbjct:    4 EVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATIGVEVHPLAFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCGIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLAGNPQLDFVASPALAPPEVQVD 187
The following BLAST results are available for this feature:
BLAST of CN186521 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
RAN2_ORYSJ1.013e-10895.85GTP-binding nuclear protein Ran-2 OS=Oryza sativa ... [more]
RAN2_ORYSI1.013e-10895.85GTP-binding nuclear protein Ran-2 OS=Oryza sativa ... [more]
RAN1B_LOTJA3.601e-10699.45GTP-binding nuclear protein Ran1B (Fragment) OS=Lo... [more]
RAN1A_LOTJA3.601e-10699.45GTP-binding nuclear protein Ran1A (Fragment) OS=Lo... [more]
RAN3_ORYSJ6.371e-9582.14GTP-binding nuclear protein Ran-3 OS=Oryza sativa ... [more]
RAN3_ORYSI6.371e-9582.14GTP-binding nuclear protein Ran-3 OS=Oryza sativa ... [more]
GSP2_YEAST6.593e-9281.35GTP-binding nuclear protein GSP2/CNR2 OS=Saccharom... [more]
RAN_CAEEL4.725e-9085.71GTP-binding nuclear protein ran-1 OS=Caenorhabditi... [more]
GSP1_YEAST8.060e-9083.70GTP-binding nuclear protein GSP1/CNR1 OS=Saccharom... [more]
GSP1_ASHGO5.224e-8983.15GTP-binding nuclear protein GSP1/Ran OS=Ashbya gos... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0003A14_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0003A14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186521 ID=CN186521; Name=CN186521; organism=Citrus sinensis; type=EST; length=658bp
ATTCTCTCTCTCTCTCTCTCGCTCACTTCTGTGTTCAGACCGCGAACTTC
GTATTCTTTCGCACACAGTACTCAACTCATGGCTCTGCCAAGTCAGCAAA
CTGTTGATTACCCGAGCTTCAAACTCGTCATAGTCGGCGACGGTGGCACT
GGGAAGACTACCTTTGTGAAGAGGCATCTGACTGGGGAGTTTGAGAAGAA
GTATGAACCAACTATTGGTGTGGAGGTTCATCCGTTGGATTTCTTTACAA
ATTGTGGCAAAATCCGTTTCTACTGCTGGGACACTGCTGGCCAGGAAAAG
TTTGGTGGTCTTAGAGATGGTTATTACATCCATGGGCAATGTGCCATCAT
TATGTTTGATGTTACTGCCCGATTGACATACAAGAATGTTCCCACTTGGC
ACCGTGATCTTTGCCGGGTGTGTGAAAATATACCGATTGTTCTCTGTGGG
AACAAGGTTGATGTGAAGAATAGGCAAGTCAAGGCAAAGCAGGTTACCTT
CCACAGGAAGAAGAATCTACAGTACTATGAGATTTCTGCCAAGAGCAACT
ACAATTTTGAGAAACCTTTCTTGTACCTTGCCAGGAAGCTTGCTGGGGAC
CCAAATTTGCACTTCGTGGAATCTCCTGCTCTCGCTCCTCCAGAAGTACA
GATTGACT
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